Reviewed, UniProtKB/Swiss-Prot P50429 (ARSB_MOUSE)

Names and origin

Protein names

Recommended name:
    Arylsulfatase B
      Short name=ASB
    EC=3.1.6.12
Alternative name(s):
    N-acetylgalactosamine-4-sulfatase
      Short name=G4S

Gene names

Name:	Arsb
Synonyms:	As1, As1-s

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of the 4-sulfate groups of the N-acetyl-D-galactosamine 4-sulfate units of chondroitin sulfate and dermatan sulfate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Lysosome.
Post-translational modification	The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity By similarity.
Sequence similarities	Belongs to the sulfatase family.

Ontologies

Keywords
Cellular component	Lysosome
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylgalactosamine-4-sulfatase activity Inferred from electronic annotation. Source: EC arylsulfatase activity Inferred from direct assay. Source: MGI calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P50429-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P50429-2)

The sequence of this isoform differs from the canonical sequence as follows:
     383-431: EGHPSPRVEL...EHSAFNTSIH → PVTGDHWHAE...PDCGRARWFL
     432-534: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 41	41	Potential
	Chain	42 – 534	493	Arylsulfatase B		PRO_0000033423
Sites
	Active site	148	1	By similarity
	Metal binding	54	1	Calcium By similarity
	Metal binding	55	1	Calcium By similarity
	Metal binding	92	1	Calcium; via 3-oxoalanine By similarity
	Metal binding	301	1	Calcium By similarity
	Metal binding	302	1	Calcium By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	319	1	Substrate By similarity
Amino acid modifications
	Modified residue	92	1	3-oxoalanine (Cys) By similarity
	Glycosylation	189	1	N-linked (GlcNAc...) Potential
	Glycosylation	280	1	N-linked (GlcNAc...) Potential
	Glycosylation	292	1	N-linked (GlcNAc...) Potential
	Glycosylation	367	1	N-linked (GlcNAc...) Potential
	Glycosylation	427	1	N-linked (GlcNAc...) Potential
	Glycosylation	459	1	N-linked (GlcNAc...) Potential
	Disulfide bond	118 ↔ 522		By similarity
	Disulfide bond	122 ↔ 156		By similarity
	Disulfide bond	182 ↔ 193		By similarity
	Disulfide bond	406 ↔ 448		By similarity
Natural variations
	Alternative sequence	383 – 431	49	EGHPS…NTSIH → PVTGDHWHAEGELGCSFRTA SAAEEEPTYKLREKKRRKSP DCGRARWFL in isoform 2.		VSP_007881
	Alternative sequence	432 – 534	103	Missing in isoform 2.		VSP_022249
Experimental info
	Sequence conflict	60	1	D → A in AAA37261. Ref.3
	Sequence conflict	352	1	T → S in BAE34455. Ref.1
	Sequence conflict	352	1	T → S in CAA63067. Ref.4
	Sequence conflict	467	1	G → D in CAI84992. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 9, 2007. Version 3. Checksum: 78DAB2D65C71E97D	FASTA	534	59,647
	10 20 30 40 50 60 MGKLSPCTGR SRPGGPGPQL PLLLLLLQLL LLLLSPARAS GATQPPHVVF VLADDLGWND 70 80 90 100 110 120 LGFHGSVIRT PHLDALAAGG VVLDNYYVQP LCTPSRSQLL TGRYQIHLGL QHYLIMTCQP 130 140 150 160 170 180 SCVPLDEKLL PQLLKEAGYA THMVGKWHLG MYRKECLPTR RGFDTYFGYL LGSEDYYTHE 190 200 210 220 230 240 ACAPIESLNG TRCALDLRDG EEPAKEYNNI YSTNIFTKRA TTVIANHPPE KPLFLYLAFQ 250 260 270 280 290 300 SVHDPLQVPE EYMEPYGFIQ DKHRRIYAGM VSLMDEAVGN VTKALKSHGL WNNTVFIFST 310 320 330 340 350 360 DNGGQTRSGG NNWPLRGRKG TLWEGGIRGT GFVASPLLKQ KGVKSRELMH ITDWLPTLVD 370 380 390 400 410 420 LAGGSTNGTK PLDGFNMWKT ISEGHPSPRV ELLHNIDQDF FDGLPCPGKN MTPAKDDSFP 430 440 450 460 470 480 LEHSAFNTSI HAGIRYKNWK LLTGHPGCGY WFPPPSQSNV SEIPPVGPPT KTLWLFDINQ 490 500 510 520 530 DPEERHDVSR EHPHIVQNLL SRLQYYHEHS VPSHFPPLDP RCDPKSTGVW SPWM
	Isoform 2. Checksum: E3C8BBE3E0B7C6D5	FASTA	431	47,934
	10 20 30 40 50 60 MGKLSPCTGR SRPGGPGPQL PLLLLLLQLL LLLLSPARAS GATQPPHVVF VLADDLGWND 70 80 90 100 110 120 LGFHGSVIRT PHLDALAAGG VVLDNYYVQP LCTPSRSQLL TGRYQIHLGL QHYLIMTCQP 130 140 150 160 170 180 SCVPLDEKLL PQLLKEAGYA THMVGKWHLG MYRKECLPTR RGFDTYFGYL LGSEDYYTHE 190 200 210 220 230 240 ACAPIESLNG TRCALDLRDG EEPAKEYNNI YSTNIFTKRA TTVIANHPPE KPLFLYLAFQ 250 260 270 280 290 300 SVHDPLQVPE EYMEPYGFIQ DKHRRIYAGM VSLMDEAVGN VTKALKSHGL WNNTVFIFST 310 320 330 340 350 360 DNGGQTRSGG NNWPLRGRKG TLWEGGIRGT GFVASPLLKQ KGVKSRELMH ITDWLPTLVD 370 380 390 400 410 420 LAGGSTNGTK PLDGFNMWKT ISPVTGDHWH AEGELGCSFR TASAAEEEPT YKLREKKRRK 430 SPDCGRARWF L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Inner ear and Thymus.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The sulfatase gene family: cross-species PCR cloning using the MOPAC technique." Grompe M., Pieretti M., Caskey C.T., Ballabio A. Genomics 12:755-760(1992) [PubMed: 1572648] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 58-90 (ISOFORM 1).
[4]	"Targeted disruption of the arylsulfatase B gene results in mice resembling the phenotype of mucopolysaccharidosis VI." Evers M., Saftig P., Schmidt P., Hafner A., McLoghlin D.B., Schmahl W., Hess B., von Figura K., Peters C. Proc. Natl. Acad. Sci. U.S.A. 93:8214-8219(1996) [PubMed: 8710849] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 137-388 (ISOFORM 1).
[5]	"Sulfatases and sulfatase modifying factors: an exclusive and promiscuous relationship." Sardiello M., Annunziata I., Roma G., Ballabio A. Hum. Mol. Genet. 14:3203-3217(2005) [PubMed: 16174644] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK083309 mRNA. Translation: BAC38859.1. AK154098 mRNA. Translation: BAE32375.1. AK158312 mRNA. Translation: BAE34455.1. AC131739 Genomic DNA. No translation available. AC136976 Genomic DNA. No translation available. M82877 mRNA. Translation: AAA37261.1. X92096 mRNA. Translation: CAA63067.1. BN000746 mRNA. Translation: CAI84992.1.
IPI	IPI00406459. IPI00652358.
RefSeq	NP_033842.3.
UniGene	Mm.300178 Mm.472255
3D structure databases
HSSP	HSSP built from PDB template 1FSU based on UniProtKB P15848.
ModBase	Search...
Protein-protein interaction databases
STRING	P50429.
Proteomic databases
PRIDE	P50429.
Genome annotation databases
Ensembl	ENSMUST00000091403; ENSMUSP00000088964; ENSMUSG00000042082; Mus musculus. [Genome view] ENSMUST00000109482; ENSMUSP00000105108; ENSMUSG00000042082; Mus musculus. [Genome view]
GeneID	11881.
KEGG	mmu:11881.
UCSC	uc007rlo.1. mouse.
Organism-specific databases
CTD	11881.
MGI	MGI:88075. Arsb.
Phylogenomic databases
HOGENOM	P50429.
HOVERGEN	P50429.
OMA	SFRTASA.
Enzyme and pathway databases
BRENDA	3.1.6.12. 244.
Gene expression databases
ArrayExpress	P50429.
Bgee	P50429.
CleanEx	MM_ARSB.
Genevestigator	P50429.
GermOnline	ENSMUSG00000042093. Mus musculus.
Family and domain databases
InterPro	IPR000917. Sulfatase. [Graphical view]
Pfam	PF00884. Sulfatase. 1 hit. [Graphical view]
PROSITE	PS00523. SULFATASE_1. 1 hit. PS00149. SULFATASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	279911.
SOURCE	Search...

Entry information

Entry name

ARSB_MOUSE

Accession

Primary (citable) accession number: P50429
Secondary accession number(s): Q32KJ1 , Q3TYV7, Q3U4Q6, Q8C404

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 9, 2007
Last modified:	November 3, 2009
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)