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Protein

Glycine amidinotransferase, mitochondrial

Gene

GATM

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the biosynthesis of guanidinoacetate, the immediate precursor of creatine. Creatine plays a vital role in energy metabolism in muscle tissues. May play a role in embryonic and central nervous system development (By similarity).By similarity

Catalytic activityi

L-arginine + glycine = L-ornithine + guanidinoacetate.

Pathwayi: creatine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes creatine from L-arginine and glycine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glycine amidinotransferase, mitochondrial (GATM)
  2. no protein annotated in this organism
This subpathway is part of the pathway creatine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes creatine from L-arginine and glycine, the pathway creatine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei254 – 2541By similarity
Active sitei303 – 3031By similarity
Active sitei407 – 4071Amidino-cysteine intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiR-SSC-71288. Creatine metabolism.
UniPathwayiUPA00104; UER00579.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine amidinotransferase, mitochondrial (EC:2.1.4.1)
Alternative name(s):
L-arginine:glycine amidinotransferase
Transamidinase
Gene namesi
Name:GATM
Synonyms:AGAT
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3737Mitochondrion1 PublicationAdd
BLAST
Chaini38 – 423386Glycine amidinotransferase, mitochondrialPRO_0000215474Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei46 – 461PhosphoserineBy similarity
Modified residuei49 – 491PhosphoserineBy similarity
Modified residuei385 – 3851N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP50441.

Expressioni

Tissue specificityi

Kidney. Expressed biallelically in placenta.1 Publication

Developmental stagei

Expressed in placenta on days 75 and 90 of gestation.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000005032.

Structurei

3D structure databases

ProteinModelPortaliP50441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidinotransferase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IFBR. Eukaryota.
ENOG410Y45M. LUCA.
GeneTreeiENSGT00390000011613.
HOGENOMiHOG000231593.
HOVERGENiHBG002492.
InParanoidiP50441.
KOiK00613.
OMAiRPCHQID.
OrthoDBiEOG712TW4.
TreeFamiTF300256.

Family and domain databases

InterProiIPR033195. AmidinoTrfase.
[Graphical view]
PANTHERiPTHR10488. PTHR10488. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50441-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRVRCLRGG SRGAEALHYI GSRLGRTVTG WVQRTFQSTQ AATASSGNSC
60 70 80 90 100
AADDKATDPL PKDCPVSSYN EWDPLEEVIV GRAENACVPP FTVEVKANTY
110 120 130 140 150
EKYWPFYQKY GGHYFPKDHL KKAVAEIEEM CNILKMEGVT VRRPDPIDWS
160 170 180 190 200
VKYKTPDFES TGLYGAMPRD ILIVVGNEII EAPMAWRARF FEYRAYRSII
210 220 230 240 250
KDYFRRGAKW TTAPKPTMAD ELYDQDYPIY SVEDRHKLAA QGKFVTTEFE
260 270 280 290 300
PCFDAADFIR AGRDIFAQRS QVTNYMGIEW MRKHLAPDYR VHIISFKDPN
310 320 330 340 350
PMHIDATFNI IGPGLVLSNP DRPCHQIDLF KKAGWTIVTP PIPVIPDDHP
360 370 380 390 400
LWMSSKWLSM NVLMLDEKRV MVDANEVPIQ KMFEKLGIST IKISIRNANS
410 420
LGGGFHCWTC DVRRRGTLQS YFD
Length:423
Mass (Da):48,360
Last modified:October 5, 2010 - v2
Checksum:iF83FC21A1B14B018
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF612462 mRNA. Translation: ABS83814.1.
AY625268 mRNA. Translation: AAT39894.1.
PIRiS40296.
RefSeqiNP_001121914.1. NM_001128442.1.
UniGeneiSsc.57420.

Genome annotation databases

EnsembliENSSSCT00000005158; ENSSSCP00000005032; ENSSSCG00000004672.
GeneIDi100126844.
KEGGissc:100126844.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF612462 mRNA. Translation: ABS83814.1.
AY625268 mRNA. Translation: AAT39894.1.
PIRiS40296.
RefSeqiNP_001121914.1. NM_001128442.1.
UniGeneiSsc.57420.

3D structure databases

ProteinModelPortaliP50441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000005032.

Proteomic databases

PaxDbiP50441.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000005158; ENSSSCP00000005032; ENSSSCG00000004672.
GeneIDi100126844.
KEGGissc:100126844.

Organism-specific databases

CTDi2628.

Phylogenomic databases

eggNOGiENOG410IFBR. Eukaryota.
ENOG410Y45M. LUCA.
GeneTreeiENSGT00390000011613.
HOGENOMiHOG000231593.
HOVERGENiHBG002492.
InParanoidiP50441.
KOiK00613.
OMAiRPCHQID.
OrthoDBiEOG712TW4.
TreeFamiTF300256.

Enzyme and pathway databases

UniPathwayiUPA00104; UER00579.
ReactomeiR-SSC-71288. Creatine metabolism.

Family and domain databases

InterProiIPR033195. AmidinoTrfase.
[Graphical view]
PANTHERiPTHR10488. PTHR10488. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Imprinting analyses of the porcine GATM and PEG10 genes in placentas on days 75 and 90 of gestation."
    Zhou Q.Y., Huang J.N., Xiong Y.Z., Zhao S.H.
    Genes Genet. Syst. 82:265-269(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "The amino acid sequences of human and pig L-arginine:glycine amidinotransferase."
    Humm A., Huber R., Mann K.
    FEBS Lett. 339:101-107(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 38-423.
    Tissue: Kidney.
  3. "The comparative cDNA sequences of some mammalian kidney L-arginine:glycine amidinotransferase genes and their evolutionary significance."
    Zink R.M., Westberg M.C., Van Pilsum J.F.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 123-362.

Entry informationi

Entry nameiGATM_PIG
AccessioniPrimary (citable) accession number: P50441
Secondary accession number(s): B3F4S6, Q6IU00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 5, 2010
Last modified: May 11, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.