P50453 (SPB9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serpin B9

Alternative name(s):
Cytoplasmic antiproteinase 3
Short name=CAP-3
Short name=CAP3
Peptidase inhibitor 9
Short name=PI-9

Gene names

Name:	SERPINB9
Synonyms:	PI9

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Granzyme B inhibitor.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the serpin family. Ov-serpin subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Protease inhibitor Serine protease inhibitor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to estrogen stimulus Inferred from expression pattern PubMed 17237823. Source: UniProtKB immune response Inferred from direct assay PubMed 16306080. Source: UniProtKB mast cell mediated immunity Inferred from expression pattern PubMed 15739160. Source: UniProtKB negative regulation of apoptotic process Inferred from mutant phenotype PubMed 17237823PubMed 17479112. Source: UniProtKB regulation of proteolysis Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cytosol Inferred from direct assay PubMed 11485349. Source: UniProtKB extracellular space Inferred from direct assay PubMed 16267761. Source: UniProtKB nucleus Inferred from direct assay PubMed 11463822PubMed 11485349. Source: UniProtKB
Molecular_function	cysteine-type endopeptidase inhibitor activity involved in apoptotic process Inferred from direct assay PubMed 10790428. Source: UniProtKB serine-type endopeptidase inhibitor activity Inferred from direct assay PubMed 11485349Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Serpin B9		PRO_0000094113
Sites
	Site	340 – 341	2	Reactive bond By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50453 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: ECBA80869B939753	FASTA	376	42,404
	10 20 30 40 50 60 METLSNASGT FAIRLLKILC QDNPSHNVFC SPVSISSALA MVLLGAKGNT ATQMAQALSL 70 80 90 100 110 120 NTEEDIHRAF QSLLTEVNKA GTQYLLRTAN RLFGEKTCQF LSTFKESCLQ FYHAELKELS 130 140 150 160 170 180 FIRAAEESRK HINTWVSKKT EGKIEELLPG SSIDAETRLV LVNAIYFKGK WNEPFDETYT 190 200 210 220 230 240 REMPFKINQE EQRPVQMMYQ EATFKLAHVG EVRAQLLELP YARKELSLLV LLPDDGVELS 250 260 270 280 290 300 TVEKSLTFEK LTAWTKPDCM KSTEVEVLLP KFKLQEDYDM ESVLRHLGIV DAFQQGKADL 310 320 330 340 350 360 SAMSAERDLC LSKFVHKSFV EVNEEGTEAA AASSCFVVAE CCMESGPRFC ADHPFLFFIR 370 HNRANSILFC GRFSSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, expression, and partial characterization of two novel members of the ovalbumin family of serine proteinase inhibitors." Sprecher C.A., Morgenstern K.A., Mathewes S., Dahlen J.R., Schrader S.K., Foster D.C., Kisiel W. J. Biol. Chem. 270:29854-29861(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"A cytosolic granzyme B inhibitor related to the viral apoptotic regulator cytokine response modifier A is present in cytotoxic lymphocytes." Sun J., Bird C.H., Sutton V., McDonald L., Coughlin P.B., Jong T.A., Trapani J.A., Bird P.I. J. Biol. Chem. 271:27802-27809(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Bone marrow and Placenta.
[3]	"Serine proteinase inhibitor 9 as a tumor antigen recognized by cytotoxic T lymphocytes of epithelial cancer patients." Tanaka K., Harashima N., Shichijo S., Harada M., Itoh K. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[5]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L40378 mRNA. Translation: AAC41940.1. U71364 mRNA. Translation: AAC50793.1. AB060690 mRNA. Translation: BAB91078.1. AK314842 mRNA. Translation: BAG37360.1. BT006673 mRNA. Translation: AAP35319.1. CR407627 mRNA. Translation: CAG28555.1. AL133351 Genomic DNA. Translation: CAI23290.1. CH471087 Genomic DNA. Translation: EAW55093.1. BC002538 mRNA. Translation: AAH02538.1.
IPI	IPI00032139.
PIR	B59273.
RefSeq	NP_004146.1. NM_004155.5.
UniGene	Hs.104879.
3D structure databases
ProteinModelPortal	P50453.
ModBase	Search...
Protein-protein interaction databases
IntAct	P50453. 25 interactions.
MINT	MINT-1368440.
STRING	9606.ENSP00000370074.
Protein family/group databases
MEROPS	I04.014.
PTM databases
PhosphoSite	P50453.
Polymorphism databases
DMDM	1709896.
2D gel databases
OGP	P50453.
REPRODUCTION-2DPAGE	IPI00032139.
Proteomic databases
PaxDb	P50453.
PeptideAtlas	P50453.
PRIDE	P50453.
Protocols and materials databases
DNASU	5272.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000380698; ENSP00000370074; ENSG00000170542.
GeneID	5272.
KEGG	hsa:5272.
UCSC	uc003mug.3. human.
Organism-specific databases
CTD	5272.
GeneCards	GC06M002887.
H-InvDB	HIX0057760.
HGNC	HGNC:8955. SERPINB9.
MIM	601799. gene.
neXtProt	NX_P50453.
PharmGKB	PA35519.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG4826.
HOGENOM	HOG000238519.
HOVERGEN	HBG005957.
InParanoid	P50453.
KO	K13963.
OMA	QMMFQEA.
OrthoDB	EOG412M5X.
PhylomeDB	P50453.
Gene expression databases
ArrayExpress	P50453.
Bgee	P50453.
CleanEx	HS_SERPINB9.
Genevestigator	P50453.
GermOnline	ENSG00000170542. Homo sapiens.
Family and domain databases
InterPro	IPR023795. Protease_inhib_I4_serpin_CS. IPR000240. Serpin_B9/Maspin. IPR023796. Serpin_dom. IPR000215. Serpin_fam. [Graphical view]
PANTHER	PTHR11461. PTHR11461. 1 hit.
Pfam	PF00079. Serpin. 1 hit. [Graphical view]
PRINTS	PR00676. MASPIN.
SMART	SM00093. SERPIN. 1 hit. [Graphical view]
SUPFAM	SSF56574. Prot_inh_serpin. 1 hit.
PROSITE	PS00284. SERPIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SERPINB9. human.
GenomeRNAi	5272.
NextBio	20372.
SOURCE	Search...

Entry information

Entry name

SPB9_HUMAN

Accession

Primary (citable) accession number: P50453
Secondary accession number(s): B2RBW3, Q5TD03

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 126 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.