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Reviewed, UniProtKB/Swiss-Prot P50545 (HCK_RAT)

Last modified February 9, 2010. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Tyrosine-protein kinase HCK
    EC=2.7.10.2
Alternative name(s):
    Hemopoietic cell kinase
    p56-HCK
Gene names
Name: Hck
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location

Membrane; Lipid-anchor.

Tissue specificity

Expressed strongly in spleen and at very low levels in thymus. Ref.2

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Contains 1 protein kinase domain.

Contains 1 SH2 domain.

Contains 1 SH3 domain.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform p59-HCK (identifier: P50545-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform p56-HCK (identifier: P50545-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.
Note: Initiator Met-1 is removed. Contains a N-myristoyl glycine at position 3 (By similarity). Contains a S-palmitoyl cysteine at position 4 (By similarity).

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 524523Tyrosine-protein kinase HCK
PRO_0000088104

Regions

Domain76 – 13661SH3
Domain142 – 23998SH2
Domain260 – 513254Protein kinase
Nucleotide binding266 – 2749ATP By similarity

Sites

Active site3791Proton acceptor By similarity
Binding site2881ATP By similarity

Amino acid modifications

Modified residue341Phosphoserine By similarity
Modified residue501Phosphotyrosine By similarity
Modified residue1251Phosphotyrosine By similarity
Modified residue2001Phosphothreonine By similarity
Modified residue2071Phosphotyrosine By similarity
Modified residue4091Phosphotyrosine; by autocatalysis By similarity
Modified residue4601Phosphoserine By similarity
Modified residue5131Phosphotyrosine By similarity
Modified residue5181Phosphoserine By similarity
Modified residue5201Phosphotyrosine By similarity
Lipidation21N-myristoyl glycine By similarity

Natural variations

Alternative sequence1 – 2121Missing in isoform p56-HCK.
VSP_022248

Experimental info

Sequence conflict721V → F in AAA41312. Ref.1
Sequence conflict721V → F in AAB20754. Ref.1
Sequence conflict2261K → R in CAA44218. Ref.2
Sequence conflict3271I → T in CAA44218. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform p59-HCK [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: BBD928C9E717BF4B

FASTA52459,154
        10         20         30         40         50         60 
MGGRSSCEDP GCPRGEGRVP RMGCVKSRFL REGSKASKIE PNANQKGPVY VPDPTSPKKL 

        70         80         90        100        110        120 
GPNSINSLPP GVVEGSEDTI VVALYDYEAI HREDLSFQKG DQMVVLEESG EWWKARSLAT 

       130        140        150        160        170        180 
KKEGYIPSNY VARVNSLETE EWFFKGISRK DAERHLLAPG NMLGSFMIRD SETTKGSYSL 

       190        200        210        220        230        240 
SVRDFDPQHG DTVKHYKIRT LDSGGFYISP RSTFSSLQEL VVHYKKGKDG LCQKLSVPCV 

       250        260        270        280        290        300 
SPKPQKPWEK DAWEIPRESL QMEKKLGAGQ FGEVWMATYN KHTKVAVKTM KPGSMSVEAF 

       310        320        330        340        350        360 
LAEANLMKTL QHDKLVKLHA VVSQEPIFIV TEFMAKGSLL DFLKSEEGSK QPLPKLIDFS 

       370        380        390        400        410        420 
AQISEGMAFI EQRNYIHRDL RAANILVSAS LVCKIADFGL ARIIEDNEYT AREGAKFPIK 

       430        440        450        460        470        480 
WTAPEAINFG SFTIKSDVWS FGILLMEIVT YGRIPYPGMS NPEVIRALEH GYRMPRPDNC 

       490        500        510        520 
PEELYSIMIR CWKNRPEERP TFEYIQSVLD DFYTATESQY QQQP 

« Hide

Isoform p56-HCK.

Checksum: 4D4D0777FF3AAC99
Show »

FASTA50356,968

References

« Hide 'large scale' references
[1]"Identification of rat cDNA encoding hck tyrosine kinase from megakaryocytes."
Okano Y., Sugimoto Y., Fukuoka M., Matsui A., Nagata K., Nozawa Y.
Biochem. Biophys. Res. Commun. 181:1137-1144(1991) [PubMed: 1764064] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Megakaryocyte.
[2]"Nucleotide sequence of a cDNA coding for rat hck tyrosine kinase and characterization of its gene product."
Vijaya Gouri B.S., Rema V., Kamatkar S., Swarup G.
J. Biosci. 19:117-129(1994)
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Wistar.
Tissue: Spleen.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Brown Norway.
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M83666 mRNA. Translation: AAA41312.1. Different initiation.
S74141 mRNA. Translation: AAB20754.1. Different initiation.
X62345 mRNA. Translation: CAA44218.1. Different initiation.
BC078890 mRNA. Translation: AAH78890.2.
IPIIPI00325226.
IPI00817068.
PIRJQ1321.
RefSeqNP_037317.2.
UniGeneRn.10945

3D structure databases

SMRP50545. Positions 80-524.
ModBaseSearch...

Protein-protein interaction databases

STRINGP50545.

PTM databases

PhosphoSiteP50545.

Genome annotation databases

EnsemblENSRNOT00000012432; ENSRNOP00000012432; ENSRNOG00000009331; Rattus norvegicus. [Genome view]
GeneID25734.
KEGGrno:25734.
UCSCBC078890. rat.

Organism-specific databases

CTD25734.
RGD2785. Hck.

Phylogenomic databases

eggNOGmaNOG15300.
HOVERGENP50545.
InParanoidP50545.

Enzyme and pathway databases

BRENDA2.7.10.2. 248.

Gene expression databases

ArrayExpressP50545.
GenevestigatorP50545.
GermOnlineENSRNOG00000009331. Rattus norvegicus.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR020473. SH3_region.
IPR020748. Tyr_kinase_non-rcpt_Hck.
IPR020635. Tyr_Pkinase_cat_dom.
IPR020685. Tyr_prot_kinase.
IPR008266. Tyr_prot_kinase_AS.
[Graphical view]
Gene3DG3DSA:3.30.505.10. SH2. 1 hit.
PANTHERPTHR23256:SF259. Tyr_kinase_non-rcpt_Hck. 1 hit.
PTHR23256. Tyr_prot_kinase. 1 hit.
PfamPF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio607867.

Entry information

Entry nameHCK_RAT
AccessionPrimary (citable) accession number: P50545
Secondary accession number(s): Q64647, Q6AYV7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: February 9, 2010
This is version 98 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents