Reviewed, UniProtKB/Swiss-Prot P50430 (ARSB_RAT)

Names and origin

Protein names

Recommended name:
    Arylsulfatase B
      Short name=ASB
    EC=3.1.6.12
Alternative name(s):
    N-acetylgalactosamine-4-sulfatase
      Short name=G4S

Gene names

Name:

Arsb

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of the 4-sulfate groups of the N-acetyl-D-galactosamine 4-sulfate units of chondroitin sulfate and dermatan sulfate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Lysosome.
Involvement in disease	Defects in Arsb are the cause of mucopolysaccharidosis type VI (MPS-VI).
Sequence similarities	Belongs to the sulfatase family.

Ontologies

Keywords
Cellular component	Lysosome
Disease	Mucopolysaccharidosis
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	autophagy Inferred from direct assay. Source: RGD central nervous system development Inferred from direct assay. Source: RGD response to estrogen stimulus Inferred from direct assay. Source: RGD response to methylmercury Inferred from direct assay. Source: RGD response to nutrient Inferred from direct assay. Source: RGD response to pH Inferred from direct assay. Source: RGD
Cellular component	Golgi apparatus Inferred from direct assay. Source: RGD lysosome Inferred from direct assay. Source: RGD mitochondrion Inferred from direct assay. Source: RGD rough endoplasmic reticulum Inferred from direct assay. Source: RGD
Molecular function	N-acetylgalactosamine-4-sulfatase activity Inferred from electronic annotation. Source: EC arylsulfatase activity Ref.2 Traceable author statement. Source: RGD calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 528	528	Arylsulfatase B		PRO_0000192682
Sites
	Active site	142	1	By similarity
	Metal binding	48	1	Calcium By similarity
	Metal binding	49	1	Calcium By similarity
	Metal binding	86	1	Calcium; via 3-oxoalanine By similarity
	Metal binding	295	1	Calcium By similarity
	Metal binding	296	1	Calcium By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	237	1	Substrate By similarity
	Binding site	313	1	Substrate By similarity
Amino acid modifications
	Modified residue	86	1	3-oxoalanine (Cys) By similarity
	Glycosylation	183	1	N-linked (GlcNAc...) Potential
	Glycosylation	274	1	N-linked (GlcNAc...) Potential
	Glycosylation	286	1	N-linked (GlcNAc...) Potential
	Glycosylation	410	1	N-linked (GlcNAc...) Potential
	Glycosylation	421	1	N-linked (GlcNAc...) Potential
	Glycosylation	453	1	N-linked (GlcNAc...) Potential
	Disulfide bond	112 ↔ 516		By similarity
	Disulfide bond	116 ↔ 150		By similarity
	Disulfide bond	176 ↔ 187		By similarity
	Disulfide bond	400 ↔ 442		By similarity
Experimental info
	Sequence conflict	56 – 57	2	GF → IR in AABR03012149. Ref.1
	Sequence conflict	133 – 134	2	YA → SS in AABR03012149. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P50430-1 [UniParc]. Last modified June 12, 2007. Version 2. Checksum: 1F02425E858E5A00	FASTA	528	58,959
	10 20 30 40 50 60 MGELSGCTGG SRAGGPGPRL PLLLLLLLWP ARASDAAPPP HVVFVLADDL GWNDLGFHGS 70 80 90 100 110 120 VIRTPHLDAL AAGGVVLDNY YVQPLCTPSR SQLLTGRYQI HMGLQHYLIM TCQPNCVPLD 130 140 150 160 170 180 EKLLPQLLKD AGYATHMVGK WHLGMYRKEC LPTRRGFDTY FGYLLGSEDY YTHEACAPIE 190 200 210 220 230 240 CLNGTRCALD LRDGEEPAKE YTDIYSTNIF TKRATTLIAN HPPEKPLFLY LAFQSVHDPL 250 260 270 280 290 300 QVPEEYMEPY DFIQDKHRRI YAGMVSLLDE AVGNVTKALK SRGLWNNTVL IFSTDNGGQT 310 320 330 340 350 360 RSGGNNWPLR GRKGTLWEGG IRGAGFVASP LLKQKGVKSR ELMHITDWLP TLVNLAGGST 370 380 390 400 410 420 HGTKPLDGFD VWETISEGSP SPRVELLLNI DPDFFDGLPC PGKNTTPEKN DSFPLEHSAF 430 440 450 460 470 480 NTSIHAGIRY KNWKLLTGYP GCGYWFPPPS QSNISEVPSV DSPTKTLWLF DINRDPEERH 490 500 510 520 DVSREHPHIV QNLLSRLQYY HEHSVPSYFP PLDPRCDPKG TGVWSPWM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed: 15057822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[2]	"Mucopolysaccharidosis type VI in rats: isolation of cDNAs encoding arylsulfatase B, chromosomal localization of the gene, and identification of the mutation." Kunieda T. Genomics 29:582-587(1995) [PubMed: 8575749] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-55. Strain: Sprague-Dawley. Tissue: Liver.
[3]	"Sulfatases and sulfatase modifying factors: an exclusive and promiscuous relationship." Sardiello M., Annunziata I., Roma G., Ballabio A. Hum. Mol. Genet. 14:3203-3217(2005) [PubMed: 16174644] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AABR03012149 Genomic DNA. No translation available. AABR03015281 Genomic DNA. No translation available. AABR03016930 Genomic DNA. No translation available. AABR03021723 Genomic DNA. No translation available. D49434 mRNA. Translation: BAA08412.1. BN000736 mRNA. Translation: CAI84982.1.
IPI	IPI00198405.
PIR	I54210.
RefSeq	NP_254278.1.
UniGene	Rn.94004
3D structure databases
HSSP	HSSP built from PDB template 1FSU based on UniProtKB P15848.
ModBase	Search...
Protein-protein interaction databases
STRING	P50430.
Proteomic databases
PRIDE	P50430.
Genome annotation databases
Ensembl	ENSRNOT00000014860; ENSRNOP00000014860; ENSRNOG00000011150; Rattus norvegicus. [Genome view]
GeneID	25227.
KEGG	rno:25227.
NMPDR	fig|10116.3.peg.15356.
UCSC	NM_033443. rat.
Organism-specific databases
CTD	25227.
RGD	2158. Arsb.
Phylogenomic databases
HOVERGEN	P50430.
OMA	GYPGCGY.
Enzyme and pathway databases
BRENDA	3.1.6.12. 248.
Gene expression databases
ArrayExpress	P50430.
Genevestigator	P50430.
GermOnline	ENSRNOG00000011150. Rattus norvegicus.
Family and domain databases
InterPro	IPR000917. Sulfatase. [Graphical view]
Pfam	PF00884. Sulfatase. 1 hit. [Graphical view]
PROSITE	PS00523. SULFATASE_1. 1 hit. PS00149. SULFATASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	605779.

Entry information

Entry name

ARSB_RAT

Accession

Primary (citable) accession number: P50430
Secondary accession number(s): Q32KK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)