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P50462

- CSRP3_MOUSE

UniProt

P50462 - CSRP3_MOUSE

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Protein
Cysteine and glycine-rich protein 3
Gene
Csrp3, Clp, Mlp
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Positive regulator of myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation.2 Publications

GO - Molecular functioni

  1. actinin binding Source: BHF-UCL
  2. protein binding Source: UniProtKB
  3. structural constituent of muscle Source: BHF-UCL
  4. telethonin binding Source: BHF-UCL
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. cardiac muscle contraction Source: BHF-UCL
  2. cardiac muscle hypertrophy Source: Ensembl
  3. cardiac muscle tissue development Source: MGI
  4. cardiac myofibril assembly Source: BHF-UCL
  5. cellular calcium ion homeostasis Source: MGI
  6. detection of muscle stretch Source: BHF-UCL
  7. muscle organ development Source: UniProtKB-KW
  8. protein localization to organelle Source: BHF-UCL
  9. regulation of the force of heart contraction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Myogenesis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine and glycine-rich protein 3
Alternative name(s):
Cysteine-rich protein 3
Short name:
CRP3
LIM domain protein, cardiac
Muscle LIM protein
Gene namesi
Name:Csrp3
Synonyms:Clp, Mlp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1330824. Csrp3.

Subcellular locationi

Nucleus Reviewed prediction. Cytoplasm. Cytoplasmcytoskeleton Reviewed prediction. CytoplasmmyofibrilsarcomereZ line
Note: Mainly cytoplasmic. In the nucleus it associates with the actin cytoskeleton Reviewed prediction. In the Z line, found associated with GLRX3 (via C-terminus).1 Publication

GO - Cellular componenti

  1. Z disc Source: MGI
  2. cytoskeleton Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant mice developed dilated cardiomyopathy with hypertrophy and heart failure after birth. Ultrastructural analysis revealed a dramatic disruption of cardiomyocyte cytoarchitecture. At birth, these hearts are not hypertrophic, but already abnormally soft, with cell-autonomous and Csrp3-sensitive alterations in cytoarchitecture. The morphological, functional, and molecular features of the cardiac phenotype in mutant adult mice are undistinguishable from those seen in human heart failure resulting from dilated cardiomyopathy of various etiolologies, these mice can thus be used as model. Heterozygous mice display a more pronounced left ventricular dilation and systolic dysfunction and decreased survival after myocardial infaction.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi4 – 41W → R: Mice develop an age- and gene dosage-dependent hypertrophic cardiomyopathy and heart failure phenotype, characterized by almost complete loss of contractile reserve under catecholamine induced stress. They display increased in septum wall thickness, fractional shortening, and wall thickness per diameter (h/r). There is also evidence for skeletal muscle pathology. In addition, homozygous mutants show increased left ventricle (LC) mass per body weight (BW) and significantly reduced body weight. An increased nuclear localization of Csrp3 is also observed. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 194194Cysteine and glycine-rich protein 3
PRO_0000075728Add
BLAST

Proteomic databases

MaxQBiP50462.
PaxDbiP50462.
PRIDEiP50462.

PTM databases

PhosphoSiteiP50462.

Expressioni

Gene expression databases

ArrayExpressiP50462.
BgeeiP50462.
CleanExiMM_CSRP3.
GenevestigatoriP50462.

Interactioni

Subunit structurei

Interacts with LDHD By similarity. Interacts with GLRX3.1 Publication

Protein-protein interaction databases

IntActiP50462. 2 interactions.
MINTiMINT-4092342.
STRINGi10090.ENSMUSP00000032658.

Structurei

3D structure databases

ProteinModelPortaliP50462.
SMRiP50462. Positions 1-178.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 6152LIM zinc-binding 1
Add
BLAST
Domaini120 – 17152LIM zinc-binding 2
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 696Nuclear localization signal Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi63 – 7816Gly-rich
Add
BLAST
Compositional biasi177 – 1859Gly-rich

Sequence similaritiesi

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiNOG294681.
HOGENOMiHOG000111233.
HOVERGENiHBG051143.
InParanoidiP50462.
KOiK09377.
OMAiQSPKQAR.
OrthoDBiEOG7CK39M.
PhylomeDBiP50462.
TreeFamiTF313758.

Family and domain databases

Gene3Di2.10.110.10. 2 hits.
InterProiIPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 2 hits.
[Graphical view]
SMARTiSM00132. LIM. 2 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50462-1 [UniParc]FASTAAdd to Basket

« Hide

MPNWGGGAKC GACEKTVYHA EEIQCNGRSF HKTCFHCMAC RKALDSTTVA    50
AHESEIYCKV CYGRRYGPKG IGFGQGAGCL STDTGEHLGL QFQQSPKPAR 100
AATTSNPSKF SAKFGESEKC PRCGKSVYAA EKVMGGGKPW HKTCFRCAIC 150
GKSLESTNVT DKDGELYCKV CYAKNFGPTG IGFGGLTQQV EKKE 194
Length:194
Mass (Da):20,895
Last modified:October 1, 1996 - v1
Checksum:iE35CF30CA17CB227
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49883 mRNA. Translation: CAA90039.1.
D88791 mRNA. Translation: BAA13721.1.
BC061131 mRNA. Translation: AAH61131.1.
CCDSiCCDS21305.1.
PIRiS57472.
RefSeqiNP_001185770.1. NM_001198841.1.
NP_038836.1. NM_013808.4.
XP_006540658.1. XM_006540595.1.
UniGeneiMm.17235.
Mm.488132.

Genome annotation databases

EnsembliENSMUST00000032658; ENSMUSP00000032658; ENSMUSG00000030470.
ENSMUST00000167786; ENSMUSP00000129378; ENSMUSG00000030470.
GeneIDi13009.
KEGGimmu:13009.
UCSCiuc009hax.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49883 mRNA. Translation: CAA90039.1 .
D88791 mRNA. Translation: BAA13721.1 .
BC061131 mRNA. Translation: AAH61131.1 .
CCDSi CCDS21305.1.
PIRi S57472.
RefSeqi NP_001185770.1. NM_001198841.1.
NP_038836.1. NM_013808.4.
XP_006540658.1. XM_006540595.1.
UniGenei Mm.17235.
Mm.488132.

3D structure databases

ProteinModelPortali P50462.
SMRi P50462. Positions 1-178.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P50462. 2 interactions.
MINTi MINT-4092342.
STRINGi 10090.ENSMUSP00000032658.

PTM databases

PhosphoSitei P50462.

Proteomic databases

MaxQBi P50462.
PaxDbi P50462.
PRIDEi P50462.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000032658 ; ENSMUSP00000032658 ; ENSMUSG00000030470 .
ENSMUST00000167786 ; ENSMUSP00000129378 ; ENSMUSG00000030470 .
GeneIDi 13009.
KEGGi mmu:13009.
UCSCi uc009hax.2. mouse.

Organism-specific databases

CTDi 8048.
MGIi MGI:1330824. Csrp3.

Phylogenomic databases

eggNOGi NOG294681.
HOGENOMi HOG000111233.
HOVERGENi HBG051143.
InParanoidi P50462.
KOi K09377.
OMAi QSPKQAR.
OrthoDBi EOG7CK39M.
PhylomeDBi P50462.
TreeFami TF313758.

Miscellaneous databases

NextBioi 282844.
PROi P50462.
SOURCEi Search...

Gene expression databases

ArrayExpressi P50462.
Bgeei P50462.
CleanExi MM_CSRP3.
Genevestigatori P50462.

Family and domain databases

Gene3Di 2.10.110.10. 2 hits.
InterProi IPR001781. Znf_LIM.
[Graphical view ]
Pfami PF00412. LIM. 2 hits.
[Graphical view ]
SMARTi SM00132. LIM. 2 hits.
[Graphical view ]
PROSITEi PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Murine MLP: cloning and expression in the embryonic head."
    Harrod G.V., Kettunen P.J., Jowett A.K.
    J. Craniofac. Genet. Dev. Biol. 16:65-73(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: MF1.
    Tissue: Mandible.
  2. Hashimoto N., Ogashiwa M.
    Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  4. "MLP-deficient mice exhibit a disruption of cardiac cytoarchitectural organization, dilated cardiomyopathy, and heart failure."
    Arber S., Hunter J.J., Ross J. Jr., Hongo M., Sansig G., Borg J., Perriard J.C., Chien K.R., Caroni P.
    Cell 88:393-403(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: 129/Sv.
  5. "Attenuation of cardiac remodeling after myocardial infarction by muscle LIM protein-calcineurin signaling at the sarcomeric Z-disc."
    Heineke J., Ruetten H., Willenbockel C., Gross S.C., Naguib M., Schaefer A., Kempf T., Hilfiker-Kleiner D., Caroni P., Kraft T., Kaiser R.A., Molkentin J.D., Drexler H., Wollert K.C.
    Proc. Natl. Acad. Sci. U.S.A. 102:1655-1660(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "PICOT attenuates cardiac hypertrophy by disrupting calcineurin-NFAT signaling."
    Jeong D., Kim J.M., Cha H., Oh J.G., Park J., Yun S.H., Ju E.S., Jeon E.S., Hajjar R.J., Park W.J.
    Circ. Res. 102:711-719(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GLRX3, SUBCELLULAR LOCATION.
  7. Cited for: MUTAGENESIS OF TRP-4.

Entry informationi

Entry nameiCSRP3_MOUSE
AccessioniPrimary (citable) accession number: P50462
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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