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Protein

Aerotaxis receptor

Gene

aer

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Signal transducer for aerotaxis. The aerotactic response is the accumulation of cells around air bubbles. The nature of the sensory stimulus detected by this protein is the proton motive force or cellular redox state. It uses a FAD prosthetic group as a redox sensor to monitor oxygen levels.2 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • signal transducer activity Source: EcoCyc

GO - Biological processi

  • positive aerotaxis Source: EcoCyc
  • signal transduction Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Chemotaxis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:G7595-MONOMER.
ECOL316407:JW3043-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aerotaxis receptor
Gene namesi
Name:aer
Synonyms:air, yqjJ
Ordered Locus Names:b3072, JW3043
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12955. aer.

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

  • Note: Predominantly localized to one cell pole in mid-to-late exponential phase, with a few smaller foci elsewhere in the cell.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 166166CytoplasmicSequence analysisAdd
BLAST
Transmembranei167 – 18620HelicalSequence analysisAdd
BLAST
Topological domaini187 – 1904PeriplasmicSequence analysis
Transmembranei191 – 20919HelicalSequence analysisAdd
BLAST
Topological domaini210 – 506297CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506Aerotaxis receptorPRO_0000110565Add
BLAST

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiP50466.
PRIDEiP50466.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-1130981,EBI-1130981

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4262399. 186 interactions.
DIPiDIP-9061N.
IntActiP50466. 4 interactions.
STRINGi511145.b3072.

Structurei

3D structure databases

ProteinModelPortaliP50466.
SMRiP50466. Positions 20-135, 208-505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini263 – 492230Methyl-accepting transducerPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 methyl-accepting transducer domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107RHT. Bacteria.
COG0840. LUCA.
COG2202. LUCA.
HOGENOMiHOG000148074.
InParanoidiP50466.
KOiK03776.
OMAiMPVRWRI.
OrthoDBiEOG6G4VQG.
PhylomeDBiP50466.

Family and domain databases

InterProiIPR004090. Chemotax_Me-accpt_rcpt.
IPR003660. HAMP_dom.
IPR004089. MCPsignal_dom.
IPR001610. PAC.
IPR000014. PAS.
IPR013655. PAS_fold_3.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF00015. MCPsignal. 1 hit.
PF08447. PAS_3. 1 hit.
[Graphical view]
PRINTSiPR00260. CHEMTRNSDUCR.
SMARTiSM00283. MA. 1 hit.
SM00086. PAC. 1 hit.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSHPYVTQQ NTPLADDTTL MSTTDLQSYI THANDTFVQV SGYTLQELQG
60 70 80 90 100
QPHNMVRHPD MPKAAFADMW FTLKKGEPWS GIVKNRRKNG DHYWVRANAV
110 120 130 140 150
PMVREGKISG YMSIRTRATD EEIAAVEPLY KALNAGRTSK RIHKGLVVRK
160 170 180 190 200
GWLGKLPSLP LRWRARGVMT LMFILLAAML WFVAAPVVTY ILCALVVLLA
210 220 230 240 250
SACFEWQIVR PIENVAHQAL KVATGERNSV EHLNRSDELG LTLRAVGQLG
260 270 280 290 300
LMCRWLINDV SSQVSSVRNG SETLAKGTDE LNEHTQQTVD NVQQTVATMN
310 320 330 340 350
QMAASVKQNS ATASAADKLS ITASNAAVQG GEAMTTVIKT MDDIADSTQR
360 370 380 390 400
IGTITSLIND IAFQTNILAL NAAVEAARAG EQGKGFAVVA GEVRHLASRS
410 420 430 440 450
ANAANDIRKL IDASADKVQS GSQQVHAAGR TMEDIVAQVK NVTQLIAQIS
460 470 480 490 500
HSTLEQADGL SSLTRAVDEL NLITQKNAEL VEESAQVSAM VKHRASRLED

AVTVLH
Length:506
Mass (Da):55,066
Last modified:October 1, 1996 - v1
Checksum:i913DEBCF14E3FD08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28379 Genomic DNA. Translation: AAA89151.1.
U00096 Genomic DNA. Translation: AAC76107.1.
AP009048 Genomic DNA. Translation: BAE77122.1.
PIRiE65095.
RefSeqiNP_417543.1. NC_000913.3.
WP_000094721.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76107; AAC76107; b3072.
BAE77122; BAE77122; BAE77122.
GeneIDi945301.
KEGGiecj:JW3043.
eco:b3072.
PATRICi32121560. VBIEscCol129921_3166.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28379 Genomic DNA. Translation: AAA89151.1.
U00096 Genomic DNA. Translation: AAC76107.1.
AP009048 Genomic DNA. Translation: BAE77122.1.
PIRiE65095.
RefSeqiNP_417543.1. NC_000913.3.
WP_000094721.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP50466.
SMRiP50466. Positions 20-135, 208-505.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262399. 186 interactions.
DIPiDIP-9061N.
IntActiP50466. 4 interactions.
STRINGi511145.b3072.

Proteomic databases

PaxDbiP50466.
PRIDEiP50466.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76107; AAC76107; b3072.
BAE77122; BAE77122; BAE77122.
GeneIDi945301.
KEGGiecj:JW3043.
eco:b3072.
PATRICi32121560. VBIEscCol129921_3166.

Organism-specific databases

EchoBASEiEB2789.
EcoGeneiEG12955. aer.

Phylogenomic databases

eggNOGiENOG4107RHT. Bacteria.
COG0840. LUCA.
COG2202. LUCA.
HOGENOMiHOG000148074.
InParanoidiP50466.
KOiK03776.
OMAiMPVRWRI.
OrthoDBiEOG6G4VQG.
PhylomeDBiP50466.

Enzyme and pathway databases

BioCyciEcoCyc:G7595-MONOMER.
ECOL316407:JW3043-MONOMER.

Miscellaneous databases

PROiP50466.

Family and domain databases

InterProiIPR004090. Chemotax_Me-accpt_rcpt.
IPR003660. HAMP_dom.
IPR004089. MCPsignal_dom.
IPR001610. PAC.
IPR000014. PAS.
IPR013655. PAS_fold_3.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF00015. MCPsignal. 1 hit.
PF08447. PAS_3. 1 hit.
[Graphical view]
PRINTSiPR00260. CHEMTRNSDUCR.
SMARTiSM00283. MA. 1 hit.
SM00086. PAC. 1 hit.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "A signal transducer for aerotaxis in Escherichia coli."
    Bibikov S.I., Biran R., Rudd K.E., Parkinson J.S.
    J. Bacteriol. 179:4075-4079(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "The Aer protein and the serine chemoreceptor Tsr independently sense intracellular energy levels and transduce oxygen, redox, and energy signals for Escherichia coli behavior."
    Rebbapragada A., Johnson M.S., Harding G.P., Zuccarelli A.J., Fletcher H.M., Zhulin I.B., Taylor B.L.
    Proc. Natl. Acad. Sci. U.S.A. 94:10541-10546(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Isolation and identification of new inner membrane-associated proteins that localize to cell poles in Escherichia coli."
    Li G., Young K.D.
    Mol. Microbiol. 84:276-295(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiAER_ECOLI
AccessioniPrimary (citable) accession number: P50466
Secondary accession number(s): Q2M9D4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.