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Protein

Steryl-sulfatase

Gene

Sts

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conversion of sulfated steroid precursors to estrogens during pregnancy.

Catalytic activityi

3-beta-hydroxyandrost-5-en-17-one 3-sulfate + H2O = 3-beta-hydroxyandrost-5-en-17-one + sulfate.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431CalciumBy similarity
Metal bindingi44 – 441CalciumBy similarity
Metal bindingi83 – 831Calcium; via 3-oxoalanineBy similarity
Active sitei144 – 1441By similarity
Metal bindingi350 – 3501CalciumBy similarity
Metal bindingi351 – 3511CalciumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid metabolism, Pregnancy, Steroid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Steryl-sulfatase (EC:3.1.6.2)
Alternative name(s):
Arylsulfatase C
Short name:
ASC
Steroid sulfatase
Steryl-sulfate sulfohydrolase
Gene namesi
Name:Sts
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:98438. Sts.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 192169LumenalBy similarityAdd
BLAST
Transmembranei193 – 21624HelicalBy similarityAdd
BLAST
Topological domaini217 – 2204CytoplasmicBy similarity
Transmembranei221 – 24222HelicalBy similarityAdd
BLAST
Topological domaini243 – 624382LumenalBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 624602Steryl-sulfatasePRO_0000033415Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi55 – 551N-linked (GlcNAc...)Sequence analysis
Modified residuei83 – 8313-oxoalanine (Cys)By similarity
Disulfide bondi149 ↔ 156By similarity
Disulfide bondi178 ↔ 250By similarity
Disulfide bondi454 ↔ 495By similarity
Glycosylationi465 – 4651N-linked (GlcNAc...)Sequence analysis
Disulfide bondi487 ↔ 493By similarity

Post-translational modificationi

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP50427.
MaxQBiP50427.
PRIDEiP50427.

PTM databases

iPTMnetiP50427.
PhosphoSiteiP50427.
SwissPalmiP50427.

Expressioni

Gene expression databases

CleanExiMM_STS.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi203559. 1 interaction.
IntActiP50427. 2 interactions.
MINTiMINT-1851563.

Structurei

3D structure databases

ProteinModelPortaliP50427.
SMRiP50427. Positions 31-568.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG004283.
InParanoidiP50427.
KOiK01131.
PhylomeDBiP50427.

Family and domain databases

Gene3Di3.40.720.10. 2 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50427-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRPRPLLLA VMAATLADII LAADPAPAGP APRPPNFLLI MADDLGIGDL
60 70 80 90 100
GCYGNKTLRT PHLDRLAREG VKLTQHLAAA PLCTPSRAAF LTGRYPPRSG
110 120 130 140 150
MAAHGRVGVY LFTASSGGLP PSEVTMARLL KGRGYATALI GKWHLGLSCR
160 170 180 190 200
GATDFCHHPL RHGFDRFLGV PTTNLRDCRP GAGTVFGPAL RVFAAGPLAA
210 220 230 240 250
LGASLAAMAA ARWAGLARVP GWALAGTAAA MLAVGGPRSA SCLGFRPANC
260 270 280 290 300
FLMDDLAVAQ RPTDYGGLTR RLADEAALFL RRNRARPFLL FLSFLHVHTA
310 320 330 340 350
HFADPGFAGR SLHGAYGDSV EEMDWGVGRV LAALDELGLA RETLVYFTSD
360 370 380 390 400
HGAHVEELGP RGERMGGSNG VFRGGKGNNW EGGVRVPCLV RWPRELSPGR
410 420 430 440 450
VVAEPTSLMD VFPTVARLAG AELPGDRVID GRDLMPLLRG DAQRSEHEFL
460 470 480 490 500
FHYCNAYLQA VRWHNGSAVW KAFYFTPNFA PAGANGCFST HVCLCAGPAH
510 520 530 540 550
VTAHDPPLLF DLTRDPGERR PLTPEAEPRH REVLDAIDAA ARAHRARLRP
560 570 580 590 600
APDQLAPRHL MWKPWLQLWG GGGAGGGAGA QDDSGHAHGD GSHAHDDPGH
610 620
AQDRGDDDAH YGGHATTRTQ ATPR
Length:624
Mass (Da):66,591
Last modified:October 1, 1996 - v1
Checksum:i025C0CF8659D0A9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37545 mRNA. Translation: AAB09308.1.
RefSeqiNP_033319.1. NM_009293.1.
UniGeneiMm.423011.

Genome annotation databases

GeneIDi20905.
KEGGimmu:20905.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37545 mRNA. Translation: AAB09308.1.
RefSeqiNP_033319.1. NM_009293.1.
UniGeneiMm.423011.

3D structure databases

ProteinModelPortaliP50427.
SMRiP50427. Positions 31-568.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203559. 1 interaction.
IntActiP50427. 2 interactions.
MINTiMINT-1851563.

PTM databases

iPTMnetiP50427.
PhosphoSiteiP50427.
SwissPalmiP50427.

Proteomic databases

EPDiP50427.
MaxQBiP50427.
PRIDEiP50427.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20905.
KEGGimmu:20905.

Organism-specific databases

CTDi412.
MGIiMGI:98438. Sts.

Phylogenomic databases

HOVERGENiHBG004283.
InParanoidiP50427.
KOiK01131.
PhylomeDBiP50427.

Miscellaneous databases

PROiP50427.
SOURCEiSearch...

Gene expression databases

CleanExiMM_STS.

Family and domain databases

Gene3Di3.40.720.10. 2 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the mouse pseudoautosomal steroid sulphatase gene (Sts)."
    Salido E.C., Li X.M., Yen P.H., Martin N., Mohandas T.K., Shapiro L.J.
    Nat. Genet. 13:83-86(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver, Lung, Spleen and Testis.

Entry informationi

Entry nameiSTS_MOUSE
AccessioniPrimary (citable) accession number: P50427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.