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Protein

Arylsulfatase B

Gene

Arsb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes sulfate groups from chondroitin-4-sulfate (C4S) and regulates its degradation (By similarity). Involved in the regulation of cell adhesion, cell migration and invasion in colonic epithelium (By similarity). In the central nervous system, is a regulator of neurite outgrowth and neuronal plasticity, acting through the control of sulfate glycosaminoglycans and neurocan levels (By similarity).By similarity

Catalytic activityi

Hydrolysis of the 4-sulfate groups of the N-acetyl-D-galactosamine 4-sulfate units of chondroitin sulfate and dermatan sulfate.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Enzyme regulationi

Inhibited by ethanol (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54CalciumBy similarity1
Metal bindingi55CalciumBy similarity1
Active sitei92NucleophileBy similarity1
Metal bindingi92Calcium; via 3-oxoalanineBy similarity1
Binding sitei146SubstrateBy similarity1
Active sitei148By similarity1
Binding sitei243SubstrateBy similarity1
Metal bindingi301CalciumBy similarity1
Metal bindingi302CalciumBy similarity1
Binding sitei319SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

SABIO-RKP50429

Names & Taxonomyi

Protein namesi
Recommended name:
Arylsulfatase B (EC:3.1.6.12)
Short name:
ASB
Alternative name(s):
N-acetylgalactosamine-4-sulfatase
Short name:
G4S
Gene namesi
Name:Arsb
Synonyms:As1, As1-s
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88075 Arsb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 41Sequence analysisAdd BLAST41
ChainiPRO_000003342342 – 534Arylsulfatase BAdd BLAST493

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei923-oxoalanine (Cys)By similarity1
Disulfide bondi118 ↔ 522By similarity
Disulfide bondi122 ↔ 156By similarity
Disulfide bondi182 ↔ 193By similarity
Glycosylationi189N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi280N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi292N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi367N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi406 ↔ 448By similarity
Glycosylationi427N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi459N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP50429
MaxQBiP50429
PaxDbiP50429
PeptideAtlasiP50429
PRIDEiP50429

PTM databases

PhosphoSitePlusiP50429

Expressioni

Gene expression databases

CleanExiMM_ARSB

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000088964

Structurei

3D structure databases

ProteinModelPortaliP50429
SMRiP50429
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3867 Eukaryota
COG3119 LUCA
HOGENOMiHOG000135354
HOVERGENiHBG004282
InParanoidiP50429
KOiK01135
PhylomeDBiP50429
TreeFamiTF314186

Family and domain databases

Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR017850 Alkaline_phosphatase_core_sf
IPR024607 Sulfatase_CS
IPR000917 Sulfatase_N
PfamiView protein in Pfam
PF00884 Sulfatase, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00523 SULFATASE_1, 1 hit
PS00149 SULFATASE_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P50429-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKLSPCTGR SRPGGPGPQL PLLLLLLQLL LLLLSPARAS GATQPPHVVF
60 70 80 90 100
VLADDLGWND LGFHGSVIRT PHLDALAAGG VVLDNYYVQP LCTPSRSQLL
110 120 130 140 150
TGRYQIHLGL QHYLIMTCQP SCVPLDEKLL PQLLKEAGYA THMVGKWHLG
160 170 180 190 200
MYRKECLPTR RGFDTYFGYL LGSEDYYTHE ACAPIESLNG TRCALDLRDG
210 220 230 240 250
EEPAKEYNNI YSTNIFTKRA TTVIANHPPE KPLFLYLAFQ SVHDPLQVPE
260 270 280 290 300
EYMEPYGFIQ DKHRRIYAGM VSLMDEAVGN VTKALKSHGL WNNTVFIFST
310 320 330 340 350
DNGGQTRSGG NNWPLRGRKG TLWEGGIRGT GFVASPLLKQ KGVKSRELMH
360 370 380 390 400
ITDWLPTLVD LAGGSTNGTK PLDGFNMWKT ISEGHPSPRV ELLHNIDQDF
410 420 430 440 450
FDGLPCPGKN MTPAKDDSFP LEHSAFNTSI HAGIRYKNWK LLTGHPGCGY
460 470 480 490 500
WFPPPSQSNV SEIPPVGPPT KTLWLFDINQ DPEERHDVSR EHPHIVQNLL
510 520 530
SRLQYYHEHS VPSHFPPLDP RCDPKSTGVW SPWM
Length:534
Mass (Da):59,647
Last modified:January 9, 2007 - v3
Checksum:i78DAB2D65C71E97D
GO
Isoform 2 (identifier: P50429-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     383-431: EGHPSPRVEL...EHSAFNTSIH → PVTGDHWHAE...PDCGRARWFL
     432-534: Missing.

Show »
Length:431
Mass (Da):47,934
Checksum:iE3C8BBE3E0B7C6D5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60D → A in AAA37261 (PubMed:1572648).Curated1
Sequence conflicti352T → S in BAE34455 (PubMed:16141072).Curated1
Sequence conflicti352T → S in CAA63067 (PubMed:8710849).Curated1
Sequence conflicti467G → D in CAI84992 (PubMed:19468303).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007881383 – 431EGHPS…NTSIH → PVTGDHWHAEGELGCSFRTA SAAEEEPTYKLREKKRRKSP DCGRARWFL in isoform 2. 1 PublicationAdd BLAST49
Alternative sequenceiVSP_022249432 – 534Missing in isoform 2. 1 PublicationAdd BLAST103

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK083309 mRNA Translation: BAC38859.1
AK154098 mRNA Translation: BAE32375.1
AK158312 mRNA Translation: BAE34455.1
AC131739 Genomic DNA No translation available.
AC136976 Genomic DNA No translation available.
M82877 mRNA Translation: AAA37261.1
X92096 mRNA Translation: CAA63067.1
BN000746 mRNA Translation: CAI84992.1
CCDSiCCDS36749.1 [P50429-1]
RefSeqiNP_033842.3, NM_009712.3
UniGeneiMm.300178
Mm.472255

Genome annotation databases

GeneIDi11881
KEGGimmu:11881
UCSCiuc007rlo.1 mouse [P50429-1]
uc011zcv.1 mouse [P50429-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiARSB_MOUSE
AccessioniPrimary (citable) accession number: P50429
Secondary accession number(s): Q32KJ1
, Q3TYV7, Q3U4Q6, Q8C404
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 9, 2007
Last modified: March 28, 2018
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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