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Protein

Insulin gene enhancer protein ISL-2

Gene

Isl2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi192 – 25160HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin gene enhancer protein ISL-2
Short name:
Islet-2
Gene namesi
Name:Isl2
Synonyms:Isl-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621849. Isl2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Insulin gene enhancer protein ISL-2PRO_0000075754Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei154 – 1541PhosphoserineBy similarity
Modified residuei157 – 1571PhosphoserineBy similarity
Modified residuei280 – 2801PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP50480.
PRIDEiP50480.

Interactioni

Subunit structurei

Interacts with LHX4.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021074.

Structurei

Secondary structure

1
360
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi201 – 21313Combined sources
Helixi219 – 22911Combined sources
Helixi233 – 24614Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BW5NMR-A191-255[»]
ProteinModelPortaliP50480.
SMRiP50480. Positions 191-255.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50480.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 8662LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini87 – 14963LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni273 – 30230LIM-binding domain (LID)By similarityAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 2 LIM zinc-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox, LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
HOGENOMiHOG000236304.
HOVERGENiHBG004671.
InParanoidiP50480.
KOiK18492.
PhylomeDBiP50480.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
2.10.110.10. 2 hits.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50480-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDIIFHYPF LGAMGDHSKK KPGTAMCVGC GSQIHDQFIL RVSPDLEWHA
60 70 80 90 100
ACLKCAECSQ YLDETCTCFV RDGKTYCKRD YVRLFGIKCA QCQVGFSSSD
110 120 130 140 150
LVMRARDSVY HIECFRCSVC SRQLLPGDEF SLREHELLCR ADHGLLLERA
160 170 180 190 200
AAGSPRSPGP LPGTPPGLHL PDAGSGQQVS LRTHVHKQAE KTTRVRTVLN
210 220 230 240 250
EKQLHTLRTC YAANPRPDAL MKEQLVEMTG LSPRVIRVWF QNKRCKDKKK
260 270 280 290 300
SILMKQLQQQ QHSDKASLQG LTGTLLVAGS PSAHENAVQG SAVEVQTYQP
310 320 330 340 350
PWKALSEFAL QSDLDQPAFQ QLVSFSESGS LGNSSGSDVT SLSSQLPDTP
360
NSMVPSPVET
Length:360
Mass (Da):39,675
Last modified:October 1, 1996 - v1
Checksum:iBE45E5AE7B6AA686
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35571 mRNA. Translation: AAA62161.1.
PIRiA55198.
RefSeqiNP_065204.1. NM_020471.1.
UniGeneiRn.10026.

Genome annotation databases

GeneIDi57233.
KEGGirno:57233.
UCSCiRGD:621849. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35571 mRNA. Translation: AAA62161.1.
PIRiA55198.
RefSeqiNP_065204.1. NM_020471.1.
UniGeneiRn.10026.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BW5NMR-A191-255[»]
ProteinModelPortaliP50480.
SMRiP50480. Positions 191-255.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021074.

Proteomic databases

PaxDbiP50480.
PRIDEiP50480.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi57233.
KEGGirno:57233.
UCSCiRGD:621849. rat.

Organism-specific databases

CTDi64843.
RGDi621849. Isl2.

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
HOGENOMiHOG000236304.
HOVERGENiHBG004671.
InParanoidiP50480.
KOiK18492.
PhylomeDBiP50480.

Miscellaneous databases

EvolutionaryTraceiP50480.
PROiP50480.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
2.10.110.10. 2 hits.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes."
    Tsuchida T., Ensini M., Morton S.B., Baldassare M., Edlund T., Jessell T.M., Pfaff S.L.
    Cell 79:957-970(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spinal cord.
  2. "The solution structure of the homeodomain of the rat insulin-gene enhancer protein Isl-1. Comparison with other homeodomains."
    Ippel H., Larsson G., Behravan G., Zdunek J., Lundqvist M., Schleucher J., Lycksell P.-O., Wijmenga S.
    J. Mol. Biol. 288:689-703(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 180-244.

Entry informationi

Entry nameiISL2_RAT
AccessioniPrimary (citable) accession number: P50480
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.