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Protein

26S proteasome regulatory subunit rpn12

Gene

rpn12

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-SPO-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SPO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SPO-68949. Orc1 removal from chromatin.
R-SPO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SPO-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SPO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SPO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit rpn12
Gene namesi
Name:rpn12
Synonyms:mts3
ORF Names:SPBC16G5.01, SPBC342.07
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC16G5.01.
PomBaseiSPBC16G5.01. rpn12.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nuclear chromatin Source: PomBase
  • nuclear periphery Source: PomBase
  • nucleus Source: PomBase
  • proteasome regulatory particle, lid subcomplex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 27027026S proteasome regulatory subunit rpn12PRO_0000173850Add
BLAST

Proteomic databases

MaxQBiP50524.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
cut8P389372EBI-1152607,EBI-1152591

Protein-protein interaction databases

BioGridi276569. 19 interactions.
IntActiP50524. 2 interactions.
MINTiMINT-4690673.

Structurei

Secondary structure

1
270
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 148Combined sources
Helixi18 – 3417Combined sources
Helixi44 – 6320Combined sources
Helixi67 – 8115Combined sources
Helixi91 – 10414Combined sources
Helixi108 – 11710Combined sources
Helixi123 – 1264Combined sources
Helixi128 – 14114Combined sources
Helixi145 – 1539Combined sources
Helixi158 – 1603Combined sources
Helixi161 – 18222Combined sources
Beta strandi184 – 1874Combined sources
Helixi188 – 1947Combined sources
Helixi200 – 21011Combined sources
Beta strandi213 – 2153Combined sources
Beta strandi218 – 2203Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4B0ZX-ray1.58A/B1-224[»]
ProteinModelPortaliP50524.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the proteasome subunit S14 family.Curated

Phylogenomic databases

HOGENOMiHOG000196008.
InParanoidiP50524.
KOiK03031.
OMAiEVPCEEY.
OrthoDBiEOG793BK3.
PhylomeDBiP50524.

Family and domain databases

InterProiIPR006746. 26S_Psome_Rpn12.
IPR033464. CSN8_PSD8_EIF3K.
[Graphical view]
PANTHERiPTHR12387. PTHR12387. 2 hits.
PfamiPF10075. CSN8_PSD8_EIF3K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50524-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTLDLNHLA DLYDRKDWNA CKKELLKLKV ELAKQNLFVP TSDKEKASFA
60 70 80 90 100
RNVFEYGVLV SIQTCDIESF ARYASQVIPF YHDSLVPSSR MGLVTGLNLL
110 120 130 140 150
YLLSENRIAE FHTALESVPD KSLFERDPYV EWVISLEQNV MEGAFDKVAS
160 170 180 190 200
MIRSCNFPEF SYFMKIVMSM VRNEIATCAE KVYSEIPLSN ATSLLYLENT
210 220 230 240 250
KETEKLAEER GWDIRDGVIY FPKEANALET EDGMLIDEED ELELPPTASK
260 270
HTISSIRQLL SYTSELEQIV
Length:270
Mass (Da):30,899
Last modified:October 1, 1996 - v1
Checksum:iC1BC6571722A034D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92682 mRNA. Translation: CAA63366.1.
CU329671 Genomic DNA. Translation: CAB46777.1.
PIRiT40280.
RefSeqiNP_596750.2. NM_001023770.2.

Genome annotation databases

EnsemblFungiiSPBC16G5.01.1; SPBC16G5.01.1:pep; SPBC16G5.01.
GeneIDi2540025.
KEGGispo:SPBC16G5.01.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92682 mRNA. Translation: CAA63366.1.
CU329671 Genomic DNA. Translation: CAB46777.1.
PIRiT40280.
RefSeqiNP_596750.2. NM_001023770.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4B0ZX-ray1.58A/B1-224[»]
ProteinModelPortaliP50524.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276569. 19 interactions.
IntActiP50524. 2 interactions.
MINTiMINT-4690673.

Proteomic databases

MaxQBiP50524.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC16G5.01.1; SPBC16G5.01.1:pep; SPBC16G5.01.
GeneIDi2540025.
KEGGispo:SPBC16G5.01.

Organism-specific databases

EuPathDBiFungiDB:SPBC16G5.01.
PomBaseiSPBC16G5.01. rpn12.

Phylogenomic databases

HOGENOMiHOG000196008.
InParanoidiP50524.
KOiK03031.
OMAiEVPCEEY.
OrthoDBiEOG793BK3.
PhylomeDBiP50524.

Enzyme and pathway databases

ReactomeiR-SPO-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SPO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SPO-68949. Orc1 removal from chromatin.
R-SPO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SPO-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SPO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SPO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiP50524.

Family and domain databases

InterProiIPR006746. 26S_Psome_Rpn12.
IPR033464. CSN8_PSD8_EIF3K.
[Graphical view]
PANTHERiPTHR12387. PTHR12387. 2 hits.
PfamiPF10075. CSN8_PSD8_EIF3K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A conditional lethal mutant in the fission yeast 26 S protease subunit mts3+ is defective in metaphase to anaphase transition."
    Gordon C.B., McGurk G., Wallace M., Hastie N.D.
    J. Biol. Chem. 271:5704-5711(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiRPN12_SCHPO
AccessioniPrimary (citable) accession number: P50524
Secondary accession number(s): Q9UUC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.