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P50452 (SPB8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serpin B8
Alternative name(s):
Cytoplasmic antiproteinase 2
Short name=CAP-2
Short name=CAP2
Peptidase inhibitor 8
Short name=PI-8
Gene names
Name:SERPINB8
Synonyms:PI8
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the serpin family. Ov-serpin subfamily.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P50452-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P50452-2)

The sequence of this isoform differs from the canonical sequence as follows:
     241-374: VEKALTYEKF...ILFCGRFSSP → KE
Note: No experimental confirmation available.
Isoform 3 (identifier: P50452-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-182: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 374374Serpin B8
PRO_0000094111

Sites

Site339 – 3402Reactive bond By similarity

Natural variations

Alternative sequence1 – 182182Missing in isoform 3.
VSP_055135
Alternative sequence241 – 374134VEKAL…RFSSP → KE in isoform 2.
VSP_043097
Natural variant681R → Q.
Corresponds to variant rs1944270 [ dbSNP | Ensembl ].
VAR_047110
Natural variant1581K → N.
Corresponds to variant rs1648493 [ dbSNP | Ensembl ].
VAR_047111
Natural variant3041T → A. Ref.3
Corresponds to variant rs3169983 [ dbSNP | Ensembl ].
VAR_051947
Natural variant3591H → R. Ref.1
Corresponds to variant rs3826616 [ dbSNP | Ensembl ].
VAR_051948

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 4, 2008. Version 2.
Checksum: 7235A033519AC612

FASTA37442,767
        10         20         30         40         50         60 
MDDLCEANGT FAISLFKILG EEDNSRNVFF SPMSISSALA MVFMGAKGST AAQMSQALCL 

        70         80         90        100        110        120 
YKDGDIHRGF QSLLSEVNRT GTQYLLRTAN RLFGEKTCDF LPDFKEYCQK FYQAELEELS 

       130        140        150        160        170        180 
FAEDTEECRK HINDWVAEKT EGKISEVLDA GTVDPLTKLV LVNAIYFKGK WNEQFDRKYT 

       190        200        210        220        230        240 
RGMLFKTNEE KKTVQMMFKE AKFKMGYADE VHTQVLELPY VEEELSMVIL LPDDNTDLAV 

       250        260        270        280        290        300 
VEKALTYEKF KAWTNSEKLT KSKVQVFLPR LKLEESYDLE PFLRRLGMID AFDEAKADFS 

       310        320        330        340        350        360 
GMSTEKNVPL SKVAHKCFVE VNEEGTEAAA ATAVVRNSRC SRMEPRFCAD HPFLFFIRHH 

       370 
KTNCILFCGR FSSP 

« Hide

Isoform 2 [UniParc].

Checksum: B7CF7D13B3129681
Show »

FASTA24227,673
Isoform 3 [UniParc].

Checksum: EBDED15A48B73B5D
Show »

FASTA19222,134

References

« Hide 'large scale' references
[1]"Molecular cloning, expression, and partial characterization of two novel members of the ovalbumin family of serine proteinase inhibitors."
Sprecher C.A., Morgenstern K.A., Mathewes S., Dahlen J.R., Schrader S.K., Foster D.C., Kisiel W.
J. Biol. Chem. 270:29854-29861(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ARG-359.
Tissue: Placenta.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Placenta.
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-304.
Tissue: Esophagus.
[4]"DNA sequence and analysis of human chromosome 18."
Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. expand/collapse author list , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.
Nature 437:551-555(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Skin.
[6]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L40377 mRNA. Translation: AAC41939.1.
AK300391 mRNA. Translation: BAG62124.1.
BX571754 mRNA. Translation: CAE11879.1.
AC009802 Genomic DNA. No translation available.
BC034528 mRNA. Translation: AAH34528.1.
CCDSCCDS11991.1. [P50452-1]
CCDS42442.1. [P50452-2]
PIRA59273.
RefSeqNP_001027018.1. NM_001031848.1. [P50452-2]
NP_001263419.1. NM_001276490.1.
NP_002631.3. NM_002640.3. [P50452-1]
NP_942130.1. NM_198833.1. [P50452-1]
UniGeneHs.368077.

3D structure databases

ProteinModelPortalP50452.
SMRP50452. Positions 1-374.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111289. 6 interactions.
STRING9606.ENSP00000331368.

Protein family/group databases

MEROPSI04.013.

PTM databases

PhosphoSiteP50452.

Polymorphism databases

DMDM212276474.

Proteomic databases

MaxQBP50452.
PaxDbP50452.
PRIDEP50452.

Protocols and materials databases

DNASU5271.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000353706; ENSP00000331368; ENSG00000166401. [P50452-1]
ENST00000397985; ENSP00000381072; ENSG00000166401. [P50452-1]
ENST00000397988; ENSP00000381075; ENSG00000166401. [P50452-2]
ENST00000542677; ENSP00000438328; ENSG00000166401.
GeneID5271.
KEGGhsa:5271.
UCSCuc002ljt.3. human. [P50452-2]
uc002lju.3. human. [P50452-1]

Organism-specific databases

CTD5271.
GeneCardsGC18P061637.
HGNCHGNC:8952. SERPINB8.
HPACAB034300.
MIM601697. gene.
neXtProtNX_P50452.
PharmGKBPA35517.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG4826.
HOGENOMHOG000238519.
HOVERGENHBG005957.
InParanoidP50452.
KOK13965.
OMAIRHHKTN.
OrthoDBEOG7327PB.
PhylomeDBP50452.
TreeFamTF352619.

Enzyme and pathway databases

ReactomeREACT_604. Hemostasis.

Gene expression databases

ArrayExpressP50452.
BgeeP50452.
CleanExHS_SERPINB8.
GenevestigatorP50452.

Family and domain databases

InterProIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 1 hit.
PROSITEPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSERPINB8.
GenomeRNAi5271.
NextBio20364.
PROP50452.
SOURCESearch...

Entry information

Entry nameSPB8_HUMAN
AccessionPrimary (citable) accession number: P50452
Secondary accession number(s): B4DTW2, Q7Z2V6, Q8N178
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 4, 2008
Last modified: July 9, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM