P50520 (VPS34_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 3-kinase vps34
Short name=PI3-kinase vps34
Short name=PI3K vps34
Short name=PtdIns-3-kinase vps34
EC=2.7.1.137

Alternative name(s):
Vacuolar protein sorting-associated protein 34

Gene names

Name:	vps34
ORF Names:	SPAC458.05

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	801 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Phosphatidylinositol 3-kinase homolog required for vacuolar sorting and segregation.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 C2 PI3K-type domain. Contains 1 PI3K/PI4K domain. Contains 1 PIK helical domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ascospore wall assembly Inferred from mutant phenotype PubMed 12557273. Source: PomBase ascospore-type prospore assembly Inferred from mutant phenotype PubMed 12557273. Source: PomBase phosphatidylinositol-mediated signaling Inferred from electronic annotation. Source: InterPro positive regulation of ascospore formation Inferred from mutant phenotype PubMed 12557273. Source: PomBase positive regulation of protein targeting to prospore membrane Inferred from mutant phenotype PubMed 12557273. Source: PomBase positive regulation of protein targeting to vacuolar membrane Inferred from mutant phenotype PubMed 12557273. Source: PomBase protein targeting to vacuole Inferred from mutant phenotype PubMed 12557273. Source: PomBase vacuole organization Inferred from mutant phenotype Ref.1. Source: PomBase vesicle-mediated transport Traceable author statement PubMed 12557273. Source: PomBase
Cellular_component	membrane Inferred from mutant phenotype Ref.1. Source: PomBase phosphatidylinositol 3-kinase complex Inferred by curator. Source: PomBase
Molecular_function	1-phosphatidylinositol-3-kinase activity Inferred from direct assay Ref.1. Source: PomBase ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 801	801	Phosphatidylinositol 3-kinase vps34		PRO_0000088817
Regions
	Domain	14 – 166	153	C2 PI3K-type
	Domain	257 – 439	183	PIK helical
	Domain	541 – 799	259	PI3K/PI4K
Experimental info
	Sequence conflict	164	1	K → Q in AAC49133. Ref.1
	Sequence conflict	236	1	L → I Ref.3
	Sequence conflict	642	1	S → T Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P50520 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: 520571E1475CC341	FASTA	801	92,135
	10 20 30 40 50 60 MDRLVFSYCP SSKVTARFLV KFCFIEYQDS QEPCICTIQL FSGNESGSLM QKCFVSKIPN 70 80 90 100 110 120 KSLLPTELSK ISTHEWLDFG VTVSELSLNA KFVVSAWKPS FNDEEVYEFV GCTTYRLFDE 130 140 150 160 170 180 NNLLRQGLQK IPLQTSKEIK KYSPTSLELE QVKEINRLDG LLLKLQLGDV PSVNWLDDIS 190 200 210 220 230 240 FGKIKDFRSK HMSLVTIPIL YLDFLQFSFP VVFQRSYYPK SENRVYYSSF DLELNLDSPA 250 260 270 280 290 300 ELKHRRLVRS QRNGPLDKDL KPNSKIRKEL ESILSYPPSE ELSLEEKDLI WKFRFYLTRN 310 320 330 340 350 360 KKAMTKFLKS VVWTDSSEVN QALSLLDSWT EIDIDDALEL LSPSFVHPKV RAYAVSRLET 370 380 390 400 410 420 ASNEELLLYL LQLVQALRYD NPISSDERFQ PSPLALFLVN RAISSPSIGN DLYWYLVVEI 430 440 450 460 470 480 EDEPVSKLFS SVMFLFQKEL SKSVEGRLIR ETLSAQAKFV EKLLRISKSV QSFRGTRLKK 490 500 510 520 530 540 IEYLKVLLED HKYHLLDFHA LPLPLDPSVN IVGIIPDACT VFKSTMQPLR LLFKCQDGSK 550 560 570 580 590 600 YPIIFKNGDD LRQDQLVIQI LTLMDKLLKK EKLDLHLKPY RILATGPTHG AVQFVPSKTL 610 620 630 640 650 660 ATILAEYHGS VLAYLRENNP DDGLNSANYG IDPVAMDNYV RSCAGYCVIT YLLGVGDRHL 670 680 690 700 710 720 DNLLITKDGH FFHADFGYIL GRDPKLFSPA MKLSKEMVEG MGGYNSPFYQ QFKSYCYTTF 730 740 750 760 770 780 TALRKSSNLI LNLFSLMVDA NIPDIKFDKE KVVYKVKERF CLQMSESDAI KYFEQLINDS 790 800 VSALFPQIID RMHNLAQYMR S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Schizosaccharomyces pombe Vps34p, a phosphatidylinositol-specific PI 3-kinase essential for normal cell growth and vacuole morphology." Takegawa K., Dewald D.B., Emr S.E. J. Cell Sci. 108:3745-3756(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Phosphatidylinositol-3 kinase in fission yeast: a possible role in stress responses." Kimura K., Miyake S., Makuuchi M., Morita R., Usui T., Yoshida M., Horinouchi S., Fukui Y. Biosci. Biotechnol. Biochem. 59:678-682(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 138-801.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U32583 mRNA. Translation: AAC49133.1. CU329670 Genomic DNA. Translation: CAB93847.1.
PIR	PC4002. T52538.
RefSeq	NP_594699.1. NM_001020127.2.
3D structure databases
ProteinModelPortal	P50520.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAC458.05-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC458.05.1; SPAC458.05.1:pep; SPAC458.05.
GeneID	2543444.
KEGG	spo:SPAC458.05.
Organism-specific databases
PomBase	SPAC458.05.
Phylogenomic databases
eggNOG	COG5032.
HOGENOM	HOG000174003.
KO	K00914.
OMA	FRERRED.
OrthoDB	EOG4PP1R1.
Family and domain databases
Gene3D	1.10.1070.11. 1 hit. 1.25.40.70. 1 hit.
InterPro	IPR016024. ARM-type_fold. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat_dom. IPR018936. PI3/4_kinase_CS. IPR002420. PI3K_C2_dom. IPR008290. PI3K_Vps34. IPR015433. PI_Kinase. IPR001263. PInositide-3_kin_accessory_dom. [Graphical view]
PANTHER	PTHR10048. PTHR10048. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF00792. PI3K_C2. 1 hit. PF00613. PI3Ka. 1 hit. [Graphical view]
PIRSF	PIRSF000587. PI3K_Vps34. 1 hit.
SMART	SM00142. PI3K_C2. 1 hit. SM00145. PI3Ka. 1 hit. SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF49562. C2_CaLB. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. PS51547. PI3K_C2. 1 hit. PS51545. PIK_HELICAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20804457.

Entry information

Entry name

VPS34_SCHPO

Accession

Primary (citable) accession number: P50520
Secondary accession number(s): Q9P3W3, Q9URD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	August 14, 2001
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program