Reviewed, UniProtKB/Swiss-Prot P50529 (STHA_VIBCH)

Names and origin

Protein names

Recommended name:
    Soluble pyridine nucleotide transhydrogenase
      Short name=STH
    EC=1.6.1.1
Alternative name(s):
    NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Synonyms:	udhA
Ordered Locus Names:	VC_0151

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity.
Catalytic activity	NADPH + NAD(+) = NADP(+) + NADH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADP metabolic process Inferred from electronic annotation. Source: HAMAP cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: HAMAP electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Soluble pyridine nucleotide transhydrogenase		PRO_0000068075
Regions
	Nucleotide binding	36 – 45	10	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50529-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 60D05DB3FD595BE1	FASTA	466	50,942
	10 20 30 40 50 60 MGQKNHFDVI VIGSGPGGEG AAMGLTKGGK NVAIIEKESS VGGGCTHWGT IPSKALRHAV 70 80 90 100 110 120 SRIIEFNSNP LFCKNNSSIH ATFSTILSHA KSVIDKQTRL RQGFYDRNQC TLIFGAAHFI 130 140 150 160 170 180 DAHTVAVKKA DGSIDTYSAD KFVIATGSRP YHPKDVDFGH PRIYDSDSIL NLEHDPRHII 190 200 210 220 230 240 IYGAGVIGCE YASIFRGLDV KTDLINTRDR LLSFLDNEVS DALSYHFWNS GVVIRNDETY 250 260 270 280 290 300 DKVEGTSDGV IVHLKSGKKM RADCLLYANG RTGNTDKLNL ESVGLQADSR GQLVVNANYQ 310 320 330 340 350 360 TQVEHIYAVG DVIGYPSLAS AAYDQGRFVA QAIIHGQAAH LLTEDIPTGI YTIPEISSVG 370 380 390 400 410 420 RTEQELTAAK VPYEVGRASF KHLARAQIAG KDIGSLKILF HRETKEILGI HCFGERAAEI 430 440 450 460 IHIGQAIMEQ KGEANTIEYF VNTTFNYPTM AEAFRVAALN GLNRLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.
[2]	"The rpoH gene encoding sigma 32 homolog of Vibrio cholerae." Sahu G.K., Chowdhury R., Das J. Gene 189:203-207(1997) [PubMed: 9168128] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 421-466. Strain: ATCC 25870 / Classical Inaba 569B / Serotype O1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE003852 Genomic DNA. Translation: AAF93328.1. U44432 Genomic DNA. Translation: AAC45342.1.
PIR	E82357.
RefSeq	NP_229809.1.
3D structure databases
HSSP	HSSP built from PDB template 3LAD based on UniProtKB P18925.
ModBase	Search...
Genome annotation databases
GeneID	2614850.
GenomeReviews	Gene locus VC_0151 in contig AE003852_GR.
KEGG	vch:VC0151.
TIGR	VC_0151.
Phylogenomic databases
HOGENOM	P50529.
Family and domain databases
HAMAP	MF_00247. [Tree]
InterPro	IPR000759. Adrndx_reductase. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000815. Hg_reductase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR001327. Pyr_OxRdtase_NAD_bd. IPR003042. Rng_hydrolase. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00419. ADXRDTASE. PR00368. FADPNR. PR00945. HGRDTASE. PR00411. PNDRDTASEI. PR00420. RNGMNOXGNASE.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

STHA_VIBCH

Accession

Primary (citable) accession number: P50529

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 1, 2000
Last modified:	November 25, 2008
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)