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Protein

Max dimerization protein 1

Gene

Mxd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor. MAD binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MAD thus antagonizes MYC transcriptional activity by competing for MAX.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Max dimerization protein 1
Short name:
Max dimerizer 1
Alternative name(s):
Protein MAD
Gene namesi
Name:Mxd1
Synonyms:Mad, Mad1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96908. Mxd1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001272651 – 227Max dimerization protein 1Add BLAST227

Post-translational modificationi

Ubiquitinated by BIRC2/c-IAP1, leading to its subsequent degradation by the proteasome.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiP50538.
PRIDEiP50538.

PTM databases

iPTMnetiP50538.
PhosphoSitePlusiP50538.

Expressioni

Developmental stagei

Expressed during neural and epidermal differentiation. Primarily expressed in growth-arrested differentiating cells. In the spinal cord at embryonic day 10.5, a strong expressesion seen in the differentiating cells of the intermediate zone at the ventral part of the neural tube and weakly in the ventricular zone. At 11.5 and 12.5 dpc, highly expressed in the intermediate zone and at reduced levels in the ventricular zone that mostly persists in the dorsal part of the neural tube. At 14.5 dpc, expressed throughout the spinal cord. In the developing epidermis at 14.5 dpc, found in the dorsal lateral epidermis. At 17.5 dpc expressed in the cell cycle arrested, differentiating cells of the suprabasal malphigian layer.1 Publication

Gene expression databases

CleanExiMM_MXD1.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with RNF17.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

CORUMiP50538.
DIPiDIP-864N.
STRINGi10090.ENSMUSP00000001184.

Structurei

3D structure databases

ProteinModelPortaliP50538.
SMRiP50538.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 107bHLHPROSITE-ProRule annotationAdd BLAST53

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi21 – 48Nuclear localization signalSequence analysisAdd BLAST28

Phylogenomic databases

eggNOGiENOG410IPD6. Eukaryota.
ENOG4111X99. LUCA.
HOGENOMiHOG000247060.
HOVERGENiHBG006314.
InParanoidiP50538.
PhylomeDBiP50538.

Family and domain databases

CDDicd00083. HLH. 1 hit.
Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR011598. bHLH_dom.
IPR036638. HLH_DNA-bd_sf.
PfamiView protein in Pfam
PF00010. HLH. 1 hit.
SMARTiView protein in SMART
SM00353. HLH. 1 hit.
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.

Sequencei

Sequence statusi: Complete.

P50538-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAVGMNIQ LLLEAADYLE RREREAEHGY ASMLPYSKDR DAFKRRNKPK
60 70 80 90 100
KNSTSSRSTH NEMEKNRRAH LRLCLEKLKG LVPLGPESSR HTTLSLLTKA
110 120 130 140 150
KLHIKKLEDC DRKAVHQIDQ LQREQRHLKR RLEKLGAERT RMDSVGSVVS
160 170 180 190 200
SERSDSDREE LDVDVDVDVD VDVEGTDYLN GDLGWSSSVS DSDERGSMQS
210 220
LGSDEGYSSA TVKRAKLQDG HKAGLGL
Length:227
Mass (Da):25,562
Last modified:November 1, 1997 - v2
Checksum:i92736F1C72E419FC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14E → A in AAB00682 (PubMed:7667316).Curated1
Sequence conflicti160Missing in CAA58168 (PubMed:7896882).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38926 mRNA. Translation: AAB00682.1.
U20614 mRNA. Translation: AAA62310.1.
X83106 mRNA. Translation: CAA58168.1.
CCDSiCCDS51835.1.
PIRiA57074. S51642.
UniGeneiMm.279580.

Similar proteinsi

Entry informationi

Entry nameiMAD1_MOUSE
AccessioniPrimary (citable) accession number: P50538
Secondary accession number(s): Q60798, Q61825
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: October 25, 2017
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot