Reviewed, UniProtKB/Swiss-Prot P50545 (HCK_RAT)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase HCK
    EC=2.7.10.2
Alternative name(s):
    Hemopoietic cell kinase
    p56-HCK

Gene names

Name:

Hck

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Lipid-anchor.
Tissue specificity	Expressed strongly in spleen and at very low levels in thymus. Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative initiation
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Lipoprotein Myristate Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform p59-HCK (identifier: P50545-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform p56-HCK (identifier: P50545-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.

Note: Initiator Met-1 is removed. Contains a N-myristoyl glycine at position 3 (By similarity). Contains a S-palmitoyl cysteine at position 4 (By similarity).

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 524	523	Tyrosine-protein kinase HCK		PRO_0000088104
Regions
	Domain	76 – 136	61	SH3
	Domain	142 – 239	98	SH2
	Domain	260 – 513	254	Protein kinase
	Nucleotide binding	266 – 274	9	ATP By similarity
Sites
	Active site	379	1	Proton acceptor By similarity
	Binding site	288	1	ATP By similarity
Amino acid modifications
	Modified residue	34	1	Phosphoserine By similarity
	Modified residue	50	1	Phosphotyrosine By similarity
	Modified residue	125	1	Phosphotyrosine By similarity
	Modified residue	200	1	Phosphothreonine By similarity
	Modified residue	207	1	Phosphotyrosine By similarity
	Modified residue	409	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	460	1	Phosphoserine By similarity
	Modified residue	513	1	Phosphotyrosine By similarity
	Modified residue	518	1	Phosphoserine By similarity
	Modified residue	520	1	Phosphotyrosine By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity
Natural variations
	Alternative sequence	1 – 21	21	Missing in isoform p56-HCK.		VSP_022248
Experimental info
	Sequence conflict	72	1	V → F in AAA41312. Ref.1
	Sequence conflict	72	1	V → F in AAB20754. Ref.1
	Sequence conflict	226	1	K → R in CAA44218. Ref.2
	Sequence conflict	327	1	I → T in CAA44218. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform p59-HCK [UniParc]. Last modified January 23, 2007. Version 4. Checksum: BBD928C9E717BF4B	FASTA	524	59,154
	10 20 30 40 50 60 MGGRSSCEDP GCPRGEGRVP RMGCVKSRFL REGSKASKIE PNANQKGPVY VPDPTSPKKL 70 80 90 100 110 120 GPNSINSLPP GVVEGSEDTI VVALYDYEAI HREDLSFQKG DQMVVLEESG EWWKARSLAT 130 140 150 160 170 180 KKEGYIPSNY VARVNSLETE EWFFKGISRK DAERHLLAPG NMLGSFMIRD SETTKGSYSL 190 200 210 220 230 240 SVRDFDPQHG DTVKHYKIRT LDSGGFYISP RSTFSSLQEL VVHYKKGKDG LCQKLSVPCV 250 260 270 280 290 300 SPKPQKPWEK DAWEIPRESL QMEKKLGAGQ FGEVWMATYN KHTKVAVKTM KPGSMSVEAF 310 320 330 340 350 360 LAEANLMKTL QHDKLVKLHA VVSQEPIFIV TEFMAKGSLL DFLKSEEGSK QPLPKLIDFS 370 380 390 400 410 420 AQISEGMAFI EQRNYIHRDL RAANILVSAS LVCKIADFGL ARIIEDNEYT AREGAKFPIK 430 440 450 460 470 480 WTAPEAINFG SFTIKSDVWS FGILLMEIVT YGRIPYPGMS NPEVIRALEH GYRMPRPDNC 490 500 510 520 PEELYSIMIR CWKNRPEERP TFEYIQSVLD DFYTATESQY QQQP
	Isoform p56-HCK. Checksum: 4D4D0777FF3AAC99	FASTA	503	56,968
	10 20 30 40 50 60 MGCVKSRFLR EGSKASKIEP NANQKGPVYV PDPTSPKKLG PNSINSLPPG VVEGSEDTIV 70 80 90 100 110 120 VALYDYEAIH REDLSFQKGD QMVVLEESGE WWKARSLATK KEGYIPSNYV ARVNSLETEE 130 140 150 160 170 180 WFFKGISRKD AERHLLAPGN MLGSFMIRDS ETTKGSYSLS VRDFDPQHGD TVKHYKIRTL 190 200 210 220 230 240 DSGGFYISPR STFSSLQELV VHYKKGKDGL CQKLSVPCVS PKPQKPWEKD AWEIPRESLQ 250 260 270 280 290 300 MEKKLGAGQF GEVWMATYNK HTKVAVKTMK PGSMSVEAFL AEANLMKTLQ HDKLVKLHAV 310 320 330 340 350 360 VSQEPIFIVT EFMAKGSLLD FLKSEEGSKQ PLPKLIDFSA QISEGMAFIE QRNYIHRDLR 370 380 390 400 410 420 AANILVSASL VCKIADFGLA RIIEDNEYTA REGAKFPIKW TAPEAINFGS FTIKSDVWSF 430 440 450 460 470 480 GILLMEIVTY GRIPYPGMSN PEVIRALEHG YRMPRPDNCP EELYSIMIRC WKNRPEERPT 490 500 FEYIQSVLDD FYTATESQYQ QQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of rat cDNA encoding hck tyrosine kinase from megakaryocytes." Okano Y., Sugimoto Y., Fukuoka M., Matsui A., Nagata K., Nozawa Y. Biochem. Biophys. Res. Commun. 181:1137-1144(1991) [PubMed: 1764064] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Megakaryocyte.
[2]	"Nucleotide sequence of a cDNA coding for rat hck tyrosine kinase and characterization of its gene product." Vijaya Gouri B.S., Rema V., Kamatkar S., Swarup G. J. Biosci. 19:117-129(1994) Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Wistar. Tissue: Spleen.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Brown Norway. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M83666 mRNA. Translation: AAA41312.1. Different initiation. S74141 mRNA. Translation: AAB20754.1. Different initiation. X62345 mRNA. Translation: CAA44218.1. Different initiation. BC078890 mRNA. Translation: AAH78890.2.
IPI	IPI00325226. IPI00817068.
PIR	JQ1321.
RefSeq	NP_037317.2.
UniGene	Rn.10945
3D structure databases
SMR	P50545. Positions 80-524.
ModBase	Search...
Protein-protein interaction databases
STRING	P50545.
PTM databases
PhosphoSite	P50545.
Genome annotation databases
Ensembl	ENSRNOT00000012432; ENSRNOP00000012432; ENSRNOG00000009331; Rattus norvegicus. [Genome view]
GeneID	25734.
KEGG	rno:25734.
UCSC	BC078890. rat.
Organism-specific databases
CTD	25734.
RGD	2785. Hck.
Phylogenomic databases
eggNOG	maNOG15300.
HOVERGEN	P50545.
InParanoid	P50545.
Enzyme and pathway databases
BRENDA	2.7.10.2. 248.
Gene expression databases
ArrayExpress	P50545.
Genevestigator	P50545.
GermOnline	ENSRNOG00000009331. Rattus norvegicus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001452. SH3_domain. IPR020473. SH3_region. IPR020748. Tyr_kinase_non-rcpt_Hck. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
PANTHER	PTHR23256:SF259. Tyr_kinase_non-rcpt_Hck. 1 hit. PTHR23256. Tyr_prot_kinase. 1 hit.
Pfam	PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN.
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	607867.

Entry information

Entry name

HCK_RAT

Accession

Primary (citable) accession number: P50545
Secondary accession number(s): Q64647, Q6AYV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 98 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)