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P50453 (SPB9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serpin B9
Alternative name(s):
Cytoplasmic antiproteinase 3
Short name=CAP-3
Short name=CAP3
Peptidase inhibitor 9
Short name=PI-9
Gene names
Name:SERPINB9
Synonyms:PI9
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length376 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Granzyme B inhibitor.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the serpin family. Ov-serpin subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to estrogen stimulus

Inferred from expression pattern PubMed 17237823. Source: UniProtKB

immune response

Inferred from direct assay PubMed 16306080. Source: UniProtKB

mast cell mediated immunity

Inferred from expression pattern PubMed 15739160. Source: UniProtKB

negative regulation of apoptotic process

Inferred from mutant phenotype PubMed 17237823PubMed 17479112. Source: UniProtKB

negative regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from direct assay PubMed 10790428. Source: GOC

negative regulation of endopeptidase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of proteolysis

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 11463822PubMed 15739160. Source: UniProtKB

cytosol

Inferred from direct assay PubMed 11485349. Source: UniProtKB

extracellular space

Inferred from direct assay PubMed 16267761. Source: UniProtKB

extracellular vesicular exosome

Inferred from direct assay PubMed 20458337. Source: UniProt

nucleus

Inferred from direct assay PubMed 11463822PubMed 11485349. Source: UniProtKB

   Molecular_functioncysteine-type endopeptidase inhibitor activity involved in apoptotic process

Inferred from direct assay PubMed 10790428. Source: UniProtKB

protease binding

Inferred from physical interaction PubMed 16267761. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 17479112. Source: UniProtKB

serine-type endopeptidase inhibitor activity

Inferred from direct assay PubMed 11485349Ref.2. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 376376Serpin B9
PRO_0000094113

Sites

Site340 – 3412Reactive bond By similarity

Amino acid modifications

Modified residue11N-acetylmethionine Ref.11 Ref.12

Sequences

Sequence LengthMass (Da)Tools
P50453 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: ECBA80869B939753

FASTA37642,404
        10         20         30         40         50         60 
METLSNASGT FAIRLLKILC QDNPSHNVFC SPVSISSALA MVLLGAKGNT ATQMAQALSL 

        70         80         90        100        110        120 
NTEEDIHRAF QSLLTEVNKA GTQYLLRTAN RLFGEKTCQF LSTFKESCLQ FYHAELKELS 

       130        140        150        160        170        180 
FIRAAEESRK HINTWVSKKT EGKIEELLPG SSIDAETRLV LVNAIYFKGK WNEPFDETYT 

       190        200        210        220        230        240 
REMPFKINQE EQRPVQMMYQ EATFKLAHVG EVRAQLLELP YARKELSLLV LLPDDGVELS 

       250        260        270        280        290        300 
TVEKSLTFEK LTAWTKPDCM KSTEVEVLLP KFKLQEDYDM ESVLRHLGIV DAFQQGKADL 

       310        320        330        340        350        360 
SAMSAERDLC LSKFVHKSFV EVNEEGTEAA AASSCFVVAE CCMESGPRFC ADHPFLFFIR 

       370 
HNRANSILFC GRFSSP 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning, expression, and partial characterization of two novel members of the ovalbumin family of serine proteinase inhibitors."
Sprecher C.A., Morgenstern K.A., Mathewes S., Dahlen J.R., Schrader S.K., Foster D.C., Kisiel W.
J. Biol. Chem. 270:29854-29861(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[2]"A cytosolic granzyme B inhibitor related to the viral apoptotic regulator cytokine response modifier A is present in cytotoxic lymphocytes."
Sun J., Bird C.H., Sutton V., McDonald L., Coughlin P.B., Jong T.A., Trapani J.A., Bird P.I.
J. Biol. Chem. 271:27802-27809(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Bone marrow and Placenta.
[3]"Serine proteinase inhibitor 9 as a tumor antigen recognized by cytotoxic T lymphocytes of epithelial cancer patients."
Tanaka K., Harashima N., Shichijo S., Harada M., Itoh K.
Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[5]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L40378 mRNA. Translation: AAC41940.1.
U71364 mRNA. Translation: AAC50793.1.
AB060690 mRNA. Translation: BAB91078.1.
AK314842 mRNA. Translation: BAG37360.1.
BT006673 mRNA. Translation: AAP35319.1.
CR407627 mRNA. Translation: CAG28555.1.
AL133351 Genomic DNA. Translation: CAI23290.1.
CH471087 Genomic DNA. Translation: EAW55093.1.
BC002538 mRNA. Translation: AAH02538.1.
CCDSCCDS4478.1.
PIRB59273.
RefSeqNP_004146.1. NM_004155.5.
XP_005249241.1. XM_005249184.2.
UniGeneHs.104879.

3D structure databases

ProteinModelPortalP50453.
SMRP50453. Positions 1-376.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111290. 29 interactions.
IntActP50453. 25 interactions.
MINTMINT-1368440.
STRING9606.ENSP00000370074.

Protein family/group databases

MEROPSI04.014.

PTM databases

PhosphoSiteP50453.

Polymorphism databases

DMDM1709896.

2D gel databases

OGPP50453.
REPRODUCTION-2DPAGEIPI00032139.

Proteomic databases

MaxQBP50453.
PaxDbP50453.
PeptideAtlasP50453.
PRIDEP50453.

Protocols and materials databases

DNASU5272.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000380698; ENSP00000370074; ENSG00000170542.
GeneID5272.
KEGGhsa:5272.
UCSCuc003mug.4. human.

Organism-specific databases

CTD5272.
GeneCardsGC06M002887.
H-InvDBHIX0057760.
HGNCHGNC:8955. SERPINB9.
HPAHPA030067.
MIM601799. gene.
neXtProtNX_P50453.
PharmGKBPA35519.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG4826.
HOGENOMHOG000238519.
HOVERGENHBG005957.
InParanoidP50453.
KOK13963.
OMACLQFYRA.
OrthoDBEOG7327PB.
PhylomeDBP50453.
TreeFamTF352619.

Gene expression databases

ArrayExpressP50453.
BgeeP50453.
CleanExHS_SERPINB9.
GenevestigatorP50453.

Family and domain databases

InterProIPR000240. Serpin_B9/Maspin.
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
PRINTSPR00676. MASPIN.
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 1 hit.
PROSITEPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSERPINB9. human.
GeneWikiSERPINB9.
GenomeRNAi5272.
NextBio20372.
PROP50453.
SOURCESearch...

Entry information

Entry nameSPB9_HUMAN
AccessionPrimary (citable) accession number: P50453
Secondary accession number(s): B2RBW3, Q5TD03
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM