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Protein

Cell surface receptor daf-4

Gene

daf-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in TGF-beta pathway. May be a receptor for daf-7. Controls the dauer/nondauer developmental decision in a range of environmental conditions. Regulates body size and male tail patterning (PubMed:10887089). Involved in regulating entry into quiescence triggered by satiety (PubMed:18316030). Involved in sensitivity to CO2 levels (PubMed:18524955).3 Publications

Catalytic activityi

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei338ATPPROSITE-ProRule annotation1
Active sitei440Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi312 – 320ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • BMP binding Source: WormBase
  • receptor signaling protein serine/threonine kinase activity Source: InterPro
  • transmembrane receptor protein serine/threonine kinase activity Source: WormBase

GO - Biological processi

  • BMP signaling pathway Source: WormBase
  • dauer entry Source: UniProtKB
  • dauer larval development Source: WormBase
  • defense response to fungus Source: WormBase
  • innate immune response Source: WormBase
  • larval feeding behavior Source: UniProtKB
  • negative regulation of BMP signaling pathway Source: WormBase
  • negative regulation of dauer larval development Source: WormBase
  • nematode larval development Source: WormBase
  • oviposition Source: WormBase
  • positive regulation of BMP signaling pathway Source: WormBase
  • positive regulation of multicellular organism growth Source: WormBase
  • positive regulation of transcription from RNA polymerase II promoter Source: WormBase
  • regulation of cell morphogenesis Source: WormBase
  • regulation of dauer larval development Source: UniProtKB
  • regulation of eating behavior Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 1045.
SignaLinkiP50488.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell surface receptor daf-4 (EC:2.7.11.30)
Alternative name(s):
Abnormal dauer formation protein 4
Gene namesi
Name:daf-4
ORF Names:C05D2.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiC05D2.1a; CE16827; WBGene00000900; daf-4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini48 – 253ExtracellularSequence analysisAdd BLAST206
Transmembranei254 – 274HelicalSequence analysisAdd BLAST21
Topological domaini275 – 744CytoplasmicSequence analysisAdd BLAST470

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000002443132 – 744Cell surface receptor daf-4Add BLAST713

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi60N-linked (GlcNAc...)1 Publication1
Glycosylationi134N-linked (GlcNAc...)1 Publication1
Glycosylationi165N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiP50488.
PaxDbiP50488.
PeptideAtlasiP50488.
PRIDEiP50488.

Expressioni

Tissue specificityi

Pharynx, intestine, hypodermis and body wall muscles in L1 through to adult stages. Also expressed in head neurons, ventral cord and tail neurons. Subset of head neurons show coexpression with daf-1 when dauer/nondauer decision is made.1 Publication

Gene expression databases

BgeeiWBGene00000900.
ExpressionAtlasiP50488. baseline and differential.

Interactioni

Subunit structurei

May interact with daf-1 to regulate dauer larva development (PubMed:10887089). Interacts with sma-10 (PubMed:20502686).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
atp-2P465612EBI-296172,EBI-316294
scrm-1O457992EBI-296172,EBI-312683

GO - Molecular functioni

  • BMP binding Source: WormBase

Protein-protein interaction databases

BioGridi41009. 16 interactors.
IntActiP50488. 14 interactors.
MINTiMINT-206636.
STRINGi6239.C05D2.1a.

Structurei

3D structure databases

ProteinModelPortaliP50488.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini306 – 603Protein kinasePROSITE-ProRule annotationAdd BLAST298

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3653. Eukaryota.
ENOG410XS2Z. LUCA.
GeneTreeiENSGT00860000133897.
HOGENOMiHOG000018794.
InParanoidiP50488.
KOiK13597.
OMAiKAMDVYS.
OrthoDBiEOG091G0B6N.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR000333. TGFB_receptor.
[Graphical view]
PANTHERiPTHR23255. PTHR23255. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQKGTVRLK ALVLICLPLF LIATPVPVAV TEDDRQDRIE SEAAEKEWAN
60 70 80 90 100
TLVSKVAQSN GTGTIKVSAP AKPTLRRMDN EEDEVISIEC VYYDEMECEK
110 120 130 140 150
SGDCEITKKT CYSEAHLKAV GCLAVFGLPT QEINSTEPYL KVDKPQYKSL
160 170 180 190 200
GCMPYQHADS MNCENESSCR QGRSFRGGIG MCCCSTNNCN MPDLIEMVNP
210 220 230 240 250
SLKKDSDNSA LLWASTPSNM DLESLDKFPF YWIIIIALSV ILCIALLILA
260 270 280 290 300
YVGWKFQQNK KEEIKKQQKI KFDMEKTDAL EAGNVPLVEP EEEMIEMVET
310 320 330 340 350
PKELPITDFQ LISKGRFGKV FKAQYTPDSG EKRLVAVKKL NEFQKASFLA
360 370 380 390 400
EKRIFDELNE YPKWYKSIVE FVCAEKIGDE YWIVTEFHER LSLYELLKNN
410 420 430 440 450
VISITSANRI IMSMIDGLQF LHDDRPYFFG HPKKPIIHRD IKSKNILVKS
460 470 480 490 500
DMTTCIADFG LARIYSYDIE QSDLLGQVGT KRYMSPEMLE GATEFTPTAF
510 520 530 540 550
KAMDVYSMGL VMWEVISRTK LHQTDEPPNY QMPFQVIGFD PTIGLMRNYV
560 570 580 590 600
VSKKERPQWR DEIIKHEYMS LLKKVTEEMW DPEACARITA GCAFARVWNH
610 620 630 640 650
IMSSPDSSEG YHSGSSMKNR GVDDVEQSEK PEGIEEMQHY HASSPSKRQH
660 670 680 690 700
PSPNPFFDSC PPPPPIPVIL ENGGILQPDN AEPEPEELPD LPIVEKIYDI
710 720 730 740
ATNMLFSREE LDLMNAQRQV EYEAGADTRA STPTPSGTFG TFTT
Length:744
Mass (Da):84,410
Last modified:July 3, 2003 - v2
Checksum:i33C087871FD81AF0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti374A → P in AAA03544 (PubMed:8413626).Curated1
Sequence conflicti545L → R in AAA03544 (PubMed:8413626).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23110 mRNA. Translation: AAA03544.1.
FO080356 Genomic DNA. Translation: CCD63118.1.
PIRiD88462.
S38279.
RefSeqiNP_498211.1. NM_065810.5.
UniGeneiCel.6349.

Genome annotation databases

EnsemblMetazoaiC05D2.1a; C05D2.1a; WBGene00000900.
GeneIDi175781.
KEGGicel:CELE_C05D2.1.
UCSCiC05D2.1a.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23110 mRNA. Translation: AAA03544.1.
FO080356 Genomic DNA. Translation: CCD63118.1.
PIRiD88462.
S38279.
RefSeqiNP_498211.1. NM_065810.5.
UniGeneiCel.6349.

3D structure databases

ProteinModelPortaliP50488.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41009. 16 interactors.
IntActiP50488. 14 interactors.
MINTiMINT-206636.
STRINGi6239.C05D2.1a.

Proteomic databases

EPDiP50488.
PaxDbiP50488.
PeptideAtlasiP50488.
PRIDEiP50488.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC05D2.1a; C05D2.1a; WBGene00000900.
GeneIDi175781.
KEGGicel:CELE_C05D2.1.
UCSCiC05D2.1a.1. c. elegans.

Organism-specific databases

CTDi175781.
WormBaseiC05D2.1a; CE16827; WBGene00000900; daf-4.

Phylogenomic databases

eggNOGiKOG3653. Eukaryota.
ENOG410XS2Z. LUCA.
GeneTreeiENSGT00860000133897.
HOGENOMiHOG000018794.
InParanoidiP50488.
KOiK13597.
OMAiKAMDVYS.
OrthoDBiEOG091G0B6N.

Enzyme and pathway databases

BRENDAi2.7.10.2. 1045.
SignaLinkiP50488.

Miscellaneous databases

PROiP50488.

Gene expression databases

BgeeiWBGene00000900.
ExpressionAtlasiP50488. baseline and differential.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR000333. TGFB_receptor.
[Graphical view]
PANTHERiPTHR23255. PTHR23255. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAF4_CAEEL
AccessioniPrimary (citable) accession number: P50488
Secondary accession number(s): O45139
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 3, 2003
Last modified: November 30, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.