Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P50540

- MXI1_MOUSE

UniProt

P50540 - MXI1_MOUSE

Protein

Max-interacting protein 1

Gene

Mxi1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX. Isoform Short, which lacks a segment, has a much stronger suppressive potential and associates with a SIN3 homologous protein.

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Max-interacting protein 1
    Short name:
    Max interactor 1
    Gene namesi
    Name:Mxi1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:97245. Mxi1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 228228Max-interacting protein 1PRO_0000127286Add
    BLAST

    Proteomic databases

    PRIDEiP50540.

    PTM databases

    PhosphoSiteiP50540.

    Expressioni

    Developmental stagei

    Primarily expressed in differentiating cells. Also expressed in the proliferating cells of the developing CNS and the epidermis. In the spinal cord at embryonic day 10.5, expressed in the ventricular zone of the neural tube. Expressed at highest levels in cells accumulating in the intermediate zone. At 11.5 and 12.5 dpc, highly expressed in the intermediate zone and at reduced levels in the ventricular zone that mostly persists in the dorsal part of the neural tube. At 14.5 dpc, expressed throughout the spinal cord. In the developing epidermis at 14.5 dpc, found in the dorsal lateral epidermis. At 17 dpc, expression is restricted not only to the differentiating cells of the epidermis but also to the proliferating cell compartment and expression extends into the first differentiating cell layers, but decreases in the uppermost layers of the epidermis.1 Publication

    Gene expression databases

    ArrayExpressiP50540.
    BgeeiP50540.
    CleanExiMM_MXI1.
    GenevestigatoriP50540.

    Interactioni

    Subunit structurei

    Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SMC3. Interacts with RNF17.2 Publications

    Protein-protein interaction databases

    BioGridi201630. 5 interactions.
    DIPiDIP-489N.
    IntActiP50540. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP50540.
    SMRiP50540. Positions 68-144.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini67 – 11953bHLHPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG238408.
    GeneTreeiENSGT00510000046360.
    HOGENOMiHOG000247060.
    HOVERGENiHBG006314.
    KOiK09114.
    OMAiGLQDPKP.
    PhylomeDBiP50540.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P50540-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MERVRMINVQ RLLEAAEFLE RRERECEHGY ASSFPSMPSP RLQHSKPPRR    50
    LSRAQKHSSG SSNTSTANRS THNELEKNRR AHLRLCLERL KVLIPLGPDC 100
    TRHTTLGLLN KAKAHIKKLE EAERKSQHQL ENLEREQRFL KRRLEQLQGP 150
    QEMERIRMDS IGSTISSDRS DSEREEIEVD VESTEFSHGE ADSVSTTSIS 200
    DLDDHSSLQS VGSDEGYSSA SVKLSFAS 228
    Length:228
    Mass (Da):25,978
    Last modified:October 1, 1996 - v1
    Checksum:i7B5D4ACAEF97C4F1
    GO
    Isoform Short (identifier: P50540-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-36: Missing.

    Show »
    Length:192
    Mass (Da):21,655
    Checksum:i23D10742E4F323CF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 2525MERVR…RRERE → MEKHINTFLQNVQILLEAAS YLEQIEKENKK(PubMed:7835718)CuratedAdd
    BLAST

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 3636Missing in isoform Short. 2 PublicationsVSP_002141Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38822 mRNA. Translation: AAA65684.1.
    L38821 mRNA. Translation: AAA65685.1.
    U24674 mRNA. Translation: AAA65183.1.
    U24673 mRNA. Translation: AAA65182.1.
    D31824 mRNA. Translation: BAA06612.1.
    CCDSiCCDS29901.1. [P50540-1]
    CCDS38023.1. [P50540-2]
    PIRiA56069.
    JC4050.
    RefSeqiNP_001008543.1. NM_001008543.2. [P50540-2]
    NP_034977.1. NM_010847.3. [P50540-1]
    XP_006526801.1. XM_006526738.1. [P50540-2]
    XP_006526802.1. XM_006526739.1. [P50540-2]
    XP_006526803.1. XM_006526740.1. [P50540-2]
    XP_006526804.1. XM_006526741.1. [P50540-2]
    UniGeneiMm.2154.

    Genome annotation databases

    EnsembliENSMUST00000025998; ENSMUSP00000025998; ENSMUSG00000025025. [P50540-1]
    ENSMUST00000111737; ENSMUSP00000107366; ENSMUSG00000025025. [P50540-2]
    GeneIDi17859.
    KEGGimmu:17859.
    UCSCiuc008hwq.1. mouse. [P50540-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38822 mRNA. Translation: AAA65684.1 .
    L38821 mRNA. Translation: AAA65685.1 .
    U24674 mRNA. Translation: AAA65183.1 .
    U24673 mRNA. Translation: AAA65182.1 .
    D31824 mRNA. Translation: BAA06612.1 .
    CCDSi CCDS29901.1. [P50540-1 ]
    CCDS38023.1. [P50540-2 ]
    PIRi A56069.
    JC4050.
    RefSeqi NP_001008543.1. NM_001008543.2. [P50540-2 ]
    NP_034977.1. NM_010847.3. [P50540-1 ]
    XP_006526801.1. XM_006526738.1. [P50540-2 ]
    XP_006526802.1. XM_006526739.1. [P50540-2 ]
    XP_006526803.1. XM_006526740.1. [P50540-2 ]
    XP_006526804.1. XM_006526741.1. [P50540-2 ]
    UniGenei Mm.2154.

    3D structure databases

    ProteinModelPortali P50540.
    SMRi P50540. Positions 68-144.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201630. 5 interactions.
    DIPi DIP-489N.
    IntActi P50540. 1 interaction.

    PTM databases

    PhosphoSitei P50540.

    Proteomic databases

    PRIDEi P50540.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025998 ; ENSMUSP00000025998 ; ENSMUSG00000025025 . [P50540-1 ]
    ENSMUST00000111737 ; ENSMUSP00000107366 ; ENSMUSG00000025025 . [P50540-2 ]
    GeneIDi 17859.
    KEGGi mmu:17859.
    UCSCi uc008hwq.1. mouse. [P50540-1 ]

    Organism-specific databases

    CTDi 4601.
    MGIi MGI:97245. Mxi1.

    Phylogenomic databases

    eggNOGi NOG238408.
    GeneTreei ENSGT00510000046360.
    HOGENOMi HOG000247060.
    HOVERGENi HBG006314.
    KOi K09114.
    OMAi GLQDPKP.
    PhylomeDBi P50540.

    Miscellaneous databases

    ChiTaRSi MXI1. mouse.
    NextBioi 292617.
    PROi P50540.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P50540.
    Bgeei P50540.
    CleanExi MM_MXI1.
    Genevestigatori P50540.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An amino-terminal domain of Mxi1 mediates anti-Myc oncogenic activity and interacts with a homolog of the yeast transcriptional repressor SIN3."
      Schreiber-Agus N., Chin L., Chen K., Torres R., Rao G., Guida P., Skoultchi A.I., DePinho R.A.
      Cell 80:777-786(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    2. "Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites."
      Zervos A.S., Gyuris J., Brent R.
      Cell 79:388-388(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    3. "Cloning and sequencing of the murine Mxi1 cDNA."
      Shimizu E., Shirasawa H., Kodama K., Koseki K., Sato T., Simizu B.
      Gene 152:283-284(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H.
    4. "Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation."
      Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N.
      EMBO J. 14:5646-5659(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    5. "Mmip1: a novel leucine zipper protein that reverses the suppressive effects of mad family members on C-myc."
      Gupta K., Anand G., Yin X.Y., Prochownik E.V.
      Oncogene 16:1149-1159(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SMC3.
    6. "Mmip-2, a novel RING finger protein that interacts with mad members of the Myc oncoprotein network."
      Yin X.-Y., Gupta K., Prochownik E.V.
      Oncogene 18:6621-6634(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RNF17.

    Entry informationi

    Entry nameiMXI1_MOUSE
    AccessioniPrimary (citable) accession number: P50540
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3