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P50540 (MXI1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Max-interacting protein 1

Short name=Max interactor 1
Gene names
Name:Mxi1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length228 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX. Isoform Short, which lacks a segment, has a much stronger suppressive potential and associates with a SIN3 homologous protein.

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SMC3. Interacts with RNF17. Ref.6 Ref.7

Subcellular location

Nucleus.

Developmental stage

Primarily expressed in differentiating cells. Also expressed in the proliferating cells of the developing CNS and the epidermis. In the spinal cord at embryonic day 10.5, expressed in the ventricular zone of the neural tube. Expressed at highest levels in cells accumulating in the intermediate zone. At 11.5 and 12.5 dpc, highly expressed in the intermediate zone and at reduced levels in the ventricular zone that mostly persists in the dorsal part of the neural tube. At 14.5 dpc, expressed throughout the spinal cord. In the developing epidermis at 14.5 dpc, found in the dorsal lateral epidermis. At 17 dpc, expression is restricted not only to the differentiating cells of the epidermis but also to the proliferating cell compartment and expression extends into the first differentiating cell layers, but decreases in the uppermost layers of the epidermis. Ref.4

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   Molecular functionRepressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnegative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 15467743. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay PubMed 15467743. Source: MGI

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 8649810. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P50540-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P50540-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 228228Max-interacting protein 1
PRO_0000127286

Regions

Domain67 – 11953bHLH

Natural variations

Alternative sequence1 – 3636Missing in isoform Short.
VSP_002141

Experimental info

Sequence conflict1 – 2525MERVR…RRERE → MEKHINTFLQNVQILLEAAS YLEQIEKENKK Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 7B5D4ACAEF97C4F1

FASTA22825,978
        10         20         30         40         50         60 
MERVRMINVQ RLLEAAEFLE RRERECEHGY ASSFPSMPSP RLQHSKPPRR LSRAQKHSSG 

        70         80         90        100        110        120 
SSNTSTANRS THNELEKNRR AHLRLCLERL KVLIPLGPDC TRHTTLGLLN KAKAHIKKLE 

       130        140        150        160        170        180 
EAERKSQHQL ENLEREQRFL KRRLEQLQGP QEMERIRMDS IGSTISSDRS DSEREEIEVD 

       190        200        210        220 
VESTEFSHGE ADSVSTTSIS DLDDHSSLQS VGSDEGYSSA SVKLSFAS 

« Hide

Isoform Short [UniParc].

Checksum: 23D10742E4F323CF
Show »

FASTA19221,655

References

[1]"An amino-terminal domain of Mxi1 mediates anti-Myc oncogenic activity and interacts with a homolog of the yeast transcriptional repressor SIN3."
Schreiber-Agus N., Chin L., Chen K., Torres R., Rao G., Guida P., Skoultchi A.I., DePinho R.A.
Cell 80:777-786(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
[2]"Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites."
Zervos A.S., Gyuris J., Brent R.
Cell 79:388-388(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
[3]"Cloning and sequencing of the murine Mxi1 cDNA."
Shimizu E., Shirasawa H., Kodama K., Koseki K., Sato T., Simizu B.
Gene 152:283-284(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C3H.
[4]"Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation."
Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N.
EMBO J. 14:5646-5659(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[5]Erratum
Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N.
EMBO J. 15:2030-2030(1996) [PubMed] [Europe PMC] [Abstract]
[6]"Mmip1: a novel leucine zipper protein that reverses the suppressive effects of mad family members on C-myc."
Gupta K., Anand G., Yin X.Y., Prochownik E.V.
Oncogene 16:1149-1159(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SMC3.
[7]"Mmip-2, a novel RING finger protein that interacts with mad members of the Myc oncoprotein network."
Yin X.-Y., Gupta K., Prochownik E.V.
Oncogene 18:6621-6634(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RNF17.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L38822 mRNA. Translation: AAA65684.1.
L38821 mRNA. Translation: AAA65685.1.
U24674 mRNA. Translation: AAA65183.1.
U24673 mRNA. Translation: AAA65182.1.
D31824 mRNA. Translation: BAA06612.1.
CCDSCCDS29901.1. [P50540-1]
CCDS38023.1. [P50540-2]
PIRA56069.
JC4050.
RefSeqNP_001008543.1. NM_001008543.2. [P50540-2]
NP_034977.1. NM_010847.3. [P50540-1]
XP_006526801.1. XM_006526738.1. [P50540-2]
XP_006526802.1. XM_006526739.1. [P50540-2]
XP_006526803.1. XM_006526740.1. [P50540-2]
XP_006526804.1. XM_006526741.1. [P50540-2]
UniGeneMm.2154.

3D structure databases

ProteinModelPortalP50540.
SMRP50540. Positions 68-144.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201630. 5 interactions.
DIPDIP-489N.
IntActP50540. 1 interaction.

PTM databases

PhosphoSiteP50540.

Proteomic databases

PRIDEP50540.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025998; ENSMUSP00000025998; ENSMUSG00000025025. [P50540-1]
ENSMUST00000111737; ENSMUSP00000107366; ENSMUSG00000025025. [P50540-2]
GeneID17859.
KEGGmmu:17859.
UCSCuc008hwq.1. mouse. [P50540-1]

Organism-specific databases

CTD4601.
MGIMGI:97245. Mxi1.

Phylogenomic databases

eggNOGNOG238408.
GeneTreeENSGT00510000046360.
HOGENOMHOG000247060.
HOVERGENHBG006314.
KOK09114.
OMAGLQDPKP.
PhylomeDBP50540.

Gene expression databases

ArrayExpressP50540.
BgeeP50540.
CleanExMM_MXI1.
GenevestigatorP50540.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
[Graphical view]
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMXI1. mouse.
NextBio292617.
PROP50540.
SOURCESearch...

Entry information

Entry nameMXI1_MOUSE
AccessionPrimary (citable) accession number: P50540
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot