Reviewed, UniProtKB/Swiss-Prot P50457 (PUUE_ECOLI)

Names and origin

Protein names

Recommended name:
    4-aminobutyrate aminotransferase
    EC=2.6.1.19
Alternative name(s):
    Gamma-amino-N-butyrate transaminase
      Short name=GABA transaminase
    GABA aminotransferase
      Short name=GABA-AT
    Glutamate:succinic semialdehyde transaminase

Gene names

Name:	puuE
Synonyms:	goaG
Ordered Locus Names:	b1302, JW1295

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate. Ref.5
Catalytic activity	4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.
Cofactor	Pyridoxal phosphate.
Pathway	Amine and polyamine degradation; putrescine degradation; succinate semialdehyde from 4-aminobutanoate: step 1/1.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gamma-aminobutyric acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-aminobutyrate transaminase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	4-aminobutyrate aminotransferase		PRO_0000120388
Amino acid modifications
	Modified residue	267	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50457-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A2C17A885FEBE4EB	FASTA	421	44,729
	10 20 30 40 50 60 MSNNEFHQRR LSATPRGVGV MCNFFAQSAE NATLKDVEGN EYIDFAAGIA VLNTGHRHPD 70 80 90 100 110 120 LVAAVEQQLQ QFTHTAYQIV PYESYVTLAE KINALAPVSG QAKTAFFTTG AEAVENAVKI 130 140 150 160 170 180 ARAHTGRPGV IAFSGGFHGR TYMTMALTGK VAPYKIGFGP FPGSVYHVPY PSDLHGISTQ 190 200 210 220 230 240 DSLDAIERLF KSDIEAKQVA AIIFEPVQGE GGFNVAPKEL VAAIRRLCDE HGIVMIADEV 250 260 270 280 290 300 QSGFARTGKL FAMDHYADKP DLMTMAKSLA GGMPLSGVVG NANIMDAPAP GGLGGTYAGN 310 320 330 340 350 360 PLAVAAAHAV LNIIDKESLC ERANQLGQRL KNTLIDAKES VPAIAAVRGL GSMIAVEFND 370 380 390 400 410 420 PQTGEPSAAI AQKIQQRALA QGLLLLTCGA YGNVIRFLYP LTIPDAQFDA AMKILQDALS D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Jovanovic G. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"A novel putrescine utilization pathway involves gamma-glutamylated intermediates of Escherichia coli K-12." Kurihara S., Oda S., Kato K., Kim H.G., Koyanagi T., Kumagai H., Suzuki H. J. Biol. Chem. 280:4602-4608(2005) [PubMed: 15590624] [Abstract] Cited for: FUNCTION. Strain: K12.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U38543 Genomic DNA. Translation: AAC45301.1. U00096 Genomic DNA. Translation: AAC74384.1. AP009048 Genomic DNA. Translation: BAA14871.1.
PIR	A64879.
RefSeq	AP_001928.1. NP_415818.1.
3D structure databases
HSSP	HSSP built from PDB template 1QJ3 based on UniProtKB P12995.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9825N.
STRING	P50457.
Genome annotation databases
GeneID	945446.
GenomeReviews	Gene locus JW1295 in contig AP009048_GR. Gene locus b1302 in contig U00096_GR.
KEGG	ecj:JW1295. eco:b1302.
Organism-specific databases
EchoBASE	EB2979.
EcoGene	EG13187. puuE.
CMR	Search...
Phylogenomic databases
HOGENOM	P50457.
OMA	MCDFYAV.
Enzyme and pathway databases
BioCyc	EcoCyc:G6646-MON. MetaCyc:G6646-MON.
Gene expression databases
Genevestigator	P50457.
Family and domain databases
InterPro	IPR004632. 4NH2But_aminotransferase_bac. IPR005814. Aminotrans_3. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11986. Aminotrans_3. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00700. GABAtrnsam. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUUE_ECOLI

Accession

Primary (citable) accession number: P50457
Secondary accession number(s): P78150

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)