Reviewed, UniProtKB/Swiss-Prot P50535 (MSL1_DROME)

Names and origin

Protein names

Recommended name:
    Protein male-specific lethal-1

Gene names

Name:	msl-1
ORF Names:	CG10385

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1039 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The MSL proteins are essential for elevating transcription of the single X chromosome in the male (X chromosome dosage compensation). Msl-1 is a pioneer protein. Mle, msl-1 and msl-3 are colocalized on the X chromosome. Each of the MSL proteins requires all the other MSLs for wild-type X-chromosome binding.
Subunit structure	Seems to form a tight complex with msl-2. Interacts with tamo via the nuclear localization signal. Ref.6
Subcellular location	Nucleus. Note: Msl-1 is associated with hundreds of discrete sites along the length of the X chromosome in males and not in females, and is also associated with 10-20 autosomal sites in males.
Sequence similarities	Belongs to the msl-1 family.

Ontologies

Keywords
Cellular component	Nucleus
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dosage compensation complex assembly during dosage compensation by hyperactivation of X chromosome Traceable author statement. Source: FlyBase
Cellular component	X chromosome located dosage compensation complex, transcription activating Traceable author statement. Source: FlyBase
Molecular function	DNA binding Inferred from direct assay. Source: FlyBase chromatin binding Inferred from direct assay. Source: FlyBase protein binding Ref.6 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
tamo	Q9W1A4	1	EBI-155450,EBI-91385

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1039	1039	Protein male-specific lethal-1		PRO_0000096597
Regions
	Motif	1032 – 1037	6	Nuclear localization signal
Experimental info
	Sequence conflict	26	1	R → G in AAA98918. Ref.1
	Sequence conflict	492	1	S → L in AAA98918. Ref.1
	Sequence conflict	670	1	M → I in AAA98918. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P50535-1 [UniParc]. Last modified July 30, 2004. Version 2. Checksum: 644C21C0390A2750	FASTA	1,039	117,503
	10 20 30 40 50 60 MDKRFKWPPK KRANYLESPY PHIPSRGRQR NLHGHPNQTQ HLHQHPGKIY ERQQYGNGRG 70 80 90 100 110 120 GHGGGNNNYR KLLHSLPAEH GGGAMAPPSS GGTVCAGADM VKLISENNNL RRMVMLNLNL 130 140 150 160 170 180 MQEQTDSIAA KDKELDDQSA KMSVVKAQNE ELKQAVAQLE AANQELCKQL RRKNQRRNDN 190 200 210 220 230 240 DDDDDDPPLP PAAPQQKLIR CHAETQTVFR EREQGTQTID AQPQFANALP RGINMKESPA 250 260 270 280 290 300 LDHHAGAVTN QPASKRSESK GRGEFNGKKV STFILQRMNQ DFEHHIHEQT EVAEEHEMEA 310 320 330 340 350 360 HKEQISQEED QLVAEEDHLH MQEVHTEEVV GGDIFHDALE SIEMEVVTEE LVDMEEHGQS 370 380 390 400 410 420 VDANGHIEED DDEDDEDDEN SDKDDDSEED DYPWMHSDAD VNARTEEELW QNQNYLLELD 430 440 450 460 470 480 PTEEKTCAPS AHSTPNHQQK SSTQAEIRKE GNQNRITEKL LQLKPEPMVD ALEAPILPKW 490 500 510 520 530 540 VAFKKKDKEH ESVPESPEVP KQQPHQEDAI VDHNAIKNQL EVPKPDLKPK DQPKDEQRQD 550 560 570 580 590 600 GQLDVRVEPQ EDVRKVQKET LKRQPEDAPK HLPKAVAPKV TKTSSRESTL PKANTADIKD 610 620 630 640 650 660 APAQKVIANH QSTKTQTDPV KTQRLQVKIR QYEMHPDMRT GSSAPSDIRK QKNVDPVSTP 670 680 690 700 710 720 ETKTIKSKSM LVNDKKTTSE TSQSPDQEID VETVRRKLAE HLKKELLSQS HSSQVTLKKI 730 740 750 760 770 780 RERVATNLIY PPPSAPVSST TITPAPTPST TPTPGSTPQH AVTSSMDQEI SAAKSKSKAA 790 800 810 820 830 840 EQIATPLTPQ SNSSVSSTTS TIRKTLNNCS PHTYSKATAR SGKLQSRFRT ATFPYSTRTW 850 860 870 880 890 900 EDQEFHCDNE FFLEEADELL ADNPSLEIPK WRDVPVPPSS DKIDTELLSD ATFERRHQKY 910 920 930 940 950 960 VKDEVDRKCR DARYMKEQIR LEQLRMRRNQ DEVLVALDPL RASTFYPLPE DIEAIQFVNE 970 980 990 1000 1010 1020 VTVQAFGENV VNMEARDDFG VPWVDAIEAP TSIARSKALA EPVATLASKK IPTTAAEARH 1030 QENHSSYVFP KRRKRQKNR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of msl-2 causes assembly of dosage compensation regulators on the X chromosomes and female lethality in Drosophila." Kelley R.L., Solovyeva I., Lyman L.M., Richman R., Solovyev V., Kuroda M.I. Cell 81:867-877(1995) [PubMed: 7781064] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"The male-specific lethal-one (msl-1) gene of Drosophila melanogaster encodes a novel protein that associates with the X chromosome in males." Palmer M.J., Mergner V.A., Richman R., Manning J.E., Kuroda M.I., Lucchesi J.C. Genetics 134:545-557(1993) [PubMed: 8325488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 85-1039. Strain: Canton-S.
[6]	"Tamo selectively modulates nuclear import in Drosophila." Minakhina S., Yang J., Steward R. Genes Cells 8:299-310(2003) [PubMed: 12653959] [Abstract] Cited for: INTERACTION WITH TAMO.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42514 Genomic DNA. Translation: AAA98918.1. AE014134 Genomic DNA. Translation: AAF53689.1. BT023828 mRNA. Translation: AAZ86749.1.
PIR	S52959.
RefSeq	NP_476896.1.
UniGene	Dm.458
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P50535. 6 interactions.
Genome annotation databases
Ensembl	FBgn0005617. Drosophila melanogaster. [Contig view]
GeneID	35121.
KEGG	dme:Dmel_CG10385.
NMPDR	fig|7227.3.peg.2853.
Organism-specific databases
FlyBase	FBgn0005617. msl-1.
Phylogenomic databases
HOGENOM	P50535.
OMA	P50535. VQAFGEN.
Gene expression databases
ArrayExpress	P50535.
GermOnline	CG10385. Drosophila melanogaster.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	791966.

Entry information

Entry name

MSL1_DROME

Accession

Primary (citable) accession number: P50535
Secondary accession number(s): Q3ZAP1, Q9VJ66

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 30, 2004
Last modified:	June 16, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project