SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P50525

- APN1_SCHPO

UniProt

P50525 - APN1_SCHPO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
DNA-(apurinic or apyrimidinic site) lyase 1
Gene
apn1, SPCC622.17
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Protein uncertaini

Functioni

DNA repair enzyme that cleaves apurinic/apyrimidinic (AP) sites and removes 3'-blocking groups present at single strand breaks of damaged DNA. Provides back-up AP endonuclease (APE) activity to apn2 together with uve1.2 Publications

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

Binds 3 zinc ions By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Zinc 1 By similarity
Metal bindingi136 – 1361Zinc 1 By similarity
Metal bindingi136 – 1361Zinc 2 By similarity
Metal bindingi170 – 1701Zinc 2 By similarity
Metal bindingi173 – 1731Zinc 3 By similarity
Metal bindingi207 – 2071Zinc 2 By similarity
Metal bindingi220 – 2201Zinc 3 By similarity
Metal bindingi222 – 2221Zinc 3 By similarity
Metal bindingi252 – 2521Zinc 2 By similarity

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-(apurinic or apyrimidinic site) lyase 1 (EC:4.2.99.18)
Alternative name(s):
Apurinic-apyrimidinic endonuclease 1
Short name:
AP endonuclease 1
Gene namesi
Name:apn1
ORF Names:SPCC622.17
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome III

Subcellular locationi

Nucleus By similarity UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342DNA-(apurinic or apyrimidinic site) lyase 1UniRule annotation
PRO_0000190897Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi275375. 9 interactions.
MINTiMINT-4690690.
STRINGi4896.SPCC622.17-1.

Structurei

3D structure databases

ProteinModelPortaliP50525.

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.

Phylogenomic databases

eggNOGiCOG0648.
HOGENOMiHOG000224893.
OMAiDEINWIN.
OrthoDBiEOG71VT48.
PhylomeDBiP50525.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50525-1 [UniParc]FASTAAdd to Basket

« Hide

MCAINKAYLL TKFYISANSC AFFVKSQRKW TSPDLSEDVA QKFLETASEM    50
KFDASKQVLV HGSYLINMAN ADEQKREQAF NCFVDDLKRC ERLGVGLYNF 100
HPGSTASCTK EEGINNLAEC INRAHEETKS VIIVTENMAG QGNCLGGTFD 150
DFAALKSKIK NLDRWRVCLD TCHTFAAGYD IRTEESYKKV IDEFDEKVGA 200
KYVSGWHLND SKAPLGSNRD LHENIGLGFL GLEPFRLIMN DSRWDGIPLV 250
LETPAKSPEQ WKKEVELLRF MVGKSSDDVE LMKESARLSN LGAASRKEHL 300
NKFEKKEAKK DRKKKSKDGD QTTLLLRKKQ KLGNAEVKSL DE 342
Length:342
Mass (Da):38,657
Last modified:June 7, 2004 - v3
Checksum:iC6711EC23691C51D
GO

Sequence cautioni

The sequence AAC28163.1 differs from that shown. Reason: Intron retention.
The sequence AAC28163.1 differs from that shown. Reason: Frameshift at position 298.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY483157 mRNA. Translation: AAR83751.1.
CU329672 Genomic DNA. No translation available.
U33625 mRNA. Translation: AAC28163.1. Sequence problems.
PIRiT52563.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY483157 mRNA. Translation: AAR83751.1 .
CU329672 Genomic DNA. No translation available.
U33625 mRNA. Translation: AAC28163.1 . Sequence problems.
PIRi T52563.

3D structure databases

ProteinModelPortali P50525.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 275375. 9 interactions.
MINTi MINT-4690690.
STRINGi 4896.SPCC622.17-1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0648.
HOGENOMi HOG000224893.
OMAi DEINWIN.
OrthoDBi EOG71VT48.
PhylomeDBi P50525.

Miscellaneous databases

NextBioi 20799976.
PROi P50525.

Family and domain databases

Gene3Di 3.20.20.150. 1 hit.
HAMAPi MF_00152. Nfo.
InterProi IPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view ]
PANTHERi PTHR21445. PTHR21445. 1 hit.
Pfami PF01261. AP_endonuc_2. 1 hit.
[Graphical view ]
SMARTi SM00518. AP2Ec. 1 hit.
[Graphical view ]
SUPFAMi SSF51658. SSF51658. 1 hit.
TIGRFAMsi TIGR00587. nfo. 1 hit.
PROSITEi PS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The major role of human AP-endonuclease homolog Apn2 in repair of abasic sites in Schizosaccharomyces pombe."
    Ribar B., Izumi T., Mitra S.
    Nucleic Acids Res. 32:115-126(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. Seeger K., Harris D., Lyne M., Rajandream M.A., Barrell B.G.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. "Schizosaccharomyces pombe apn1 encodes a homologue of the Escherichia coli endonuclease IV family of DNA repair proteins."
    Ramotar D., Vadnais J., Masson J.Y., Tremblay S.
    Biochim. Biophys. Acta 1396:15-20(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-319.
  5. "Schizosaccharomyces pombe encodes a mutated AP endonuclease 1."
    Laerdahl J.K., Korvald H., Nilsen L., Dahl-Michelsen K., Rognes T., Bjoras M., Alseth I.
    DNA Repair 10:296-305(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF FUNCTION.

Entry informationi

Entry nameiAPN1_SCHPO
AccessioniPrimary (citable) accession number: P50525
Secondary accession number(s): Q9USI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 7, 2004
Last modified: May 14, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Could be the product of a pseudogene. This is a truncated version of AP endonuclease 1. In strain 972 and its derivative FY527, this gene has a stop codon in position 5, which disrupts the gene coding for this protein. 1 Publication shows that the truncated protein is not functional. A full sequence for apn1 can be found in strains SPK19802 (S.pombe var. kambucha), NCYC 132, NCYC 683, NCYC 936 and NCYC 2722 (1 Publication).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi