SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P50527

- STE20_SCHPO

UniProt

P50527 - STE20_SCHPO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Serine/threonine-protein kinase shk1/pak1
Gene
shk1, orb2, pak1, ste20, SPBC1604.14c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

MAP4K component of the MAPK pathway required for the mating pheromone response. Phosphorylates histone H2B to form H2BS10ph By similarity. Phosphorylates tea1. Required for skb1-dependent mitotic inhibitory function. Regulates microtubule dynamics and cell polarity.7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei415 – 4151ATP By similarity
Active sitei505 – 5051Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi392 – 4009ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein binding Source: PomBase
  3. protein serine/threonine kinase activity Source: PomBase

GO - Biological processi

  1. Cdc42 protein signal transduction Source: PomBase
  2. actin filament organization Source: InterPro
  3. cellular protein localization Source: PomBase
  4. establishment or maintenance of actin cytoskeleton polarity Source: PomBase
  5. establishment or maintenance of bipolar cell polarity regulating cell shape Source: PomBase
  6. positive regulation of establishment of bipolar cell polarity regulating cell shape Source: PomBase
  7. regulation of cytokinesis Source: PomBase
  8. regulation of microtubule cytoskeleton organization Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5615.
ReactomeiREACT_188593. Regulation of actin dynamics for phagocytic cup formation.
REACT_81558. Regulation of activated PAK-2p34 by proteasome mediated degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase shk1/pak1 (EC:2.7.11.1)
Gene namesi
Name:shk1
Synonyms:orb2, pak1, ste20
ORF Names:SPBC1604.14c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC1604.14c.

Subcellular locationi

Cytoplasm. Cytoplasmcytoskeletonspindle
Note: Localizes at cell ends, septum forming regions and at the mitotic spindle.1 Publication

GO - Cellular componenti

  1. Cdc42 GTPase complex Source: PomBase
  2. cell division site Source: PomBase
  3. cell tip Source: PomBase
  4. cytosol Source: PomBase
  5. spindle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 658658Serine/threonine-protein kinase shk1/pak1
PRO_0000086651Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei301 – 3011Phosphoserine1 Publication
Modified residuei303 – 3031Phosphoserine1 Publication

Post-translational modificationi

Autophosphorylated on serine residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP50527.

Interactioni

Subunit structurei

Forms an activated complex with GTP-bound ras-like cdc42. Interacts with skb1 and the SH3 domain of skb5 via its amino-terminal regulatory domain. Skb1, cdc42 and shk1 are able to form a ternary complex in vivo. Interacts with rga8 and may interact with byr2.7 Publications

Protein-protein interaction databases

BioGridi276541. 22 interactions.
DIPiDIP-949N.
IntActiP50527. 1 interaction.
MINTiMINT-111182.
STRINGi4896.SPBC1604.14c-1.

Structurei

3D structure databases

ProteinModelPortaliP50527.
SMRiP50527. Positions 146-189, 365-652.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini147 – 16014CRIB
Add
BLAST
Domaini386 – 637252Protein kinase
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi85 – 884Poly-Ser
Compositional biasi234 – 2374Poly-Ser
Compositional biasi246 – 2494Poly-Ser
Compositional biasi263 – 2675Poly-Ser

Sequence similaritiesi

Contains 1 CRIB domain.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000234202.
KOiK04409.
OMAiVQARGPK.
OrthoDBiEOG708W7W.
PhylomeDBiP50527.

Family and domain databases

Gene3Di3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR011026. WASP_C.
[Graphical view]
PfamiPF00786. PBD. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50527-1 [UniParc]FASTAAdd to Basket

« Hide

MERGTLQPRK KAPNGYGITP IVAHKTGEPV RYEVEDDLRK LKPSRTAPKP    50
PAINTNLAED TFSGFPLSQS RTTVSRVSLG SRQHSSSSIR KLQTNVSDVR 100
SYDERNQKKS AFENFVSSMS SFLTGGGSSP TSSYGSGSAS PRKSTVISSP 150
FDPKHVTHVG FNYDTGEFTG MPTEWQALLK VSGITKSEQV QHPQAVLDAM 200
AFYSQSKKYL EEGAKPPFPR ESTEKPLLSV SALSSSSHLQ PTSATSSSSR 250
LYPSRPAPTP PASSSSSPLL SSQTVKTTTS NASRQPSPLV SSKSTDNIIR 300
SHSPVLLTPQ TLSTSETKHI RPNNSTPYQR RAETSTKPKA VATPQKVEAP 350
SAPRLQKRAP RQQSNDSAVL AKLQSICNPK NPTLLYRNFV KIGQGASGDV 400
YSARQVGTNL SVAIKKMNIN QQPKKEFIVN EILVMKSHHH KNIVNFIDTF 450
FYKSELWMVM EYMRGGSLTE VVTNNTLSEG QIAAICKETL EGLQHLHENG 500
IVHRDIKSDN ILLSLQGDIK LTDFGFCAQI DSNMTKRTTM VGTPYWMAPE 550
VVTRKEYGFK VDVWSLGIMA IEMVEGEPPY LNENPLRALY LIATIGTPKI 600
SRPELLSSVF HDFLSKSLTV NPKQRPSSGE LLRHPFLKQA VPVSSLIPLI 650
KSIHHSGK 658
Length:658
Mass (Da):72,358
Last modified:May 30, 2000 - v2
Checksum:i69D72E5C925021E5
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti492 – 4954GLQH → LYSD in AAC49125. 1 Publication
Sequence conflicti537 – 5371R → P in AAC49125. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U22371 mRNA. Translation: AAC49125.1.
L41552 Genomic DNA. Translation: AAB52609.1.
CU329671 Genomic DNA. Translation: CAA22347.1.
PIRiS60170.
T39500.
RefSeqiNP_596626.1. NM_001022547.2.

