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Protein

Pulmonary surfactant-associated protein B

Gene

Sftpb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

GO - Biological processi

  1. respiratory gaseous exchange Source: UniProtKB-KW
  2. sphingolipid metabolic process Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Gaseous exchange

Names & Taxonomyi

Protein namesi
Recommended name:
Pulmonary surfactant-associated protein B
Short name:
SP-B
Alternative name(s):
Pulmonary surfactant-associated proteolipid SPL(Phe)
Gene namesi
Name:Sftpb
Synonyms:Sftp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:109516. Sftpb.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. extracellular space Source: UniProtKB-SubCell
  3. lysosome Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted, Surface film

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Propeptidei23 – 191169PRO_0000031650Add
BLAST
Chaini192 – 27079Pulmonary surfactant-associated protein BPRO_0000031651Add
BLAST
Propeptidei271 – 377107PRO_0000031652Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi68 ↔ 142PROSITE-ProRule annotation
Disulfide bondi71 ↔ 136PROSITE-ProRule annotation
Disulfide bondi99 ↔ 111PROSITE-ProRule annotation
Disulfide bondi199 ↔ 268PROSITE-ProRule annotation
Disulfide bondi202 ↔ 262PROSITE-ProRule annotation
Disulfide bondi226 ↔ 237PROSITE-ProRule annotation
Disulfide bondi239 – 239InterchainPROSITE-ProRule annotation
Disulfide bondi295 ↔ 362PROSITE-ProRule annotation
Disulfide bondi298 ↔ 356PROSITE-ProRule annotation
Glycosylationi307 – 3071N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi321 ↔ 331PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP50405.
PaxDbiP50405.
PRIDEiP50405.

Expressioni

Gene expression databases

BgeeiP50405.
CleanExiMM_SFTPB.
GenevestigatoriP50405.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

Protein-protein interaction databases

IntActiP50405. 1 interaction.
MINTiMINT-4109008.
STRINGi10090.ENSMUSP00000066805.

Structurei

3D structure databases

SMRiP50405. Positions 68-142.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 6441Saposin A-typePROSITE-ProRule annotationAdd
BLAST
Domaini64 – 14683Saposin B-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini195 – 27278Saposin B-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini291 – 36676Saposin B-type 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 saposin A-type domain.PROSITE-ProRule annotation
Contains 3 saposin B-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG286450.
HOGENOMiHOG000115745.
HOVERGENiHBG006905.
InParanoidiP50405.
PhylomeDBiP50405.

Family and domain databases

Gene3Di1.10.225.10. 3 hits.
InterProiIPR003119. SapA.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR008139. SaposinB.
[Graphical view]
PfamiPF02199. SapA. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 2 hits.
[Graphical view]
PRINTSiPR01797. SAPOSIN.
SMARTiSM00162. SAPA. 1 hit.
SM00741. SapB. 3 hits.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 3 hits.
PROSITEiPS51110. SAP_A. 1 hit.
PS50015. SAP_B. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSHLLQWL LLLPTLCCPG AAITSASSLE CAQGPQFWCQ SLEHAVQCRA
60 70 80 90 100
LGHCLQEVWG HAGANDLCQE CEDIVHLLTK MTKEDAFQEA IRKFLEQECD
110 120 130 140 150
ILPLKLLVPR CRQVLDVYLP LVIDYFQSQI NPKAICNHVG LCPRGQAKPE
160 170 180 190 200
QNPGMPDAVP NPLLDKLVLP VLPGALLARP GPHTQDFSEQ QLPIPLPFCW
210 220 230 240 250
LCRTLIKRVQ AVIPKGVLAV AVSQVCHVVP LVVGGICQCL AERYTVLLLD
260 270 280 290 300
ALLGRVVPQL VCGLVLRCST EDAMGPALPA VEPLIEEWPL QDTECHFCKS
310 320 330 340 350
VINQAWNTSE QAMPQAMHQA CLRFWLDRQK CEQFVEQHMP QLLALVPRSQ
360 370
DAHITCQALG VCEAPASPLQ CFQTPHL
Length:377
Mass (Da):41,728
Last modified:October 1, 1996 - v1
Checksum:iCB687A82BA3FC56C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S78114 Genomic DNA. Translation: AAB34846.2.
RefSeqiNP_680088.1. NM_147779.2.
UniGeneiMm.46033.

Genome annotation databases

EnsembliENSMUST00000192630; ENSMUSP00000141551; ENSMUSG00000103879.
GeneIDi20388.
KEGGimmu:20388.
UCSCiuc009cid.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S78114 Genomic DNA. Translation: AAB34846.2.
RefSeqiNP_680088.1. NM_147779.2.
UniGeneiMm.46033.

3D structure databases

SMRiP50405. Positions 68-142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP50405. 1 interaction.
MINTiMINT-4109008.
STRINGi10090.ENSMUSP00000066805.

Proteomic databases

MaxQBiP50405.
PaxDbiP50405.
PRIDEiP50405.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000192630; ENSMUSP00000141551; ENSMUSG00000103879.
GeneIDi20388.
KEGGimmu:20388.
UCSCiuc009cid.1. mouse.

Organism-specific databases

CTDi6439.
MGIiMGI:109516. Sftpb.

Phylogenomic databases

eggNOGiNOG286450.
HOGENOMiHOG000115745.
HOVERGENiHBG006905.
InParanoidiP50405.
PhylomeDBiP50405.

Miscellaneous databases

NextBioi298318.
PROiP50405.
SOURCEiSearch...

Gene expression databases

BgeeiP50405.
CleanExiMM_SFTPB.
GenevestigatoriP50405.

Family and domain databases

Gene3Di1.10.225.10. 3 hits.
InterProiIPR003119. SapA.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR008139. SaposinB.
[Graphical view]
PfamiPF02199. SapA. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 2 hits.
[Graphical view]
PRINTSiPR01797. SAPOSIN.
SMARTiSM00162. SAPA. 1 hit.
SM00741. SapB. 3 hits.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 3 hits.
PROSITEiPS51110. SAP_A. 1 hit.
PS50015. SAP_B. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structure and function of the mouse surfactant protein B gene."
    Bruno M.A., Bohinski R.J., Carter J.E., Foss K.A., Whitsett J.A.
    Am. J. Physiol. 268:L381-L389(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DBA/2J.
    Tissue: Liver.

Entry informationi

Entry nameiPSPB_MOUSE
AccessioniPrimary (citable) accession number: P50405
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 29, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.