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P50488

- DAF4_CAEEL

UniProt

P50488 - DAF4_CAEEL

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Protein

Cell surface receptor daf-4

Gene

daf-4

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in TGF-beta pathway. May be a receptor for daf-7. Controls the dauer/nondauer developmental decision in a range of environmental conditions. Regulates body size and male tail patterning.1 Publication

Catalytic activityi

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei338 – 3381ATPPROSITE-ProRule annotation
Active sitei440 – 4401Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi312 – 3209ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. BMP binding Source: WormBase
  3. receptor signaling protein serine/threonine kinase activity Source: InterPro
  4. transmembrane receptor protein serine/threonine kinase activity Source: WormBase

GO - Biological processi

  1. BMP signaling pathway Source: WormBase
  2. dauer larval development Source: WormBase
  3. defense response to fungus Source: WormBase
  4. innate immune response Source: WormBase
  5. negative regulation of BMP signaling pathway Source: WormBase
  6. nematode larval development Source: WormBase
  7. oviposition Source: WormBase
  8. positive regulation of BMP signaling pathway Source: WormBase
  9. positive regulation of multicellular organism growth Source: WormBase
  10. positive regulation of transcription from RNA polymerase II promoter Source: WormBase
  11. regulation of cell morphogenesis Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 1045.
ReactomeiREACT_107985. Signaling by BMP.
REACT_183486. Signaling by NODAL.
SignaLinkiP50488.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell surface receptor daf-4 (EC:2.7.11.30)
Alternative name(s):
Abnormal dauer formation protein 4
Gene namesi
Name:daf-4
ORF Names:C05D2.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiC05D2.1a; CE16827; WBGene00000900; daf-4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini48 – 253206ExtracellularSequence AnalysisAdd
BLAST
Transmembranei254 – 27421HelicalSequence AnalysisAdd
BLAST
Topological domaini275 – 744470CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 744713Cell surface receptor daf-4PRO_0000024431Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi60 – 601N-linked (GlcNAc...)1 Publication
Glycosylationi134 – 1341N-linked (GlcNAc...)1 Publication
Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP50488.
PRIDEiP50488.

Expressioni

Tissue specificityi

Pharynx, intestine, hypodermis and body wall muscles in L1 through to adult stages. Also expressed in head neurons, ventral cord and tail neurons. Subset of head neurons show coexpression with daf-1 when dauer/nondauer decision is made.1 Publication

Gene expression databases

ExpressionAtlasiP50488. baseline.

Interactioni

Subunit structurei

May interact with daf-1 to regulate dauer larva development.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
atp-2P465612EBI-296172,EBI-316294
scrm-1O457992EBI-296172,EBI-312683

Protein-protein interaction databases

BioGridi41009. 15 interactions.
IntActiP50488. 14 interactions.
MINTiMINT-206636.

Structurei

3D structure databases

ProteinModelPortaliP50488.
SMRiP50488. Positions 296-597.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini306 – 603298Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00730000111964.
HOGENOMiHOG000018794.
InParanoidiP50488.
KOiK13597.
OMAiKAMDVYS.
OrthoDBiEOG7JHM5B.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR000333. TGFB_receptor.
[Graphical view]
PANTHERiPTHR23255. PTHR23255. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50488-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNQKGTVRLK ALVLICLPLF LIATPVPVAV TEDDRQDRIE SEAAEKEWAN
60 70 80 90 100
TLVSKVAQSN GTGTIKVSAP AKPTLRRMDN EEDEVISIEC VYYDEMECEK
110 120 130 140 150
SGDCEITKKT CYSEAHLKAV GCLAVFGLPT QEINSTEPYL KVDKPQYKSL
160 170 180 190 200
GCMPYQHADS MNCENESSCR QGRSFRGGIG MCCCSTNNCN MPDLIEMVNP
210 220 230 240 250
SLKKDSDNSA LLWASTPSNM DLESLDKFPF YWIIIIALSV ILCIALLILA
260 270 280 290 300
YVGWKFQQNK KEEIKKQQKI KFDMEKTDAL EAGNVPLVEP EEEMIEMVET
310 320 330 340 350
PKELPITDFQ LISKGRFGKV FKAQYTPDSG EKRLVAVKKL NEFQKASFLA
360 370 380 390 400
EKRIFDELNE YPKWYKSIVE FVCAEKIGDE YWIVTEFHER LSLYELLKNN
410 420 430 440 450
VISITSANRI IMSMIDGLQF LHDDRPYFFG HPKKPIIHRD IKSKNILVKS
460 470 480 490 500
DMTTCIADFG LARIYSYDIE QSDLLGQVGT KRYMSPEMLE GATEFTPTAF
510 520 530 540 550
KAMDVYSMGL VMWEVISRTK LHQTDEPPNY QMPFQVIGFD PTIGLMRNYV
560 570 580 590 600
VSKKERPQWR DEIIKHEYMS LLKKVTEEMW DPEACARITA GCAFARVWNH
610 620 630 640 650
IMSSPDSSEG YHSGSSMKNR GVDDVEQSEK PEGIEEMQHY HASSPSKRQH
660 670 680 690 700
PSPNPFFDSC PPPPPIPVIL ENGGILQPDN AEPEPEELPD LPIVEKIYDI
710 720 730 740
ATNMLFSREE LDLMNAQRQV EYEAGADTRA STPTPSGTFG TFTT
Length:744
Mass (Da):84,410
Last modified:July 3, 2003 - v2
Checksum:i33C087871FD81AF0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti374 – 3741A → P in AAA03544. (PubMed:8413626)Curated
Sequence conflicti545 – 5451L → R in AAA03544. (PubMed:8413626)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23110 mRNA. Translation: AAA03544.1.
FO080356 Genomic DNA. Translation: CCD63118.1.
PIRiD88462.
S38279.
RefSeqiNP_498211.1. NM_065810.5.
UniGeneiCel.6349.

Genome annotation databases

EnsemblMetazoaiC05D2.1a; C05D2.1a; WBGene00000900.
GeneIDi175781.
KEGGicel:CELE_C05D2.1.
UCSCiC05D2.1a.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23110 mRNA. Translation: AAA03544.1 .
FO080356 Genomic DNA. Translation: CCD63118.1 .
PIRi D88462.
S38279.
RefSeqi NP_498211.1. NM_065810.5.
UniGenei Cel.6349.

3D structure databases

ProteinModelPortali P50488.
SMRi P50488. Positions 296-597.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 41009. 15 interactions.
IntActi P50488. 14 interactions.
MINTi MINT-206636.

Proteomic databases

PaxDbi P50488.
PRIDEi P50488.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai C05D2.1a ; C05D2.1a ; WBGene00000900 .
GeneIDi 175781.
KEGGi cel:CELE_C05D2.1.
UCSCi C05D2.1a.1. c. elegans.

Organism-specific databases

CTDi 175781.
WormBasei C05D2.1a ; CE16827 ; WBGene00000900 ; daf-4.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00730000111964.
HOGENOMi HOG000018794.
InParanoidi P50488.
KOi K13597.
OMAi KAMDVYS.
OrthoDBi EOG7JHM5B.

Enzyme and pathway databases

BRENDAi 2.7.10.2. 1045.
Reactomei REACT_107985. Signaling by BMP.
REACT_183486. Signaling by NODAL.
SignaLinki P50488.

Miscellaneous databases

NextBioi 889638.

Gene expression databases

ExpressionAtlasi P50488. baseline.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR000333. TGFB_receptor.
[Graphical view ]
PANTHERi PTHR23255. PTHR23255. 1 hit.
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The daf-4 gene encodes a bone morphogenetic protein receptor controlling C. elegans dauer larva development."
    Estevez M., Attisano L., Wrana J.L., Albert P.S., Massague J., Riddle D.L.
    Nature 365:644-649(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "A Caenorhabditis elegans type I TGF beta receptor can function in the absence of type II kinase to promote larval development."
    Gunther C.V., Georgi L.L., Riddle D.L.
    Development 127:3337-3347(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  4. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-60 AND ASN-134, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiDAF4_CAEEL
AccessioniPrimary (citable) accession number: P50488
Secondary accession number(s): O45139
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 3, 2003
Last modified: November 26, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3