Genome annotation databases

EnsemblFungiiSPBC1604.14c.1; SPBC1604.14c.1:pep; SPBC1604.14c.
GeneIDi2539997.
KEGGispo:SPBC1604.14c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U22371 mRNA. Translation: AAC49125.1 .
L41552 Genomic DNA. Translation: AAB52609.1 .
CU329671 Genomic DNA. Translation: CAA22347.1 .
PIRi S60170.
T39500.
RefSeqi NP_596626.1. NM_001022547.2.

3D structure databases

ProteinModelPortali P50527.
SMRi P50527. Positions 146-189, 365-652.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 276541. 22 interactions.
DIPi DIP-949N.
IntActi P50527. 1 interaction.
MINTi MINT-111182.
STRINGi 4896.SPBC1604.14c-1.

Proteomic databases

MaxQBi P50527.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC1604.14c.1 ; SPBC1604.14c.1:pep ; SPBC1604.14c .
GeneIDi 2539997.
KEGGi spo:SPBC1604.14c.

Organism-specific databases

PomBasei SPBC1604.14c.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000234202.
KOi K04409.
OMAi VQARGPK.
OrthoDBi EOG708W7W.
PhylomeDBi P50527.

Enzyme and pathway databases

BRENDAi 2.7.11.1. 5615.
Reactomei REACT_188593. Regulation of actin dynamics for phagocytic cup formation.
REACT_81558. Regulation of activated PAK-2p34 by proteasome mediated degradation.

Miscellaneous databases

NextBioi 20801140.

Family and domain databases

Gene3Di 3.90.810.10. 1 hit.
InterProi IPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR011026. WASP_C.
[Graphical view ]
Pfami PF00786. PBD. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00285. PBD. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF47912. SSF47912. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS50108. CRIB. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Fission yeast pak1+ encodes a protein kinase that interacts with Cdc42p and is involved in the control of cell polarity and mating."
    Ottilie S., Miller P.J., Johnson D.I., Creasy C.L., Sells M.A., Bagrodia S., Forsburg S.L., Chernoff J.
    EMBO J. 14:5908-5919(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CDC42, FUNCTION.
  2. Marcus S.
    Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "Shk1, a homolog of the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe."
    Marcus S., Polverino A., Chang E., Robbins D., Cobb M.H., Wigler M.
    Proc. Natl. Acad. Sci. U.S.A. 92:6180-6184(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 119-658, INTERACTION WITH CDC42, FUNCTION.
  5. "The highly conserved skb1 gene encodes a protein that interacts with Shk1, a fission yeast Ste20/PAK homolog."
    Gilbreth M., Yang P., Wang D., Frost J., Polverino A., Cobb M.H., Marcus S.
    Proc. Natl. Acad. Sci. U.S.A. 93:13802-13807(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SKB1.
  6. "Negative regulation of mitosis in fission yeast by the shk1 interacting protein skb1 and its human homolog, Skb1Hs."
    Gilbreth M., Yang P., Bartholomeusz G., Pimental R.A., Kansra S., Gadiraju R., Marcus S.
    Proc. Natl. Acad. Sci. U.S.A. 95:14781-14786(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC42.
  7. "Mis-specification of cortical identity in a fission yeast PAK mutant."
    Sawin K.E., Hajibagheri M.A.N., Nurse P.
    Curr. Biol. 9:1335-1338(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Direct activation of the fission yeast PAK Shk1 by the novel SH3 domain protein, Skb5."
    Yang P., Pimental R.A., Lai H., Marcus S.
    J. Biol. Chem. 274:36052-36057(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKB5.
  9. "Direct binding and In vivo regulation of the fission yeast p21-activated kinase shk1 by the SH3 domain protein scd2."
    Chang E., Bartholomeusz G., Pimental R.A., Chen J., Lai H., Wang L.L., Yang P., Marcus S.
    Mol. Cell. Biol. 19:8066-8074(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SCD2, AUTOPHOSPHORYLATION.
  10. "The p21-activated kinase, Shk1, is required for proper regulation of microtubule dynamics in the fission yeast, Schizosaccharomyces pombe."
    Qyang Y., Yang P., Du H., Lai H., Kim H., Marcus S.
    Mol. Microbiol. 44:325-334(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  11. "The kelch repeat protein, Tea1, is a potential substrate target of the p21-activated kinase, Shk1, in the fission yeast, Schizosaccharomyces pombe."
    Kim H., Yang P., Catanuto P., Verde F., Lai H., Du H., Chang F., Marcus S.
    J. Biol. Chem. 278:30074-30082(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PHOSPHORYLATION OF TEA1.
  12. "The novel Rho GTPase-activating protein family protein, Rga8, provides a potential link between Cdc42/p21-activated kinase and Rho signaling pathways in the fission yeast, Schizosaccharomyces pombe."
    Yang P., Qyang Y., Bartholomeusz G., Zhou X., Marcus S.
    J. Biol. Chem. 278:48821-48830(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RGA8.
  13. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-303, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSTE20_SCHPO
AccessioniPrimary (citable) accession number: P50527
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: September 3, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi