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Reviewed, UniProtKB/Swiss-Prot P50544 (ACADV_MOUSE)

Last modified June 16, 2009. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
    EC=1.3.99.-
Alternative name(s):
    MVLCAD
      Short name=VLCAD
Gene names
Name: Acadvl
Synonyms: Vlcad
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length656 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accomodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons By similarity.

Catalytic activity

Acyl-CoA + ETF = 2,3-dehydroacyl-CoA + reduced ETF.

Cofactor

FAD.

Pathway

Lipid metabolism; mitochondrial fatty acid beta-oxidation.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion inner membrane.

Miscellaneous

A number of straight-chain acyl-CoA dehydrogenases of different substrate specificities are present in mammalian tissues.

Sequence similarities

Belongs to the acyl-CoA dehydrogenase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4141Mitochondrion By similarity
Chain42 – 656615Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
PRO_0000000517

Regions

Region42 – 483442Catalytic
Region484 – 51734Membrane-anchoring Probable

Sites

Active site4631Proton acceptor By similarity

Amino acid modifications

Modified residue2401N6-acetyllysine Ref.6
Modified residue2771N6-acetyllysine Ref.6
Cross-link332Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7

Experimental info

Sequence conflict339 – 3413NNG → GTR Ref.5
Sequence conflict4231C → W in AAA85185. Ref.5
Sequence conflict4271A → G in AAA85185. Ref.5
Sequence conflict4411M → I in AAA85185. Ref.5
Sequence conflict5071G → A in AAA85185. Ref.5
Sequence conflict5321R → P in AAA85185. Ref.5
Sequence conflict5671Q → K in AAA85185. Ref.5
Sequence conflict570 – 5712AD → GG Ref.5
Sequence conflict5731A → P Ref.5
Sequence conflict5931G → A in AAA85185. Ref.5
Sequence conflict5961T → A in AAA85185. Ref.5
Sequence conflict6121A → P in AAA85185. Ref.5
Sequence conflict6281H → Q in AAC31642. Ref.4

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Sequences

Sequence LengthMass (Da)Tools
P50544-1 [UniParc].

Last modified May 30, 2000. Version 3.
Checksum: A0110CA5C6CF4F89

FASTA65670,875
        10         20         30         40         50         60 
MQSARMTPSV GRQLLRLGAR SSRSTTVLQG QPRPISAQRL YAREATQAVL DKPETLSSDA 

        70         80         90        100        110        120 
STREKPARAE SKSFAVGMFK GQLTIDQVFP YPSVLSEEQA QFLKELVGPV ARFFEEVNDP 

       130        140        150        160        170        180 
AKNDALEKVE DDTLQGLKEL GAFGLQVPSE LGGLGLSNTQ YARLAEIVGM HDLGVSVTLG 

       190        200        210        220        230        240 
AHQSIGFKGI LLYGTKAQRE KYLPRVASGQ ALAAFCLTEP SSGSDVASIR SSAIPSPCGK 

       250        260        270        280        290        300 
YYTLNGSKIW ISNGGLADIF TVFAKTPIKD AATGAVKEKI TAFVVERSFG GVTHGLPEKK 

       310        320        330        340        350        360 
MGIKASNTSE VYFDGVKVPS ENVLGEVGDG FKVAVNILNN GRFGMAATLA GTMKSLIAKA 

       370        380        390        400        410        420 
VDHATNRTQF GDKIHNFGVI QEKLARMAIL QYVTESMAYM LSANMDQGFK DFQIEAAISK 

       430        440        450        460        470        480 
IFCSEAAWKV ADECIQIMGG MGFMKEPGVE RVLRDIRIFR IFEGANDILR LFVALQGCMD 

       490        500        510        520        530        540 
KGKELTGLGN ALKNPFGNVG LLMGEAGKQL RRRTGIGSGL SLSGIVHPEL SRSGELAVQA 

       550        560        570        580        590        600 
LDQFATVVEA KLVKHKKGIV NEQFLLQRLA DGAIDLYAMV VVLSRASRSL SEGYPTAQHE 

       610        620        630        640        650 
KMLCDSWCIE AATRIRENMA SLQSSPQHQE LFRNFRSISK AMVENGGLVT GNPLGI

« Hide

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References

« Hide 'large scale' references
[1]"Mouse very-long-chain acyl-CoA dehydrogenase (VLCAD) gene."
Andresen B.S., Lund H., Bross P., Gregersen N.
Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 129/SvJ.
Tissue: Blood.
[2]"Cloning and characterization of mouse very-long-chain acyl-CoA dehydrogenase."
Andresen B., Lund H., Bross P., Corydon M., Gregersen N.
Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: BALB/c.
Tissue: Heart.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary gland.
[4]"Chromosomal locations of the mouse fatty acid oxidation genes Cpt1a, Cpt1b, Cpt2, Acadvl, and metabolically related Crat gene."
Cox K.B., Johnson K.R., Wood P.A.
Mamm. Genome 9:608-610(1998) [PubMed: 9680378] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 84-656.
Strain: ICR.
Tissue: Liver.
[5]Rao G., Krimer D., Krasikov T., Austin C., Skoultchi A.I.
Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE OF 339-656.
[6]"Substrate and functional diversity of lysine acetylation revealed by a proteomics survey."
Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y.
Mol. Cell 23:607-618(2006) [PubMed: 16916647] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-240 AND LYS-277, MASS SPECTROMETRY.
Tissue: Liver.
[7]"A proteomics approach to identify the ubiquitinated proteins in mouse heart."
Jeon H.B., Choi E.S., Yoon J.H., Hwang J.H., Chang J.W., Lee E.K., Choi H.W., Park Z.-Y., Yoo Y.J.
Biochem. Biophys. Res. Commun. 357:731-736(2007) [PubMed: 17451654] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-332, MASS SPECTROMETRY.
Tissue: Heart.
+Additional computationally mapped references.

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Cross-references

Sequence databases

Y11770 Genomic DNA. Translation: CAA72435.1.
Z71189 mRNA. Translation: CAA94919.2.
BC026559 mRNA. Translation: AAH26559.1.
AF017176 mRNA. Translation: AAC31642.1.
U41497 mRNA. Translation: AAA85185.1.
IPIIPI00119203.
RefSeqNP_059062.1.
UniGeneMm.18630

3D structure databases

HSSPHSSP built from PDB template 1BUC based on UniProtKB Q06319.
SMRP50544. Positions 70-655.
ModBaseSearch...

PTM databases

PhosphoSiteP50544.

2-D gel databases

SWISS-2DPAGEP50544.

Proteomic databases

PRIDEP50544.

Genome annotation databases

EnsemblENSMUSG00000018574. Mus musculus. [Contig view]
GeneID11370.
KEGGmmu:11370.

Organism-specific databases

MGIMGI:895149. Acadvl.

Phylogenomic databases

HOGENOMP50544.
HOVERGENP50544.
OMAP50544. KNDALEM.

Gene expression databases

BgeeP50544.
CleanExMM_ACADVL.
GermOnlineENSMUSG00000018574. Mus musculus.

Family and domain databases

InterProIPR006089. Acyl-CoA_DH_CS.
IPR006092. Acyl-CoA_DH_N.
IPR006090. Acyl-CoA_Oxase/DH_1.
IPR006091. Acyl-CoA_Oxase/DH_M.
IPR013786. AcylCoA_DH/ox_N.
IPR013764. AcylCoA_oxidase/DH_1/2_C.
[Graphical view]
Gene3DG3DSA:2.40.110.10. Acyl_CoA_DH/ox_M. 1 hit.
G3DSA:1.10.540.10. AcylCoA_DH/ox_N. 1 hit.
G3DSA:1.20.140.10. AcylCoA_DH_1/2_C. 1 hit.
PfamPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
PROSITEPS00072. ACYL_COA_DH_1. 1 hit.
PS00073. ACYL_COA_DH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio278646.
SOURCESearch...

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Entry information

Entry nameACADV_MOUSE
AccessionPrimary (citable) accession number: P50544
Secondary accession number(s): O35289, O55133
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: June 16, 2009
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents