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P50527

- STE20_SCHPO

UniProt

P50527 - STE20_SCHPO

Protein

Serine/threonine-protein kinase shk1/pak1

Gene

shk1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    MAP4K component of the MAPK pathway required for the mating pheromone response. Phosphorylates histone H2B to form H2BS10ph By similarity. Phosphorylates tea1. Required for skb1-dependent mitotic inhibitory function. Regulates microtubule dynamics and cell polarity.By similarity7 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei415 – 4151ATPPROSITE-ProRule annotation
    Active sitei505 – 5051Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi392 – 4009ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: PomBase
    3. protein serine/threonine kinase activity Source: PomBase

    GO - Biological processi

    1. actin filament organization Source: InterPro
    2. Cdc42 protein signal transduction Source: PomBase
    3. cellular protein localization Source: PomBase
    4. establishment or maintenance of actin cytoskeleton polarity Source: PomBase
    5. establishment or maintenance of bipolar cell polarity regulating cell shape Source: PomBase
    6. positive regulation of establishment of bipolar cell polarity regulating cell shape Source: PomBase
    7. regulation of microtubule cytoskeleton organization Source: PomBase
    8. regulation of mitotic cytokinesis Source: PomBase

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.1. 5615.
    ReactomeiREACT_188593. Regulation of actin dynamics for phagocytic cup formation.
    REACT_81558. Regulation of activated PAK-2p34 by proteasome mediated degradation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase shk1/pak1 (EC:2.7.11.1)
    Gene namesi
    Name:shk1
    Synonyms:orb2, pak1, ste20
    ORF Names:SPBC1604.14c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC1604.14c.

    Subcellular locationi

    Cytoplasm 1 Publication. Cytoplasmcytoskeletonspindle 1 Publication
    Note: Localizes at cell ends, septum forming regions and at the mitotic spindle.

    GO - Cellular componenti

    1. Cdc42 GTPase complex Source: PomBase
    2. cell division site Source: PomBase
    3. cell tip Source: PomBase
    4. cytosol Source: PomBase
    5. spindle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 658658Serine/threonine-protein kinase shk1/pak1PRO_0000086651Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei301 – 3011Phosphoserine1 Publication
    Modified residuei303 – 3031Phosphoserine1 Publication

    Post-translational modificationi

    Autophosphorylated on serine residues.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP50527.

    Interactioni

    Subunit structurei

    Forms an activated complex with GTP-bound ras-like cdc42. Interacts with skb1 and the SH3 domain of skb5 via its amino-terminal regulatory domain. Skb1, cdc42 and shk1 are able to form a ternary complex in vivo. Interacts with rga8 and may interact with byr2.7 Publications

    Protein-protein interaction databases

    BioGridi276541. 22 interactions.
    DIPiDIP-949N.
    IntActiP50527. 1 interaction.
    MINTiMINT-111182.
    STRINGi4896.SPBC1604.14c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP50527.
    SMRiP50527. Positions 146-189, 365-652.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini147 – 16014CRIBPROSITE-ProRule annotationAdd
    BLAST
    Domaini386 – 637252Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi85 – 884Poly-Ser
    Compositional biasi234 – 2374Poly-Ser
    Compositional biasi246 – 2494Poly-Ser
    Compositional biasi263 – 2675Poly-Ser

    Sequence similaritiesi

    Contains 1 CRIB domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000234202.
    KOiK04409.
    OMAiVQARGPK.
    OrthoDBiEOG708W7W.
    PhylomeDBiP50527.

    Family and domain databases

    Gene3Di3.90.810.10. 1 hit.
    InterProiIPR000095. CRIB_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR011026. WASP_C.
    [Graphical view]
    PfamiPF00786. PBD. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00285. PBD. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF47912. SSF47912. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS50108. CRIB. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50527-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERGTLQPRK KAPNGYGITP IVAHKTGEPV RYEVEDDLRK LKPSRTAPKP    50
    PAINTNLAED TFSGFPLSQS RTTVSRVSLG SRQHSSSSIR KLQTNVSDVR 100
    SYDERNQKKS AFENFVSSMS SFLTGGGSSP TSSYGSGSAS PRKSTVISSP 150
    FDPKHVTHVG FNYDTGEFTG MPTEWQALLK VSGITKSEQV QHPQAVLDAM 200
    AFYSQSKKYL EEGAKPPFPR ESTEKPLLSV SALSSSSHLQ PTSATSSSSR 250
    LYPSRPAPTP PASSSSSPLL SSQTVKTTTS NASRQPSPLV SSKSTDNIIR 300
    SHSPVLLTPQ TLSTSETKHI RPNNSTPYQR RAETSTKPKA VATPQKVEAP 350
    SAPRLQKRAP RQQSNDSAVL AKLQSICNPK NPTLLYRNFV KIGQGASGDV 400
    YSARQVGTNL SVAIKKMNIN QQPKKEFIVN EILVMKSHHH KNIVNFIDTF 450
    FYKSELWMVM EYMRGGSLTE VVTNNTLSEG QIAAICKETL EGLQHLHENG 500
    IVHRDIKSDN ILLSLQGDIK LTDFGFCAQI DSNMTKRTTM VGTPYWMAPE 550
    VVTRKEYGFK VDVWSLGIMA IEMVEGEPPY LNENPLRALY LIATIGTPKI 600
    SRPELLSSVF HDFLSKSLTV NPKQRPSSGE LLRHPFLKQA VPVSSLIPLI 650
    KSIHHSGK 658
    Length:658
    Mass (Da):72,358
    Last modified:May 30, 2000 - v2
    Checksum:i69D72E5C925021E5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti492 – 4954GLQH → LYSD in AAC49125. (PubMed:8846783)Curated
    Sequence conflicti537 – 5371R → P in AAC49125. (PubMed:8846783)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22371 mRNA. Translation: AAC49125.1.
    L41552 Genomic DNA. Translation: AAB52609.1.
    CU329671 Genomic DNA. Translation: CAA22347.1.
    PIRiS60170.
    T39500.
    RefSeqiNP_596626.1. NM_001022547.2.

    Genome annotation databases

    EnsemblFungiiSPBC1604.14c.1; SPBC1604.14c.1:pep; SPBC1604.14c.
    GeneIDi2539997.
    KEGGispo:SPBC1604.14c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22371 mRNA. Translation: AAC49125.1 .
    L41552 Genomic DNA. Translation: AAB52609.1 .
    CU329671 Genomic DNA. Translation: CAA22347.1 .
    PIRi S60170.
    T39500.
    RefSeqi NP_596626.1. NM_001022547.2.

    3D structure databases

    ProteinModelPortali P50527.
    SMRi P50527. Positions 146-189, 365-652.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276541. 22 interactions.
    DIPi DIP-949N.
    IntActi P50527. 1 interaction.
    MINTi MINT-111182.
    STRINGi 4896.SPBC1604.14c-1.

    Proteomic databases

    MaxQBi P50527.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC1604.14c.1 ; SPBC1604.14c.1:pep ; SPBC1604.14c .
    GeneIDi 2539997.
    KEGGi spo:SPBC1604.14c.

    Organism-specific databases

    PomBasei SPBC1604.14c.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000234202.
    KOi K04409.
    OMAi VQARGPK.
    OrthoDBi EOG708W7W.
    PhylomeDBi P50527.

    Enzyme and pathway databases

    BRENDAi 2.7.11.1. 5615.
    Reactomei REACT_188593. Regulation of actin dynamics for phagocytic cup formation.
    REACT_81558. Regulation of activated PAK-2p34 by proteasome mediated degradation.

    Miscellaneous databases

    NextBioi 20801140.

    Family and domain databases

    Gene3Di 3.90.810.10. 1 hit.
    InterProi IPR000095. CRIB_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR011026. WASP_C.
    [Graphical view ]
    Pfami PF00786. PBD. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00285. PBD. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47912. SSF47912. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS50108. CRIB. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Fission yeast pak1+ encodes a protein kinase that interacts with Cdc42p and is involved in the control of cell polarity and mating."
      Ottilie S., Miller P.J., Johnson D.I., Creasy C.L., Sells M.A., Bagrodia S., Forsburg S.L., Chernoff J.
      EMBO J. 14:5908-5919(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CDC42, FUNCTION.
    2. Marcus S.
      Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    4. "Shk1, a homolog of the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe."
      Marcus S., Polverino A., Chang E., Robbins D., Cobb M.H., Wigler M.
      Proc. Natl. Acad. Sci. U.S.A. 92:6180-6184(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 119-658, INTERACTION WITH CDC42, FUNCTION.
    5. "The highly conserved skb1 gene encodes a protein that interacts with Shk1, a fission yeast Ste20/PAK homolog."
      Gilbreth M., Yang P., Wang D., Frost J., Polverino A., Cobb M.H., Marcus S.
      Proc. Natl. Acad. Sci. U.S.A. 93:13802-13807(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SKB1.
    6. "Negative regulation of mitosis in fission yeast by the shk1 interacting protein skb1 and its human homolog, Skb1Hs."
      Gilbreth M., Yang P., Bartholomeusz G., Pimental R.A., Kansra S., Gadiraju R., Marcus S.
      Proc. Natl. Acad. Sci. U.S.A. 95:14781-14786(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC42.
    7. "Mis-specification of cortical identity in a fission yeast PAK mutant."
      Sawin K.E., Hajibagheri M.A.N., Nurse P.
      Curr. Biol. 9:1335-1338(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Direct activation of the fission yeast PAK Shk1 by the novel SH3 domain protein, Skb5."
      Yang P., Pimental R.A., Lai H., Marcus S.
      J. Biol. Chem. 274:36052-36057(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SKB5.
    9. "Direct binding and In vivo regulation of the fission yeast p21-activated kinase shk1 by the SH3 domain protein scd2."
      Chang E., Bartholomeusz G., Pimental R.A., Chen J., Lai H., Wang L.L., Yang P., Marcus S.
      Mol. Cell. Biol. 19:8066-8074(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SCD2, AUTOPHOSPHORYLATION.
    10. "The p21-activated kinase, Shk1, is required for proper regulation of microtubule dynamics in the fission yeast, Schizosaccharomyces pombe."
      Qyang Y., Yang P., Du H., Lai H., Kim H., Marcus S.
      Mol. Microbiol. 44:325-334(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    11. "The kelch repeat protein, Tea1, is a potential substrate target of the p21-activated kinase, Shk1, in the fission yeast, Schizosaccharomyces pombe."
      Kim H., Yang P., Catanuto P., Verde F., Lai H., Du H., Chang F., Marcus S.
      J. Biol. Chem. 278:30074-30082(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHORYLATION OF TEA1.
    12. "The novel Rho GTPase-activating protein family protein, Rga8, provides a potential link between Cdc42/p21-activated kinase and Rho signaling pathways in the fission yeast, Schizosaccharomyces pombe."
      Yang P., Qyang Y., Bartholomeusz G., Zhou X., Marcus S.
      J. Biol. Chem. 278:48821-48830(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RGA8.
    13. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301 AND SER-303, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiSTE20_SCHPO
    AccessioniPrimary (citable) accession number: P50527
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 137 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3