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Protein

Cysteine and glycine-rich protein 3

Gene

Csrp3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Positive regulator of myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation (By similarity).By similarity

GO - Molecular functioni

  • MRF binding Source: MGI
  • RNA polymerase II sequence-specific DNA binding transcription factor binding Source: MGI
  • zinc ion binding Source: InterPro

GO - Biological processi

  • blood vessel remodeling Source: RGD
  • cell differentiation Source: UniProtKB-KW
  • muscle organ development Source: UniProtKB-KW
  • positive regulation of myotube differentiation Source: MGI
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Myogenesis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine and glycine-rich protein 3
Alternative name(s):
Cysteine-rich protein 3
Short name:
CRP3
LIM domain protein, cardiac
Muscle LIM protein
Gene namesi
Name:Csrp3
Synonyms:Clp, Mlp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi71092. Csrp3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytoskeleton Source: UniProtKB-SubCell
  • nucleus Source: RGD
  • Z disc Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 194194Cysteine and glycine-rich protein 3PRO_0000075729Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei95 – 951PhosphoserineBy similarity
Modified residuei111 – 1111PhosphoserineBy similarity
Modified residuei153 – 1531PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP50463.
PRIDEiP50463.

PTM databases

iPTMnetiP50463.
PhosphoSiteiP50463.

Expressioni

Tissue specificityi

High in striated muscle and adult heart.

Interactioni

Subunit structurei

Interacts with LDHD. Interacts with GLRX3 (via C-terminus).2 Publications

GO - Molecular functioni

  • MRF binding Source: MGI
  • RNA polymerase II sequence-specific DNA binding transcription factor binding Source: MGI

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019310.

Structurei

3D structure databases

ProteinModelPortaliP50463.
SMRiP50463. Positions 1-178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 6152LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini120 – 17152LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 696Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi63 – 7816Gly-richAdd
BLAST
Compositional biasi177 – 1859Gly-rich

Sequence similaritiesi

Contains 2 LIM zinc-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiENOG410ITI8. Eukaryota.
ENOG410Z840. LUCA.
HOGENOMiHOG000111233.
HOVERGENiHBG051143.
InParanoidiP50463.
KOiK09377.
PhylomeDBiP50463.

Family and domain databases

Gene3Di2.10.110.10. 2 hits.
InterProiIPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 2 hits.
[Graphical view]
SMARTiSM00132. LIM. 2 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50463-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNWGGGAKC GACDKTVYHA EEIQCNGRSF HKTCFHCMAC RKALDSTTVA
60 70 80 90 100
AHESEIYCKV CYGRKYGPKG IGFGQGAGCL STDTGEHLGL QFQQSPKPAR
110 120 130 140 150
AATTSNPSKF SAKFGESEKC PRCGKSVYAA EKVMGGGKPW HKTCFPCAIC
160 170 180 190
GKSLESTNVT DKDGELYCKV CYAKNFGPTG IGFGGLTHQV EKKE
Length:194
Mass (Da):20,803
Last modified:October 1, 1996 - v1
Checksum:i2D6848C271BA7EAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81193 mRNA. Translation: CAA57065.1.
PIRiA55099.
RefSeqiNP_476485.1. NM_057144.1.
UniGeneiRn.11345.

Genome annotation databases

GeneIDi117505.
KEGGirno:117505.
UCSCiRGD:71092. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81193 mRNA. Translation: CAA57065.1.
PIRiA55099.
RefSeqiNP_476485.1. NM_057144.1.
UniGeneiRn.11345.

3D structure databases

ProteinModelPortaliP50463.
SMRiP50463. Positions 1-178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019310.

PTM databases

iPTMnetiP50463.
PhosphoSiteiP50463.

Proteomic databases

PaxDbiP50463.
PRIDEiP50463.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi117505.
KEGGirno:117505.
UCSCiRGD:71092. rat.

Organism-specific databases

CTDi8048.
RGDi71092. Csrp3.

Phylogenomic databases

eggNOGiENOG410ITI8. Eukaryota.
ENOG410Z840. LUCA.
HOGENOMiHOG000111233.
HOVERGENiHBG051143.
InParanoidiP50463.
KOiK09377.
PhylomeDBiP50463.

Miscellaneous databases

NextBioi620251.
PROiP50463.

Family and domain databases

Gene3Di2.10.110.10. 2 hits.
InterProiIPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 2 hits.
[Graphical view]
SMARTiSM00132. LIM. 2 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Muscle LIM protein, a novel essential regulator of myogenesis, promotes myogenic differentiation."
    Arber S., Halder G., Caroni P.
    Cell 79:221-231(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Lewis.
    Tissue: Skeletal muscle.
  2. "Identification of putative mammalian D-lactate dehydrogenase enzymes."
    Flick M.J., Konieczny S.F.
    Biochem. Biophys. Res. Commun. 295:910-916(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LDHD.
  3. "PICOT attenuates cardiac hypertrophy by disrupting calcineurin-NFAT signaling."
    Jeong D., Kim J.M., Cha H., Oh J.G., Park J., Yun S.H., Ju E.S., Jeon E.S., Hajjar R.J., Park W.J.
    Circ. Res. 102:711-719(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GLRX3.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-153, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCSRP3_RAT
AccessioniPrimary (citable) accession number: P50463
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 20, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.