Cell surface receptor daf-4 precursor - Caenorhabditis elegans

Contribute

Send feedback

Reviewed, UniProtKB/Swiss-Prot P50488 (DAF4_CAEEL)

Last modified November 25, 2008. Version 75. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Cell surface receptor daf-4
    EC=2.7.11.30
Alternative name(s):
    Abnormal dauer formation protein 4

Gene names

Name:	daf-4
ORF Names:	C05D2.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Hide | Top

Protein attributes

Sequence length	744 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	Involved in TGF-beta pathway. May be a receptor for daf-7. Controls the dauer/nondauer developmental decision in a range of environmental conditions. Regulates body size and male tail patterning.
Catalytic activity	ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.
Subunit structure	May interact with daf-1 to regulate dauer larva development.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Pharynx, intestine, hypodermis and body wall muscles in L1 through to adult stages. Also expressed in head neurons, ventral cord and tail neurons. Subset of head neurons show coexpression with daf-1 when dauer/nondauer decision is made.
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. Contains 1 protein kinase domain.

Hide | Top

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dauer larval development Ref.1 Ref.3 Inferred from mutant phenotype. Source: WormBase negative regulation of BMP signaling pathway Inferred from mutant phenotype. Source: WormBase oviposition Inferred from mutant phenotype. Source: WormBase positive regulation of BMP signaling pathway Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase positive regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct receptor activity Inferred from electronic annotation. Source: UniProtKB-KW receptor signaling protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Hide | Top

Binary interactions

With	Entry	#Exp.	IntAct	Notes
atp-2	P46561	1	EBI-296172,EBI-316294
scrm-1	O45799	1	EBI-296172,EBI-312683

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 31

Potential

Chain

32 – 744

713

Cell surface receptor daf-4

PRO_0000024431

Regions

Topological domain

48 – 253

206

Extracellular Potential

Transmembrane

254 – 274

Potential

Topological domain

275 – 744

470

Cytoplasmic Potential

Domain

306 – 603

298

Protein kinase

Nucleotide binding

312 – 320

ATP By similarity

Sites

Active site

440

Proton acceptor By similarity

Binding site

338

ATP By similarity

Amino acid modifications

Glycosylation

N-linked (GlcNAc...)

Glycosylation

134

N-linked (GlcNAc...)

Glycosylation

165

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

374

A → P in AAA03544. Ref.1

Sequence conflict

545

L → R in AAA03544. Ref.1

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

P50488-1 [UniParc].

Last modified July 3, 2003. Version 2.
Checksum: 33C087871FD81AF0
Show »

FASTA

744

84,410

        10         20         30         40         50         60 
MNQKGTVRLK ALVLICLPLF LIATPVPVAV TEDDRQDRIE SEAAEKEWAN TLVSKVAQSN 

        70         80         90        100        110        120 
GTGTIKVSAP AKPTLRRMDN EEDEVISIEC VYYDEMECEK SGDCEITKKT CYSEAHLKAV 

       130        140        150        160        170        180 
GCLAVFGLPT QEINSTEPYL KVDKPQYKSL GCMPYQHADS MNCENESSCR QGRSFRGGIG 

       190        200        210        220        230        240 
MCCCSTNNCN MPDLIEMVNP SLKKDSDNSA LLWASTPSNM DLESLDKFPF YWIIIIALSV 

       250        260        270        280        290        300 
ILCIALLILA YVGWKFQQNK KEEIKKQQKI KFDMEKTDAL EAGNVPLVEP EEEMIEMVET 

       310        320        330        340        350        360 
PKELPITDFQ LISKGRFGKV FKAQYTPDSG EKRLVAVKKL NEFQKASFLA EKRIFDELNE 

       370        380        390        400        410        420 
YPKWYKSIVE FVCAEKIGDE YWIVTEFHER LSLYELLKNN VISITSANRI IMSMIDGLQF 

       430        440        450        460        470        480 
LHDDRPYFFG HPKKPIIHRD IKSKNILVKS DMTTCIADFG LARIYSYDIE QSDLLGQVGT 

       490        500        510        520        530        540 
KRYMSPEMLE GATEFTPTAF KAMDVYSMGL VMWEVISRTK LHQTDEPPNY QMPFQVIGFD 

       550        560        570        580        590        600 
PTIGLMRNYV VSKKERPQWR DEIIKHEYMS LLKKVTEEMW DPEACARITA GCAFARVWNH 

       610        620        630        640        650        660 
IMSSPDSSEG YHSGSSMKNR GVDDVEQSEK PEGIEEMQHY HASSPSKRQH PSPNPFFDSC 

       670        680        690        700        710        720 
PPPPPIPVIL ENGGILQPDN AEPEPEELPD LPIVEKIYDI ATNMLFSREE LDLMNAQRQV 

       730        740 
EYEAGADTRA STPTPSGTFG TFTT

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The daf-4 gene encodes a bone morphogenetic protein receptor controlling C. elegans dauer larva development." Estevez M., Attisano L., Wrana J.L., Albert P.S., Massague J., Riddle D.L. Nature 365:644-649(1993) [PubMed: 8413626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"A Caenorhabditis elegans type I TGF beta receptor can function in the absence of type II kinase to promote larval development." Gunther C.V., Georgi L.L., Riddle D.L. Development 127:3337-3347(2000) [PubMed: 10887089] [Abstract] Cited for: FUNCTION, SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[4]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-60 AND ASN-134, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases
	L23110 mRNA. Translation: AAA03544.1. AF047651 Genomic DNA. Translation: AAC02726.1.
PIR	D88462. S38279.
RefSeq	NP_498211.1.
UniGene	Cel.6349
3D structure databases
HSSP	HSSP built from PDB template 1IAS based on UniProtKB P36897.
ModBase	Search...
Protein-protein interaction databases
IntAct	P50488.
Genome annotation databases
Ensembl	C05D2.1. Caenorhabditis elegans. [Contig view]
GeneID	175781.
Organism-specific databases
WormBase	WBGene00000900. daf-4.
WormPep	C05D2.1a. CE16827. [WorfDB]
Gene expression databases
ArrayExpress	P50488.
Family and domain databases
InterPro	IPR000333. Activin_II_recpt. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR00653. ACTIVIN2R.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	889638.

Hide | Top

Entry information

Entry name

DAF4_CAEEL

Accession

Primary (citable) accession number: P50488
Secondary accession number(s): O45139

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 3, 2003
Last modified:	November 25, 2008
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project

Hide | Top

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents