LIM/homeobox protein Lhx3 - Mus musculus (Mouse)

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Molecule processing

Regions

Natural variations

Experimental info

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Preferred order of sections

Molecule processing

Regions

Natural variations

Experimental info

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

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P50481 (LHX3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Protein names

Recommended name:
LIM/homeobox protein Lhx3
Short name=LIM homeobox protein 3

Alternative name(s):
Homeobox protein LIM-3
Homeobox protein P-LIM

Gene names

Name:	Lhx3
Synonyms:	Lim-3, Lim3, Plim

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Function	Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1. Ref.4
Subcellular location	Nucleus Probable.
Tissue specificity	Mostly expressed in the pituitary anterior and intermediate lobes of the adult mouse. It is also expressed in the pineal gland and transiently in the primordia of motor neurons including the spinal cord, pons and medulla oblongata.
Developmental stage	Expressed throughout pituitary development. Detected on embryonic day 11 (E11) in the primordium of the hypophysis. Following a maximum between E12 and E14, lower levels persisted into adulthood.
Domain	The LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
Sequence similarities	Contains 1 homeobox DNA-binding domain. Contains 2 LIM zinc-binding domains.

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Homeobox LIM domain Repeat
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	medial motor column neuron differentiation Inferred from genetic interaction. Source: MGI motor axon guidance Inferred from genetic interaction. Source: MGI negative regulation of apoptotic process Inferred from mutant phenotype. Source: MGI pituitary gland development Inferred from mutant phenotype. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI spinal cord association neuron differentiation Inferred from direct assay. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW ventral spinal cord interneuron specification Inferred from direct assay. Source: MGI
Cellular component	transcription factor complex Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB protein binding transcription factor activity Inferred from physical interaction. Source: MGI sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 400

400

LIM/homeobox protein Lhx3

PRO_0000075782

Domain

34 – 84

LIM zinc-binding 1

Domain

93 – 147

LIM zinc-binding 2

DNA binding

160 – 219

Homeobox

Alternative sequence

1 – 29

MLLEA…FSRTP → MEARGELDPSRESAGGDLLL ALLARRADLRR in isoform LIM3B.

VSP_003108

Sequence conflict

S → N in AAA98998. Ref.3

Sequence conflict

G → R in AAA73902. Ref.2

Sequence conflict

311 – 312

EL → DV in AAA73902. Ref.2

Sequence conflict

351 – 378

PSGPP…STESS → LQGPQVDPGPTHEGCWLEMA RTCPQRAG Ref.2

400

Helix Strand Turn

Details...

Sequence		Length	Mass (Da)
Isoform LIM3A [UniParc]. Last modified October 1, 1996. Version 1. Checksum: AD7A9453CFACC730 Show »	FASTA	400	44,010
10 20 30 40 50 60 MLLEAELDCH RERPGAPGAS ALCTFSRTPE IPMCAGCDQH ILDRFILKAL DRHWHSKCLK 70 80 90 100 110 120 CSDCHVPLAE RCFSRGESVY CKDDFFKRFG TKCAACQLGI PPTQVVRRAQ DFVYHLHCFA 130 140 150 160 170 180 CVVCKRQLAT GDEFYLMEDS RLVCKADYET AKQREAEATA KRPRTTITAK QLETLKSAYN 190 200 210 220 230 240 TSPKPARHVR EQLSSETGLD MRVVQVWFQN RRAKEKRLKK DAGRQRWGQY FRNMKRSRGS 250 260 270 280 290 300 SKSDKDSIQE GQDSDAEVSF TDEPSMADMG PANGLYSSLG EPAPALGRPV GGLGSFTLDH 310 320 330 340 350 360 GGLTGPEQYR ELRPGSPYGI PPSPAAPQSL PGPQPLLSSL VYPDTNLSLV PSGPPGGPPP 370 380 390 400 MRVLAGNGPS SDLSTESSSG YPDFPASPAS WLDEVDHAQF « Hide
Isoform LIM3B [UniParc]. Checksum: 45942CCDADC72080 Show »	FASTA	402	44,289
10 20 30 40 50 60 MEARGELDPS RESAGGDLLL ALLARRADLR REIPMCAGCD QHILDRFILK ALDRHWHSKC 70 80 90 100 110 120 LKCSDCHVPL AERCFSRGES VYCKDDFFKR FGTKCAACQL GIPPTQVVRR AQDFVYHLHC 130 140 150 160 170 180 FACVVCKRQL ATGDEFYLME DSRLVCKADY ETAKQREAEA TAKRPRTTIT AKQLETLKSA 190 200 210 220 230 240 YNTSPKPARH VREQLSSETG LDMRVVQVWF QNRRAKEKRL KKDAGRQRWG QYFRNMKRSR 250 260 270 280 290 300 GSSKSDKDSI QEGQDSDAEV SFTDEPSMAD MGPANGLYSS LGEPAPALGR PVGGLGSFTL 310 320 330 340 350 360 DHGGLTGPEQ YRELRPGSPY GIPPSPAAPQ SLPGPQPLLS SLVYPDTNLS LVPSGPPGGP 370 380 390 400 PPMRVLAGNG PSSDLSTESS SGYPDFPASP ASWLDEVDHA QF « Hide

[1]	"The mouse homeoprotein mLIM-3 is expressed early in cells derived from the neuroepithelium and persists in adult pituitary." Seidah N.G., Barale J.-C., Marcinkiewicz M., Mattei M.-G., Day R., Chretien M. DNA Cell Biol. 13:1163-1180(1994) [PubMed: 7811383] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (LIM3A). Tissue: Pituitary.
[2]	"P-Lim, a LIM homeodomain factor, is expressed during pituitary organ and cell commitment and synergizes with Pit-1." Bach I., Rhodes S.J., Pearse R.V. II, Heinzel T., Gloss B., Scully K.M., Sawchenko P.E., Rosenfeld M.G. Proc. Natl. Acad. Sci. U.S.A. 92:2720-2724(1995) [PubMed: 7708713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (LIM3A). Tissue: Pituitary.
[3]	"Expression pattern of the murine LIM class homeobox gene Lhx3 in subsets of neural and neuroendocrine tissues." Zhadanov A.B., Bertuzzi S., Taira M., Dawid I.B., Westphal H. Dev. Dyn. 202:354-364(1995) [PubMed: 7626792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (LIM3A AND LIM3B). Strain: DBA/N and NIH Swiss. Tissue: Pituitary.
[4]	"MRG1 binds to the LIM domain of Lhx2 and may function as a coactivator to stimulate glycoprotein hormone alpha-subunit gene expression." Glenn D.J., Maurer R.A. J. Biol. Chem. 274:36159-36167(1999) [PubMed: 10593900] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

EMBL
GenBank
DDBJ

L33776 Genomic DNA. Translation: AAA62369.1.
L38857 mRNA. Translation: AAA73902.1.
L38249 mRNA. Translation: AAA98998.1.
L38248 mRNA. Translation: AAB64178.1.

IPI

IPI00118214.
IPI00263557.

PIR

I59360.

RefSeq

NP_001034742.1. NM_001039653.1.

UniGene

Mm.386765.

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JTN	NMR	-	A	28-153	[»]
2RGT	X-ray	2.05	A/B	28-153	[»]

ProteinModelPortal

P50481.

SMR

P50481. Positions 28-152, 155-221.

ModBase

Search...

MINT

MINT-7009840.

STRING

P50481.

PhosphoSite

P50481.

PRIDE

P50481.

StructuralBiologyKnowledgebase

Search...

Ensembl

ENSMUST00000028302; ENSMUSP00000028302; ENSMUSG00000026934.
ENSMUST00000054099; ENSMUSP00000056822; ENSMUSG00000026934.

GeneID

16871.

KEGG

mmu:16871.

CTD

8022.

MGI

MGI:102673. Lhx3.

HOVERGEN

HBG006263.

OMA

FTDEPSM.

OrthoDB

EOG4SBDZ0.

ArrayExpress

P50481.

Bgee

P50481.

CleanEx

MM_LHX3.

Genevestigator

P50481.

GermOnline

ENSMUSG00000026934. Mus musculus.

InterPro

IPR001356. Homeobox.
IPR017970. Homeobox_CS.
IPR009057. Homeodomain-like.
IPR012287. Homeodomain-rel.
IPR001781. Znf_LIM.
[Graphical view]

Gene3D

G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
G3DSA:2.10.110.10. Znf_LIM. 2 hits.

K09374.

Pfam

PF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
[Graphical view]

SMART

SM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
[Graphical view]

SUPFAM

SSF46689. Homeodomain_like. 1 hit.

PROSITE

PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]

ProtoNet

Search...

SOURCE

Search...

Entry name

LHX3_MOUSE

Accession

Primary (citable) accession number: P50481
Secondary accession number(s): Q61800, Q61801

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform LIM3A (identifier: P50481-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform LIM3B (identifier: P50481-2)

The sequence of this isoform differs from the canonical sequence as follows:
1-29: MLLEAELDCHRERPGAPGASALCTFSRTP → MEARGELDPSRESAGGDLLLALLARRADLRR