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P50462 (CSRP3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cysteine and glycine-rich protein 3
Alternative name(s):
Cysteine-rich protein 3
Short name=CRP3
LIM domain protein, cardiac
Muscle LIM protein
Gene names
Name:Csrp3
Synonyms:Clp, Mlp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length194 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Positive regulator of myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation. Ref.4 Ref.5

Subunit structure

Interacts with LDHD By similarity. Interacts with GLRX3. Ref.6

Subcellular location

Nucleus Potential. Cytoplasm. Cytoplasmcytoskeleton Potential. CytoplasmmyofibrilsarcomereZ line. Note: Mainly cytoplasmic. In the nucleus it associates with the actin cytoskeleton Potential. In the Z line, found associated with GLRX3 (via C-terminus). Ref.6

Disruption phenotype

Mutant mice developed dilated cardiomyopathy with hypertrophy and heart failure after birth. Ultrastructural analysis revealed a dramatic disruption of cardiomyocyte cytoarchitecture. At birth, these hearts are not hypertrophic, but already abnormally soft, with cell-autonomous and Csrp3-sensitive alterations in cytoarchitecture. The morphological, functional, and molecular features of the cardiac phenotype in mutant adult mice are undistinguishable from those seen in human heart failure resulting from dilated cardiomyopathy of various etiolologies, these mice can thus be used as model. Heterozygous mice display a more pronounced left ventricular dilation and systolic dysfunction and decreased survival after myocardial infaction. Ref.4 Ref.5

Sequence similarities

Contains 2 LIM zinc-binding domains.

Ontologies

Keywords
   Biological processDifferentiation
Myogenesis
   Cellular componentCytoplasm
Cytoskeleton
Nucleus
   DomainLIM domain
Repeat
   LigandMetal-binding
Zinc
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcardiac muscle contraction

Inferred from mutant phenotype PubMed 12507422. Source: BHF-UCL

cardiac muscle hypertrophy

Inferred from electronic annotation. Source: Ensembl

cardiac muscle tissue development

Inferred from genetic interaction PubMed 10555147. Source: MGI

cardiac myofibril assembly

Inferred from mutant phenotype PubMed 12507422. Source: BHF-UCL

cellular calcium ion homeostasis

Inferred from genetic interaction PubMed 10555147. Source: MGI

detection of muscle stretch

Inferred from mutant phenotype PubMed 12507422. Source: BHF-UCL

muscle organ development

Inferred from electronic annotation. Source: UniProtKB-KW

protein localization to organelle

Inferred from mutant phenotype PubMed 12507422. Source: BHF-UCL

regulation of the force of heart contraction

Inferred from genetic interaction PubMed 10555147. Source: MGI

   Cellular_componentZ disc

Inferred from direct assay Ref.5. Source: MGI

cytoskeleton

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionactinin binding

Inferred from direct assay PubMed 12507422. Source: BHF-UCL

structural constituent of muscle

Inferred from mutant phenotype PubMed 12507422. Source: BHF-UCL

telethonin binding

Inferred from physical interaction PubMed 12507422. Source: BHF-UCL

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 194194Cysteine and glycine-rich protein 3
PRO_0000075728

Regions

Domain10 – 6152LIM zinc-binding 1
Domain120 – 17152LIM zinc-binding 2
Motif64 – 696Nuclear localization signal Potential
Compositional bias63 – 7816Gly-rich
Compositional bias177 – 1859Gly-rich

Experimental info

Mutagenesis41W → R: Mice develop an age- and gene dosage-dependent hypertrophic cardiomyopathy and heart failure phenotype, characterized by almost complete loss of contractile reserve under catecholamine induced stress. They display increased in septum wall thickness, fractional shortening, and wall thickness per diameter (h/r). There is also evidence for skeletal muscle pathology. In addition, homozygous mutants show increased left ventricle (LC) mass per body weight (BW) and significantly reduced body weight. An increased nuclear localization of Csrp3 is also observed. Ref.7

Sequences

Sequence LengthMass (Da)Tools
P50462 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: E35CF30CA17CB227

FASTA19420,895
        10         20         30         40         50         60 
MPNWGGGAKC GACEKTVYHA EEIQCNGRSF HKTCFHCMAC RKALDSTTVA AHESEIYCKV 

        70         80         90        100        110        120 
CYGRRYGPKG IGFGQGAGCL STDTGEHLGL QFQQSPKPAR AATTSNPSKF SAKFGESEKC 

       130        140        150        160        170        180 
PRCGKSVYAA EKVMGGGKPW HKTCFRCAIC GKSLESTNVT DKDGELYCKV CYAKNFGPTG 

       190 
IGFGGLTQQV EKKE 

« Hide

References

« Hide 'large scale' references
[1]"Murine MLP: cloning and expression in the embryonic head."
Harrod G.V., Kettunen P.J., Jowett A.K.
J. Craniofac. Genet. Dev. Biol. 16:65-73(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: MF1.
Tissue: Mandible.
[2]Hashimoto N., Ogashiwa M.
Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C3H.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[4]"MLP-deficient mice exhibit a disruption of cardiac cytoarchitectural organization, dilated cardiomyopathy, and heart failure."
Arber S., Hunter J.J., Ross J. Jr., Hongo M., Sansig G., Borg J., Perriard J.C., Chien K.R., Caroni P.
Cell 88:393-403(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
Strain: 129/Sv.
[5]"Attenuation of cardiac remodeling after myocardial infarction by muscle LIM protein-calcineurin signaling at the sarcomeric Z-disc."
Heineke J., Ruetten H., Willenbockel C., Gross S.C., Naguib M., Schaefer A., Kempf T., Hilfiker-Kleiner D., Caroni P., Kraft T., Kaiser R.A., Molkentin J.D., Drexler H., Wollert K.C.
Proc. Natl. Acad. Sci. U.S.A. 102:1655-1660(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]"PICOT attenuates cardiac hypertrophy by disrupting calcineurin-NFAT signaling."
Jeong D., Kim J.M., Cha H., Oh J.G., Park J., Yun S.H., Ju E.S., Jeon E.S., Hajjar R.J., Park W.J.
Circ. Res. 102:711-719(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GLRX3, SUBCELLULAR LOCATION.
[7]"A common MLP (muscle LIM protein) variant is associated with cardiomyopathy."
Knoll R., Kostin S., Klede S., Savvatis K., Klinge L., Stehle I., Gunkel S., Kotter S., Babicz K., Sohns M., Miocic S., Didie M., Knoll G., Zimmermann W.H., Thelen P., Bickeboller H., Maier L.S., Schaper W. expand/collapse author list , Schaper J., Kraft T., Tschope C., Linke W.A., Chien K.R.
Circ. Res. 106:695-704(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF TRP-4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49883 mRNA. Translation: CAA90039.1.
D88791 mRNA. Translation: BAA13721.1.
BC061131 mRNA. Translation: AAH61131.1.
PIRS57472.
RefSeqNP_001185770.1. NM_001198841.1.
NP_038836.1. NM_013808.4.
XP_006540658.1. XM_006540595.1.
UniGeneMm.17235.
Mm.488132.

3D structure databases

ProteinModelPortalP50462.
SMRP50462. Positions 1-178.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP50462. 2 interactions.
MINTMINT-4092342.
STRING10090.ENSMUSP00000032658.

PTM databases

PhosphoSiteP50462.

Proteomic databases

PaxDbP50462.
PRIDEP50462.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032658; ENSMUSP00000032658; ENSMUSG00000030470.
ENSMUST00000167786; ENSMUSP00000129378; ENSMUSG00000030470.
GeneID13009.
KEGGmmu:13009.
UCSCuc009hax.2. mouse.

Organism-specific databases

CTD8048.
MGIMGI:1330824. Csrp3.

Phylogenomic databases

eggNOGNOG294681.
HOGENOMHOG000111233.
HOVERGENHBG051143.
InParanoidP50462.
KOK09377.
OMAKYGPKGI.
OrthoDBEOG7CK39M.
PhylomeDBP50462.
TreeFamTF313758.

Gene expression databases

ArrayExpressP50462.
BgeeP50462.
CleanExMM_CSRP3.
GenevestigatorP50462.

Family and domain databases

Gene3D2.10.110.10. 2 hits.
InterProIPR001781. Znf_LIM.
[Graphical view]
PfamPF00412. LIM. 2 hits.
[Graphical view]
SMARTSM00132. LIM. 2 hits.
[Graphical view]
PROSITEPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio282844.
PROP50462.
SOURCESearch...

Entry information

Entry nameCSRP3_MOUSE
AccessionPrimary (citable) accession number: P50462
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot