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Protein

V-type proton ATPase catalytic subunit A

Gene

Atp6v1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (By similarity).By similarity

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi250 – 257ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processHydrogen ion transport, Ion transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1222556 ROS, RNS production in phagocytes
R-MMU-77387 Insulin receptor recycling
R-MMU-917977 Transferrin endocytosis and recycling
R-MMU-983712 Ion channel transport

Protein family/group databases

TCDBi3.A.2.2.6 the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase catalytic subunit A (EC:3.6.3.14)
Short name:
V-ATPase subunit A
Alternative name(s):
V-ATPase 69 kDa subunit
Vacuolar proton pump subunit alpha
Gene namesi
Name:Atp6v1a
Synonyms:Atp6a1, Atp6a2, Atp6v1a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1201780 Atp6v1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001445611 – 617V-type proton ATPase catalytic subunit AAdd BLAST617

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei384PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP50516
MaxQBiP50516
PaxDbiP50516
PeptideAtlasiP50516
PRIDEiP50516
TopDownProteomicsiP50516-1 [P50516-1]

PTM databases

iPTMnetiP50516
PhosphoSitePlusiP50516
SwissPalmiP50516

Expressioni

Gene expression databases

BgeeiENSMUSG00000052459
CleanExiMM_ATP6V1A
ExpressionAtlasiP50516 baseline and differential
GenevisibleiP50516 MM

Interactioni

Subunit structurei

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein).

Protein-protein interaction databases

BioGridi198261, 9 interactors
IntActiP50516, 108 interactors
MINTiP50516
STRINGi10090.ENSMUSP00000066886

Structurei

3D structure databases

ProteinModelPortaliP50516
SMRiP50516
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.Curated

Phylogenomic databases

eggNOGiKOG1352 Eukaryota
COG1155 LUCA
GeneTreeiENSGT00550000074787
HOVERGENiHBG053351
InParanoidiP50516
KOiK02145
OMAiQQLAKWS
OrthoDBiEOG091G03SR
PhylomeDBiP50516
TreeFamiTF300811

Family and domain databases

Gene3Di1.10.1140.10, 1 hit
2.40.30.20, 1 hit
HAMAPiMF_00309 ATP_synth_A_arch, 1 hit
InterProiView protein in InterPro
IPR031686 ATP-synth_a_Xtn
IPR023366 ATP_synth_asu-like_sf
IPR020003 ATPase_a/bsu_AS
IPR004100 ATPase_F1/V1/A1_a/bsu_N
IPR036121 ATPase_F1/V1/A1_a/bsu_N_sf
IPR000194 ATPase_F1/V1/A1_a/bsu_nucl-bd
IPR024034 ATPase_F1/V1_b/a_C
IPR005725 ATPase_V1-cplx_asu
IPR027417 P-loop_NTPase
IPR022878 V-ATPase_asu
PANTHERiPTHR43607 PTHR43607, 1 hit
PfamiView protein in Pfam
PF00006 ATP-synt_ab, 1 hit
PF02874 ATP-synt_ab_N, 1 hit
PF16886 ATP-synt_ab_Xtn, 1 hit
SUPFAMiSSF50615 SSF50615, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01042 V-ATPase_V1_A, 1 hit
PROSITEiView protein in PROSITE
PS00152 ATPASE_ALPHA_BETA, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P50516-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDFSKLPKIR DEDKESTFGY VHGVSGPVVT ACDMAGAAMY ELVRVGHSEL
60 70 80 90 100
VGEIIRLEGD MATIQVYEET SGVSVGDPVL RTGKPLSVEL GPGIMGAIFD
110 120 130 140 150
GIQRPLSDIS SQTQSIYIPR GVNVSALSRD IKWEFIPSKN LRVGSHITGG
160 170 180 190 200
DIYGIVNENS LIKHKIMLPP RNRGSVTYIA PPGNYDASDV VLELEFEGVK
210 220 230 240 250
EKFSMVQVWP VRQVRPVTEK LPANHPLLTG QRVLDALFPC VQGGTTAIPG
260 270 280 290 300
AFGCGKTVIS QSLSKYSNSD VIIYVGCGER GNEMSEVLRD FPELTMEVDG
310 320 330 340 350
KVESIMKRTA LVANTSNMPV AAREASIYTG ITLSEYFRDM GYHVSMMADS
360 370 380 390 400
TSRWAEALRE ISGRLAEMPA DSGYPAYLGA RLASFYERAG RVKCLGNPER
410 420 430 440 450
EGSVSIVGAV SPPGGDFSDP VTSATLGIVQ VFWGLDKKLA QRKHFPSVNW
460 470 480 490 500
LISYSKYMRA LDEYYDKHFT EFVPLRTKAK EILQEEEDLA EIVQLVGKAS
510 520 530 540 550
LAETDKITLE VAKLIKDDFL QQNGYTPYDR FCPFYKTVGM LSNMISFYDM
560 570 580 590 600
ARRAVETTAQ SDNKITWSII REHMGEILYK LSSMKFKDPV KDGEAKIKAD
610
YAQLLEDMQN AFRSLED
Length:617
Mass (Da):68,326
Last modified:April 17, 2007 - v2
Checksum:iD46AC76D9C7580A7
GO
Isoform 2 (identifier: P50516-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     499-509: ASLAETDKITL → VRGGCTGCHAG
     510-617: Missing.

Note: No experimental confirmation available.
Show »
Length:509
Mass (Da):55,595
Checksum:i55D9F7CCEF00CC89
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86L → R in AAC52410 (PubMed:8741845).Curated1
Sequence conflicti189D → N in AAC52410 (PubMed:8741845).Curated1
Sequence conflicti302V → A in AAC52410 (PubMed:8741845).Curated1
Sequence conflicti330G → V in BAE39074 (PubMed:16141072).Curated1
Sequence conflicti487E → G in AAC52410 (PubMed:8741845).Curated1
Sequence conflicti616E → G in BAE31963 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024628499 – 509ASLAETDKITL → VRGGCTGCHAG in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_024629510 – 617Missing in isoform 2. 1 PublicationAdd BLAST108

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13837 Genomic DNA Translation: AAC52410.1
AK140873 mRNA Translation: BAE24506.1
AK149833 mRNA Translation: BAE29112.1
AK152785 mRNA Translation: BAE31494.1
AK153403 mRNA Translation: BAE31963.1
AK154869 mRNA Translation: BAE32890.1
AK160792 mRNA Translation: BAE36015.1
AK166857 mRNA Translation: BAE39074.1
AK170721 mRNA Translation: BAE41978.1
BC038392 mRNA Translation: AAH38392.1
CCDSiCCDS28182.1 [P50516-1]
RefSeqiNP_031534.2, NM_007508.5 [P50516-1]
XP_006521783.1, XM_006521720.3
XP_006521784.1, XM_006521721.2 [P50516-1]
XP_011244111.1, XM_011245809.2
UniGeneiMm.217787

Genome annotation databases

EnsembliENSMUST00000063661; ENSMUSP00000066886; ENSMUSG00000052459 [P50516-1]
ENSMUST00000114666; ENSMUSP00000110314; ENSMUSG00000052459 [P50516-1]
GeneIDi11964
KEGGimmu:11964
UCSCiuc007zgu.1 mouse [P50516-1]
uc007zgw.1 mouse [P50516-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVATA_MOUSE
AccessioniPrimary (citable) accession number: P50516
Secondary accession number(s): Q3TKS0
, Q3U5W3, Q3U777, Q3UDZ9, Q3US31, Q8CHX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 17, 2007
Last modified: May 23, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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