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Reviewed, UniProtKB/Swiss-Prot P50481 (LHX3_MOUSE)

Last modified June 16, 2009. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    LIM/homeobox protein Lhx3
      Short name=LIM homeobox protein 3
Alternative name(s):
    Homeobox protein LIM-3
    Homeobox protein P-LIM
Gene names
Name: Lhx3
Synonyms: Lim-3, Lim3, Plim
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1.

Subcellular location

Nucleus Probable.

Tissue specificity

Mostly expressed in the pituitary anterior and intermediate lobes of the adult mouse. It is also expressed in the pineal gland and transiently in the primordia of motor neurons including the spinal cord, pons and medulla oblongata.

Developmental stage

Expressed throughout pituitary development. Detected on embryonic day 11 (E11) in the primordium of the hypophysis. Following a maximum between E12 and E14, lower levels persisted into adulthood.

Domain

The LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic intractions with Pit-1, but not for basal transcriptional activation events.

Sequence similarities

Contains 1 homeobox DNA-binding domain.

Contains 2 LIM zinc-binding domains.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform LIM3A (identifier: P50481-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform LIM3B (identifier: P50481-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: MLLEAELDCHRERPGAPGASALCTFSRTP → MEARGELDPSRESAGGDLLLALLARRADLRR

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 400400LIM/homeobox protein Lhx3
PRO_0000075782

Regions

Domain34 – 8451LIM zinc-binding 1
Domain93 – 14755LIM zinc-binding 2
DNA binding160 – 21960Homeobox

Natural variations

Alternative sequence1 – 2929MLLEA…FSRTP → MEARGELDPSRESAGGDLLL ALLARRADLRR in isoform LIM3B.
VSP_003108

Experimental info

Sequence conflict261S → N in AAA98998. Ref.3
Sequence conflict761G → R in AAA73902. Ref.2
Sequence conflict311 – 3122EL → DV in AAA73902. Ref.2
Sequence conflict351 – 37828PSGPP…STESS → LQGPQVDPGPTHEGCWLEMA RTCPQRAG Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Isoform LIM3A [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: AD7A9453CFACC730

FASTA40044,010
        10         20         30         40         50         60 
MLLEAELDCH RERPGAPGAS ALCTFSRTPE IPMCAGCDQH ILDRFILKAL DRHWHSKCLK 

        70         80         90        100        110        120 
CSDCHVPLAE RCFSRGESVY CKDDFFKRFG TKCAACQLGI PPTQVVRRAQ DFVYHLHCFA 

       130        140        150        160        170        180 
CVVCKRQLAT GDEFYLMEDS RLVCKADYET AKQREAEATA KRPRTTITAK QLETLKSAYN 

       190        200        210        220        230        240 
TSPKPARHVR EQLSSETGLD MRVVQVWFQN RRAKEKRLKK DAGRQRWGQY FRNMKRSRGS 

       250        260        270        280        290        300 
SKSDKDSIQE GQDSDAEVSF TDEPSMADMG PANGLYSSLG EPAPALGRPV GGLGSFTLDH 

       310        320        330        340        350        360 
GGLTGPEQYR ELRPGSPYGI PPSPAAPQSL PGPQPLLSSL VYPDTNLSLV PSGPPGGPPP 

       370        380        390        400 
MRVLAGNGPS SDLSTESSSG YPDFPASPAS WLDEVDHAQF

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Isoform LIM3B.

Checksum: 45942CCDADC72080
Show »

FASTA40244,289

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References

[1]"The mouse homeoprotein mLIM-3 is expressed early in cells derived from the neuroepithelium and persists in adult pituitary."
Seidah N.G., Barale J.-C., Marcinkiewicz M., Mattei M.-G., Day R., Chretien M.
DNA Cell Biol. 13:1163-1180(1994) [PubMed: 7811383] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (LIM3A).
Tissue: Pituitary.
[2]"P-Lim, a LIM homeodomain factor, is expressed during pituitary organ and cell commitment and synergizes with Pit-1."
Bach I., Rhodes S.J., Pearse R.V. II, Heinzel T., Gloss B., Scully K.M., Sawchenko P.E., Rosenfeld M.G.
Proc. Natl. Acad. Sci. U.S.A. 92:2720-2724(1995) [PubMed: 7708713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (LIM3A).
Tissue: Pituitary.
[3]"Expression pattern of the murine LIM class homeobox gene Lhx3 in subsets of neural and neuroendocrine tissues."
Zhadanov A.B., Bertuzzi S., Taira M., Dawid I.B., Westphal H.
Dev. Dyn. 202:354-364(1995) [PubMed: 7626792] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (LIM3A AND LIM3B).
Strain: DBA/N and NIH Swiss.
Tissue: Pituitary.
+Additional computationally mapped references.

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Cross-references

Sequence databases

L33776 Genomic DNA. Translation: AAA62369.1.
L38857 mRNA. Translation: AAA73902.1.
L38249 mRNA. Translation: AAA98998.1.
L38248 mRNA. Translation: AAB64178.1.
IPIIPI00118214.
IPI00263557.
PIRI59360.
UniGeneMm.386765

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2JTNNMR-A28-153[»]
ModBaseSearch...

PTM databases

PhosphoSiteP50481.

Genome annotation databases

EnsemblENSMUSG00000026934. Mus musculus. [Contig view]

Organism-specific databases

MGIMGI:102673. Lhx3.

Phylogenomic databases

HOGENOMP50481.
HOVERGENP50481.
OMAP50481. FTDEPSM.

Gene expression databases

ArrayExpressP50481.
BgeeP50481.
CleanExMM_LHX3.
GermOnlineENSMUSG00000026934. Mus musculus.

Family and domain databases

InterProIPR001356. Homeobox.
IPR017970. Homeobox_CS.
IPR012287. Homeodomain-rel.
IPR001781. Znf_LIM.
[Graphical view]
Gene3DG3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
G3DSA:2.10.110.10. Znf_LIM. 1 hit.
PfamPF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
[Graphical view]
ProDomPD000010. Homeobox. 1 hit.
PD000094. LIM. 2 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
[Graphical view]
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry nameLHX3_MOUSE
AccessionPrimary (citable) accession number: P50481
Secondary accession number(s): Q61800, Q61801
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents