P50429 (ARSB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arylsulfatase B
Short name=ASB
EC=3.1.6.12

Alternative name(s):
N-acetylgalactosamine-4-sulfatase
Short name=G4S

Gene names

Name:	Arsb
Synonyms:	As1, As1-s

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of the 4-sulfate groups of the N-acetyl-D-galactosamine 4-sulfate units of chondroitin sulfate and dermatan sulfate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Lysosome.
Post-translational modification	The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity By similarity.
Sequence similarities	Belongs to the sulfatase family.

Ontologies

Keywords
Cellular component	Lysosome
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagy Inferred from electronic annotation. Source: Compara central nervous system development Inferred from electronic annotation. Source: Compara response to estrogen stimulus Inferred from electronic annotation. Source: Compara response to methylmercury Inferred from electronic annotation. Source: Compara response to nutrient Inferred from electronic annotation. Source: Compara response to pH Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from electronic annotation. Source: Compara rough endoplasmic reticulum Inferred from electronic annotation. Source: Compara
Molecular_function	N-acetylgalactosamine-4-sulfatase activity Inferred from electronic annotation. Source: EC arylsulfatase activity Inferred from direct assay PubMed 7470017. Source: MGI metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P50429-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P50429-2)

The sequence of this isoform differs from the canonical sequence as follows:
     383-431: EGHPSPRVEL...EHSAFNTSIH → PVTGDHWHAE...PDCGRARWFL
     432-534: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 41	41	Potential
	Chain	42 – 534	493	Arylsulfatase B		PRO_0000033423
Sites
	Active site	148	1	By similarity
	Metal binding	54	1	Calcium By similarity
	Metal binding	55	1	Calcium By similarity
	Metal binding	92	1	Calcium; via 3-oxoalanine By similarity
	Metal binding	301	1	Calcium By similarity
	Metal binding	302	1	Calcium By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	319	1	Substrate By similarity
Amino acid modifications
	Modified residue	92	1	3-oxoalanine (Cys) By similarity
	Glycosylation	189	1	N-linked (GlcNAc...) Potential
	Glycosylation	280	1	N-linked (GlcNAc...) Potential
	Glycosylation	292	1	N-linked (GlcNAc...) Potential
	Glycosylation	367	1	N-linked (GlcNAc...) Potential
	Glycosylation	427	1	N-linked (GlcNAc...) Potential
	Glycosylation	459	1	N-linked (GlcNAc...) Potential
	Disulfide bond	118 ↔ 522		By similarity
	Disulfide bond	122 ↔ 156		By similarity
	Disulfide bond	182 ↔ 193		By similarity
	Disulfide bond	406 ↔ 448		By similarity
Natural variations
	Alternative sequence	383 – 431	49	EGHPS…NTSIH → PVTGDHWHAEGELGCSFRTA SAAEEEPTYKLREKKRRKSP DCGRARWFL in isoform 2.		VSP_007881
	Alternative sequence	432 – 534	103	Missing in isoform 2.		VSP_022249
Experimental info
	Sequence conflict	60	1	D → A in AAA37261. Ref.3
	Sequence conflict	352	1	T → S in BAE34455. Ref.1
	Sequence conflict	352	1	T → S in CAA63067. Ref.4
	Sequence conflict	467	1	G → D in CAI84992. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 9, 2007. Version 3. Checksum: 78DAB2D65C71E97D	FASTA	534	59,647
	10 20 30 40 50 60 MGKLSPCTGR SRPGGPGPQL PLLLLLLQLL LLLLSPARAS GATQPPHVVF VLADDLGWND 70 80 90 100 110 120 LGFHGSVIRT PHLDALAAGG VVLDNYYVQP LCTPSRSQLL TGRYQIHLGL QHYLIMTCQP 130 140 150 160 170 180 SCVPLDEKLL PQLLKEAGYA THMVGKWHLG MYRKECLPTR RGFDTYFGYL LGSEDYYTHE 190 200 210 220 230 240 ACAPIESLNG TRCALDLRDG EEPAKEYNNI YSTNIFTKRA TTVIANHPPE KPLFLYLAFQ 250 260 270 280 290 300 SVHDPLQVPE EYMEPYGFIQ DKHRRIYAGM VSLMDEAVGN VTKALKSHGL WNNTVFIFST 310 320 330 340 350 360 DNGGQTRSGG NNWPLRGRKG TLWEGGIRGT GFVASPLLKQ KGVKSRELMH ITDWLPTLVD 370 380 390 400 410 420 LAGGSTNGTK PLDGFNMWKT ISEGHPSPRV ELLHNIDQDF FDGLPCPGKN MTPAKDDSFP 430 440 450 460 470 480 LEHSAFNTSI HAGIRYKNWK LLTGHPGCGY WFPPPSQSNV SEIPPVGPPT KTLWLFDINQ 490 500 510 520 530 DPEERHDVSR EHPHIVQNLL SRLQYYHEHS VPSHFPPLDP RCDPKSTGVW SPWM
	Isoform 2 [UniParc]. Checksum: E3C8BBE3E0B7C6D5	FASTA	431	47,934
	10 20 30 40 50 60 MGKLSPCTGR SRPGGPGPQL PLLLLLLQLL LLLLSPARAS GATQPPHVVF VLADDLGWND 70 80 90 100 110 120 LGFHGSVIRT PHLDALAAGG VVLDNYYVQP LCTPSRSQLL TGRYQIHLGL QHYLIMTCQP 130 140 150 160 170 180 SCVPLDEKLL PQLLKEAGYA THMVGKWHLG MYRKECLPTR RGFDTYFGYL LGSEDYYTHE 190 200 210 220 230 240 ACAPIESLNG TRCALDLRDG EEPAKEYNNI YSTNIFTKRA TTVIANHPPE KPLFLYLAFQ 250 260 270 280 290 300 SVHDPLQVPE EYMEPYGFIQ DKHRRIYAGM VSLMDEAVGN VTKALKSHGL WNNTVFIFST 310 320 330 340 350 360 DNGGQTRSGG NNWPLRGRKG TLWEGGIRGT GFVASPLLKQ KGVKSRELMH ITDWLPTLVD 370 380 390 400 410 420 LAGGSTNGTK PLDGFNMWKT ISPVTGDHWH AEGELGCSFR TASAAEEEPT YKLREKKRRK 430 SPDCGRARWF L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Inner ear and Thymus.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The sulfatase gene family: cross-species PCR cloning using the MOPAC technique." Grompe M., Pieretti M., Caskey C.T., Ballabio A. Genomics 12:755-760(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 58-90 (ISOFORM 1).
[4]	"Targeted disruption of the arylsulfatase B gene results in mice resembling the phenotype of mucopolysaccharidosis VI." Evers M., Saftig P., Schmidt P., Hafner A., McLoghlin D.B., Schmahl W., Hess B., von Figura K., Peters C. Proc. Natl. Acad. Sci. U.S.A. 93:8214-8219(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 137-388 (ISOFORM 1).
[5]	"Sulfatases and sulfatase modifying factors: an exclusive and promiscuous relationship." Sardiello M., Annunziata I., Roma G., Ballabio A. Hum. Mol. Genet. 14:3203-3217(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK083309 mRNA. Translation: BAC38859.1. AK154098 mRNA. Translation: BAE32375.1. AK158312 mRNA. Translation: BAE34455.1. AC131739 Genomic DNA. No translation available. AC136976 Genomic DNA. No translation available. M82877 mRNA. Translation: AAA37261.1. X92096 mRNA. Translation: CAA63067.1. BN000746 mRNA. Translation: CAI84992.1.
IPI	IPI00406459. IPI00652358.
RefSeq	NP_033842.3. NM_009712.3.
UniGene	Mm.300178. Mm.472255.
3D structure databases
ProteinModelPortal	P50429.
SMR	P50429. Positions 43-534.
ModBase	Search...
PTM databases
PhosphoSite	P50429.
Proteomic databases
PaxDb	P50429.
PRIDE	P50429.
Protocols and materials databases
DNASU	11881.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000091403; ENSMUSP00000088964; ENSMUSG00000042082.
GeneID	11881.
KEGG	mmu:11881.
UCSC	uc007rlo.1. mouse. uc011zcv.1. mouse.
Organism-specific databases
CTD	411.
MGI	MGI:88075. Arsb.
Phylogenomic databases
eggNOG	COG3119.
GeneTree	ENSGT00560000077076.
HOGENOM	HOG000135354.
HOVERGEN	HBG004282.
InParanoid	P50429.
KO	K01135.
OrthoDB	EOG4DV5M0.
Enzyme and pathway databases
SABIO-RK	P50429.
Gene expression databases
Bgee	P50429.
CleanEx	MM_ARSB.
Genevestigator	P50429.
GermOnline	ENSMUSG00000042093. Mus musculus.
Family and domain databases
Gene3D	3.40.720.10. 1 hit.
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR017850. Alkaline_phosphatase_core. IPR000917. Sulfatase. IPR024607. Sulfatase_CS. [Graphical view]
Pfam	PF00884. Sulfatase. 1 hit. [Graphical view]
SUPFAM	SSF53649. Alkaline_phosphatase_core. 1 hit.
PROSITE	PS00523. SULFATASE_1. 1 hit. PS00149. SULFATASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	279911.
SOURCE	Search...

Entry information

Entry name

ARSB_MOUSE

Accession

Primary (citable) accession number: P50429
Secondary accession number(s): Q32KJ1 , Q3TYV7, Q3U4Q6, Q8C404

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 9, 2007
Last modified:	April 3, 2013
This is version 114 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program