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Protein

E3 ubiquitin-protein ligase msl-2

Gene

msl-2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The Msl proteins are essential for elevating transcription of the single X chromosome in the male (X chromosome dosage compensation). Msl-2 is required for translation and/or stability of msl-1 in males. In complex with msl-1, acts as an E3 ubiquitin ligase that promotes ubiquitination of histone H2B.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri41 – 85RING-typePROSITE-ProRule annotationAdd BLAST45

GO - Molecular functioni

  • chromatin binding Source: FlyBase
  • DNA binding Source: FlyBase
  • ligase activity Source: UniProtKB-KW
  • transcription factor binding Source: FlyBase
  • ubiquitin protein ligase activity Source: FlyBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • dosage compensation Source: FlyBase
  • dosage compensation by hyperactivation of X chromosome Source: FlyBase
  • dosage compensation complex assembly involved in dosage compensation by hyperactivation of X chromosome Source: FlyBase
  • histone H4-K16 acetylation Source: GO_Central
  • protein autoubiquitination Source: FlyBase
  • protein ubiquitination Source: FlyBase
  • sensory perception of pain Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-3214847. HATs acetylate histones.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase msl-2 (EC:6.3.2.-)
Alternative name(s):
Protein male-specific lethal-2
Gene namesi
Name:msl-2
ORF Names:CG3241
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0005616. msl-2.

Subcellular locationi

  • Nucleus
  • Chromosome

  • Note: Msl-1 and msl-2 colocalize to many sites on the male X chromosome.

GO - Cellular componenti

  • dosage compensation complex Source: FlyBase
  • MSL complex Source: FlyBase
  • nuclear chromosome Source: FlyBase
  • X chromosome Source: FlyBase
  • X chromosome located dosage compensation complex, transcription activating Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559621 – 773E3 ubiquitin-protein ligase msl-2Add BLAST773

Proteomic databases

PaxDbiP50534.
PRIDEiP50534.

Expressioni

Developmental stagei

Only produced in males.

Gene expression databases

BgeeiFBgn0005616.
ExpressionAtlasiP50534. baseline.
GenevisibleiP50534. DM.

Interactioni

Subunit structurei

Interacts with msl-1 in a tight complex to form a dosage compensation protein complex.3 Publications

GO - Molecular functioni

  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi59776. 10 interactors.
IntActiP50534. 1 interactor.
STRINGi7227.FBpp0077259.

Structurei

Secondary structure

1773
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi530 – 533Combined sources4
Helixi535 – 537Combined sources3
Turni538 – 540Combined sources3
Helixi545 – 548Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LUANMR-A520-570[»]
4RKGX-ray2.50A/B520-570[»]
4RKHX-ray2.00C/D/E/F520-570[»]
ProteinModelPortaliP50534.
SMRiP50534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili424 – 468Sequence analysisAdd BLAST45

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi525 – 561Cys-richAdd BLAST37
Compositional biasi685 – 713Pro-richAdd BLAST29

Sequence similaritiesi

Belongs to the MSL2 family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri41 – 85RING-typePROSITE-ProRule annotationAdd BLAST45

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IGUA. Eukaryota.
ENOG410ZJAZ. LUCA.
GeneTreeiENSGT00390000016814.
InParanoidiP50534.
KOiK13164.
OMAiCEDCSDF.
OrthoDBiEOG091G0IAA.
PhylomeDBiP50534.

Family and domain databases

CDDicd13122. MSL2_CXC. 1 hit.
InterProiIPR032049. Msl2-CXC.
IPR032043. Msl2_Znf-RING.
IPR033467. Tesmin/TSO1-like_CXC.
IPR001841. Znf_RING.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF16682. MSL2-CXC. 1 hit.
PF16685. zf-RING_10. 1 hit.
[Graphical view]
SMARTiSM01114. CXC. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQTAYLKVT RIAMRSASNL SKRRVEELNS GLGELRQLLS CVVCCQLLVD
60 70 80 90 100
PYSPKGKRCQ HNVCRLCLRG KKHLFPSCTQ CEGCSDFKTY EENRMMAAQL
110 120 130 140 150
LCYKTLCVHL LHSALFGELA GMRPQVAREL VPRIKLPPKT TQEFIREGSN
160 170 180 190 200
ISDTFDIFLP QPDLPFLKDM PTSLPAETPP TSAVTTPELP YDHHLNISDI
210 220 230 240 250
EAEAAATAEQ GHFSPLPLLP TGSRMGMLSH AGQIVIATES SESGFMDQAW
260 270 280 290 300
TDQVDLSGTV SVSKYTNSGN NFAVSYVMPT SATTKFDPQE LQIGQVVQMA
310 320 330 340 350
DSTQLAVLAA VEETVETSTQ LTVLSTTVEE TVETSTQLEV LTSAEEPNEI
360 370 380 390 400
SDQLANLQVE ESDEALVEET VEEAEGTSIP SEVVAEHMEE DQHLDVHTSQ
410 420 430 440 450
SPTQTEMEEA VEEHVATKTQ LGHVQTELQD AESLQKDFED AKAAAEEAKE
460 470 480 490 500
KEKDLHAISA ELQKEDSDEP TLKRKRTRTL KASQAAKIEP VPSEVKTKVQ
510 520 530 540 550
SGKGALRRIR GKDKEEKVKP PKPKCRCGIS GSSNTLTTCR NSRCPCYKSY
560 570 580 590 600
NSCAGCHCVC CKNPHKEDYV ESDEDDDLED FEMPKDVPEP MTQSEEPVVA
610 620 630 640 650
EPRQEENSMA PPDSSAPISL VPLNNLQQSQ HPLVLVQNEK GEYQGFNIFQ
660 670 680 690 700
GSKPLDPVTV GFTIRVQLQH TDGFGSLPQY AYIMPTIDPP NPPAPSLSPP
710 720 730 740 750
PPPAPDREVI EPPAKKFRTS RTRRGRANFS ALDTVDELVS GGSRSNSAAG
760 770
DRSSATDNAH SLFEEIMSGS DDL
Length:773
Mass (Da):84,856
Last modified:September 27, 2005 - v2
Checksum:i31830142AD64F4D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti280T → N in CAA88358 (PubMed:7796814).Curated1
Sequence conflicti367 – 370Missing (PubMed:7796814).Curated4
Sequence conflicti371 – 373VEE → LKT in AAA75573 (PubMed:7781064).Curated3
Sequence conflicti380P → Q in CAA88358 (PubMed:7796814).Curated1
Sequence conflicti403 – 405TQT → IQA in CAA88358 (PubMed:7796814).Curated3
Sequence conflicti423H → Q in CAA61529 (PubMed:7588059).Curated1
Sequence conflicti429Q → H (PubMed:7796814).Curated1
Sequence conflicti431 – 433AES → EEP (PubMed:7796814).Curated3
Sequence conflicti431 – 433AES → EEP (PubMed:7588059).Curated3
Sequence conflicti439E → V in CAA88358 (PubMed:7796814).Curated1
Sequence conflicti443A → P in CAA88358 (PubMed:7796814).Curated1
Sequence conflicti471T → A in CAA61529 (PubMed:7588059).Curated1
Sequence conflicti591M → L in CAA61529 (PubMed:7588059).Curated1
Sequence conflicti642E → D in CAA88358 (PubMed:7796814).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42553 Genomic DNA. Translation: AAA75573.1.
Z48443 Genomic DNA. Translation: CAA88358.1.
X89241 Genomic DNA. Translation: CAA61529.1.
AE014134 Genomic DNA. Translation: AAF51104.1.
AY128426 mRNA. Translation: AAM75019.1.
PIRiS55554.
RefSeqiNP_523467.1. NM_078743.5.
NP_722907.1. NM_164536.2.
UniGeneiDm.14311.

Genome annotation databases

EnsemblMetazoaiFBtr0077570; FBpp0077259; FBgn0005616.
FBtr0077571; FBpp0077260; FBgn0005616.
GeneIDi33565.
KEGGidme:Dmel_CG3241.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42553 Genomic DNA. Translation: AAA75573.1.
Z48443 Genomic DNA. Translation: CAA88358.1.
X89241 Genomic DNA. Translation: CAA61529.1.
AE014134 Genomic DNA. Translation: AAF51104.1.
AY128426 mRNA. Translation: AAM75019.1.
PIRiS55554.
RefSeqiNP_523467.1. NM_078743.5.
NP_722907.1. NM_164536.2.
UniGeneiDm.14311.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LUANMR-A520-570[»]
4RKGX-ray2.50A/B520-570[»]
4RKHX-ray2.00C/D/E/F520-570[»]
ProteinModelPortaliP50534.
SMRiP50534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59776. 10 interactors.
IntActiP50534. 1 interactor.
STRINGi7227.FBpp0077259.

Proteomic databases

PaxDbiP50534.
PRIDEiP50534.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0077570; FBpp0077259; FBgn0005616.
FBtr0077571; FBpp0077260; FBgn0005616.
GeneIDi33565.
KEGGidme:Dmel_CG3241.

Organism-specific databases

CTDi33565.
FlyBaseiFBgn0005616. msl-2.

Phylogenomic databases

eggNOGiENOG410IGUA. Eukaryota.
ENOG410ZJAZ. LUCA.
GeneTreeiENSGT00390000016814.
InParanoidiP50534.
KOiK13164.
OMAiCEDCSDF.
OrthoDBiEOG091G0IAA.
PhylomeDBiP50534.

Enzyme and pathway databases

ReactomeiR-DME-3214847. HATs acetylate histones.

Miscellaneous databases

GenomeRNAii33565.
PROiP50534.

Gene expression databases

BgeeiFBgn0005616.
ExpressionAtlasiP50534. baseline.
GenevisibleiP50534. DM.

Family and domain databases

CDDicd13122. MSL2_CXC. 1 hit.
InterProiIPR032049. Msl2-CXC.
IPR032043. Msl2_Znf-RING.
IPR033467. Tesmin/TSO1-like_CXC.
IPR001841. Znf_RING.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF16682. MSL2-CXC. 1 hit.
PF16685. zf-RING_10. 1 hit.
[Graphical view]
SMARTiSM01114. CXC. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSL2_DROME
AccessioniPrimary (citable) accession number: P50534
Secondary accession number(s): Q9VQR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 27, 2005
Last modified: November 30, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.