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Protein

Arylsulfatase A

Gene

Arsa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes cerebroside sulfate.

Catalytic activityi

A cerebroside 3-sulfate + H2O = a cerebroside + sulfate.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi28CalciumBy similarity1
Metal bindingi29CalciumBy similarity1
Metal bindingi68Calcium; via 3-oxoalanineBy similarity1
Binding sitei122SubstrateBy similarity1
Active sitei124By similarity1
Binding sitei149SubstrateBy similarity1
Binding sitei228SubstrateBy similarity1
Metal bindingi280CalciumBy similarity1
Metal bindingi281CalciumBy similarity1
Binding sitei301SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.
R-MMU-1663150. The activation of arylsulfatases.
R-MMU-6798695. Neutrophil degranulation.
SABIO-RKP50428.

Chemistry databases

SwissLipidsiSLP:000000914.

Names & Taxonomyi

Protein namesi
Recommended name:
Arylsulfatase A (EC:3.1.6.8)
Short name:
ASA
Alternative name(s):
Cerebroside-sulfatase
Gene namesi
Name:Arsa
Synonyms:As2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:88077. Arsa.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000003342018 – 506Arylsulfatase AAdd BLAST489

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei683-oxoalanine (Cys)By similarity1
Disulfide bondi155 ↔ 171By similarity
Glycosylationi157N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi160 ↔ 167By similarity
Glycosylationi183N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi299 ↔ 413By similarity
Glycosylationi349N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi487 ↔ 499By similarity
Disulfide bondi488 ↔ 501By similarity
Disulfide bondi492 ↔ 498By similarity

Post-translational modificationi

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP50428.
MaxQBiP50428.
PaxDbiP50428.
PeptideAtlasiP50428.
PRIDEiP50428.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022620.
CleanExiMM_ARSA.
ExpressionAtlasiP50428. baseline and differential.
GenevisibleiP50428. MM.

Interactioni

Subunit structurei

Homodimer at neutral pH and homooctamer at acidic pH. Exists both as a single chain of 58 kDa (component A) or as a chain of 50 kDa (component B) linked by disulfide bond(s) to a 7 kDa chain (component C). Interacts with SUMF1 (By similarity).By similarity

Protein-protein interaction databases

IntActiP50428. 2 interactors.
MINTiMINT-4128568.
STRINGi10090.ENSMUSP00000127646.

Structurei

3D structure databases

ProteinModelPortaliP50428.
SMRiP50428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3867. Eukaryota.
COG3119. LUCA.
GeneTreeiENSGT00760000119062.
HOGENOMiHOG000135352.
HOVERGENiHBG004283.
InParanoidiP50428.
KOiK01134.
OMAiFFYSAYP.
OrthoDBiEOG091G07AL.
PhylomeDBiP50428.
TreeFamiTF314186.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALGTLFLAL AAGLSTASPP NILLIFADDL GYGDLGSYGH PSSTTPNLDQ
60 70 80 90 100
LAEGGLRFTD FYVPVSLCTP SRAALLTGRL PVRSGMYPGV LGPSSQGGLP
110 120 130 140 150
LEEVTLAEVL AARGYLTGMA GKWHLGVGPE GAFLPPHQGF HRFLGIPYSH
160 170 180 190 200
DQGPCQNLTC FPPDIPCKGG CDQGLVPIPL LANLTVEAQP PWLPGLEARY
210 220 230 240 250
VSFSRDLMAD AQRQGRPFFL YYASHHTHYP QFSGQSFTKR SGRGPFGDSL
260 270 280 290 300
MELDGAVGAL MTTVGDLGLL EETLVIFTAD NGPELMRMSN GGCSGLLRCG
310 320 330 340 350
KGTTFEGGVR EPALVYWPGH ITPGVTHELA SSLDLLPTLA ALTGAPLPNV
360 370 380 390 400
TLDGVDISPL LLGTGKSPRK SVFFYPPYPD EIHGVFAVRN GKYKAHFFTQ
410 420 430 440 450
GSAHSDTTSD PACHAANRLT AHEPPLLYDL SQDPGENYNV LESIEGVSPE
460 470 480 490 500
ALQALKHIQL LKAQYDAAMT FGPSQIAKGE DPALQICCQP SCTPHPVCCH

CPGSQS
Length:506
Mass (Da):53,748
Last modified:July 27, 2011 - v2
Checksum:iA12DFA369D65B1AD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85G → A in CAA51702 (PubMed:7910580).Curated1
Sequence conflicti85G → A in CAA51703 (PubMed:7910580).Curated1
Sequence conflicti104V → L in CAA51702 (PubMed:7910580).Curated1
Sequence conflicti104V → L in CAA51703 (PubMed:7910580).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73230 mRNA. Translation: CAA51702.1.
X73231 Genomic DNA. Translation: CAA51703.1.
AK004540 mRNA. Translation: BAB23356.1.
AK132501 mRNA. Translation: BAE21207.1.
CH466550 Genomic DNA. Translation: EDL04336.1.
BC011284 mRNA. Translation: AAH11284.1.
BC098075 mRNA. Translation: AAH98075.1.
M82876 mRNA. Translation: AAA37260.1.
CCDSiCCDS27753.1.
PIRiA54190.
RefSeqiNP_033843.2. NM_009713.4.
UniGeneiMm.620.

Genome annotation databases

EnsembliENSMUST00000165199; ENSMUSP00000127646; ENSMUSG00000022620.
GeneIDi11883.
KEGGimmu:11883.
UCSCiuc007xgy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73230 mRNA. Translation: CAA51702.1.
X73231 Genomic DNA. Translation: CAA51703.1.
AK004540 mRNA. Translation: BAB23356.1.
AK132501 mRNA. Translation: BAE21207.1.
CH466550 Genomic DNA. Translation: EDL04336.1.
BC011284 mRNA. Translation: AAH11284.1.
BC098075 mRNA. Translation: AAH98075.1.
M82876 mRNA. Translation: AAA37260.1.
CCDSiCCDS27753.1.
PIRiA54190.
RefSeqiNP_033843.2. NM_009713.4.
UniGeneiMm.620.

3D structure databases

ProteinModelPortaliP50428.
SMRiP50428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP50428. 2 interactors.
MINTiMINT-4128568.
STRINGi10090.ENSMUSP00000127646.

Chemistry databases

SwissLipidsiSLP:000000914.

Proteomic databases

EPDiP50428.
MaxQBiP50428.
PaxDbiP50428.
PeptideAtlasiP50428.
PRIDEiP50428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000165199; ENSMUSP00000127646; ENSMUSG00000022620.
GeneIDi11883.
KEGGimmu:11883.
UCSCiuc007xgy.2. mouse.

Organism-specific databases

CTDi410.
MGIiMGI:88077. Arsa.

Phylogenomic databases

eggNOGiKOG3867. Eukaryota.
COG3119. LUCA.
GeneTreeiENSGT00760000119062.
HOGENOMiHOG000135352.
HOVERGENiHBG004283.
InParanoidiP50428.
KOiK01134.
OMAiFFYSAYP.
OrthoDBiEOG091G07AL.
PhylomeDBiP50428.
TreeFamiTF314186.

Enzyme and pathway databases

ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.
R-MMU-1663150. The activation of arylsulfatases.
R-MMU-6798695. Neutrophil degranulation.
SABIO-RKP50428.

Miscellaneous databases

PROiP50428.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022620.
CleanExiMM_ARSA.
ExpressionAtlasiP50428. baseline and differential.
GenevisibleiP50428. MM.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARSA_MOUSE
AccessioniPrimary (citable) accession number: P50428
Secondary accession number(s): Q9DC66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.