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Reviewed, UniProtKB/Swiss-Prot P50488 (DAF4_CAEEL)

Last modified January 19, 2010. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cell surface receptor daf-4
    EC=2.7.11.30
Alternative name(s):
    Abnormal dauer formation protein 4
Gene names
Name: daf-4
ORF Names: C05D2.1
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length744 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Involved in TGF-beta pathway. May be a receptor for daf-7. Controls the dauer/nondauer developmental decision in a range of environmental conditions. Regulates body size and male tail patterning. Ref.3

Catalytic activity

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Subunit structure

May interact with daf-1 to regulate dauer larva development. Ref.3

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Pharynx, intestine, hypodermis and body wall muscles in L1 through to adult stages. Also expressed in head neurons, ventral cord and tail neurons. Subset of head neurons show coexpression with daf-1 when dauer/nondauer decision is made. Ref.3

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

atp-2P465611EBI-296172,EBI-316294
scrm-1O457991EBI-296172,EBI-312683

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 744713Cell surface receptor daf-4
PRO_0000024431

Regions

Topological domain48 – 253206Extracellular Potential
Transmembrane254 – 27421 Potential
Topological domain275 – 744470Cytoplasmic Potential
Domain306 – 603298Protein kinase
Nucleotide binding312 – 3209ATP By similarity

Sites

Active site4401Proton acceptor By similarity
Binding site3381ATP By similarity

Amino acid modifications

Glycosylation601N-linked (GlcNAc...) Ref.4
Glycosylation1341N-linked (GlcNAc...) Ref.4
Glycosylation1651N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict3741A → P in AAA03544. Ref.1
Sequence conflict5451L → R in AAA03544. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P50488-1 [UniParc].

Last modified July 3, 2003. Version 2.
Checksum: 33C087871FD81AF0

FASTA74484,410
        10         20         30         40         50         60 
MNQKGTVRLK ALVLICLPLF LIATPVPVAV TEDDRQDRIE SEAAEKEWAN TLVSKVAQSN 

        70         80         90        100        110        120 
GTGTIKVSAP AKPTLRRMDN EEDEVISIEC VYYDEMECEK SGDCEITKKT CYSEAHLKAV 

       130        140        150        160        170        180 
GCLAVFGLPT QEINSTEPYL KVDKPQYKSL GCMPYQHADS MNCENESSCR QGRSFRGGIG 

       190        200        210        220        230        240 
MCCCSTNNCN MPDLIEMVNP SLKKDSDNSA LLWASTPSNM DLESLDKFPF YWIIIIALSV 

       250        260        270        280        290        300 
ILCIALLILA YVGWKFQQNK KEEIKKQQKI KFDMEKTDAL EAGNVPLVEP EEEMIEMVET 

       310        320        330        340        350        360 
PKELPITDFQ LISKGRFGKV FKAQYTPDSG EKRLVAVKKL NEFQKASFLA EKRIFDELNE 

       370        380        390        400        410        420 
YPKWYKSIVE FVCAEKIGDE YWIVTEFHER LSLYELLKNN VISITSANRI IMSMIDGLQF 

       430        440        450        460        470        480 
LHDDRPYFFG HPKKPIIHRD IKSKNILVKS DMTTCIADFG LARIYSYDIE QSDLLGQVGT 

       490        500        510        520        530        540 
KRYMSPEMLE GATEFTPTAF KAMDVYSMGL VMWEVISRTK LHQTDEPPNY QMPFQVIGFD 

       550        560        570        580        590        600 
PTIGLMRNYV VSKKERPQWR DEIIKHEYMS LLKKVTEEMW DPEACARITA GCAFARVWNH 

       610        620        630        640        650        660 
IMSSPDSSEG YHSGSSMKNR GVDDVEQSEK PEGIEEMQHY HASSPSKRQH PSPNPFFDSC 

       670        680        690        700        710        720 
PPPPPIPVIL ENGGILQPDN AEPEPEELPD LPIVEKIYDI ATNMLFSREE LDLMNAQRQV 

       730        740 
EYEAGADTRA STPTPSGTFG TFTT 

« Hide

References

« Hide 'large scale' references
[1]"The daf-4 gene encodes a bone morphogenetic protein receptor controlling C. elegans dauer larva development."
Estevez M., Attisano L., Wrana J.L., Albert P.S., Massague J., Riddle D.L.
Nature 365:644-649(1993) [PubMed: 8413626] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[3]"A Caenorhabditis elegans type I TGF beta receptor can function in the absence of type II kinase to promote larval development."
Gunther C.V., Georgi L.L., Riddle D.L.
Development 127:3337-3347(2000) [PubMed: 10887089] [Abstract]
Cited for: FUNCTION, SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[4]"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-60 AND ASN-134, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L23110 mRNA. Translation: AAA03544.1.
AF047651 Genomic DNA. Translation: AAC02726.1.
PIRD88462.
S38279.
RefSeqNP_498211.1.
UniGeneCel.6349

3D structure databases

SMRP50488. Positions 309-601.
ModBaseSearch...

Protein-protein interaction databases

IntActP50488. 14 interactions.
STRINGP50488.

Proteomic databases

PRIDEP50488.

Genome annotation databases

EnsemblC05D2.1a.1; C05D2.1a.1; C05D2.1; Caenorhabditis elegans. [Genome view]
GeneID175781.
UCSCC05D2.1a.1. c. elegans.

Organism-specific databases

CTD175781.
WormBaseWBGene00000900. daf-4.
WormPepC05D2.1a. CE16827. [WorfDB]

Phylogenomic databases

eggNOGmeNOG06987.
HOGENOMHBG382768.
InParanoidP50488.
OMAEMVETPK.
PhylomeDBP50488.

Enzyme and pathway databases

BRENDA2.7.10.2. 672.
2.7.11.30. 672.

Gene expression databases

ArrayExpressP50488.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017442. Se/Thr_prot_kinase-like_dom.
IPR008271. Ser/Thr_prot_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio889638.

Entry information

Entry nameDAF4_CAEEL
AccessionPrimary (citable) accession number: P50488
Secondary accession number(s): O45139
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 3, 2003
Last modified: January 19, 2010
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents