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P50488

- DAF4_CAEEL

UniProt

P50488 - DAF4_CAEEL

Protein

Cell surface receptor daf-4

Gene

daf-4

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (03 Jul 2003)
      Previous versions | rss
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    Functioni

    Involved in TGF-beta pathway. May be a receptor for daf-7. Controls the dauer/nondauer developmental decision in a range of environmental conditions. Regulates body size and male tail patterning.1 Publication

    Catalytic activityi

    ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei338 – 3381ATPPROSITE-ProRule annotation
    Active sitei440 – 4401Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi312 – 3209ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. BMP binding Source: WormBase
    3. protein binding Source: IntAct
    4. receptor signaling protein serine/threonine kinase activity Source: InterPro
    5. transmembrane receptor protein serine/threonine kinase activity Source: WormBase

    GO - Biological processi

    1. BMP signaling pathway Source: WormBase
    2. dauer larval development Source: WormBase
    3. defense response to fungus Source: WormBase
    4. innate immune response Source: WormBase
    5. negative regulation of BMP signaling pathway Source: WormBase
    6. nematode larval development Source: WormBase
    7. oviposition Source: WormBase
    8. positive regulation of BMP signaling pathway Source: WormBase
    9. positive regulation of multicellular organism growth Source: WormBase
    10. positive regulation of transcription from RNA polymerase II promoter Source: WormBase
    11. regulation of cell morphogenesis Source: WormBase

    Keywords - Molecular functioni

    Developmental protein, Kinase, Receptor, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.2. 1045.
    SignaLinkiP50488.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell surface receptor daf-4 (EC:2.7.11.30)
    Alternative name(s):
    Abnormal dauer formation protein 4
    Gene namesi
    Name:daf-4
    ORF Names:C05D2.1
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiC05D2.1a; CE16827; WBGene00000900; daf-4.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: WormBase

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Chaini32 – 744713Cell surface receptor daf-4PRO_0000024431Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi60 – 601N-linked (GlcNAc...)1 Publication
    Glycosylationi134 – 1341N-linked (GlcNAc...)1 Publication
    Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP50488.
    PRIDEiP50488.

    Expressioni

    Tissue specificityi

    Pharynx, intestine, hypodermis and body wall muscles in L1 through to adult stages. Also expressed in head neurons, ventral cord and tail neurons. Subset of head neurons show coexpression with daf-1 when dauer/nondauer decision is made.1 Publication

    Gene expression databases

    ArrayExpressiP50488.

    Interactioni

    Subunit structurei

    May interact with daf-1 to regulate dauer larva development.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    atp-2P465612EBI-296172,EBI-316294
    scrm-1O457992EBI-296172,EBI-312683

    Protein-protein interaction databases

    BioGridi41009. 15 interactions.
    IntActiP50488. 14 interactions.
    MINTiMINT-206636.

    Structurei

    3D structure databases

    ProteinModelPortaliP50488.
    SMRiP50488. Positions 296-624.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini48 – 253206ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini275 – 744470CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei254 – 27421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini306 – 603298Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00730000111964.
    HOGENOMiHOG000018794.
    InParanoidiP50488.
    KOiK13597.
    OMAiKAMDVYS.
    OrthoDBiEOG7JHM5B.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR000333. TGFB_receptor.
    [Graphical view]
    PANTHERiPTHR23255. PTHR23255. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50488-1 [UniParc]FASTAAdd to Basket

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    MNQKGTVRLK ALVLICLPLF LIATPVPVAV TEDDRQDRIE SEAAEKEWAN    50
    TLVSKVAQSN GTGTIKVSAP AKPTLRRMDN EEDEVISIEC VYYDEMECEK 100
    SGDCEITKKT CYSEAHLKAV GCLAVFGLPT QEINSTEPYL KVDKPQYKSL 150
    GCMPYQHADS MNCENESSCR QGRSFRGGIG MCCCSTNNCN MPDLIEMVNP 200
    SLKKDSDNSA LLWASTPSNM DLESLDKFPF YWIIIIALSV ILCIALLILA 250
    YVGWKFQQNK KEEIKKQQKI KFDMEKTDAL EAGNVPLVEP EEEMIEMVET 300
    PKELPITDFQ LISKGRFGKV FKAQYTPDSG EKRLVAVKKL NEFQKASFLA 350
    EKRIFDELNE YPKWYKSIVE FVCAEKIGDE YWIVTEFHER LSLYELLKNN 400
    VISITSANRI IMSMIDGLQF LHDDRPYFFG HPKKPIIHRD IKSKNILVKS 450
    DMTTCIADFG LARIYSYDIE QSDLLGQVGT KRYMSPEMLE GATEFTPTAF 500
    KAMDVYSMGL VMWEVISRTK LHQTDEPPNY QMPFQVIGFD PTIGLMRNYV 550
    VSKKERPQWR DEIIKHEYMS LLKKVTEEMW DPEACARITA GCAFARVWNH 600
    IMSSPDSSEG YHSGSSMKNR GVDDVEQSEK PEGIEEMQHY HASSPSKRQH 650
    PSPNPFFDSC PPPPPIPVIL ENGGILQPDN AEPEPEELPD LPIVEKIYDI 700
    ATNMLFSREE LDLMNAQRQV EYEAGADTRA STPTPSGTFG TFTT 744
    Length:744
    Mass (Da):84,410
    Last modified:July 3, 2003 - v2
    Checksum:i33C087871FD81AF0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti374 – 3741A → P in AAA03544. (PubMed:8413626)Curated
    Sequence conflicti545 – 5451L → R in AAA03544. (PubMed:8413626)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23110 mRNA. Translation: AAA03544.1.
    FO080356 Genomic DNA. Translation: CCD63118.1.
    PIRiD88462.
    S38279.
    RefSeqiNP_498211.1. NM_065810.5.
    UniGeneiCel.6349.

    Genome annotation databases

    EnsemblMetazoaiC05D2.1a; C05D2.1a; WBGene00000900.
    GeneIDi175781.
    KEGGicel:CELE_C05D2.1.
    UCSCiC05D2.1a.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23110 mRNA. Translation: AAA03544.1 .
    FO080356 Genomic DNA. Translation: CCD63118.1 .
    PIRi D88462.
    S38279.
    RefSeqi NP_498211.1. NM_065810.5.
    UniGenei Cel.6349.

    3D structure databases

    ProteinModelPortali P50488.
    SMRi P50488. Positions 296-624.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 41009. 15 interactions.
    IntActi P50488. 14 interactions.
    MINTi MINT-206636.

    Proteomic databases

    PaxDbi P50488.
    PRIDEi P50488.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C05D2.1a ; C05D2.1a ; WBGene00000900 .
    GeneIDi 175781.
    KEGGi cel:CELE_C05D2.1.
    UCSCi C05D2.1a.1. c. elegans.

    Organism-specific databases

    CTDi 175781.
    WormBasei C05D2.1a ; CE16827 ; WBGene00000900 ; daf-4.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00730000111964.
    HOGENOMi HOG000018794.
    InParanoidi P50488.
    KOi K13597.
    OMAi KAMDVYS.
    OrthoDBi EOG7JHM5B.

    Enzyme and pathway databases

    BRENDAi 2.7.10.2. 1045.
    SignaLinki P50488.

    Miscellaneous databases

    NextBioi 889638.

    Gene expression databases

    ArrayExpressi P50488.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR000333. TGFB_receptor.
    [Graphical view ]
    PANTHERi PTHR23255. PTHR23255. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The daf-4 gene encodes a bone morphogenetic protein receptor controlling C. elegans dauer larva development."
      Estevez M., Attisano L., Wrana J.L., Albert P.S., Massague J., Riddle D.L.
      Nature 365:644-649(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. "A Caenorhabditis elegans type I TGF beta receptor can function in the absence of type II kinase to promote larval development."
      Gunther C.V., Georgi L.L., Riddle D.L.
      Development 127:3337-3347(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    4. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
      Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
      Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-60 AND ASN-134, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiDAF4_CAEEL
    AccessioniPrimary (citable) accession number: P50488
    Secondary accession number(s): O45139
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 3, 2003
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3