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P50520

- VPS34_SCHPO

UniProt

P50520 - VPS34_SCHPO

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Protein

Phosphatidylinositol 3-kinase vps34

Gene

vps34

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Phosphatidylinositol 3-kinase homolog required for vacuolar sorting and segregation.

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

GO - Molecular functioni

  1. 1-phosphatidylinositol-3-kinase activity Source: PomBase
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular protein localization Source: PomBase
  2. phosphatidylinositol-3-phosphate biosynthetic process Source: PomBase
  3. phosphatidylinositol-mediated signaling Source: InterPro
  4. phosphatidylinositol metabolic process Source: PomBase
  5. positive regulation of ascospore formation Source: PomBase
  6. protein localization to prospore membrane Source: PomBase
  7. protein localization to vacuole Source: PomBase
  8. vacuole organization Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_188299. Synthesis of PIPs at the Golgi membrane.
REACT_188300. Synthesis of PIPs at the early endosome membrane.
REACT_188310. Synthesis of PIPs at the late endosome membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase vps34 (EC:2.7.1.137)
Short name:
PI3-kinase vps34
Short name:
PI3K vps34
Short name:
PtdIns-3-kinase vps34
Alternative name(s):
Vacuolar protein sorting-associated protein 34
Gene namesi
Name:vps34
ORF Names:SPAC458.05
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC458.05.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. membrane Source: PomBase
  4. phosphatidylinositol 3-kinase complex Source: PomBase
  5. prospore membrane Source: PomBase
  6. vacuolar membrane Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 801801Phosphatidylinositol 3-kinase vps34PRO_0000088817Add
BLAST

Proteomic databases

MaxQBiP50520.

Interactioni

Protein-protein interaction databases

BioGridi279864. 3 interactions.
MINTiMINT-4690623.
STRINGi4896.SPAC458.05-1.

Structurei

3D structure databases

ProteinModelPortaliP50520.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 166153C2 PI3K-typePROSITE-ProRule annotationAdd
BLAST
Domaini257 – 439183PIK helicalPROSITE-ProRule annotationAdd
BLAST
Domaini541 – 799259PI3K/PI4KPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
Contains 1 PIK helical domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5032.
HOGENOMiHOG000174003.
InParanoidiP50520.
KOiK00914.
OMAiKISTHEW.
OrthoDBiEOG7WHHK2.
PhylomeDBiP50520.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR008290. PI3K_Vps34.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 1 hit.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
PIRSFiPIRSF000587. PI3K_Vps34. 1 hit.
SMARTiSM00142. PI3K_C2. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50520-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDRLVFSYCP SSKVTARFLV KFCFIEYQDS QEPCICTIQL FSGNESGSLM
60 70 80 90 100
QKCFVSKIPN KSLLPTELSK ISTHEWLDFG VTVSELSLNA KFVVSAWKPS
110 120 130 140 150
FNDEEVYEFV GCTTYRLFDE NNLLRQGLQK IPLQTSKEIK KYSPTSLELE
160 170 180 190 200
QVKEINRLDG LLLKLQLGDV PSVNWLDDIS FGKIKDFRSK HMSLVTIPIL
210 220 230 240 250
YLDFLQFSFP VVFQRSYYPK SENRVYYSSF DLELNLDSPA ELKHRRLVRS
260 270 280 290 300
QRNGPLDKDL KPNSKIRKEL ESILSYPPSE ELSLEEKDLI WKFRFYLTRN
310 320 330 340 350
KKAMTKFLKS VVWTDSSEVN QALSLLDSWT EIDIDDALEL LSPSFVHPKV
360 370 380 390 400
RAYAVSRLET ASNEELLLYL LQLVQALRYD NPISSDERFQ PSPLALFLVN
410 420 430 440 450
RAISSPSIGN DLYWYLVVEI EDEPVSKLFS SVMFLFQKEL SKSVEGRLIR
460 470 480 490 500
ETLSAQAKFV EKLLRISKSV QSFRGTRLKK IEYLKVLLED HKYHLLDFHA
510 520 530 540 550
LPLPLDPSVN IVGIIPDACT VFKSTMQPLR LLFKCQDGSK YPIIFKNGDD
560 570 580 590 600
LRQDQLVIQI LTLMDKLLKK EKLDLHLKPY RILATGPTHG AVQFVPSKTL
610 620 630 640 650
ATILAEYHGS VLAYLRENNP DDGLNSANYG IDPVAMDNYV RSCAGYCVIT
660 670 680 690 700
YLLGVGDRHL DNLLITKDGH FFHADFGYIL GRDPKLFSPA MKLSKEMVEG
710 720 730 740 750
MGGYNSPFYQ QFKSYCYTTF TALRKSSNLI LNLFSLMVDA NIPDIKFDKE
760 770 780 790 800
KVVYKVKERF CLQMSESDAI KYFEQLINDS VSALFPQIID RMHNLAQYMR

S
Length:801
Mass (Da):92,135
Last modified:August 14, 2001 - v2
Checksum:i520571E1475CC341
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1641K → Q in AAC49133. (PubMed:8719881)Curated
Sequence conflicti236 – 2361L → I(PubMed:7772832)Curated
Sequence conflicti642 – 6421S → T(PubMed:7772832)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U32583 mRNA. Translation: AAC49133.1.
CU329670 Genomic DNA. Translation: CAB93847.1.
PIRiPC4002.
T52538.
RefSeqiNP_594699.1. NM_001020127.2.

Genome annotation databases

EnsemblFungiiSPAC458.05.1; SPAC458.05.1:pep; SPAC458.05.
GeneIDi2543444.
KEGGispo:SPAC458.05.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U32583 mRNA. Translation: AAC49133.1 .
CU329670 Genomic DNA. Translation: CAB93847.1 .
PIRi PC4002.
T52538.
RefSeqi NP_594699.1. NM_001020127.2.

3D structure databases

ProteinModelPortali P50520.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 279864. 3 interactions.
MINTi MINT-4690623.
STRINGi 4896.SPAC458.05-1.

Proteomic databases

MaxQBi P50520.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC458.05.1 ; SPAC458.05.1:pep ; SPAC458.05 .
GeneIDi 2543444.
KEGGi spo:SPAC458.05.

Organism-specific databases

PomBasei SPAC458.05.

Phylogenomic databases

eggNOGi COG5032.
HOGENOMi HOG000174003.
InParanoidi P50520.
KOi K00914.
OMAi KISTHEW.
OrthoDBi EOG7WHHK2.
PhylomeDBi P50520.

Enzyme and pathway databases

Reactomei REACT_188299. Synthesis of PIPs at the Golgi membrane.
REACT_188300. Synthesis of PIPs at the early endosome membrane.
REACT_188310. Synthesis of PIPs at the late endosome membrane.

Miscellaneous databases

NextBioi 20804457.
PROi P50520.

Family and domain databases

Gene3Di 1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProi IPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR008290. PI3K_Vps34.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view ]
PANTHERi PTHR10048. PTHR10048. 1 hit.
Pfami PF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view ]
PIRSFi PIRSF000587. PI3K_Vps34. 1 hit.
SMARTi SM00142. PI3K_C2. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Schizosaccharomyces pombe Vps34p, a phosphatidylinositol-specific PI 3-kinase essential for normal cell growth and vacuole morphology."
    Takegawa K., Dewald D.B., Emr S.E.
    J. Cell Sci. 108:3745-3756(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Phosphatidylinositol-3 kinase in fission yeast: a possible role in stress responses."
    Kimura K., Miyake S., Makuuchi M., Morita R., Usui T., Yoshida M., Horinouchi S., Fukui Y.
    Biosci. Biotechnol. Biochem. 59:678-682(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 138-801.

Entry informationi

Entry nameiVPS34_SCHPO
AccessioniPrimary (citable) accession number: P50520
Secondary accession number(s): Q9P3W3, Q9URD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: August 14, 2001
Last modified: October 29, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3