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Protein

Protein mlc

Gene

mlc

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi247Zinc1
Metal bindingi257Zinc1
Metal bindingi259Zinc1
Metal bindingi264Zinc1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi33 – 42H-T-H motifBy similarity10

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:PD01896.
ECOL316407:JW1586-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mlc
Alternative name(s):
Making large colonies protein
Gene namesi
Name:mlc
Ordered Locus Names:b1594, JW1586
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13156. mlc.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi257C → A or S: Strongly reduced activity. 1 Publication1
Mutagenesisi259C → A or S: Strongly reduced activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000956901 – 406Protein mlcAdd BLAST406

Proteomic databases

PaxDbiP50456.
PRIDEiP50456.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
mtfAP763462EBI-1116104,EBI-1126682

Protein-protein interaction databases

BioGridi4259119. 23 interactors.
DIPiDIP-10219N.
IntActiP50456. 9 interactors.
STRINGi511145.b1594.

Structurei

Secondary structure

1406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 26Combined sources14
Helixi33 – 39Combined sources7
Helixi44 – 56Combined sources13
Beta strandi59 – 62Combined sources4
Beta strandi78 – 80Combined sources3
Beta strandi86 – 93Combined sources8
Beta strandi96 – 103Combined sources8
Beta strandi108 – 115Combined sources8
Helixi124 – 138Combined sources15
Helixi140 – 142Combined sources3
Beta strandi146 – 158Combined sources13
Turni159 – 162Combined sources4
Beta strandi163 – 166Combined sources4
Beta strandi174 – 176Combined sources3
Helixi178 – 186Combined sources9
Beta strandi190 – 194Combined sources5
Helixi195 – 206Combined sources12
Turni208 – 211Combined sources4
Beta strandi213 – 230Combined sources18
Turni235 – 238Combined sources4
Helixi245 – 247Combined sources3
Beta strandi248 – 250Combined sources3
Beta strandi262 – 264Combined sources3
Helixi266 – 269Combined sources4
Helixi271 – 282Combined sources12
Helixi289 – 291Combined sources3
Helixi297 – 305Combined sources9
Helixi309 – 333Combined sources25
Beta strandi336 – 342Combined sources7
Helixi343 – 347Combined sources5
Helixi348 – 362Combined sources15
Helixi365 – 368Combined sources4
Beta strandi372 – 375Combined sources4
Turni382 – 385Combined sources4
Helixi386 – 393Combined sources8
Helixi398 – 403Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z6RX-ray2.70A/B/C/D1-406[»]
3BP8X-ray2.85A/B1-406[»]
ProteinModelPortaliP50456.
SMRiP50456.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50456.

Family & Domainsi

Sequence similaritiesi

Belongs to the ROK (NagC/XylR) family.Curated

Phylogenomic databases

eggNOGiENOG4105DAG. Bacteria.
COG1940. LUCA.
HOGENOMiHOG000275182.
InParanoidiP50456.
KOiK15545.
OMAiLIEAHLI.
PhylomeDBiP50456.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000600. ROK.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00480. ROK. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Complete.

P50456-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAENQPGHI DQIKQTNAGA VYRLIDQLGP VSRIDLSRLA QLAPASITKI
60 70 80 90 100
VREMLEAHLV QELEIKEAGN RGRPAVGLVV ETEAWHYLSL RISRGEIFLA
110 120 130 140 150
LRDLSSKLVV EESQELALKD DLPLLDRIIS HIDQFFIRHQ KKLERLTSIA
160 170 180 190 200
ITLPGIIDTE NGIVHRMPFY EDVKEMPLGE ALEQHTGVPV YIQHDISAWT
210 220 230 240 250
MAEALFGASR GARDVIQVVI DHNVGAGVIT DGHLLHAGSS SLVEIGHTQV
260 270 280 290 300
DPYGKRCYCG NHGCLETIAS VDSILELAQL RLNQSMSSML HGQPLTVDSL
310 320 330 340 350
CQAALRGDLL AKDIITGVGA HVGRILAIMV NLFNPQKILI GSPLSKAADI
360 370 380 390 400
LFPVISDSIR QQALPAYSQH ISVESTQFSN QGTMAGAALV KDAMYNGSLL

IRLLQG
Length:406
Mass (Da):44,316
Last modified:November 1, 1997 - v2
Checksum:i423608797FC980CB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti179G → A in BAA06978 (PubMed:7766024).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32222 Genomic DNA. Translation: BAA06978.1.
U00096 Genomic DNA. Translation: AAC74666.1.
AP009048 Genomic DNA. Translation: BAA15318.1.
PIRiD64915.
RefSeqiNP_416111.1. NC_000913.3.
WP_000225262.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74666; AAC74666; b1594.
BAA15318; BAA15318; BAA15318.
GeneIDi945510.
KEGGiecj:JW1586.
eco:b1594.
PATRICi32118490. VBIEscCol129921_1665.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32222 Genomic DNA. Translation: BAA06978.1.
U00096 Genomic DNA. Translation: AAC74666.1.
AP009048 Genomic DNA. Translation: BAA15318.1.
PIRiD64915.
RefSeqiNP_416111.1. NC_000913.3.
WP_000225262.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z6RX-ray2.70A/B/C/D1-406[»]
3BP8X-ray2.85A/B1-406[»]
ProteinModelPortaliP50456.
SMRiP50456.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259119. 23 interactors.
DIPiDIP-10219N.
IntActiP50456. 9 interactors.
STRINGi511145.b1594.

Proteomic databases

PaxDbiP50456.
PRIDEiP50456.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74666; AAC74666; b1594.
BAA15318; BAA15318; BAA15318.
GeneIDi945510.
KEGGiecj:JW1586.
eco:b1594.
PATRICi32118490. VBIEscCol129921_1665.

Organism-specific databases

EchoBASEiEB2950.
EcoGeneiEG13156. mlc.

Phylogenomic databases

eggNOGiENOG4105DAG. Bacteria.
COG1940. LUCA.
HOGENOMiHOG000275182.
InParanoidiP50456.
KOiK15545.
OMAiLIEAHLI.
PhylomeDBiP50456.

Enzyme and pathway databases

BioCyciEcoCyc:PD01896.
ECOL316407:JW1586-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP50456.
PROiP50456.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000600. ROK.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00480. ROK. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMLC_ECOLI
AccessioniPrimary (citable) accession number: P50456
Secondary accession number(s): P77593
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.