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Protein

Serine/threonine-protein kinase shk1/pak1

Gene

shk1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

MAP4K component of the MAPK pathway required for the mating pheromone response. Phosphorylates histone H2B to form H2BS10ph (By similarity). Phosphorylates tea1. Required for skb1-dependent mitotic inhibitory function. Regulates microtubule dynamics and cell polarity.By similarity7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei415ATPPROSITE-ProRule annotation1
Active sitei505Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi392 – 400ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: PomBase
  • protein serine/threonine kinase activity Source: PomBase
  • Rac GTPase binding Source: GO_Central

GO - Biological processi

  • actin cytoskeleton organization Source: GO_Central
  • actin filament organization Source: InterPro
  • activation of protein kinase activity Source: GO_Central
  • Cdc42 protein signal transduction Source: PomBase
  • negative regulation of septation initiation signaling Source: PomBase
  • positive regulation of establishment of bipolar cell polarity regulating cell shape Source: PomBase
  • positive regulation of establishment or maintenance of bipolar cell polarity regulating cell shape Source: PomBase
  • regulation of apoptotic process Source: GO_Central
  • regulation of MAPK cascade Source: GO_Central
  • regulation of microtubule cytoskeleton organization Source: PomBase
  • signal transduction by protein phosphorylation Source: GO_Central
  • stress-activated protein kinase signaling cascade Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5613.
ReactomeiR-SPO-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-SPO-389359. CD28 dependent Vav1 pathway.
R-SPO-5627123. RHO GTPases activate PAKs.
R-SPO-5687128. MAPK6/MAPK4 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase shk1/pak1 (EC:2.7.11.1)
Gene namesi
Name:shk1
Synonyms:orb2, pak1, ste20
ORF Names:SPBC1604.14c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC1604.14c.
PomBaseiSPBC1604.14c. shk1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000866511 – 658Serine/threonine-protein kinase shk1/pak1Add BLAST658

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei301Phosphoserine1 Publication1
Modified residuei303Phosphoserine1 Publication1

Post-translational modificationi

Autophosphorylated on serine residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP50527.
PRIDEiP50527.

PTM databases

iPTMnetiP50527.

Interactioni

Subunit structurei

Forms an activated complex with GTP-bound ras-like cdc42. Interacts with skb1 and the SH3 domain of skb5 via its amino-terminal regulatory domain. Skb1, cdc42 and shk1 are able to form a ternary complex in vivo. Interacts with rga8 and may interact with byr2.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
scd2P409963EBI-767318,EBI-767302

GO - Molecular functioni

Protein-protein interaction databases

BioGridi276541. 21 interactors.
DIPiDIP-949N.
IntActiP50527. 3 interactors.
MINTiMINT-111182.
STRINGi4896.SPBC1604.14c.1.

Structurei

3D structure databases

ProteinModelPortaliP50527.
SMRiP50527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini147 – 160CRIBPROSITE-ProRule annotationAdd BLAST14
Domaini386 – 637Protein kinasePROSITE-ProRule annotationAdd BLAST252

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi85 – 88Poly-Ser4
Compositional biasi234 – 237Poly-Ser4
Compositional biasi246 – 249Poly-Ser4
Compositional biasi263 – 267Poly-Ser5

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000234202.
InParanoidiP50527.
KOiK04409.
OMAiAGTLNHG.
OrthoDBiEOG092C2E30.
PhylomeDBiP50527.

Family and domain databases

CDDicd01093. CRIB_PAK_like. 1 hit.
Gene3Di3.90.810.10. 1 hit.
InterProiView protein in InterPro
IPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR033923. PAK_BD.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR011026. WASP_C.
PfamiView protein in Pfam
PF00786. PBD. 1 hit.
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00285. PBD. 1 hit.
SM00220. S_TKc. 1 hit.
SUPFAMiSSF47912. SSF47912. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50108. CRIB. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

P50527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERGTLQPRK KAPNGYGITP IVAHKTGEPV RYEVEDDLRK LKPSRTAPKP
60 70 80 90 100
PAINTNLAED TFSGFPLSQS RTTVSRVSLG SRQHSSSSIR KLQTNVSDVR
110 120 130 140 150
SYDERNQKKS AFENFVSSMS SFLTGGGSSP TSSYGSGSAS PRKSTVISSP
160 170 180 190 200
FDPKHVTHVG FNYDTGEFTG MPTEWQALLK VSGITKSEQV QHPQAVLDAM
210 220 230 240 250
AFYSQSKKYL EEGAKPPFPR ESTEKPLLSV SALSSSSHLQ PTSATSSSSR
260 270 280 290 300
LYPSRPAPTP PASSSSSPLL SSQTVKTTTS NASRQPSPLV SSKSTDNIIR
310 320 330 340 350
SHSPVLLTPQ TLSTSETKHI RPNNSTPYQR RAETSTKPKA VATPQKVEAP
360 370 380 390 400
SAPRLQKRAP RQQSNDSAVL AKLQSICNPK NPTLLYRNFV KIGQGASGDV
410 420 430 440 450
YSARQVGTNL SVAIKKMNIN QQPKKEFIVN EILVMKSHHH KNIVNFIDTF
460 470 480 490 500
FYKSELWMVM EYMRGGSLTE VVTNNTLSEG QIAAICKETL EGLQHLHENG
510 520 530 540 550
IVHRDIKSDN ILLSLQGDIK LTDFGFCAQI DSNMTKRTTM VGTPYWMAPE
560 570 580 590 600
VVTRKEYGFK VDVWSLGIMA IEMVEGEPPY LNENPLRALY LIATIGTPKI
610 620 630 640 650
SRPELLSSVF HDFLSKSLTV NPKQRPSSGE LLRHPFLKQA VPVSSLIPLI

KSIHHSGK
Length:658
Mass (Da):72,358
Last modified:May 30, 2000 - v2
Checksum:i69D72E5C925021E5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti492 – 495GLQH → LYSD in AAC49125 (PubMed:8846783).Curated4
Sequence conflicti537R → P in AAC49125 (PubMed:8846783).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22371 mRNA. Translation: AAC49125.1.
L41552 Genomic DNA. Translation: AAB52609.1.
CU329671 Genomic DNA. Translation: CAA22347.1.
PIRiS60170.
T39500.
RefSeqiNP_596626.1. NM_001022547.2.

Genome annotation databases

EnsemblFungiiSPBC1604.14c.1; SPBC1604.14c.1:pep; SPBC1604.14c.
GeneIDi2539997.
KEGGispo:SPBC1604.14c.

Similar proteinsi

Entry informationi

Entry nameiSTE20_SCHPO
AccessioniPrimary (citable) accession number: P50527
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: September 27, 2017
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families