Protein mlc - Escherichia coli (strain K12)

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P50456 (MLC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein mlc

Alternative name(s):
Making large colonies protein

Gene names

Name:	mlc
Ordered Locus Names:	b1594, JW1586

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT. Ref.5 Ref.6
Subunit structure	Homodimer. Ref.7
Sequence similarities	Belongs to the ROK (NagC/XylR) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Transcription Transcription regulation
Ligand	DNA-binding Metal-binding Zinc
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate catabolic process Inferred from mutant phenotype PubMed 11499918. Source: EcoCyc regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from direct assay PubMed 9484893. Source: EcoCyc
Cellular_component	cytoplasm Traceable author statement PubMed 11934616. Source: EcoCyc
Molecular_function	DNA binding Inferred from direct assay PubMed 11139621. Source: EcoCyc metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
mtfA	P76346	2	EBI-1116104,EBI-1126682

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 406

406

Protein mlc

PRO_0000095690

Regions

DNA binding

33 – 42

H-T-H motif By similarity

Sites

Metal binding

247

Zinc

Metal binding

257

Zinc

Metal binding

259

Zinc

Metal binding

264

Zinc

Experimental info

Mutagenesis

257

C → A or S: Strongly reduced activity. Ref.7

Mutagenesis

259

C → A or S: Strongly reduced activity. Ref.7

Sequence conflict

179

G → A in BAA06978. Ref.1

Secondary structure

406

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P50456 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 423608797FC980CB
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FASTA

406

44,316

        10         20         30         40         50         60 
MVAENQPGHI DQIKQTNAGA VYRLIDQLGP VSRIDLSRLA QLAPASITKI VREMLEAHLV 

        70         80         90        100        110        120 
QELEIKEAGN RGRPAVGLVV ETEAWHYLSL RISRGEIFLA LRDLSSKLVV EESQELALKD 

       130        140        150        160        170        180 
DLPLLDRIIS HIDQFFIRHQ KKLERLTSIA ITLPGIIDTE NGIVHRMPFY EDVKEMPLGE 

       190        200        210        220        230        240 
ALEQHTGVPV YIQHDISAWT MAEALFGASR GARDVIQVVI DHNVGAGVIT DGHLLHAGSS 

       250        260        270        280        290        300 
SLVEIGHTQV DPYGKRCYCG NHGCLETIAS VDSILELAQL RLNQSMSSML HGQPLTVDSL 

       310        320        330        340        350        360 
CQAALRGDLL AKDIITGVGA HVGRILAIMV NLFNPQKILI GSPLSKAADI LFPVISDSIR 

       370        380        390        400 
QQALPAYSQH ISVESTQFSN QGTMAGAALV KDAMYNGSLL IRLLQG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Decreasing accumulation of acetate in a rich medium by Escherichia coli on introduction of genes on a multicopy plasmid." Hosono K., Kakuda H., Ichihara S. Biosci. Biotechnol. Biochem. 59:256-261(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Purification of Mlc and analysis of its effects on the PTS expression in Escherichia coli." Kim S.-Y., Nam T.-W., Shin D., Koo B.-M., Seok Y.-J., Ryu S. J. Biol. Chem. 274:25398-25402(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Regulation of PTS gene expression by the homologous transcriptional regulators, Mlc and NagC, in Escherichia coli (or how two similar repressors can behave differently)." Plumbridge J. J. Mol. Microbiol. Biotechnol. 3:371-380(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"The crystal structure of Mlc, a global regulator of sugar metabolism in Escherichia coli." Schiefner A., Gerber K., Seitz S., Welte W., Diederichs K., Boos W. J. Biol. Chem. 280:29073-29079(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH ZINC IONS, MUTAGENESIS OF CYS-257 AND CYS-259, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D32222 Genomic DNA. Translation: BAA06978.1.
U00096 Genomic DNA. Translation: AAC74666.1.
AP009048 Genomic DNA. Translation: BAA15318.1.

PIR

D64915.

RefSeq

NP_416111.1. NC_000913.2.
YP_489857.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Z6R	X-ray	2.70	A/B/C/D	1-406	[»]
3BP8	X-ray	2.85	A/B	1-406	[»]

ProteinModelPortal

P50456.

SMR

P50456. Positions 12-406.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10219N.

IntAct

P50456. 9 interactions.

STRING

511145.b1594.

Proteomic databases

PaxDb

P50456.

PRIDE

P50456.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74666; AAC74666; b1594.
BAA15318; BAA15318; BAA15318.

GeneID

12930125.
945510.

KEGG

ecj:Y75_p1570.
eco:b1594.

PATRIC

32118490. VBIEscCol129921_1665.

Organism-specific databases

EchoBASE

EB2950.

EcoGene

EG13156. mlc.

Phylogenomic databases

eggNOG

COG1940.

HOGENOM

HOG000275182.

K15545.

OMA

PTYSQHI.

ProtClustDB

CLSK870201.

Enzyme and pathway databases

BioCyc

EcoCyc:PD01896.
ECOL316407:JW1586-MONOMER.

Gene expression databases

Genevestigator

P50456.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR000600. ROK.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00480. ROK. 1 hit.
[Graphical view]

PROSITE

PS01125. ROK. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P50456.

Entry information

Entry name

MLC_ECOLI

Accession

Primary (citable) accession number: P50456
Secondary accession number(s): P77593

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents