P50488 (DAF4_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell surface receptor daf-4
EC=2.7.11.30

Alternative name(s):
Abnormal dauer formation protein 4

Gene names

Name:	daf-4
ORF Names:	C05D2.1

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	744 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in TGF-beta pathway. May be a receptor for daf-7. Controls the dauer/nondauer developmental decision in a range of environmental conditions. Regulates body size and male tail patterning. Ref.3
Catalytic activity	ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.
Subunit structure	May interact with daf-1 to regulate dauer larva development. Ref.3
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Pharynx, intestine, hypodermis and body wall muscles in L1 through to adult stages. Also expressed in head neurons, ventral cord and tail neurons. Subset of head neurons show coexpression with daf-1 when dauer/nondauer decision is made. Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	BMP signaling pathway Inferred by curator Ref.1. Source: WormBase dauer larval development Inferred from mutant phenotype PubMed 10625546Ref.3PubMed 12717735PubMed 15254038Ref.1. Source: WormBase defense response to fungus Inferred from mutant phenotype PubMed 19198592. Source: WormBase innate immune response Inferred from mutant phenotype PubMed 19198592. Source: WormBase negative regulation of BMP signaling pathway Inferred from mutant phenotype PubMed 15254038. Source: WormBase oviposition Inferred from mutant phenotype PubMed 10625546. Source: WormBase positive regulation of BMP signaling pathway Inferred from mutant phenotype PubMed 15254038. Source: WormBase positive regulation of multicellular organism growth Inferred from mutant phenotype PubMed 10625546PubMed 12717735. Source: WormBase positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 19198592PubMed 9834189. Source: WormBase
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 9334330. Source: WormBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW transmembrane receptor protein serine/threonine kinase activity Inferred from sequence or structural similarity Ref.1. Source: WormBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
atp-2	P46561	2	EBI-296172,EBI-316294
scrm-1	O45799	2	EBI-296172,EBI-312683

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 744	713	Cell surface receptor daf-4		PRO_0000024431
Regions
	Topological domain	48 – 253	206	Extracellular Potential
	Transmembrane	254 – 274	21	Helical; Potential
	Topological domain	275 – 744	470	Cytoplasmic Potential
	Domain	306 – 603	298	Protein kinase
	Nucleotide binding	312 – 320	9	ATP By similarity
Sites
	Active site	440	1	Proton acceptor By similarity
	Binding site	338	1	ATP By similarity
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	134	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	165	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	374	1	A → P in AAA03544. Ref.1
	Sequence conflict	545	1	L → R in AAA03544. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P50488 [UniParc]. Last modified July 3, 2003. Version 2. Checksum: 33C087871FD81AF0	FASTA	744	84,410
	10 20 30 40 50 60 MNQKGTVRLK ALVLICLPLF LIATPVPVAV TEDDRQDRIE SEAAEKEWAN TLVSKVAQSN 70 80 90 100 110 120 GTGTIKVSAP AKPTLRRMDN EEDEVISIEC VYYDEMECEK SGDCEITKKT CYSEAHLKAV 130 140 150 160 170 180 GCLAVFGLPT QEINSTEPYL KVDKPQYKSL GCMPYQHADS MNCENESSCR QGRSFRGGIG 190 200 210 220 230 240 MCCCSTNNCN MPDLIEMVNP SLKKDSDNSA LLWASTPSNM DLESLDKFPF YWIIIIALSV 250 260 270 280 290 300 ILCIALLILA YVGWKFQQNK KEEIKKQQKI KFDMEKTDAL EAGNVPLVEP EEEMIEMVET 310 320 330 340 350 360 PKELPITDFQ LISKGRFGKV FKAQYTPDSG EKRLVAVKKL NEFQKASFLA EKRIFDELNE 370 380 390 400 410 420 YPKWYKSIVE FVCAEKIGDE YWIVTEFHER LSLYELLKNN VISITSANRI IMSMIDGLQF 430 440 450 460 470 480 LHDDRPYFFG HPKKPIIHRD IKSKNILVKS DMTTCIADFG LARIYSYDIE QSDLLGQVGT 490 500 510 520 530 540 KRYMSPEMLE GATEFTPTAF KAMDVYSMGL VMWEVISRTK LHQTDEPPNY QMPFQVIGFD 550 560 570 580 590 600 PTIGLMRNYV VSKKERPQWR DEIIKHEYMS LLKKVTEEMW DPEACARITA GCAFARVWNH 610 620 630 640 650 660 IMSSPDSSEG YHSGSSMKNR GVDDVEQSEK PEGIEEMQHY HASSPSKRQH PSPNPFFDSC 670 680 690 700 710 720 PPPPPIPVIL ENGGILQPDN AEPEPEELPD LPIVEKIYDI ATNMLFSREE LDLMNAQRQV 730 740 EYEAGADTRA STPTPSGTFG TFTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The daf-4 gene encodes a bone morphogenetic protein receptor controlling C. elegans dauer larva development." Estevez M., Attisano L., Wrana J.L., Albert P.S., Massague J., Riddle D.L. Nature 365:644-649(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"A Caenorhabditis elegans type I TGF beta receptor can function in the absence of type II kinase to promote larval development." Gunther C.V., Georgi L.L., Riddle D.L. Development 127:3337-3347(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[4]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-60 AND ASN-134, MASS SPECTROMETRY. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L23110 mRNA. Translation: AAA03544.1. FO080356 Genomic DNA. Translation: CCD63118.1.
PIR	D88462. S38279.
RefSeq	NP_498211.1. NM_065810.5.
3D structure databases
ProteinModelPortal	P50488.
SMR	P50488. Positions 299-624.
ModBase	Search...
Protein-protein interaction databases
IntAct	P50488. 14 interactions.
Proteomic databases
PaxDb	P50488.
PRIDE	P50488.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C05D2.1a; C05D2.1a; C05D2.1.
GeneID	175781.
KEGG	cel:CELE_C05D2.1.
UCSC	C05D2.1a.1. c. elegans.
Organism-specific databases
CTD	175781.
WormBase	C05D2.1a; CE16827; WBGene00000900; daf-4.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00560000076906.
HOGENOM	HOG000018794.
InParanoid	P50488.
KO	K13597.
OMA	EMVETPK.
Enzyme and pathway databases
BRENDA	2.7.10.2. 1045.
Gene expression databases
ArrayExpress	P50488.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	889638.

Entry information

Entry name

DAF4_CAEEL

Accession

Primary (citable) accession number: P50488
Secondary accession number(s): O45139

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 3, 2003
Last modified:	May 1, 2013
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project