Reviewed, UniProtKB/Swiss-Prot P50401 (GUXA_CELFI)

Names and origin

Protein names

Recommended name:
    Exoglucanase A
    EC=3.2.1.91
Alternative name(s):
    Exocellobiohydrolase A
    1,4-beta-cellobiohydrolase A
    CBP95

Gene names

Name:

cbhA

Organism

Cellulomonas fimi

Taxonomic identifier

1708 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Cellulomonadaceae › Cellulomonas

Protein attributes

Sequence length	872 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This enzyme hydrolyzes 1,4-beta-D-glucosidic linkages of cellulose. Weak activity against carboxymethylcellulose, bacterial microcrystalline cellulose and barley beta-glucan. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration.
Catalytic activity	Hydrolysis of 1,4-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.
Sequence similarities	Belongs to the glycosyl hydrolase 6 (cellulase B) family. Contains 1 CBM2 (carbohydrate binding type-2) domain. Contains 3 fibronectin type-III domains.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cellulose 1,4-beta-cellobiosidase activity Inferred from electronic annotation. Source: EC polysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 40	40	Ref.2
	Chain	41 – 872	832	Exoglucanase A		PRO_0000007904
Regions
	Domain	482 – 566	85	Fibronectin type-III 1
	Domain	577 – 664	88	Fibronectin type-III 2
	Domain	675 – 762	88	Fibronectin type-III 3
	Domain	763 – 872	110	CBM2
	Region	41 – 477	437	Catalytic
Sites
	Active site	188	1	Proton donor By similarity
	Active site	410	1	Nucleophile By similarity
Amino acid modifications
	Disulfide bond	140 ↔ 202		By similarity
	Disulfide bond	374 ↔ 428		By similarity
	Disulfide bond	770 ↔ 869		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50401-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 7883B407F995533B	FASTA	872	89,301
	10 20 30 40 50 60 MSTLGKRAGV RRRVRAVATA ATATALVAVP LTTLATSASA APVHVDNPYA GAVQYVNPTW 70 80 90 100 110 120 AASVNAAAGR QSADPALAAK MRTVAGQPTA VWMDRISAIT GNADGNGLKF HLDNAVAQQK 130 140 150 160 170 180 AAGVPLVFNL VIYDLPGRDC FALASNGELP ATDAGLARYK SEYIDPIADL LDNPEYESIR 190 200 210 220 230 240 IAATIEPDSL PNLTTNISEP ACQQAAPYYR QGVKYALDKL HAIPNVYNYI DIGHSGWLGW 250 260 270 280 290 300 DSNAGPSATL FAEVAKSTTA GFASIDGFVS DVANTTPLEE PLLSDSSLTI NNTPIRSSKF 310 320 330 340 350 360 YEWNFDFDEI DYTAHMHRLL VAAGFPSSIG MLVDTSRNGW GGPNRPTSIT ASTDVNAYVD 370 380 390 400 410 420 ANRVDRRVHR GAWCNPLGAG IGRFPEATPS GYAASHLDAF VWIKPPGESD GASTDIPNDQ 430 440 450 460 470 480 GKRFDRMCDP TFVSPKLNNQ LTGATPNAPL AGQWFEEQFV TLVKNAYPVI GGTTPVEDLV 490 500 510 520 530 540 APTVPTGLTA GTTTATSVPL SWTASTDNVA VTGYDVYRGT TLVGTTAATS YTVTGLTPAT 550 560 570 580 590 600 AYSFTVRAKD AAGNVSAASA AAAATTQSGT VTDTTAPSVP AGLTAGTTTT TTVPLSWTAS 610 620 630 640 650 660 TDNAGGSGVA GYEVLRGTTV VGTTTATSYT VTGLTAGTTY SFSVRAKDVA GNTSAASAAV 670 680 690 700 710 720 SATTQTGTVV DTTAPSVPTG LTAGTTTTSS VPLTWTASTD NAGGSGVAGY EVFNGTTRVA 730 740 750 760 770 780 TVTSTSYTVT GLAADTAYSF TVKAKDVAGN VSAASAAVSA RTQAATSGGC TVKYSASSWN 790 800 810 820 830 840 TGFTGTVEVK NNGTAALNGW TLGFSFADGQ KVSQGWSAEW SQSGTAVTAK NAPWNGTLAA 850 860 870 GSSVSIGFNG THNGTNTAPT AFTLNGVACT LG

References

[1]

"Cellobiohydrolase A (CbhA) from the cellulolytic bacterium Cellulomonas fimi is a beta-1,4-exocellobiohydrolase analogous to Trichoderma reesei CBH II." Meinke A., Gilkes N.R., Kwan E., Kilburn D.G., Warren R.A.J., Miller R.C. Jr. Mol. Microbiol. 12:413-422(1994) [PubMed: 8065260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 484 / DSM 20113 / IFO 15513 / JCM 1341 / NCTC 7547.

[2]

"Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase." Meinke A., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J. J. Bacteriol. 175:1910-1918(1993) [PubMed: 8458833] [Abstract] Cited for: PROTEIN SEQUENCE OF 41-58.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L25809 Unassigned DNA. Translation: AAC36898.1.
PIR	S49541.
3D structure databases
HSSP	HSSP built from PDB template 1K85 based on UniProtKB P20533.
ModBase	Search...
Protein family/group databases
CAZy	CBM2. Carbohydrate-Binding Module Family 2. GH6. Glycoside Hydrolase Family 6.
Enzyme and pathway databases
BRENDA	3.2.1.91. 97800.
Family and domain databases
InterPro	IPR016288. Beta_cellobiohydrolase. IPR001919. CBD_bac. IPR012291. CBD_carb_bd. IPR018366. CBM2_CS. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR001524. Glyco_hydro_6_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.290. CBD_carb_bd. 1 hit. G3DSA:2.60.40.30. FN_III-like. 3 hits. G3DSA:3.20.20.40. Glyco_hydro_6. 1 hit.
Pfam	PF00553. CBM_2. 1 hit. PF00041. fn3. 3 hits. PF01341. Glyco_hydro_6. 1 hit. [Graphical view]
PRINTS	PR00733. GLHYDRLASE6.
ProDom	PD003733. Glyco_hydro_6. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00637. CBD_II. 1 hit. SM00060. FN3. 3 hits. [Graphical view]
PROSITE	PS51173. CBM2. 1 hit. PS00561. CBM2_A. 1 hit. PS50853. FN3. 3 hits. PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit. PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUXA_CELFI

Accession

Primary (citable) accession number: P50401

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)