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P50428

- ARSA_MOUSE

UniProt

P50428 - ARSA_MOUSE

Protein

Arylsulfatase A

Gene

Arsa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Hydrolyzes cerebroside sulfate.

    Catalytic activityi

    A cerebroside 3-sulfate + H2O = a cerebroside + sulfate.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi28 – 281CalciumBy similarity
    Metal bindingi29 – 291CalciumBy similarity
    Metal bindingi68 – 681Calcium; via 3-oxoalanineBy similarity
    Binding sitei122 – 1221SubstrateBy similarity
    Active sitei124 – 1241By similarity
    Binding sitei149 – 1491SubstrateBy similarity
    Binding sitei228 – 2281SubstrateBy similarity
    Metal bindingi280 – 2801CalciumBy similarity
    Metal bindingi281 – 2811CalciumBy similarity
    Binding sitei301 – 3011SubstrateBy similarity

    GO - Molecular functioni

    1. arylsulfatase activity Source: Ensembl
    2. calcium ion binding Source: UniProtKB
    3. cerebroside-sulfatase activity Source: UniProtKB-EC
    4. sulfuric ester hydrolase activity Source: MGI

    GO - Biological processi

    1. autophagy Source: Ensembl
    2. binding of sperm to zona pellucida Source: MGI
    3. central nervous system development Source: Ensembl
    4. response to estrogen Source: Ensembl
    5. response to ethanol Source: Ensembl
    6. response to methylmercury Source: Ensembl
    7. response to nutrient Source: Ensembl
    8. response to pH Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198990. The activation of arylsulfatases.
    REACT_199008. Glycosphingolipid metabolism.
    SABIO-RKP50428.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arylsulfatase A (EC:3.1.6.8)
    Short name:
    ASA
    Alternative name(s):
    Cerebroside-sulfatase
    Gene namesi
    Name:Arsa
    Synonyms:As2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:88077. Arsa.

    Subcellular locationi

    GO - Cellular componenti

    1. acrosomal vesicle Source: Ensembl
    2. endosome Source: Ensembl
    3. extracellular space Source: Ensembl
    4. extrinsic component of external side of plasma membrane Source: Ensembl
    5. integral component of membrane Source: MGI
    6. lysosome Source: UniProtKB-SubCell
    7. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 506489Arylsulfatase APRO_0000033420Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei68 – 6813-oxoalanine (Cys)By similarity
    Disulfide bondi155 ↔ 171By similarity
    Glycosylationi157 – 1571N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi160 ↔ 167By similarity
    Glycosylationi183 – 1831N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi299 ↔ 413By similarity
    Glycosylationi349 – 3491N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi487 ↔ 499By similarity
    Disulfide bondi488 ↔ 501By similarity
    Disulfide bondi492 ↔ 498By similarity

    Post-translational modificationi

    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP50428.
    PaxDbiP50428.
    PRIDEiP50428.

    Expressioni

    Gene expression databases

    BgeeiP50428.
    CleanExiMM_ARSA.
    GenevestigatoriP50428.

    Interactioni

    Subunit structurei

    Homodimer at neutral pH and homooctamer at acidic pH. Exists both as a single chain of 58 kDa (component A) or as a chain of 50 kDa (component B) linked by disulfide bond(s) to a 7 kDa chain (component C). Interacts with SUMF1 By similarity.By similarity

    Protein-protein interaction databases

    IntActiP50428. 2 interactions.
    MINTiMINT-4128568.
    STRINGi10090.ENSMUSP00000023292.

    Structurei

    3D structure databases

    ProteinModelPortaliP50428.
    SMRiP50428. Positions 19-502.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the sulfatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3119.
    GeneTreeiENSGT00560000076940.
    HOGENOMiHOG000135352.
    HOVERGENiHBG004283.
    InParanoidiQ9DC66.
    KOiK01134.
    OMAiETMRMSH.
    OrthoDBiEOG7QZG9J.
    PhylomeDBiP50428.
    TreeFamiTF314186.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR000917. Sulfatase.
    IPR024607. Sulfatase_CS.
    [Graphical view]
    PfamiPF00884. Sulfatase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00523. SULFATASE_1. 1 hit.
    PS00149. SULFATASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50428-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALGTLFLAL AAGLSTASPP NILLIFADDL GYGDLGSYGH PSSTTPNLDQ    50
    LAEGGLRFTD FYVPVSLCTP SRAALLTGRL PVRSGMYPGV LGPSSQGGLP 100
    LEEVTLAEVL AARGYLTGMA GKWHLGVGPE GAFLPPHQGF HRFLGIPYSH 150
    DQGPCQNLTC FPPDIPCKGG CDQGLVPIPL LANLTVEAQP PWLPGLEARY 200
    VSFSRDLMAD AQRQGRPFFL YYASHHTHYP QFSGQSFTKR SGRGPFGDSL 250
    MELDGAVGAL MTTVGDLGLL EETLVIFTAD NGPELMRMSN GGCSGLLRCG 300
    KGTTFEGGVR EPALVYWPGH ITPGVTHELA SSLDLLPTLA ALTGAPLPNV 350
    TLDGVDISPL LLGTGKSPRK SVFFYPPYPD EIHGVFAVRN GKYKAHFFTQ 400
    GSAHSDTTSD PACHAANRLT AHEPPLLYDL SQDPGENYNV LESIEGVSPE 450
    ALQALKHIQL LKAQYDAAMT FGPSQIAKGE DPALQICCQP SCTPHPVCCH 500
    CPGSQS 506
    Length:506
    Mass (Da):53,748
    Last modified:July 27, 2011 - v2
    Checksum:iA12DFA369D65B1AD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti85 – 851G → A in CAA51702. (PubMed:7910580)Curated
    Sequence conflicti85 – 851G → A in CAA51703. (PubMed:7910580)Curated
    Sequence conflicti104 – 1041V → L in CAA51702. (PubMed:7910580)Curated
    Sequence conflicti104 – 1041V → L in CAA51703. (PubMed:7910580)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73230 mRNA. Translation: CAA51702.1.
    X73231 Genomic DNA. Translation: CAA51703.1.
    AK004540 mRNA. Translation: BAB23356.1.
    AK132501 mRNA. Translation: BAE21207.1.
    CH466550 Genomic DNA. Translation: EDL04336.1.
    BC011284 mRNA. Translation: AAH11284.1.
    BC098075 mRNA. Translation: AAH98075.1.
    M82876 mRNA. Translation: AAA37260.1.
    CCDSiCCDS27753.1.
    PIRiA54190.
    RefSeqiNP_033843.2. NM_009713.4.
    UniGeneiMm.620.

    Genome annotation databases

    EnsembliENSMUST00000165199; ENSMUSP00000127646; ENSMUSG00000022620.
    GeneIDi11883.
    KEGGimmu:11883.
    UCSCiuc007xgy.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73230 mRNA. Translation: CAA51702.1 .
    X73231 Genomic DNA. Translation: CAA51703.1 .
    AK004540 mRNA. Translation: BAB23356.1 .
    AK132501 mRNA. Translation: BAE21207.1 .
    CH466550 Genomic DNA. Translation: EDL04336.1 .
    BC011284 mRNA. Translation: AAH11284.1 .
    BC098075 mRNA. Translation: AAH98075.1 .
    M82876 mRNA. Translation: AAA37260.1 .
    CCDSi CCDS27753.1.
    PIRi A54190.
    RefSeqi NP_033843.2. NM_009713.4.
    UniGenei Mm.620.

    3D structure databases

    ProteinModelPortali P50428.
    SMRi P50428. Positions 19-502.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P50428. 2 interactions.
    MINTi MINT-4128568.
    STRINGi 10090.ENSMUSP00000023292.

    Proteomic databases

    MaxQBi P50428.
    PaxDbi P50428.
    PRIDEi P50428.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000165199 ; ENSMUSP00000127646 ; ENSMUSG00000022620 .
    GeneIDi 11883.
    KEGGi mmu:11883.
    UCSCi uc007xgy.2. mouse.

    Organism-specific databases

    CTDi 410.
    MGIi MGI:88077. Arsa.

    Phylogenomic databases

    eggNOGi COG3119.
    GeneTreei ENSGT00560000076940.
    HOGENOMi HOG000135352.
    HOVERGENi HBG004283.
    InParanoidi Q9DC66.
    KOi K01134.
    OMAi ETMRMSH.
    OrthoDBi EOG7QZG9J.
    PhylomeDBi P50428.
    TreeFami TF314186.

    Enzyme and pathway databases

    Reactomei REACT_198990. The activation of arylsulfatases.
    REACT_199008. Glycosphingolipid metabolism.
    SABIO-RK P50428.

    Miscellaneous databases

    NextBioi 279915.
    PROi P50428.
    SOURCEi Search...

    Gene expression databases

    Bgeei P50428.
    CleanExi MM_ARSA.
    Genevestigatori P50428.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR000917. Sulfatase.
    IPR024607. Sulfatase_CS.
    [Graphical view ]
    Pfami PF00884. Sulfatase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00523. SULFATASE_1. 1 hit.
    PS00149. SULFATASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: 129/Sv and C57BL/6J.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Lung and Skin.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor and Trophoblast stem cell.
    5. "The sulfatase gene family: cross-species PCR cloning using the MOPAC technique."
      Grompe M., Pieretti M., Caskey C.T., Ballabio A.
      Genomics 12:755-760(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-66.

    Entry informationi

    Entry nameiARSA_MOUSE
    AccessioniPrimary (citable) accession number: P50428
    Secondary accession number(s): Q9DC66
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3