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P50488 (DAF4_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell surface receptor daf-4

EC=2.7.11.30
Alternative name(s):
Abnormal dauer formation protein 4
Gene names
Name:daf-4
ORF Names:C05D2.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length744 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in TGF-beta pathway. May be a receptor for daf-7. Controls the dauer/nondauer developmental decision in a range of environmental conditions. Regulates body size and male tail patterning. Ref.3

Catalytic activity

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Subunit structure

May interact with daf-1 to regulate dauer larva development. Ref.3

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Pharynx, intestine, hypodermis and body wall muscles in L1 through to adult stages. Also expressed in head neurons, ventral cord and tail neurons. Subset of head neurons show coexpression with daf-1 when dauer/nondauer decision is made. Ref.3

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Cellular componentMembrane
   DomainSignal
Transmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   Molecular functionDevelopmental protein
Kinase
Receptor
Serine/threonine-protein kinase
Transferase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processBMP signaling pathway

Inferred by curator Ref.1. Source: WormBase

dauer larval development

Inferred from mutant phenotype PubMed 10625546Ref.3PubMed 12717735PubMed 15254038Ref.1. Source: WormBase

defense response to fungus

Inferred from mutant phenotype PubMed 19198592. Source: WormBase

innate immune response

Inferred from mutant phenotype PubMed 19198592. Source: WormBase

negative regulation of BMP signaling pathway

Inferred from mutant phenotype PubMed 15254038. Source: WormBase

nematode larval development

Inferred from mutant phenotype PubMed 15254038. Source: WormBase

oviposition

Inferred from mutant phenotype PubMed 10625546. Source: WormBase

positive regulation of BMP signaling pathway

Inferred from mutant phenotype PubMed 15254038. Source: WormBase

positive regulation of multicellular organism growth

Inferred from mutant phenotype PubMed 10625546PubMed 12717735. Source: WormBase

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 19198592PubMed 9834189. Source: WormBase

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from direct assay PubMed 9334330. Source: WormBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

BMP binding

Inferred from physical interaction Ref.1. Source: WormBase

transmembrane receptor protein serine/threonine kinase activity

Inferred from sequence or structural similarity Ref.1. Source: WormBase

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

atp-2P465612EBI-296172,EBI-316294
scrm-1O457992EBI-296172,EBI-312683

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 744713Cell surface receptor daf-4
PRO_0000024431

Regions

Topological domain48 – 253206Extracellular Potential
Transmembrane254 – 27421Helical; Potential
Topological domain275 – 744470Cytoplasmic Potential
Domain306 – 603298Protein kinase
Nucleotide binding312 – 3209ATP By similarity

Sites

Active site4401Proton acceptor By similarity
Binding site3381ATP By similarity

Amino acid modifications

Glycosylation601N-linked (GlcNAc...) Ref.4
Glycosylation1341N-linked (GlcNAc...) Ref.4
Glycosylation1651N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict3741A → P in AAA03544. Ref.1
Sequence conflict5451L → R in AAA03544. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P50488 [UniParc].

Last modified July 3, 2003. Version 2.
Checksum: 33C087871FD81AF0

FASTA74484,410
        10         20         30         40         50         60 
MNQKGTVRLK ALVLICLPLF LIATPVPVAV TEDDRQDRIE SEAAEKEWAN TLVSKVAQSN 

        70         80         90        100        110        120 
GTGTIKVSAP AKPTLRRMDN EEDEVISIEC VYYDEMECEK SGDCEITKKT CYSEAHLKAV 

       130        140        150        160        170        180 
GCLAVFGLPT QEINSTEPYL KVDKPQYKSL GCMPYQHADS MNCENESSCR QGRSFRGGIG 

       190        200        210        220        230        240 
MCCCSTNNCN MPDLIEMVNP SLKKDSDNSA LLWASTPSNM DLESLDKFPF YWIIIIALSV 

       250        260        270        280        290        300 
ILCIALLILA YVGWKFQQNK KEEIKKQQKI KFDMEKTDAL EAGNVPLVEP EEEMIEMVET 

       310        320        330        340        350        360 
PKELPITDFQ LISKGRFGKV FKAQYTPDSG EKRLVAVKKL NEFQKASFLA EKRIFDELNE 

       370        380        390        400        410        420 
YPKWYKSIVE FVCAEKIGDE YWIVTEFHER LSLYELLKNN VISITSANRI IMSMIDGLQF 

       430        440        450        460        470        480 
LHDDRPYFFG HPKKPIIHRD IKSKNILVKS DMTTCIADFG LARIYSYDIE QSDLLGQVGT 

       490        500        510        520        530        540 
KRYMSPEMLE GATEFTPTAF KAMDVYSMGL VMWEVISRTK LHQTDEPPNY QMPFQVIGFD 

       550        560        570        580        590        600 
PTIGLMRNYV VSKKERPQWR DEIIKHEYMS LLKKVTEEMW DPEACARITA GCAFARVWNH 

       610        620        630        640        650        660 
IMSSPDSSEG YHSGSSMKNR GVDDVEQSEK PEGIEEMQHY HASSPSKRQH PSPNPFFDSC 

       670        680        690        700        710        720 
PPPPPIPVIL ENGGILQPDN AEPEPEELPD LPIVEKIYDI ATNMLFSREE LDLMNAQRQV 

       730        740 
EYEAGADTRA STPTPSGTFG TFTT 

« Hide

References

« Hide 'large scale' references
[1]"The daf-4 gene encodes a bone morphogenetic protein receptor controlling C. elegans dauer larva development."
Estevez M., Attisano L., Wrana J.L., Albert P.S., Massague J., Riddle D.L.
Nature 365:644-649(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[3]"A Caenorhabditis elegans type I TGF beta receptor can function in the absence of type II kinase to promote larval development."
Gunther C.V., Georgi L.L., Riddle D.L.
Development 127:3337-3347(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[4]"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-60 AND ASN-134, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L23110 mRNA. Translation: AAA03544.1.
FO080356 Genomic DNA. Translation: CCD63118.1.
PIRD88462.
S38279.
RefSeqNP_498211.1. NM_065810.5.
UniGeneCel.6349.

3D structure databases

ProteinModelPortalP50488.
SMRP50488. Positions 302-597.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid41009. 15 interactions.
IntActP50488. 14 interactions.
MINTMINT-206636.

Proteomic databases

PaxDbP50488.
PRIDEP50488.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC05D2.1a; C05D2.1a; C05D2.1.
GeneID175781.
KEGGcel:CELE_C05D2.1.
UCSCC05D2.1a.1. c. elegans.

Organism-specific databases

CTD175781.
WormBaseC05D2.1a; CE16827; WBGene00000900; daf-4.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000018794.
InParanoidP50488.
KOK13597.
OMAKAMDVYS.
OrthoDBEOG7JHM5B.

Enzyme and pathway databases

BRENDA2.7.10.2. 1045.
SignaLinkP50488.

Gene expression databases

ArrayExpressP50488.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio889638.

Entry information

Entry nameDAF4_CAEEL
AccessionPrimary (citable) accession number: P50488
Secondary accession number(s): O45139
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 3, 2003
Last modified: April 16, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase