Reviewed, UniProtKB/Swiss-Prot P50466 (AER_ECOLI)

Names and origin

Protein names

Recommended name:
    Aerotaxis receptor

Gene names

Name:	aer
Synonyms:	air, yqjJ
Ordered Locus Names:	b3072, JW3043

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Signal transducer for aerotaxis. The aerotactic response is the accumulation of cells around air bubbles. The nature of the sensory stimulus detected by this protein is the proton motive force or cellular redox state. It uses a FAD prosthetic group as a redox sensor to monitor oxygen levels. Ref.3 Ref.4
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Contains 1 methyl-accepting transducer domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Transducer
PTM	Methylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Aerotaxis receptor		PRO_0000110565
Regions
	Topological domain	1 – 166	166	Cytoplasmic Potential
	Transmembrane	167 – 186	20	Potential
	Topological domain	187 – 190	4	Periplasmic Potential
	Transmembrane	191 – 209	19	Potential
	Topological domain	210 – 506	297	Cytoplasmic Potential
	Domain	263 – 492	230	Methyl-accepting transducer

Sequences

	Sequence		Length	Mass (Da)
	P50466-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 913DEBCF14E3FD08	FASTA	506	55,066
	10 20 30 40 50 60 MSSHPYVTQQ NTPLADDTTL MSTTDLQSYI THANDTFVQV SGYTLQELQG QPHNMVRHPD 70 80 90 100 110 120 MPKAAFADMW FTLKKGEPWS GIVKNRRKNG DHYWVRANAV PMVREGKISG YMSIRTRATD 130 140 150 160 170 180 EEIAAVEPLY KALNAGRTSK RIHKGLVVRK GWLGKLPSLP LRWRARGVMT LMFILLAAML 190 200 210 220 230 240 WFVAAPVVTY ILCALVVLLA SACFEWQIVR PIENVAHQAL KVATGERNSV EHLNRSDELG 250 260 270 280 290 300 LTLRAVGQLG LMCRWLINDV SSQVSSVRNG SETLAKGTDE LNEHTQQTVD NVQQTVATMN 310 320 330 340 350 360 QMAASVKQNS ATASAADKLS ITASNAAVQG GEAMTTVIKT MDDIADSTQR IGTITSLIND 370 380 390 400 410 420 IAFQTNILAL NAAVEAARAG EQGKGFAVVA GEVRHLASRS ANAANDIRKL IDASADKVQS 430 440 450 460 470 480 GSQQVHAAGR TMEDIVAQVK NVTQLIAQIS HSTLEQADGL SSLTRAVDEL NLITQKNAEL 490 500 VEESAQVSAM VKHRASRLED AVTVLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"A signal transducer for aerotaxis in Escherichia coli." Bibikov S.I., Biran R., Rudd K.E., Parkinson J.S. J. Bacteriol. 179:4075-4079(1997) [PubMed: 9190831] [Abstract] Cited for: FUNCTION.
[4]	"The Aer protein and the serine chemoreceptor Tsr independently sense intracellular energy levels and transduce oxygen, redox, and energy signals for Escherichia coli behavior." Rebbapragada A., Johnson M.S., Harding G.P., Zuccarelli A.J., Fletcher H.M., Zhulin I.B., Taylor B.L. Proc. Natl. Acad. Sci. U.S.A. 94:10541-10546(1997) [PubMed: 9380671] [Abstract] Cited for: FUNCTION.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U28379 Genomic DNA. Translation: AAA89151.1. U00096 Genomic DNA. Translation: AAC76107.1. AP009048 Genomic DNA. Translation: BAE77122.1.
PIR	E65095.
RefSeq	AP_003621.1. NP_417543.1.
3D structure databases
HSSP	HSSP built from PDB template 1QU7 based on UniProtKB P02942.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9061N.
Genome annotation databases
GeneID	945301.
GenomeReviews	Gene locus JW3043 in contig AP009048_GR. Gene locus b3072 in contig U00096_GR.
KEGG	ecj:JW3043. eco:b3072.
Organism-specific databases
EchoBASE	EB2789.
EcoGene	EG12955. aer.
CMR	Search...
Phylogenomic databases
HOGENOM	P50466.
OMA	P50466. LAVMLWF.
Enzyme and pathway databases
BioCyc	EcoCyc:G7595-MON.
Family and domain databases
InterPro	IPR004090. Chemotax_Me-accpt_rcpt. IPR004089. Chemotax_Me-accpt_rcpt_sig. IPR003660. HAMP_linker_domain. IPR001610. PAC. IPR000014. PAS. IPR013655. PAS_fold_3. [Graphical view]
Pfam	PF00672. HAMP. 1 hit. PF00015. MCPsignal. 1 hit. PF08447. PAS_3. 1 hit. [Graphical view]
PRINTS	PR00260. CHEMTRNSDUCR.
SMART	SM00283. MA. 1 hit. SM00086. PAC. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00229. sensory_box. 1 hit.
PROSITE	PS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AER_ECOLI

Accession

Primary (citable) accession number: P50466
Secondary accession number(s): Q2M9D4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)