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P50457

- PUUE_ECOLI

UniProt

P50457 - PUUE_ECOLI

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Protein
4-aminobutyrate aminotransferase PuuE
Gene
puuE, goaG, b1302, JW1295
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the amino group from gamma-aminobutyrate (GABA) to alpha-ketoglutarate (KG) to yield succinic semialdehyde (SSA). PuuE is important for utilization of putrescine as the sole nitrogen or carbon source.2 Publications

Catalytic activityi

4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.

Cofactori

Pyridoxal phosphate.

Enzyme regulationi

Completely inhibited by succinate and low-aeration conditions.1 Publication

Kineticsi

  1. KM=0.0577 mM for SSA (at pH 7.8 and at 30 degrees Celsius)1 Publication
  2. KM=1.57 mM for GABA (at pH 7.8 and at 30 degrees Celsius)
  3. KM=5.1 mM for KG (at pH 7.8 and at 30 degrees Celsius)
  4. KM=18 mM for L-glutamate (at pH 7.8 and at 30 degrees Celsius)

pH dependencei

Optimum pH is 9 for the forward reaction which is the transfer reaction of the amino group from GABA to beta-ketoglutarate, and pH 8 for the reverse reaction.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei296 – 2961Pyridoxal phosphate By similarity

GO - Molecular functioni

  1. 4-aminobutyrate transaminase activity Source: EcoCyc
  2. pyridoxal phosphate binding Source: EcoCyc
Complete GO annotation...

GO - Biological processi

  1. gamma-aminobutyric acid metabolic process Source: InterPro
  2. putrescine catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:G6646-MONOMER.
ECOL316407:JW1295-MONOMER.
MetaCyc:G6646-MONOMER.
SABIO-RKP50457.
UniPathwayiUPA00188; UER00293.

Names & Taxonomyi

Protein namesi
Recommended name:
4-aminobutyrate aminotransferase PuuE (EC:2.6.1.19)
Alternative name(s):
GABA aminotransferase
Short name:
GABA-AT
Gamma-amino-N-butyrate transaminase
Short name:
GABA transaminase
Glutamate:succinic semialdehyde transaminase
Gene namesi
Name:puuE
Synonyms:goaG
Ordered Locus Names:b1302, JW1295
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13187. puuE.

Pathology & Biotechi

Disruption phenotypei

Cells show only 35% of the wild-type activity.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi267 – 2671K → A: No GABA-AT activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4214214-aminobutyrate aminotransferase PuuE
PRO_0000120388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei267 – 2671N6-(pyridoxal phosphate)lysine By similarity

Proteomic databases

PaxDbiP50457.
PRIDEiP50457.

Expressioni

Inductioni

By putrescine.1 Publication

Gene expression databases

GenevestigatoriP50457.

Interactioni

Protein-protein interaction databases

DIPiDIP-9825N.
IntActiP50457. 3 interactions.
MINTiMINT-1256713.
STRINGi511145.b1302.

Structurei

3D structure databases

ProteinModelPortaliP50457.
SMRiP50457. Positions 2-421.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni110 – 1112Pyridoxal phosphate binding By similarity
Regioni238 – 2414Pyridoxal phosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0160.
HOGENOMiHOG000020206.
KOiK00823.
OMAiMCGFYAE.
OrthoDBiEOG6QVRHN.
PhylomeDBiP50457.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004632. 4NH2But_aminotransferase_bac.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00700. GABAtrnsam. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50457-1 [UniParc]FASTAAdd to Basket

« Hide

MSNNEFHQRR LSATPRGVGV MCNFFAQSAE NATLKDVEGN EYIDFAAGIA    50
VLNTGHRHPD LVAAVEQQLQ QFTHTAYQIV PYESYVTLAE KINALAPVSG 100
QAKTAFFTTG AEAVENAVKI ARAHTGRPGV IAFSGGFHGR TYMTMALTGK 150
VAPYKIGFGP FPGSVYHVPY PSDLHGISTQ DSLDAIERLF KSDIEAKQVA 200
AIIFEPVQGE GGFNVAPKEL VAAIRRLCDE HGIVMIADEV QSGFARTGKL 250
FAMDHYADKP DLMTMAKSLA GGMPLSGVVG NANIMDAPAP GGLGGTYAGN 300
PLAVAAAHAV LNIIDKESLC ERANQLGQRL KNTLIDAKES VPAIAAVRGL 350
GSMIAVEFND PQTGEPSAAI AQKIQQRALA QGLLLLTCGA YGNVIRFLYP 400
LTIPDAQFDA AMKILQDALS D 421
Length:421
Mass (Da):44,729
Last modified:October 1, 1996 - v1
Checksum:iA2C17A885FEBE4EB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U38543 Genomic DNA. Translation: AAC45301.1.
U00096 Genomic DNA. Translation: AAC74384.1.
AP009048 Genomic DNA. Translation: BAA14871.1.
PIRiA64879.
RefSeqiNP_415818.1. NC_000913.3.
YP_489570.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74384; AAC74384; b1302.
BAA14871; BAA14871; BAA14871.
GeneIDi12934532.
945446.
KEGGiecj:Y75_p1277.
eco:b1302.
PATRICi32117876. VBIEscCol129921_1358.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U38543 Genomic DNA. Translation: AAC45301.1 .
U00096 Genomic DNA. Translation: AAC74384.1 .
AP009048 Genomic DNA. Translation: BAA14871.1 .
PIRi A64879.
RefSeqi NP_415818.1. NC_000913.3.
YP_489570.1. NC_007779.1.

3D structure databases

ProteinModelPortali P50457.
SMRi P50457. Positions 2-421.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-9825N.
IntActi P50457. 3 interactions.
MINTi MINT-1256713.
STRINGi 511145.b1302.

Proteomic databases

PaxDbi P50457.
PRIDEi P50457.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74384 ; AAC74384 ; b1302 .
BAA14871 ; BAA14871 ; BAA14871 .
GeneIDi 12934532.
945446.
KEGGi ecj:Y75_p1277.
eco:b1302.
PATRICi 32117876. VBIEscCol129921_1358.

Organism-specific databases

EchoBASEi EB2979.
EcoGenei EG13187. puuE.

Phylogenomic databases

eggNOGi COG0160.
HOGENOMi HOG000020206.
KOi K00823.
OMAi MCGFYAE.
OrthoDBi EOG6QVRHN.
PhylomeDBi P50457.

Enzyme and pathway databases

UniPathwayi UPA00188 ; UER00293 .
BioCyci EcoCyc:G6646-MONOMER.
ECOL316407:JW1295-MONOMER.
MetaCyc:G6646-MONOMER.
SABIO-RK P50457.

Miscellaneous databases

PROi P50457.

Gene expression databases

Genevestigatori P50457.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR004632. 4NH2But_aminotransferase_bac.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
PANTHERi PTHR11986. PTHR11986. 1 hit.
Pfami PF00202. Aminotran_3. 1 hit.
[Graphical view ]
PIRSFi PIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
TIGRFAMsi TIGR00700. GABAtrnsam. 1 hit.
PROSITEi PS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Jovanovic G.
    Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A novel putrescine utilization pathway involves gamma-glutamylated intermediates of Escherichia coli K-12."
    Kurihara S., Oda S., Kato K., Kim H.G., Koyanagi T., Kumagai H., Suzuki H.
    J. Biol. Chem. 280:4602-4608(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A GABA AMINOTRANSFERASE, NOMENCLATURE.
    Strain: K12.
  6. "A putrescine-inducible pathway comprising PuuE-YneI in which gamma-aminobutyrate is degraded into succinate in Escherichia coli K-12."
    Kurihara S., Kato K., Asada K., Kumagai H., Suzuki H.
    J. Bacteriol. 192:4582-4591(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PUTRESCINE DEGRADATION AND AS A GABA AMINOTRANSFERASE, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF LYS-267, DISRUPTION PHENOTYPE, ENZYME REGULATION, INDUCTION.

Entry informationi

Entry nameiPUUE_ECOLI
AccessioniPrimary (citable) accession number: P50457
Secondary accession number(s): P78150
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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