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Protein

Arylsulfatase B

Gene

Arsb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Removes sulfate groups from chondroitin-4-sulfate (C4S) and regulates its degradation (PubMed:24311516). In the central nervous system, is a regulator of neurite outgrowth and neuronal plasticity, acting through the control of sulfate glycosaminoglycans and neurocan levels (PubMed:24311516). Involved in the regulation of cell adhesion, cell migration and invasion in colonic epithelium (By similarity).By similarity1 Publication

Catalytic activityi

Hydrolysis of the 4-sulfate groups of the N-acetyl-D-galactosamine 4-sulfate units of chondroitin sulfate and dermatan sulfate.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Enzyme regulationi

Inhibited by ethanol.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48CalciumBy similarity1
Metal bindingi49CalciumBy similarity1
Active sitei86NucleophileBy similarity1
Metal bindingi86Calcium; via 3-oxoalanineBy similarity1
Binding sitei140SubstrateBy similarity1
Active sitei142By similarity1
Binding sitei237SubstrateBy similarity1
Metal bindingi295CalciumBy similarity1
Metal bindingi296CalciumBy similarity1
Binding sitei313SubstrateBy similarity1

GO - Molecular functioni

  • arylsulfatase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • N-acetylgalactosamine-4-sulfatase activity Source: UniProtKB
  • sulfuric ester hydrolase activity Source: RGD

GO - Biological processi

  • autophagy Source: RGD
  • central nervous system development Source: RGD
  • colon epithelial cell migration Source: UniProtKB
  • positive regulation of neuron projection development Source: UniProtKB
  • regulation of epithelial cell migration Source: UniProtKB
  • response to estrogen Source: RGD
  • response to methylmercury Source: RGD
  • response to nutrient Source: RGD
  • response to pH Source: RGD

Keywordsi

Molecular functionHydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-1660662 Glycosphingolipid metabolism
R-RNO-1663150 The activation of arylsulfatases
R-RNO-2024101 CS/DS degradation
R-RNO-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Arylsulfatase B (EC:3.1.6.12)
Short name:
ASB
Alternative name(s):
N-acetylgalactosamine-4-sulfatase
Short name:
G4S
Gene namesi
Name:Arsb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi2158 Arsb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Involvement in diseasei

Defects in Arsb are the cause of mucopolysaccharidosis type VI (MPS-VI) (PubMed:8575749).1 Publication

Keywords - Diseasei

Mucopolysaccharidosis

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001926821 – 528Arylsulfatase BAdd BLAST528

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei863-oxoalanine (Cys)By similarity1
Disulfide bondi112 ↔ 516By similarity
Disulfide bondi116 ↔ 150By similarity
Disulfide bondi176 ↔ 187By similarity
Glycosylationi183N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi274N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi286N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi400 ↔ 442By similarity
Glycosylationi410N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi421N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi453N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP50430
PRIDEiP50430

PTM databases

PhosphoSitePlusiP50430

Expressioni

Gene expression databases

BgeeiENSRNOG00000011150
ExpressionAtlasiP50430 baseline and differential
GenevisibleiP50430 RN

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi247268, 1 interactor
IntActiP50430, 1 interactor
STRINGi10116.ENSRNOP00000014860

Structurei

3D structure databases

ProteinModelPortaliP50430
SMRiP50430
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Phylogenomic databases

eggNOGiKOG3867 Eukaryota
COG3119 LUCA
GeneTreeiENSGT00760000119062
HOGENOMiHOG000135354
HOVERGENiHBG004282
InParanoidiP50430
KOiK01135
OMAiKWTLWEG
OrthoDBiEOG091G06C3
PhylomeDBiP50430
TreeFamiTF314186

Family and domain databases

Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR017850 Alkaline_phosphatase_core_sf
IPR024607 Sulfatase_CS
IPR000917 Sulfatase_N
PfamiView protein in Pfam
PF00884 Sulfatase, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00523 SULFATASE_1, 1 hit
PS00149 SULFATASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P50430-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGELSGCTGG SRAGGPGPRL PLLLLLLLWP ARASDAAPPP HVVFVLADDL
60 70 80 90 100
GWNDLGFHGS VIRTPHLDAL AAGGVVLDNY YVQPLCTPSR SQLLTGRYQI
110 120 130 140 150
HMGLQHYLIM TCQPNCVPLD EKLLPQLLKD AGYATHMVGK WHLGMYRKEC
160 170 180 190 200
LPTRRGFDTY FGYLLGSEDY YTHEACAPIE CLNGTRCALD LRDGEEPAKE
210 220 230 240 250
YTDIYSTNIF TKRATTLIAN HPPEKPLFLY LAFQSVHDPL QVPEEYMEPY
260 270 280 290 300
DFIQDKHRRI YAGMVSLLDE AVGNVTKALK SRGLWNNTVL IFSTDNGGQT
310 320 330 340 350
RSGGNNWPLR GRKGTLWEGG IRGAGFVASP LLKQKGVKSR ELMHITDWLP
360 370 380 390 400
TLVNLAGGST HGTKPLDGFD VWETISEGSP SPRVELLLNI DPDFFDGLPC
410 420 430 440 450
PGKNTTPEKN DSFPLEHSAF NTSIHAGIRY KNWKLLTGYP GCGYWFPPPS
460 470 480 490 500
QSNISEVPSV DSPTKTLWLF DINRDPEERH DVSREHPHIV QNLLSRLQYY
510 520
HEHSVPSYFP PLDPRCDPKG TGVWSPWM
Length:528
Mass (Da):58,959
Last modified:June 12, 2007 - v2
Checksum:i1F02425E858E5A00
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56 – 57GF → IR in AABR03012149 (PubMed:15057822).Curated2
Sequence conflicti133 – 134YA → SS in AABR03012149 (PubMed:15057822).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03012149 Genomic DNA No translation available.
AABR03015281 Genomic DNA No translation available.
AABR03016930 Genomic DNA No translation available.
AABR03021723 Genomic DNA No translation available.
D49434 mRNA Translation: BAA08412.1
BN000736 mRNA Translation: CAI84982.1
PIRiI54210
RefSeqiNP_254278.1, NM_033443.1
UniGeneiRn.94004

Genome annotation databases

EnsembliENSRNOT00000014860; ENSRNOP00000014860; ENSRNOG00000011150
GeneIDi25227
KEGGirno:25227
UCSCiRGD:2158 rat

Similar proteinsi

Entry informationi

Entry nameiARSB_RAT
AccessioniPrimary (citable) accession number: P50430
Secondary accession number(s): Q32KK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 12, 2007
Last modified: May 23, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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