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P50540

- MXI1_MOUSE

UniProt

P50540 - MXI1_MOUSE

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Protein

Max-interacting protein 1

Gene

Mxi1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX. Isoform Short, which lacks a segment, has a much stronger suppressive potential and associates with a SIN3 homologous protein.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Max-interacting protein 1
Short name:
Max interactor 1
Gene namesi
Name:Mxi1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:97245. Mxi1.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 228228Max-interacting protein 1PRO_0000127286Add
BLAST

Proteomic databases

PRIDEiP50540.

PTM databases

PhosphoSiteiP50540.

Expressioni

Developmental stagei

Primarily expressed in differentiating cells. Also expressed in the proliferating cells of the developing CNS and the epidermis. In the spinal cord at embryonic day 10.5, expressed in the ventricular zone of the neural tube. Expressed at highest levels in cells accumulating in the intermediate zone. At 11.5 and 12.5 dpc, highly expressed in the intermediate zone and at reduced levels in the ventricular zone that mostly persists in the dorsal part of the neural tube. At 14.5 dpc, expressed throughout the spinal cord. In the developing epidermis at 14.5 dpc, found in the dorsal lateral epidermis. At 17 dpc, expression is restricted not only to the differentiating cells of the epidermis but also to the proliferating cell compartment and expression extends into the first differentiating cell layers, but decreases in the uppermost layers of the epidermis.1 Publication

Gene expression databases

BgeeiP50540.
CleanExiMM_MXI1.
ExpressionAtlasiP50540. baseline and differential.
GenevestigatoriP50540.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SMC3. Interacts with RNF17.2 Publications

Protein-protein interaction databases

BioGridi201630. 5 interactions.
DIPiDIP-489N.
IntActiP50540. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP50540.
SMRiP50540. Positions 68-144.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini67 – 11953bHLHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG238408.
GeneTreeiENSGT00510000046360.
HOGENOMiHOG000247060.
HOVERGENiHBG006314.
InParanoidiP50540.
KOiK09114.
OMAiGLQDPKP.
PhylomeDBiP50540.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P50540-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERVRMINVQ RLLEAAEFLE RRERECEHGY ASSFPSMPSP RLQHSKPPRR
60 70 80 90 100
LSRAQKHSSG SSNTSTANRS THNELEKNRR AHLRLCLERL KVLIPLGPDC
110 120 130 140 150
TRHTTLGLLN KAKAHIKKLE EAERKSQHQL ENLEREQRFL KRRLEQLQGP
160 170 180 190 200
QEMERIRMDS IGSTISSDRS DSEREEIEVD VESTEFSHGE ADSVSTTSIS
210 220
DLDDHSSLQS VGSDEGYSSA SVKLSFAS
Length:228
Mass (Da):25,978
Last modified:October 1, 1996 - v1
Checksum:i7B5D4ACAEF97C4F1
GO
Isoform Short (identifier: P50540-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.

Show »
Length:192
Mass (Da):21,655
Checksum:i23D10742E4F323CF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 2525MERVR…RRERE → MEKHINTFLQNVQILLEAAS YLEQIEKENKK(PubMed:7835718)CuratedAdd
BLAST

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3636Missing in isoform Short. 2 PublicationsVSP_002141Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L38822 mRNA. Translation: AAA65684.1.
L38821 mRNA. Translation: AAA65685.1.
U24674 mRNA. Translation: AAA65183.1.
U24673 mRNA. Translation: AAA65182.1.
D31824 mRNA. Translation: BAA06612.1.
CCDSiCCDS29901.1. [P50540-1]
CCDS38023.1. [P50540-2]
PIRiA56069.
JC4050.
RefSeqiNP_001008543.1. NM_001008543.2. [P50540-2]
NP_034977.1. NM_010847.3. [P50540-1]
XP_006526801.1. XM_006526738.1. [P50540-2]
XP_006526802.1. XM_006526739.1. [P50540-2]
XP_006526803.1. XM_006526740.1. [P50540-2]
XP_006526804.1. XM_006526741.1. [P50540-2]
UniGeneiMm.2154.

Genome annotation databases

EnsembliENSMUST00000025998; ENSMUSP00000025998; ENSMUSG00000025025. [P50540-1]
ENSMUST00000111737; ENSMUSP00000107366; ENSMUSG00000025025. [P50540-2]
GeneIDi17859.
KEGGimmu:17859.
UCSCiuc008hwq.1. mouse. [P50540-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L38822 mRNA. Translation: AAA65684.1 .
L38821 mRNA. Translation: AAA65685.1 .
U24674 mRNA. Translation: AAA65183.1 .
U24673 mRNA. Translation: AAA65182.1 .
D31824 mRNA. Translation: BAA06612.1 .
CCDSi CCDS29901.1. [P50540-1 ]
CCDS38023.1. [P50540-2 ]
PIRi A56069.
JC4050.
RefSeqi NP_001008543.1. NM_001008543.2. [P50540-2 ]
NP_034977.1. NM_010847.3. [P50540-1 ]
XP_006526801.1. XM_006526738.1. [P50540-2 ]
XP_006526802.1. XM_006526739.1. [P50540-2 ]
XP_006526803.1. XM_006526740.1. [P50540-2 ]
XP_006526804.1. XM_006526741.1. [P50540-2 ]
UniGenei Mm.2154.

3D structure databases

ProteinModelPortali P50540.
SMRi P50540. Positions 68-144.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201630. 5 interactions.
DIPi DIP-489N.
IntActi P50540. 1 interaction.

PTM databases

PhosphoSitei P50540.

Proteomic databases

PRIDEi P50540.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025998 ; ENSMUSP00000025998 ; ENSMUSG00000025025 . [P50540-1 ]
ENSMUST00000111737 ; ENSMUSP00000107366 ; ENSMUSG00000025025 . [P50540-2 ]
GeneIDi 17859.
KEGGi mmu:17859.
UCSCi uc008hwq.1. mouse. [P50540-1 ]

Organism-specific databases

CTDi 4601.
MGIi MGI:97245. Mxi1.

Phylogenomic databases

eggNOGi NOG238408.
GeneTreei ENSGT00510000046360.
HOGENOMi HOG000247060.
HOVERGENi HBG006314.
InParanoidi P50540.
KOi K09114.
OMAi GLQDPKP.
PhylomeDBi P50540.

Miscellaneous databases

ChiTaRSi MXI1. mouse.
NextBioi 292617.
PROi P50540.
SOURCEi Search...

Gene expression databases

Bgeei P50540.
CleanExi MM_MXI1.
ExpressionAtlasi P50540. baseline and differential.
Genevestigatori P50540.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
[Graphical view ]
Pfami PF00010. HLH. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "An amino-terminal domain of Mxi1 mediates anti-Myc oncogenic activity and interacts with a homolog of the yeast transcriptional repressor SIN3."
    Schreiber-Agus N., Chin L., Chen K., Torres R., Rao G., Guida P., Skoultchi A.I., DePinho R.A.
    Cell 80:777-786(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
  2. "Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites."
    Zervos A.S., Gyuris J., Brent R.
    Cell 79:388-388(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
  3. "Cloning and sequencing of the murine Mxi1 cDNA."
    Shimizu E., Shirasawa H., Kodama K., Koseki K., Sato T., Simizu B.
    Gene 152:283-284(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H.
  4. "Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation."
    Hurlin P.J., Queva C., Koskinen P.J., Steingrimsson E., Ayer D.E., Copeland N.G., Jenkins N.A., Eisenman R.N.
    EMBO J. 14:5646-5659(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "Mmip1: a novel leucine zipper protein that reverses the suppressive effects of mad family members on C-myc."
    Gupta K., Anand G., Yin X.Y., Prochownik E.V.
    Oncogene 16:1149-1159(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SMC3.
  6. "Mmip-2, a novel RING finger protein that interacts with mad members of the Myc oncoprotein network."
    Yin X.-Y., Gupta K., Prochownik E.V.
    Oncogene 18:6621-6634(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RNF17.

Entry informationi

Entry nameiMXI1_MOUSE
AccessioniPrimary (citable) accession number: P50540
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3