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P50401 (GUXA_CELFA) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Exoglucanase A

EC=3.2.1.91
Alternative name(s):
1,4-beta-cellobiohydrolase A
CBP95
Exocellobiohydrolase A
Gene names
Name:cbhA
Ordered Locus Names:Celf_1925
OrganismCellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) [Complete proteome] [HAMAP]
Taxonomic identifier590998 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeCellulomonadaceaeCellulomonas

Protein attributes

Sequence length872 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme hydrolyzes 1,4-beta-D-glucosidic linkages of cellulose. Weak activity against carboxymethylcellulose, bacterial microcrystalline cellulose and barley beta-glucan. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration.

Catalytic activity

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sequence similarities

Belongs to the glycosyl hydrolase 6 (cellulase B) family.

Contains 1 CBM2 (carbohydrate binding type-2) domain.

Contains 3 fibronectin type-III domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4040 Ref.3
Chain41 – 872832Exoglucanase A
PRO_0000007904

Regions

Domain484 – 56986Fibronectin type-III 1
Domain579 – 66789Fibronectin type-III 2
Domain677 – 76589Fibronectin type-III 3
Domain763 – 872110CBM2
Region41 – 477437Catalytic

Sites

Active site1881Proton donor By similarity
Active site4101Nucleophile By similarity

Amino acid modifications

Disulfide bond140 ↔ 202 By similarity
Disulfide bond374 ↔ 428 By similarity
Disulfide bond770 ↔ 869 By similarity

Sequences

Sequence LengthMass (Da)Tools
P50401 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 7883B407F995533B

FASTA87289,301
        10         20         30         40         50         60 
MSTLGKRAGV RRRVRAVATA ATATALVAVP LTTLATSASA APVHVDNPYA GAVQYVNPTW 

        70         80         90        100        110        120 
AASVNAAAGR QSADPALAAK MRTVAGQPTA VWMDRISAIT GNADGNGLKF HLDNAVAQQK 

       130        140        150        160        170        180 
AAGVPLVFNL VIYDLPGRDC FALASNGELP ATDAGLARYK SEYIDPIADL LDNPEYESIR 

       190        200        210        220        230        240 
IAATIEPDSL PNLTTNISEP ACQQAAPYYR QGVKYALDKL HAIPNVYNYI DIGHSGWLGW 

       250        260        270        280        290        300 
DSNAGPSATL FAEVAKSTTA GFASIDGFVS DVANTTPLEE PLLSDSSLTI NNTPIRSSKF 

       310        320        330        340        350        360 
YEWNFDFDEI DYTAHMHRLL VAAGFPSSIG MLVDTSRNGW GGPNRPTSIT ASTDVNAYVD 

       370        380        390        400        410        420 
ANRVDRRVHR GAWCNPLGAG IGRFPEATPS GYAASHLDAF VWIKPPGESD GASTDIPNDQ 

       430        440        450        460        470        480 
GKRFDRMCDP TFVSPKLNNQ LTGATPNAPL AGQWFEEQFV TLVKNAYPVI GGTTPVEDLV 

       490        500        510        520        530        540 
APTVPTGLTA GTTTATSVPL SWTASTDNVA VTGYDVYRGT TLVGTTAATS YTVTGLTPAT 

       550        560        570        580        590        600 
AYSFTVRAKD AAGNVSAASA AAAATTQSGT VTDTTAPSVP AGLTAGTTTT TTVPLSWTAS 

       610        620        630        640        650        660 
TDNAGGSGVA GYEVLRGTTV VGTTTATSYT VTGLTAGTTY SFSVRAKDVA GNTSAASAAV 

       670        680        690        700        710        720 
SATTQTGTVV DTTAPSVPTG LTAGTTTTSS VPLTWTASTD NAGGSGVAGY EVFNGTTRVA 

       730        740        750        760        770        780 
TVTSTSYTVT GLAADTAYSF TVKAKDVAGN VSAASAAVSA RTQAATSGGC TVKYSASSWN 

       790        800        810        820        830        840 
TGFTGTVEVK NNGTAALNGW TLGFSFADGQ KVSQGWSAEW SQSGTAVTAK NAPWNGTLAA 

       850        860        870 
GSSVSIGFNG THNGTNTAPT AFTLNGVACT LG 

« Hide

References

« Hide 'large scale' references
[1]"Cellobiohydrolase A (CbhA) from the cellulolytic bacterium Cellulomonas fimi is a beta-1,4-exocellobiohydrolase analogous to Trichoderma reesei CBH II."
Meinke A., Gilkes N.R., Kwan E., Kilburn D.G., Warren R.A.J., Miller R.C. Jr.
Mol. Microbiol. 12:413-422(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
[2]"Complete sequence of Cellulomonas fimi ATCC 484."
US DOE Joint Genome Institute
Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Mead D., Brumm P., Woyke T.
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
[3]"Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase."
Meinke A., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
J. Bacteriol. 175:1910-1918(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 41-58.
Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L25809 Unassigned DNA. Translation: AAC36898.1.
CP002666 Genomic DNA. Translation: AEE46055.1.
PIRS49541.
RefSeqYP_004453442.1. NC_015514.1.

3D structure databases

ProteinModelPortalP50401.
SMRP50401. Positions 674-762, 765-872.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM2. Carbohydrate-Binding Module Family 2.
GH6. Glycoside Hydrolase Family 6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEE46055; AEE46055; Celf_1925.
GeneID10592708.
KEGGcfi:Celf_1925.
PATRIC54614786. VBICelFim18861_1929.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMANISEPAC.

Enzyme and pathway databases

BioCycCFIM590998:GJFK-1948-MONOMER.
SABIO-RKP50401.

Family and domain databases

Gene3D2.60.40.10. 3 hits.
2.60.40.290. 1 hit.
3.20.20.40. 1 hit.
InterProIPR016288. Beta_cellobiohydrolase.
IPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR018366. CBM2_CS.
IPR001919. Cellulose-bd_dom_fam2_bac.
IPR003961. Fibronectin_type3.
IPR001524. Glyco_hydro_6_CS.
IPR013783. Ig-like_fold.
[Graphical view]
PfamPF00553. CBM_2. 1 hit.
PF00041. fn3. 3 hits.
PF01341. Glyco_hydro_6. 1 hit.
[Graphical view]
PRINTSPR00733. GLHYDRLASE6.
SMARTSM00637. CBD_II. 1 hit.
SM00060. FN3. 3 hits.
[Graphical view]
SUPFAMSSF49265. SSF49265. 2 hits.
SSF49384. SSF49384. 1 hit.
SSF51989. SSF51989. 1 hit.
PROSITEPS51173. CBM2. 1 hit.
PS00561. CBM2_A. 1 hit.
PS50853. FN3. 3 hits.
PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUXA_CELFA
AccessionPrimary (citable) accession number: P50401
Secondary accession number(s): F4GZL1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: December 11, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries