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Protein

Max-interacting protein 1

Gene

Mxi1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX. Isoform Short, which lacks a segment, has a much stronger suppressive potential and associates with a SIN3 homologous protein.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Max-interacting protein 1
Short name:
Max interactor 1
Gene namesi
Name:Mxi1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:97245 Mxi1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001272861 – 228Max-interacting protein 1Add BLAST228

Proteomic databases

PaxDbiP50540
PRIDEiP50540

PTM databases

PhosphoSitePlusiP50540

Expressioni

Developmental stagei

Primarily expressed in differentiating cells. Also expressed in the proliferating cells of the developing CNS and the epidermis. In the spinal cord at embryonic day 10.5, expressed in the ventricular zone of the neural tube. Expressed at highest levels in cells accumulating in the intermediate zone. At 11.5 and 12.5 dpc, highly expressed in the intermediate zone and at reduced levels in the ventricular zone that mostly persists in the dorsal part of the neural tube. At 14.5 dpc, expressed throughout the spinal cord. In the developing epidermis at 14.5 dpc, found in the dorsal lateral epidermis. At 17 dpc, expression is restricted not only to the differentiating cells of the epidermis but also to the proliferating cell compartment and expression extends into the first differentiating cell layers, but decreases in the uppermost layers of the epidermis.1 Publication

Gene expression databases

BgeeiENSMUSG00000025025
CleanExiMM_MXI1
ExpressionAtlasiP50540 baseline and differential
GenevisibleiP50540 MM

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SMC3. Interacts with RNF17.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201630, 5 interactors
DIPiDIP-489N
IntActiP50540, 1 interactor
STRINGi10090.ENSMUSP00000003870

Structurei

3D structure databases

ProteinModelPortaliP50540
SMRiP50540
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini67 – 119bHLHPROSITE-ProRule annotationAdd BLAST53

Phylogenomic databases

eggNOGiENOG410IPD5 Eukaryota
ENOG4111MIN LUCA
GeneTreeiENSGT00510000046360
HOGENOMiHOG000247060
HOVERGENiHBG006314
InParanoidiP50540
KOiK09114
OMAiGLQDPKP
PhylomeDBiP50540

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P50540-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERVRMINVQ RLLEAAEFLE RRERECEHGY ASSFPSMPSP RLQHSKPPRR
60 70 80 90 100
LSRAQKHSSG SSNTSTANRS THNELEKNRR AHLRLCLERL KVLIPLGPDC
110 120 130 140 150
TRHTTLGLLN KAKAHIKKLE EAERKSQHQL ENLEREQRFL KRRLEQLQGP
160 170 180 190 200
QEMERIRMDS IGSTISSDRS DSEREEIEVD VESTEFSHGE ADSVSTTSIS
210 220
DLDDHSSLQS VGSDEGYSSA SVKLSFAS
Length:228
Mass (Da):25,978
Last modified:October 1, 1996 - v1
Checksum:i7B5D4ACAEF97C4F1
GO
Isoform Short (identifier: P50540-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.

Show »
Length:192
Mass (Da):21,655
Checksum:i23D10742E4F323CF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 25MERVR…RRERE → MEKHINTFLQNVQILLEAAS YLEQIEKENKK (PubMed:7835718).CuratedAdd BLAST25

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0021411 – 36Missing in isoform Short. 2 PublicationsAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38822 mRNA Translation: AAA65684.1
L38821 mRNA Translation: AAA65685.1
U24674 mRNA Translation: AAA65183.1
U24673 mRNA Translation: AAA65182.1
D31824 mRNA Translation: BAA06612.1
CCDSiCCDS29901.1 [P50540-1]
CCDS38023.1 [P50540-2]
PIRiA56069
JC4050
RefSeqiNP_001008543.1, NM_001008543.2 [P50540-2]
NP_034977.1, NM_010847.3 [P50540-1]
XP_006526801.1, XM_006526738.1 [P50540-2]
XP_006526802.1, XM_006526739.1 [P50540-2]
XP_006526803.1, XM_006526740.1 [P50540-2]
UniGeneiMm.2154

Genome annotation databases

EnsembliENSMUST00000025998; ENSMUSP00000025998; ENSMUSG00000025025 [P50540-1]
ENSMUST00000111737; ENSMUSP00000107366; ENSMUSG00000025025 [P50540-2]
GeneIDi17859
KEGGimmu:17859
UCSCiuc008hwq.1 mouse [P50540-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMXI1_MOUSE
AccessioniPrimary (citable) accession number: P50540
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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