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P50401

- GUXA_CELFA

UniProt

P50401 - GUXA_CELFA

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Protein

Exoglucanase A

Gene

cbhA

Organism
Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This enzyme hydrolyzes 1,4-beta-D-glucosidic linkages of cellulose. Weak activity against carboxymethylcellulose, bacterial microcrystalline cellulose and barley beta-glucan. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration.

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei188 – 1881Proton donorPROSITE-ProRule annotation
Active sitei410 – 4101NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  1. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB-EC
  2. polysaccharide binding Source: InterPro

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCFIM590998:GJFK-1948-MONOMER.
SABIO-RKP50401.

Protein family/group databases

CAZyiCBM2. Carbohydrate-Binding Module Family 2.
GH6. Glycoside Hydrolase Family 6.

Names & Taxonomyi

Protein namesi
Recommended name:
Exoglucanase A (EC:3.2.1.91)
Alternative name(s):
1,4-beta-cellobiohydrolase A
CBP95
Exocellobiohydrolase A
Gene namesi
Name:cbhA
Ordered Locus Names:Celf_1925
OrganismiCellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547)
Taxonomic identifieri590998 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeCellulomonadaceaeCellulomonas
ProteomesiUP000008460: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 40401 PublicationAdd
BLAST
Chaini41 – 872832Exoglucanase APRO_0000007904Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi140 ↔ 202By similarity
Disulfide bondi374 ↔ 428By similarity
Disulfide bondi770 ↔ 869By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP50401.
SMRiP50401. Positions 674-762, 765-872.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini484 – 56986Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini579 – 66789Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini677 – 76589Fibronectin type-III 3PROSITE-ProRule annotationAdd
BLAST
Domaini763 – 872110CBM2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni41 – 477437CatalyticAdd
BLAST

Sequence similaritiesi

Contains 3 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

OMAiAYSFTVR.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
2.60.40.290. 1 hit.
3.20.20.40. 1 hit.
InterProiIPR016288. Beta_cellobiohydrolase.
IPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR018366. CBM2_CS.
IPR001919. Cellulose-bd_dom_fam2_bac.
IPR003961. Fibronectin_type3.
IPR001524. Glyco_hydro_6_CS.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00553. CBM_2. 1 hit.
PF00041. fn3. 3 hits.
PF01341. Glyco_hydro_6. 1 hit.
[Graphical view]
PRINTSiPR00733. GLHYDRLASE6.
SMARTiSM00637. CBD_II. 1 hit.
SM00060. FN3. 3 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
SSF49384. SSF49384. 1 hit.
SSF51989. SSF51989. 1 hit.
PROSITEiPS51173. CBM2. 1 hit.
PS00561. CBM2_A. 1 hit.
PS50853. FN3. 3 hits.
PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50401-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTLGKRAGV RRRVRAVATA ATATALVAVP LTTLATSASA APVHVDNPYA
60 70 80 90 100
GAVQYVNPTW AASVNAAAGR QSADPALAAK MRTVAGQPTA VWMDRISAIT
110 120 130 140 150
GNADGNGLKF HLDNAVAQQK AAGVPLVFNL VIYDLPGRDC FALASNGELP
160 170 180 190 200
ATDAGLARYK SEYIDPIADL LDNPEYESIR IAATIEPDSL PNLTTNISEP
210 220 230 240 250
ACQQAAPYYR QGVKYALDKL HAIPNVYNYI DIGHSGWLGW DSNAGPSATL
260 270 280 290 300
FAEVAKSTTA GFASIDGFVS DVANTTPLEE PLLSDSSLTI NNTPIRSSKF
310 320 330 340 350
YEWNFDFDEI DYTAHMHRLL VAAGFPSSIG MLVDTSRNGW GGPNRPTSIT
360 370 380 390 400
ASTDVNAYVD ANRVDRRVHR GAWCNPLGAG IGRFPEATPS GYAASHLDAF
410 420 430 440 450
VWIKPPGESD GASTDIPNDQ GKRFDRMCDP TFVSPKLNNQ LTGATPNAPL
460 470 480 490 500
AGQWFEEQFV TLVKNAYPVI GGTTPVEDLV APTVPTGLTA GTTTATSVPL
510 520 530 540 550
SWTASTDNVA VTGYDVYRGT TLVGTTAATS YTVTGLTPAT AYSFTVRAKD
560 570 580 590 600
AAGNVSAASA AAAATTQSGT VTDTTAPSVP AGLTAGTTTT TTVPLSWTAS
610 620 630 640 650
TDNAGGSGVA GYEVLRGTTV VGTTTATSYT VTGLTAGTTY SFSVRAKDVA
660 670 680 690 700
GNTSAASAAV SATTQTGTVV DTTAPSVPTG LTAGTTTTSS VPLTWTASTD
710 720 730 740 750
NAGGSGVAGY EVFNGTTRVA TVTSTSYTVT GLAADTAYSF TVKAKDVAGN
760 770 780 790 800
VSAASAAVSA RTQAATSGGC TVKYSASSWN TGFTGTVEVK NNGTAALNGW
810 820 830 840 850
TLGFSFADGQ KVSQGWSAEW SQSGTAVTAK NAPWNGTLAA GSSVSIGFNG
860 870
THNGTNTAPT AFTLNGVACT LG
Length:872
Mass (Da):89,301
Last modified:October 1, 1996 - v1
Checksum:i7883B407F995533B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25809 Unassigned DNA. Translation: AAC36898.1.
CP002666 Genomic DNA. Translation: AEE46055.1.
PIRiS49541.
RefSeqiWP_013771081.1. NC_015514.1.
YP_004453442.1. NC_015514.1.

Genome annotation databases

EnsemblBacteriaiAEE46055; AEE46055; Celf_1925.
GeneIDi10592708.
KEGGicfi:Celf_1925.
PATRICi54614786. VBICelFim18861_1929.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25809 Unassigned DNA. Translation: AAC36898.1 .
CP002666 Genomic DNA. Translation: AEE46055.1 .
PIRi S49541.
RefSeqi WP_013771081.1. NC_015514.1.
YP_004453442.1. NC_015514.1.

3D structure databases

ProteinModelPortali P50401.
SMRi P50401. Positions 674-762, 765-872.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM2. Carbohydrate-Binding Module Family 2.
GH6. Glycoside Hydrolase Family 6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AEE46055 ; AEE46055 ; Celf_1925 .
GeneIDi 10592708.
KEGGi cfi:Celf_1925.
PATRICi 54614786. VBICelFim18861_1929.

Phylogenomic databases

OMAi AYSFTVR.

Enzyme and pathway databases

BioCyci CFIM590998:GJFK-1948-MONOMER.
SABIO-RK P50401.

Family and domain databases

Gene3Di 2.60.40.10. 3 hits.
2.60.40.290. 1 hit.
3.20.20.40. 1 hit.
InterProi IPR016288. Beta_cellobiohydrolase.
IPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR018366. CBM2_CS.
IPR001919. Cellulose-bd_dom_fam2_bac.
IPR003961. Fibronectin_type3.
IPR001524. Glyco_hydro_6_CS.
IPR013783. Ig-like_fold.
[Graphical view ]
Pfami PF00553. CBM_2. 1 hit.
PF00041. fn3. 3 hits.
PF01341. Glyco_hydro_6. 1 hit.
[Graphical view ]
PRINTSi PR00733. GLHYDRLASE6.
SMARTi SM00637. CBD_II. 1 hit.
SM00060. FN3. 3 hits.
[Graphical view ]
SUPFAMi SSF49265. SSF49265. 2 hits.
SSF49384. SSF49384. 1 hit.
SSF51989. SSF51989. 1 hit.
PROSITEi PS51173. CBM2. 1 hit.
PS00561. CBM2_A. 1 hit.
PS50853. FN3. 3 hits.
PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cellobiohydrolase A (CbhA) from the cellulolytic bacterium Cellulomonas fimi is a beta-1,4-exocellobiohydrolase analogous to Trichoderma reesei CBH II."
    Meinke A., Gilkes N.R., Kwan E., Kilburn D.G., Warren R.A.J., Miller R.C. Jr.
    Mol. Microbiol. 12:413-422(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
  2. "Complete sequence of Cellulomonas fimi ATCC 484."
    US DOE Joint Genome Institute
    Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Mead D., Brumm P., Woyke T.
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
  3. "Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase."
    Meinke A., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
    J. Bacteriol. 175:1910-1918(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 41-58.
    Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.

Entry informationi

Entry nameiGUXA_CELFA
AccessioniPrimary (citable) accession number: P50401
Secondary accession number(s): F4GZL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 1, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3