Reviewed, UniProtKB/Swiss-Prot P50521 (HXK2_SCHPO)

Names and origin

Protein names

Recommended name:
    Hexokinase-2
    EC=2.7.1.1

Gene names

Name:	hxk2
ORF Names:	SPAC4F8.07c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Pathway	Carbohydrate metabolism; hexose metabolism.
Subunit structure	Monomer.
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Ref.1 Inferred from direct assay. Source: GeneDB_SPombe glucose 6-phosphate metabolic process Ref.1 Inferred from direct assay. Source: GeneDB_SPombe glycolysis Inferred from electronic annotation. Source: UniProtKB-KW mannose metabolic process Ref.1 Inferred from direct assay. Source: GeneDB_SPombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructokinase activity Ref.1 Inferred from direct assay. Source: GeneDB_SPombe glucokinase activity Ref.1 Inferred from direct assay. Source: GeneDB_SPombe mannokinase activity Ref.1 Inferred from direct assay. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	Hexokinase-2		PRO_0000197610
Regions
	Region	138 – 164	27	Glucose-binding Potential
Sites
	Binding site	93	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50521-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 9751F9969F63AC96	FASTA	455	50,920
	10 20 30 40 50 60 MEANFQQAVK KLVNDFEYPT ESLREAVKEF DELRQKGLQK NGEVLAMAPA FISTLPTGAE 70 80 90 100 110 120 TGDFLALDFG GTNLRVCWIQ LLGDGKYEMK HSKSVLPREC VRNESVKPII DFMSDHVELF 130 140 150 160 170 180 IKEHFPSKFG CPEEEYLPMG FTFSYPANQV SITESYLLRW TKGLNIPEAI NKDFAQFLTE 190 200 210 220 230 240 GFKARNLPIR IEAVINDTVG TLVTRAYTSK ESDTFMGIIF GTGTNGAYVE QMNQIPKLAG 250 260 270 280 290 300 KCTGDHMLIN MEWGATDFSC LHSTRYDLLL DHDTPNAGRQ IFEKRVGGMY LGELFRRALF 310 320 330 340 350 360 HLIKVYNFNE GIFPPSITDA WSLETSVLSR MMVERSAENV RNVLSTFKFR FRSDEEALYL 370 380 390 400 410 420 WDAAHAIGRR AARMSAVPIA SLYLSTGRAG KKSDVGVDGS LVEHYPHFVD MLREALRELI 430 440 450 GDNEKLISIG IAKDGSGIGA ALCALQAVKE KKGLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Schizosaccharomyces pombe possesses an unusual and a conventional hexokinase: biochemical and molecular characterization of both hexokinases." Petit T., Blazquez M.A., Gancedo C. FEBS Lett. 378:185-189(1996) [PubMed: 8549830] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X92895 mRNA. Translation: CAA63488.1. CU329670 Genomic DNA. Translation: CAB11054.1.
PIR	S68693.
RefSeq	NP_593865.1.
3D structure databases
HSSP	HSSP built from PDB template 1IG8 based on UniProtKB P04807.
ModBase	Search...
Protein-protein interaction databases
STRING	P50521.
Genome annotation databases
GeneID	2543607.
GenomeReviews	Gene locus hxk2 in contig CU329670_GR.
KEGG	spo:SPAC4F8.07c.
NMPDR	fig|4896.1.peg.3835.
Organism-specific databases
GeneDB_Spombe	SPAC4F8.07c.
Phylogenomic databases
OMA	HIEIDDS.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001515-MON.
BRENDA	2.7.1.1. 653.
Gene expression databases
ArrayExpress	P50521.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR019807. Hexokinase_CS. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
ProDom	PD001109. Hexokinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00378. HEXOKINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HXK2_SCHPO

Accession

Primary (citable) accession number: P50521

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)