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P50520

- VPS34_SCHPO

UniProt

P50520 - VPS34_SCHPO

Protein

Phosphatidylinositol 3-kinase vps34

Gene

vps34

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (14 Aug 2001)
      Previous versions | rss
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    Functioni

    Phosphatidylinositol 3-kinase homolog required for vacuolar sorting and segregation.

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

    GO - Molecular functioni

    1. 1-phosphatidylinositol-3-kinase activity Source: PomBase
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular protein localization Source: PomBase
    2. phosphatidylinositol-3-phosphate biosynthetic process Source: PomBase
    3. phosphatidylinositol-mediated signaling Source: InterPro
    4. phosphatidylinositol metabolic process Source: PomBase
    5. positive regulation of ascospore formation Source: PomBase
    6. protein localization to prospore membrane Source: PomBase
    7. protein localization to vacuole Source: PomBase
    8. vacuole organization Source: PomBase

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_188299. Synthesis of PIPs at the Golgi membrane.
    REACT_188300. Synthesis of PIPs at the early endosome membrane.
    REACT_188310. Synthesis of PIPs at the late endosome membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 3-kinase vps34 (EC:2.7.1.137)
    Short name:
    PI3-kinase vps34
    Short name:
    PI3K vps34
    Short name:
    PtdIns-3-kinase vps34
    Alternative name(s):
    Vacuolar protein sorting-associated protein 34
    Gene namesi
    Name:vps34
    ORF Names:SPAC458.05
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC458.05.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytosol Source: PomBase
    3. membrane Source: PomBase
    4. phosphatidylinositol 3-kinase complex Source: PomBase
    5. prospore membrane Source: PomBase
    6. vacuolar membrane Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 801801Phosphatidylinositol 3-kinase vps34PRO_0000088817Add
    BLAST

    Proteomic databases

    MaxQBiP50520.

    Interactioni

    Protein-protein interaction databases

    BioGridi279864. 3 interactions.
    MINTiMINT-4690623.
    STRINGi4896.SPAC458.05-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP50520.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 166153C2 PI3K-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini257 – 439183PIK helicalPROSITE-ProRule annotationAdd
    BLAST
    Domaini541 – 799259PI3K/PI4KPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
    Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
    Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
    Contains 1 PIK helical domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5032.
    HOGENOMiHOG000174003.
    KOiK00914.
    OMAiKISTHEW.
    OrthoDBiEOG7WHHK2.
    PhylomeDBiP50520.

    Family and domain databases

    Gene3Di1.10.1070.11. 1 hit.
    1.25.40.70. 1 hit.
    2.60.40.150. 1 hit.
    InterProiIPR016024. ARM-type_fold.
    IPR000008. C2_dom.
    IPR011009. Kinase-like_dom.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR018936. PI3/4_kinase_CS.
    IPR002420. PI3K_C2_dom.
    IPR008290. PI3K_Vps34.
    IPR015433. PI_Kinase.
    IPR001263. PInositide-3_kin_accessory_dom.
    [Graphical view]
    PANTHERiPTHR10048. PTHR10048. 1 hit.
    PfamiPF00454. PI3_PI4_kinase. 1 hit.
    PF00792. PI3K_C2. 1 hit.
    PF00613. PI3Ka. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000587. PI3K_Vps34. 1 hit.
    SMARTiSM00142. PI3K_C2. 1 hit.
    SM00145. PI3Ka. 1 hit.
    SM00146. PI3Kc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
    PS00916. PI3_4_KINASE_2. 1 hit.
    PS50290. PI3_4_KINASE_3. 1 hit.
    PS51547. PI3K_C2. 1 hit.
    PS51545. PIK_HELICAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50520-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDRLVFSYCP SSKVTARFLV KFCFIEYQDS QEPCICTIQL FSGNESGSLM    50
    QKCFVSKIPN KSLLPTELSK ISTHEWLDFG VTVSELSLNA KFVVSAWKPS 100
    FNDEEVYEFV GCTTYRLFDE NNLLRQGLQK IPLQTSKEIK KYSPTSLELE 150
    QVKEINRLDG LLLKLQLGDV PSVNWLDDIS FGKIKDFRSK HMSLVTIPIL 200
    YLDFLQFSFP VVFQRSYYPK SENRVYYSSF DLELNLDSPA ELKHRRLVRS 250
    QRNGPLDKDL KPNSKIRKEL ESILSYPPSE ELSLEEKDLI WKFRFYLTRN 300
    KKAMTKFLKS VVWTDSSEVN QALSLLDSWT EIDIDDALEL LSPSFVHPKV 350
    RAYAVSRLET ASNEELLLYL LQLVQALRYD NPISSDERFQ PSPLALFLVN 400
    RAISSPSIGN DLYWYLVVEI EDEPVSKLFS SVMFLFQKEL SKSVEGRLIR 450
    ETLSAQAKFV EKLLRISKSV QSFRGTRLKK IEYLKVLLED HKYHLLDFHA 500
    LPLPLDPSVN IVGIIPDACT VFKSTMQPLR LLFKCQDGSK YPIIFKNGDD 550
    LRQDQLVIQI LTLMDKLLKK EKLDLHLKPY RILATGPTHG AVQFVPSKTL 600
    ATILAEYHGS VLAYLRENNP DDGLNSANYG IDPVAMDNYV RSCAGYCVIT 650
    YLLGVGDRHL DNLLITKDGH FFHADFGYIL GRDPKLFSPA MKLSKEMVEG 700
    MGGYNSPFYQ QFKSYCYTTF TALRKSSNLI LNLFSLMVDA NIPDIKFDKE 750
    KVVYKVKERF CLQMSESDAI KYFEQLINDS VSALFPQIID RMHNLAQYMR 800
    S 801
    Length:801
    Mass (Da):92,135
    Last modified:August 14, 2001 - v2
    Checksum:i520571E1475CC341
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti164 – 1641K → Q in AAC49133. (PubMed:8719881)Curated
    Sequence conflicti236 – 2361L → I(PubMed:7772832)Curated
    Sequence conflicti642 – 6421S → T(PubMed:7772832)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32583 mRNA. Translation: AAC49133.1.
    CU329670 Genomic DNA. Translation: CAB93847.1.
    PIRiPC4002.
    T52538.
    RefSeqiNP_594699.1. NM_001020127.2.

    Genome annotation databases

    EnsemblFungiiSPAC458.05.1; SPAC458.05.1:pep; SPAC458.05.
    GeneIDi2543444.
    KEGGispo:SPAC458.05.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32583 mRNA. Translation: AAC49133.1 .
    CU329670 Genomic DNA. Translation: CAB93847.1 .
    PIRi PC4002.
    T52538.
    RefSeqi NP_594699.1. NM_001020127.2.

    3D structure databases

    ProteinModelPortali P50520.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279864. 3 interactions.
    MINTi MINT-4690623.
    STRINGi 4896.SPAC458.05-1.

    Proteomic databases

    MaxQBi P50520.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC458.05.1 ; SPAC458.05.1:pep ; SPAC458.05 .
    GeneIDi 2543444.
    KEGGi spo:SPAC458.05.

    Organism-specific databases

    PomBasei SPAC458.05.

    Phylogenomic databases

    eggNOGi COG5032.
    HOGENOMi HOG000174003.
    KOi K00914.
    OMAi KISTHEW.
    OrthoDBi EOG7WHHK2.
    PhylomeDBi P50520.

    Enzyme and pathway databases

    Reactomei REACT_188299. Synthesis of PIPs at the Golgi membrane.
    REACT_188300. Synthesis of PIPs at the early endosome membrane.
    REACT_188310. Synthesis of PIPs at the late endosome membrane.

    Miscellaneous databases

    NextBioi 20804457.
    PROi P50520.

    Family and domain databases

    Gene3Di 1.10.1070.11. 1 hit.
    1.25.40.70. 1 hit.
    2.60.40.150. 1 hit.
    InterProi IPR016024. ARM-type_fold.
    IPR000008. C2_dom.
    IPR011009. Kinase-like_dom.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR018936. PI3/4_kinase_CS.
    IPR002420. PI3K_C2_dom.
    IPR008290. PI3K_Vps34.
    IPR015433. PI_Kinase.
    IPR001263. PInositide-3_kin_accessory_dom.
    [Graphical view ]
    PANTHERi PTHR10048. PTHR10048. 1 hit.
    Pfami PF00454. PI3_PI4_kinase. 1 hit.
    PF00792. PI3K_C2. 1 hit.
    PF00613. PI3Ka. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000587. PI3K_Vps34. 1 hit.
    SMARTi SM00142. PI3K_C2. 1 hit.
    SM00145. PI3Ka. 1 hit.
    SM00146. PI3Kc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00915. PI3_4_KINASE_1. 1 hit.
    PS00916. PI3_4_KINASE_2. 1 hit.
    PS50290. PI3_4_KINASE_3. 1 hit.
    PS51547. PI3K_C2. 1 hit.
    PS51545. PIK_HELICAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Schizosaccharomyces pombe Vps34p, a phosphatidylinositol-specific PI 3-kinase essential for normal cell growth and vacuole morphology."
      Takegawa K., Dewald D.B., Emr S.E.
      J. Cell Sci. 108:3745-3756(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Phosphatidylinositol-3 kinase in fission yeast: a possible role in stress responses."
      Kimura K., Miyake S., Makuuchi M., Morita R., Usui T., Yoshida M., Horinouchi S., Fukui Y.
      Biosci. Biotechnol. Biochem. 59:678-682(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 138-801.

    Entry informationi

    Entry nameiVPS34_SCHPO
    AccessioniPrimary (citable) accession number: P50520
    Secondary accession number(s): Q9P3W3, Q9URD2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: August 14, 2001
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3