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P50462

- CSRP3_MOUSE

UniProt

P50462 - CSRP3_MOUSE

Protein

Cysteine and glycine-rich protein 3

Gene

Csrp3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Positive regulator of myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation.2 Publications

    GO - Molecular functioni

    1. actinin binding Source: BHF-UCL
    2. protein binding Source: UniProtKB
    3. structural constituent of muscle Source: BHF-UCL
    4. telethonin binding Source: BHF-UCL
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cardiac muscle contraction Source: BHF-UCL
    2. cardiac muscle hypertrophy Source: Ensembl
    3. cardiac muscle tissue development Source: MGI
    4. cardiac myofibril assembly Source: BHF-UCL
    5. cellular calcium ion homeostasis Source: MGI
    6. detection of muscle stretch Source: BHF-UCL
    7. muscle organ development Source: UniProtKB-KW
    8. protein localization to organelle Source: BHF-UCL
    9. regulation of the force of heart contraction Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Myogenesis

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine and glycine-rich protein 3
    Alternative name(s):
    Cysteine-rich protein 3
    Short name:
    CRP3
    LIM domain protein, cardiac
    Muscle LIM protein
    Gene namesi
    Name:Csrp3
    Synonyms:Clp, Mlp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1330824. Csrp3.

    Subcellular locationi

    Nucleus Curated. Cytoplasm 1 Publication. Cytoplasmcytoskeleton Curated. CytoplasmmyofibrilsarcomereZ line 1 Publication
    Note: Mainly cytoplasmic. In the nucleus it associates with the actin cytoskeleton Potential. In the Z line, found associated with GLRX3 (via C-terminus).Curated

    GO - Cellular componenti

    1. cytoskeleton Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. Z disc Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mutant mice developed dilated cardiomyopathy with hypertrophy and heart failure after birth. Ultrastructural analysis revealed a dramatic disruption of cardiomyocyte cytoarchitecture. At birth, these hearts are not hypertrophic, but already abnormally soft, with cell-autonomous and Csrp3-sensitive alterations in cytoarchitecture. The morphological, functional, and molecular features of the cardiac phenotype in mutant adult mice are undistinguishable from those seen in human heart failure resulting from dilated cardiomyopathy of various etiolologies, these mice can thus be used as model. Heterozygous mice display a more pronounced left ventricular dilation and systolic dysfunction and decreased survival after myocardial infaction.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi4 – 41W → R: Mice develop an age- and gene dosage-dependent hypertrophic cardiomyopathy and heart failure phenotype, characterized by almost complete loss of contractile reserve under catecholamine induced stress. They display increased in septum wall thickness, fractional shortening, and wall thickness per diameter (h/r). There is also evidence for skeletal muscle pathology. In addition, homozygous mutants show increased left ventricle (LC) mass per body weight (BW) and significantly reduced body weight. An increased nuclear localization of Csrp3 is also observed. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 194194Cysteine and glycine-rich protein 3PRO_0000075728Add
    BLAST

    Proteomic databases

    MaxQBiP50462.
    PaxDbiP50462.
    PRIDEiP50462.

    PTM databases

    PhosphoSiteiP50462.

    Expressioni

    Gene expression databases

    ArrayExpressiP50462.
    BgeeiP50462.
    CleanExiMM_CSRP3.
    GenevestigatoriP50462.

    Interactioni

    Subunit structurei

    Interacts with LDHD By similarity. Interacts with GLRX3.By similarity1 Publication

    Protein-protein interaction databases

    IntActiP50462. 2 interactions.
    MINTiMINT-4092342.
    STRINGi10090.ENSMUSP00000032658.

    Structurei

    3D structure databases

    ProteinModelPortaliP50462.
    SMRiP50462. Positions 1-178.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini10 – 6152LIM zinc-binding 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini120 – 17152LIM zinc-binding 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi64 – 696Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi63 – 7816Gly-richAdd
    BLAST
    Compositional biasi177 – 1859Gly-rich

    Sequence similaritiesi

    Contains 2 LIM zinc-binding domains.PROSITE-ProRule annotation

    Keywords - Domaini

    LIM domain, Repeat

    Phylogenomic databases

    eggNOGiNOG294681.
    HOGENOMiHOG000111233.
    HOVERGENiHBG051143.
    InParanoidiP50462.
    KOiK09377.
    OMAiQSPKQAR.
    OrthoDBiEOG7CK39M.
    PhylomeDBiP50462.
    TreeFamiTF313758.

    Family and domain databases

    Gene3Di2.10.110.10. 2 hits.
    InterProiIPR001781. Znf_LIM.
    [Graphical view]
    PfamiPF00412. LIM. 2 hits.
    [Graphical view]
    SMARTiSM00132. LIM. 2 hits.
    [Graphical view]
    PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
    PS50023. LIM_DOMAIN_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50462-1 [UniParc]FASTAAdd to Basket

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    MPNWGGGAKC GACEKTVYHA EEIQCNGRSF HKTCFHCMAC RKALDSTTVA    50
    AHESEIYCKV CYGRRYGPKG IGFGQGAGCL STDTGEHLGL QFQQSPKPAR 100
    AATTSNPSKF SAKFGESEKC PRCGKSVYAA EKVMGGGKPW HKTCFRCAIC 150
    GKSLESTNVT DKDGELYCKV CYAKNFGPTG IGFGGLTQQV EKKE 194
    Length:194
    Mass (Da):20,895
    Last modified:October 1, 1996 - v1
    Checksum:iE35CF30CA17CB227
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49883 mRNA. Translation: CAA90039.1.
    D88791 mRNA. Translation: BAA13721.1.
    BC061131 mRNA. Translation: AAH61131.1.
    CCDSiCCDS21305.1.
    PIRiS57472.
    RefSeqiNP_001185770.1. NM_001198841.1.
    NP_038836.1. NM_013808.4.
    XP_006540658.1. XM_006540595.1.
    UniGeneiMm.17235.
    Mm.488132.

    Genome annotation databases

    EnsembliENSMUST00000032658; ENSMUSP00000032658; ENSMUSG00000030470.
    ENSMUST00000167786; ENSMUSP00000129378; ENSMUSG00000030470.
    GeneIDi13009.
    KEGGimmu:13009.
    UCSCiuc009hax.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49883 mRNA. Translation: CAA90039.1 .
    D88791 mRNA. Translation: BAA13721.1 .
    BC061131 mRNA. Translation: AAH61131.1 .
    CCDSi CCDS21305.1.
    PIRi S57472.
    RefSeqi NP_001185770.1. NM_001198841.1.
    NP_038836.1. NM_013808.4.
    XP_006540658.1. XM_006540595.1.
    UniGenei Mm.17235.
    Mm.488132.

    3D structure databases

    ProteinModelPortali P50462.
    SMRi P50462. Positions 1-178.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P50462. 2 interactions.
    MINTi MINT-4092342.
    STRINGi 10090.ENSMUSP00000032658.

    PTM databases

    PhosphoSitei P50462.

    Proteomic databases

    MaxQBi P50462.
    PaxDbi P50462.
    PRIDEi P50462.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032658 ; ENSMUSP00000032658 ; ENSMUSG00000030470 .
    ENSMUST00000167786 ; ENSMUSP00000129378 ; ENSMUSG00000030470 .
    GeneIDi 13009.
    KEGGi mmu:13009.
    UCSCi uc009hax.2. mouse.

    Organism-specific databases

    CTDi 8048.
    MGIi MGI:1330824. Csrp3.

    Phylogenomic databases

    eggNOGi NOG294681.
    HOGENOMi HOG000111233.
    HOVERGENi HBG051143.
    InParanoidi P50462.
    KOi K09377.
    OMAi QSPKQAR.
    OrthoDBi EOG7CK39M.
    PhylomeDBi P50462.
    TreeFami TF313758.

    Miscellaneous databases

    NextBioi 282844.
    PROi P50462.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P50462.
    Bgeei P50462.
    CleanExi MM_CSRP3.
    Genevestigatori P50462.

    Family and domain databases

    Gene3Di 2.10.110.10. 2 hits.
    InterProi IPR001781. Znf_LIM.
    [Graphical view ]
    Pfami PF00412. LIM. 2 hits.
    [Graphical view ]
    SMARTi SM00132. LIM. 2 hits.
    [Graphical view ]
    PROSITEi PS00478. LIM_DOMAIN_1. 2 hits.
    PS50023. LIM_DOMAIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Murine MLP: cloning and expression in the embryonic head."
      Harrod G.V., Kettunen P.J., Jowett A.K.
      J. Craniofac. Genet. Dev. Biol. 16:65-73(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: MF1.
      Tissue: Mandible.
    2. Hashimoto N., Ogashiwa M.
      Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    4. "MLP-deficient mice exhibit a disruption of cardiac cytoarchitectural organization, dilated cardiomyopathy, and heart failure."
      Arber S., Hunter J.J., Ross J. Jr., Hongo M., Sansig G., Borg J., Perriard J.C., Chien K.R., Caroni P.
      Cell 88:393-403(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
      Strain: 129/Sv.
    5. "Attenuation of cardiac remodeling after myocardial infarction by muscle LIM protein-calcineurin signaling at the sarcomeric Z-disc."
      Heineke J., Ruetten H., Willenbockel C., Gross S.C., Naguib M., Schaefer A., Kempf T., Hilfiker-Kleiner D., Caroni P., Kraft T., Kaiser R.A., Molkentin J.D., Drexler H., Wollert K.C.
      Proc. Natl. Acad. Sci. U.S.A. 102:1655-1660(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    6. "PICOT attenuates cardiac hypertrophy by disrupting calcineurin-NFAT signaling."
      Jeong D., Kim J.M., Cha H., Oh J.G., Park J., Yun S.H., Ju E.S., Jeon E.S., Hajjar R.J., Park W.J.
      Circ. Res. 102:711-719(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GLRX3, SUBCELLULAR LOCATION.
    7. Cited for: MUTAGENESIS OF TRP-4.

    Entry informationi

    Entry nameiCSRP3_MOUSE
    AccessioniPrimary (citable) accession number: P50462
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3