ID   LHX2_HUMAN              Reviewed;         406 AA.
AC   P50458; O95860; Q52M57; Q8N1Z3;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   27-MAR-2024, entry version 196.
DE   RecName: Full=LIM/homeobox protein Lhx2;
DE            Short=Homeobox protein LH-2;
DE            Short=LIM homeobox protein 2;
GN   Name=LHX2; Synonyms=LH2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Placenta;
RX   PubMed=8649822;
RA   Wu H.-K., Heng H.H.Q., Siderovski D.P., Dong W.-F., Okuno Y., Shi X.-M.,
RA   Tsui L.-C., Minden M.D.;
RT   "Identification of a human LIM-Hox gene, hLH-2, aberrantly expressed in
RT   chronic myelogenous leukaemia and located on 9q33-34.1.";
RL   Oncogene 12:1205-1212(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-406.
RC   TISSUE=Brain cortex;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 18-406.
RX   PubMed=10051612; DOI=10.1073/pnas.96.5.2165;
RA   Rincon-Limas D.E., Lu C.-H., Canal I., Calleja M., Rodriguez-Esteban C.,
RA   Izpisua-Belmonte J.-C., Botas J.;
RT   "Conservation of the expression and function of apterous orthologs in
RT   Drosophila and mammals.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:2165-2170(1999).
CC   -!- FUNCTION: Acts as a transcriptional activator. Stimulates the promoter
CC       of the alpha-glycoprotein gene. Transcriptional regulatory protein
CC       involved in the control of cell differentiation in developing lymphoid
CC       and neural cell types (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts (via LIM domains) with CITED2. Interacts with
CC       POU4F2. {ECO:0000250|UniProtKB:Q9Z0S2}.
CC   -!- INTERACTION:
CC       P50458; Q49AR9: ANKS1A; NbExp=3; IntAct=EBI-12179869, EBI-11954519;
CC       P50458; A7KAX9: ARHGAP32; NbExp=3; IntAct=EBI-12179869, EBI-308663;
CC       P50458; Q8NDZ0: BEND2; NbExp=3; IntAct=EBI-12179869, EBI-954079;
CC       P50458; Q4VC44: FLYWCH1; NbExp=3; IntAct=EBI-12179869, EBI-719415;
CC       P50458; Q9NSC5: HOMER3; NbExp=3; IntAct=EBI-12179869, EBI-748420;
CC       P50458; P31273: HOXC8; NbExp=3; IntAct=EBI-12179869, EBI-1752118;
CC       P50458; P50053-2: KHK; NbExp=3; IntAct=EBI-12179869, EBI-12204387;
CC       P50458; Q86U70: LDB1; NbExp=3; IntAct=EBI-12179869, EBI-677177;
CC       P50458; Q86U70-2: LDB1; NbExp=3; IntAct=EBI-12179869, EBI-11979761;
CC       P50458; Q3ZCW2: LGALSL; NbExp=3; IntAct=EBI-12179869, EBI-10241423;
CC       P50458; Q8TBB1: LNX1; NbExp=3; IntAct=EBI-12179869, EBI-739832;
CC       P50458; Q17RB8: LONRF1; NbExp=3; IntAct=EBI-12179869, EBI-2341787;
CC       P50458; Q9UBU8-2: MORF4L1; NbExp=3; IntAct=EBI-12179869, EBI-10288852;
CC       P50458; Q6PF18: MORN3; NbExp=3; IntAct=EBI-12179869, EBI-9675802;
CC       P50458; Q99650: OSMR; NbExp=3; IntAct=EBI-12179869, EBI-2804080;
CC       P50458; Q12837: POU4F2; NbExp=3; IntAct=EBI-12179869, EBI-17236143;
CC       P50458; Q9Y3C6: PPIL1; NbExp=3; IntAct=EBI-12179869, EBI-2557649;
CC       P50458; Q8N8B7-2: TCEANC; NbExp=3; IntAct=EBI-12179869, EBI-11955057;
CC       P50458; Q9NUJ3: TCP11L1; NbExp=3; IntAct=EBI-12179869, EBI-2555179;
CC       P50458; Q9UKI8: TLK1; NbExp=3; IntAct=EBI-12179869, EBI-740492;
CC       P50458; Q9UL33-2: TRAPPC2L; NbExp=3; IntAct=EBI-12179869, EBI-11119202;
CC       P50458; P0CB47: UBTFL1; NbExp=3; IntAct=EBI-12179869, EBI-17208936;
CC       P50458; Q96MM3: ZFP42; NbExp=3; IntAct=EBI-12179869, EBI-12151755;
CC       P50458; Q8NB15: ZNF511; NbExp=3; IntAct=EBI-12179869, EBI-10269136;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- DOMAIN: LIM domains are necessary for transcription activation.
CC       {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAC04371.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U11701; AAB08752.1; -; mRNA.
DR   EMBL; BC093662; AAH93662.1; -; mRNA.
DR   EMBL; BC112185; AAI12186.1; -; mRNA.
DR   EMBL; AK094511; BAC04371.1; ALT_INIT; mRNA.
DR   EMBL; AF124735; AAD20013.1; -; mRNA.
DR   CCDS; CCDS6853.1; -.
DR   RefSeq; NP_004780.3; NM_004789.3.
DR   AlphaFoldDB; P50458; -.
DR   BMRB; P50458; -.
DR   SMR; P50458; -.
DR   BioGRID; 114758; 177.
DR   IntAct; P50458; 171.
DR   MINT; P50458; -.
DR   STRING; 9606.ENSP00000362717; -.
DR   iPTMnet; P50458; -.
DR   PhosphoSitePlus; P50458; -.
DR   BioMuta; LHX2; -.
DR   DMDM; 8247936; -.
DR   jPOST; P50458; -.
DR   MassIVE; P50458; -.
DR   PaxDb; 9606-ENSP00000362717; -.
DR   PeptideAtlas; P50458; -.
DR   ProteomicsDB; 56230; -.
DR   Antibodypedia; 16286; 460 antibodies from 35 providers.
DR   DNASU; 9355; -.
DR   Ensembl; ENST00000373615.9; ENSP00000362717.4; ENSG00000106689.11.
DR   GeneID; 9355; -.
DR   KEGG; hsa:9355; -.
DR   MANE-Select; ENST00000373615.9; ENSP00000362717.4; NM_004789.4; NP_004780.3.
DR   UCSC; uc004boe.2; human.
DR   AGR; HGNC:6594; -.
DR   CTD; 9355; -.
DR   DisGeNET; 9355; -.
DR   GeneCards; LHX2; -.
DR   HGNC; HGNC:6594; LHX2.
DR   HPA; ENSG00000106689; Group enriched (brain, retina).
DR   MIM; 603759; gene.
DR   neXtProt; NX_P50458; -.
DR   OpenTargets; ENSG00000106689; -.
DR   PharmGKB; PA30365; -.
DR   VEuPathDB; HostDB:ENSG00000106689; -.
DR   eggNOG; KOG0490; Eukaryota.
DR   GeneTree; ENSGT00940000158540; -.
DR   HOGENOM; CLU_027802_4_1_1; -.
DR   InParanoid; P50458; -.
DR   OMA; AIFHLEC; -.
DR   OrthoDB; 2880952at2759; -.
DR   PhylomeDB; P50458; -.
DR   TreeFam; TF315442; -.
DR   PathwayCommons; P50458; -.
DR   Reactome; R-HSA-9010553; Regulation of expression of SLITs and ROBOs.
DR   Reactome; R-HSA-9752946; Expression and translocation of olfactory receptors.
DR   SignaLink; P50458; -.
DR   SIGNOR; P50458; -.
DR   BioGRID-ORCS; 9355; 7 hits in 1170 CRISPR screens.
DR   ChiTaRS; LHX2; human.
DR   GeneWiki; LHX2; -.
DR   GenomeRNAi; 9355; -.
DR   Pharos; P50458; Tbio.
DR   PRO; PR:P50458; -.
DR   Proteomes; UP000005640; Chromosome 9.
DR   RNAct; P50458; Protein.
DR   Bgee; ENSG00000106689; Expressed in ventricular zone and 119 other cell types or tissues.
DR   ExpressionAtlas; P50458; baseline and differential.
DR   GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003682; F:chromatin binding; IEA:Ensembl.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:Ensembl.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IEA:Ensembl.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0048675; P:axon extension; IEA:Ensembl.
DR   GO; GO:0007411; P:axon guidance; IEA:Ensembl.
DR   GO; GO:0021987; P:cerebral cortex development; IEA:Ensembl.
DR   GO; GO:0009953; P:dorsal/ventral pattern formation; IEA:Ensembl.
DR   GO; GO:0001942; P:hair follicle development; IEA:Ensembl.
DR   GO; GO:0045199; P:maintenance of epithelial cell apical/basal polarity; IEA:Ensembl.
DR   GO; GO:0007498; P:mesoderm development; IEA:Ensembl.
DR   GO; GO:0045814; P:negative regulation of gene expression, epigenetic; IEA:Ensembl.
DR   GO; GO:0050768; P:negative regulation of neurogenesis; IEA:Ensembl.
DR   GO; GO:0001843; P:neural tube closure; IEA:Ensembl.
DR   GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR   GO; GO:0021772; P:olfactory bulb development; IEA:Ensembl.
DR   GO; GO:0045893; P:positive regulation of DNA-templated transcription; ISS:UniProtKB.
DR   GO; GO:2000179; P:positive regulation of neural precursor cell proliferation; IEA:Ensembl.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0060041; P:retina development in camera-type eye; IEA:Ensembl.
DR   GO; GO:0021978; P:telencephalon regionalization; IEA:Ensembl.
DR   CDD; cd00086; homeodomain; 1.
DR   CDD; cd09469; LIM1_Lhx2; 1.
DR   CDD; cd09377; LIM2_Lhx2_Lhx9; 1.
DR   Gene3D; 2.10.110.10; Cysteine Rich Protein; 2.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR001781; Znf_LIM.
DR   PANTHER; PTHR24208; LIM/HOMEOBOX PROTEIN LHX; 1.
DR   PANTHER; PTHR24208:SF80; LIM_HOMEOBOX PROTEIN LHX2; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00412; LIM; 2.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00132; LIM; 2.
DR   SUPFAM; SSF57716; Glucocorticoid receptor-like (DNA-binding domain); 2.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR   PROSITE; PS50023; LIM_DOMAIN_2; 2.
DR   Genevisible; P50458; HS.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Homeobox; LIM domain; Metal-binding; Nucleus;
KW   Reference proteome; Repeat; Transcription; Transcription regulation; Zinc.
FT   CHAIN           1..406
FT                   /note="LIM/homeobox protein Lhx2"
FT                   /id="PRO_0000075778"
FT   DOMAIN          53..105
FT                   /note="LIM zinc-binding 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DOMAIN          115..168
FT                   /note="LIM zinc-binding 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DNA_BIND        266..325
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          250..270
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          328..374
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          387..406
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           307..323
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        330..374
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        22..28
FT                   /note="AKSEAPA -> QERGSR (in Ref. 1; AAB08752)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        54
FT                   /note="A -> G (in Ref. 1; AAB08752)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        107
FT                   /note="Y -> YSPSLHGPY (in Ref. 3)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        187
FT                   /note="A -> Q (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        190..192
FT                   /note="AAA -> RAR (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        196
FT                   /note="Missing (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        241..242
FT                   /note="NA -> TR (in Ref. 1; AAB08752)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        391..406
FT                   /note="GHEPHSPSQTTLTNLF -> AMSLTAPHKRLLPTFSNDSQPPHPTISLKKKL
FT                   SLV (in Ref. 1; AAB08752)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   406 AA;  44373 MW;  DF73AAC81867D30D CRC64;
     MLFHSLSGPE VHGVIDEMDR RAKSEAPAIS SAIDRGDTET TMPSISSDRA ALCAGCGGKI
     SDRYYLLAVD KQWHMRCLKC CECKLNLESE LTCFSKDGSI YCKEDYYRRF SVQRCARCHL
     GISASEMVMR ARDLVYHLNC FTCTTCNKML TTGDHFGMKD SLVYCRLHFE ALLQGEYPAH
     FNHADVAAAA AAAAAAKSAG LGAAGANPLG LPYYNGVGTV QKGRPRKRKS PGPGADLAAY
     NAALSCNEND AEHLDRDQPY PSSQKTKRMR TSFKHHQLRT MKSYFAINHN PDAKDLKQLA
     QKTGLTKRVL QVWFQNARAK FRRNLLRQEN TGVDKSTDAA LQTGTPSGPA SELSNASLSP
     SSTPTTLTDL TSPTLPTVTS VLTSVPGNLE GHEPHSPSQT TLTNLF
//