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P50405 (PSPB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pulmonary surfactant-associated protein B

Short name=SP-B
Alternative name(s):
Pulmonary surfactant-associated proteolipid SPL(Phe)
Gene names
Name:Sftpb
Synonyms:Sftp3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

Subunit structure

Homodimer; disulfide-linked.

Subcellular location

Secretedextracellular spacesurface film.

Miscellaneous

Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Sequence similarities

Contains 1 saposin A-type domain.

Contains 3 saposin B-type domains.

Ontologies

Keywords
   Biological processGaseous exchange
   Cellular componentSecreted
Surface film
   DomainRepeat
Signal
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processrespiratory gaseous exchange

Inferred from electronic annotation. Source: UniProtKB-KW

sphingolipid metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

lysosome

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Propeptide23 – 191169
PRO_0000031650
Chain192 – 27079Pulmonary surfactant-associated protein B
PRO_0000031651
Propeptide271 – 377107
PRO_0000031652

Regions

Domain24 – 6441Saposin A-type
Domain64 – 14683Saposin B-type 1
Domain195 – 27278Saposin B-type 2
Domain291 – 36676Saposin B-type 3

Amino acid modifications

Glycosylation3071N-linked (GlcNAc...) Potential
Disulfide bond68 ↔ 142 By similarity
Disulfide bond71 ↔ 136 By similarity
Disulfide bond99 ↔ 111 By similarity
Disulfide bond199 ↔ 268 By similarity
Disulfide bond202 ↔ 262 By similarity
Disulfide bond226 ↔ 237 By similarity
Disulfide bond239Interchain By similarity
Disulfide bond295 ↔ 362 By similarity
Disulfide bond298 ↔ 356 By similarity
Disulfide bond321 ↔ 331 By similarity

Sequences

Sequence LengthMass (Da)Tools
P50405 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: CB687A82BA3FC56C

FASTA37741,728
        10         20         30         40         50         60 
MAKSHLLQWL LLLPTLCCPG AAITSASSLE CAQGPQFWCQ SLEHAVQCRA LGHCLQEVWG 

        70         80         90        100        110        120 
HAGANDLCQE CEDIVHLLTK MTKEDAFQEA IRKFLEQECD ILPLKLLVPR CRQVLDVYLP 

       130        140        150        160        170        180 
LVIDYFQSQI NPKAICNHVG LCPRGQAKPE QNPGMPDAVP NPLLDKLVLP VLPGALLARP 

       190        200        210        220        230        240 
GPHTQDFSEQ QLPIPLPFCW LCRTLIKRVQ AVIPKGVLAV AVSQVCHVVP LVVGGICQCL 

       250        260        270        280        290        300 
AERYTVLLLD ALLGRVVPQL VCGLVLRCST EDAMGPALPA VEPLIEEWPL QDTECHFCKS 

       310        320        330        340        350        360 
VINQAWNTSE QAMPQAMHQA CLRFWLDRQK CEQFVEQHMP QLLALVPRSQ DAHITCQALG 

       370 
VCEAPASPLQ CFQTPHL 

« Hide

References

[1]"Structure and function of the mouse surfactant protein B gene."
Bruno M.A., Bohinski R.J., Carter J.E., Foss K.A., Whitsett J.A.
Am. J. Physiol. 268:L381-L389(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DBA/2J.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S78114 Genomic DNA. Translation: AAB34846.2.
UniGeneMm.46033.

3D structure databases

ProteinModelPortalP50405.
SMRP50405. Positions 40-142, 198-269, 293-363.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP50405. 1 interaction.
MINTMINT-4109008.
STRING10090.ENSMUSP00000066805.

Proteomic databases

PaxDbP50405.
PRIDEP50405.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCuc009cid.1. mouse.

Organism-specific databases

MGIMGI:109516. Sftpb.

Phylogenomic databases

eggNOGNOG286450.
HOGENOMHOG000115745.
HOVERGENHBG006905.
InParanoidP50405.
PhylomeDBP50405.

Gene expression databases

ArrayExpressP50405.
BgeeP50405.
CleanExMM_SFTPB.
GenevestigatorP50405.

Family and domain databases

Gene3D1.10.225.10. 3 hits.
InterProIPR003119. SapA.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR008139. SaposinB.
[Graphical view]
PfamPF02199. SapA. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 2 hits.
[Graphical view]
PRINTSPR01797. SAPOSIN.
SMARTSM00162. SAPA. 1 hit.
SM00741. SapB. 3 hits.
[Graphical view]
SUPFAMSSF47862. SSF47862. 3 hits.
PROSITEPS51110. SAP_A. 1 hit.
PS50015. SAP_B. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio298318.
PROP50405.
SOURCESearch...

Entry information

Entry namePSPB_MOUSE
AccessionPrimary (citable) accession number: P50405
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot