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Protein

Protein mlc

Gene

mlc

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi247 – 2471Zinc
Metal bindingi257 – 2571Zinc
Metal bindingi259 – 2591Zinc
Metal bindingi264 – 2641Zinc

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi33 – 4210H-T-H motifBy similarity

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:PD01896.
ECOL316407:JW1586-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mlc
Alternative name(s):
Making large colonies protein
Gene namesi
Name:mlc
Ordered Locus Names:b1594, JW1586
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13156. mlc.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi257 – 2571C → A or S: Strongly reduced activity. 1 Publication
Mutagenesisi259 – 2591C → A or S: Strongly reduced activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 406406Protein mlcPRO_0000095690Add
BLAST

Proteomic databases

PaxDbiP50456.
PRIDEiP50456.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
mtfAP763462EBI-1116104,EBI-1126682

Protein-protein interaction databases

BioGridi4259119. 23 interactions.
DIPiDIP-10219N.
IntActiP50456. 9 interactions.
STRINGi511145.b1594.

Structurei

Secondary structure

1
406
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 2614Combined sources
Helixi33 – 397Combined sources
Helixi44 – 5613Combined sources
Beta strandi59 – 624Combined sources
Beta strandi78 – 803Combined sources
Beta strandi86 – 938Combined sources
Beta strandi96 – 1038Combined sources
Beta strandi108 – 1158Combined sources
Helixi124 – 13815Combined sources
Helixi140 – 1423Combined sources
Beta strandi146 – 15813Combined sources
Turni159 – 1624Combined sources
Beta strandi163 – 1664Combined sources
Beta strandi174 – 1763Combined sources
Helixi178 – 1869Combined sources
Beta strandi190 – 1945Combined sources
Helixi195 – 20612Combined sources
Turni208 – 2114Combined sources
Beta strandi213 – 23018Combined sources
Turni235 – 2384Combined sources
Helixi245 – 2473Combined sources
Beta strandi248 – 2503Combined sources
Beta strandi262 – 2643Combined sources
Helixi266 – 2694Combined sources
Helixi271 – 28212Combined sources
Helixi289 – 2913Combined sources
Helixi297 – 3059Combined sources
Helixi309 – 33325Combined sources
Beta strandi336 – 3427Combined sources
Helixi343 – 3475Combined sources
Helixi348 – 36215Combined sources
Helixi365 – 3684Combined sources
Beta strandi372 – 3754Combined sources
Turni382 – 3854Combined sources
Helixi386 – 3938Combined sources
Helixi398 – 4036Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z6RX-ray2.70A/B/C/D1-406[»]
3BP8X-ray2.85A/B1-406[»]
ProteinModelPortaliP50456.
SMRiP50456. Positions 12-406.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50456.

Family & Domainsi

Sequence similaritiesi

Belongs to the ROK (NagC/XylR) family.Curated

Phylogenomic databases

eggNOGiENOG4105DAG. Bacteria.
COG1940. LUCA.
HOGENOMiHOG000275182.
InParanoidiP50456.
KOiK15545.
OMAiLIEAHLI.
PhylomeDBiP50456.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000600. ROK.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00480. ROK. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Complete.

P50456-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAENQPGHI DQIKQTNAGA VYRLIDQLGP VSRIDLSRLA QLAPASITKI
60 70 80 90 100
VREMLEAHLV QELEIKEAGN RGRPAVGLVV ETEAWHYLSL RISRGEIFLA
110 120 130 140 150
LRDLSSKLVV EESQELALKD DLPLLDRIIS HIDQFFIRHQ KKLERLTSIA
160 170 180 190 200
ITLPGIIDTE NGIVHRMPFY EDVKEMPLGE ALEQHTGVPV YIQHDISAWT
210 220 230 240 250
MAEALFGASR GARDVIQVVI DHNVGAGVIT DGHLLHAGSS SLVEIGHTQV
260 270 280 290 300
DPYGKRCYCG NHGCLETIAS VDSILELAQL RLNQSMSSML HGQPLTVDSL
310 320 330 340 350
CQAALRGDLL AKDIITGVGA HVGRILAIMV NLFNPQKILI GSPLSKAADI
360 370 380 390 400
LFPVISDSIR QQALPAYSQH ISVESTQFSN QGTMAGAALV KDAMYNGSLL

IRLLQG
Length:406
Mass (Da):44,316
Last modified:November 1, 1997 - v2
Checksum:i423608797FC980CB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti179 – 1791G → A in BAA06978 (PubMed:7766024).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32222 Genomic DNA. Translation: BAA06978.1.
U00096 Genomic DNA. Translation: AAC74666.1.
AP009048 Genomic DNA. Translation: BAA15318.1.
PIRiD64915.
RefSeqiNP_416111.1. NC_000913.3.
WP_000225262.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74666; AAC74666; b1594.
BAA15318; BAA15318; BAA15318.
GeneIDi945510.
KEGGiecj:JW1586.
eco:b1594.
PATRICi32118490. VBIEscCol129921_1665.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32222 Genomic DNA. Translation: BAA06978.1.
U00096 Genomic DNA. Translation: AAC74666.1.
AP009048 Genomic DNA. Translation: BAA15318.1.
PIRiD64915.
RefSeqiNP_416111.1. NC_000913.3.
WP_000225262.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z6RX-ray2.70A/B/C/D1-406[»]
3BP8X-ray2.85A/B1-406[»]
ProteinModelPortaliP50456.
SMRiP50456. Positions 12-406.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259119. 23 interactions.
DIPiDIP-10219N.
IntActiP50456. 9 interactions.
STRINGi511145.b1594.

Proteomic databases

PaxDbiP50456.
PRIDEiP50456.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74666; AAC74666; b1594.
BAA15318; BAA15318; BAA15318.
GeneIDi945510.
KEGGiecj:JW1586.
eco:b1594.
PATRICi32118490. VBIEscCol129921_1665.

Organism-specific databases

EchoBASEiEB2950.
EcoGeneiEG13156. mlc.

Phylogenomic databases

eggNOGiENOG4105DAG. Bacteria.
COG1940. LUCA.
HOGENOMiHOG000275182.
InParanoidiP50456.
KOiK15545.
OMAiLIEAHLI.
PhylomeDBiP50456.

Enzyme and pathway databases

BioCyciEcoCyc:PD01896.
ECOL316407:JW1586-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP50456.
PROiP50456.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000600. ROK.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00480. ROK. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMLC_ECOLI
AccessioniPrimary (citable) accession number: P50456
Secondary accession number(s): P77593
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.