Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Exoglucanase A

Gene

cbhA

Organism
Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme hydrolyzes 1,4-beta-D-glucosidic linkages of cellulose. Weak activity against carboxymethylcellulose, bacterial microcrystalline cellulose and barley beta-glucan. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration.

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei188Proton donorPROSITE-ProRule annotation1
Active sitei410NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCFIM590998:G1GXC-1946-MONOMER
SABIO-RKiP50401

Protein family/group databases

CAZyiCBM2 Carbohydrate-Binding Module Family 2
GH6 Glycoside Hydrolase Family 6

Names & Taxonomyi

Protein namesi
Recommended name:
Exoglucanase A (EC:3.2.1.91)
Alternative name(s):
1,4-beta-cellobiohydrolase A
CBP95
Exocellobiohydrolase A
Gene namesi
Name:cbhA
Ordered Locus Names:Celf_1925
OrganismiCellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547)
Taxonomic identifieri590998 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesCellulomonadaceaeCellulomonas
Proteomesi
  • UP000008460 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 401 PublicationAdd BLAST40
ChainiPRO_000000790441 – 872Exoglucanase AAdd BLAST832

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi140 ↔ 202By similarity
Disulfide bondi374 ↔ 428By similarity
Disulfide bondi770 ↔ 869By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi590998.Celf_1925

Structurei

3D structure databases

ProteinModelPortaliP50401
SMRiP50401
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini484 – 569Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST86
Domaini579 – 667Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST89
Domaini677 – 765Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST89
Domaini763 – 872CBM2PROSITE-ProRule annotationAdd BLAST110

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni41 – 477CatalyticAdd BLAST437

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG41065HY Bacteria
COG5297 LUCA
KOiK19668
OMAiYEWNPNF
OrthoDBiPOG091H09LC

Family and domain databases

CDDicd00063 FN3, 2 hits
Gene3Di2.60.40.10, 3 hits
2.60.40.290, 1 hit
3.20.20.40, 1 hit
InterProiView protein in InterPro
IPR016288 Beta_cellobiohydrolase
IPR036434 Beta_cellobiohydrolase_sf
IPR001919 CBD2
IPR008965 CBM2/CBM3_carb-bd_dom_sf
IPR012291 CBM2_carb-bd_dom_sf
IPR018366 CBM2_CS
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR001524 Glyco_hydro_6_CS
IPR013783 Ig-like_fold
PANTHERiPTHR34876 PTHR34876, 1 hit
PfamiView protein in Pfam
PF00553 CBM_2, 1 hit
PF00041 fn3, 3 hits
PF01341 Glyco_hydro_6, 1 hit
PRINTSiPR00733 GLHYDRLASE6
SMARTiView protein in SMART
SM00637 CBD_II, 1 hit
SM00060 FN3, 3 hits
SUPFAMiSSF49265 SSF49265, 2 hits
SSF49384 SSF49384, 1 hit
SSF51989 SSF51989, 1 hit
PROSITEiView protein in PROSITE
PS51173 CBM2, 1 hit
PS00561 CBM2_A, 1 hit
PS50853 FN3, 3 hits
PS00655 GLYCOSYL_HYDROL_F6_1, 1 hit
PS00656 GLYCOSYL_HYDROL_F6_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50401-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTLGKRAGV RRRVRAVATA ATATALVAVP LTTLATSASA APVHVDNPYA
60 70 80 90 100
GAVQYVNPTW AASVNAAAGR QSADPALAAK MRTVAGQPTA VWMDRISAIT
110 120 130 140 150
GNADGNGLKF HLDNAVAQQK AAGVPLVFNL VIYDLPGRDC FALASNGELP
160 170 180 190 200
ATDAGLARYK SEYIDPIADL LDNPEYESIR IAATIEPDSL PNLTTNISEP
210 220 230 240 250
ACQQAAPYYR QGVKYALDKL HAIPNVYNYI DIGHSGWLGW DSNAGPSATL
260 270 280 290 300
FAEVAKSTTA GFASIDGFVS DVANTTPLEE PLLSDSSLTI NNTPIRSSKF
310 320 330 340 350
YEWNFDFDEI DYTAHMHRLL VAAGFPSSIG MLVDTSRNGW GGPNRPTSIT
360 370 380 390 400
ASTDVNAYVD ANRVDRRVHR GAWCNPLGAG IGRFPEATPS GYAASHLDAF
410 420 430 440 450
VWIKPPGESD GASTDIPNDQ GKRFDRMCDP TFVSPKLNNQ LTGATPNAPL
460 470 480 490 500
AGQWFEEQFV TLVKNAYPVI GGTTPVEDLV APTVPTGLTA GTTTATSVPL
510 520 530 540 550
SWTASTDNVA VTGYDVYRGT TLVGTTAATS YTVTGLTPAT AYSFTVRAKD
560 570 580 590 600
AAGNVSAASA AAAATTQSGT VTDTTAPSVP AGLTAGTTTT TTVPLSWTAS
610 620 630 640 650
TDNAGGSGVA GYEVLRGTTV VGTTTATSYT VTGLTAGTTY SFSVRAKDVA
660 670 680 690 700
GNTSAASAAV SATTQTGTVV DTTAPSVPTG LTAGTTTTSS VPLTWTASTD
710 720 730 740 750
NAGGSGVAGY EVFNGTTRVA TVTSTSYTVT GLAADTAYSF TVKAKDVAGN
760 770 780 790 800
VSAASAAVSA RTQAATSGGC TVKYSASSWN TGFTGTVEVK NNGTAALNGW
810 820 830 840 850
TLGFSFADGQ KVSQGWSAEW SQSGTAVTAK NAPWNGTLAA GSSVSIGFNG
860 870
THNGTNTAPT AFTLNGVACT LG
Length:872
Mass (Da):89,301
Last modified:October 1, 1996 - v1
Checksum:i7883B407F995533B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25809 Unassigned DNA Translation: AAC36898.1
CP002666 Genomic DNA Translation: AEE46055.1
PIRiS49541
RefSeqiWP_013771081.1, NC_015514.1

Genome annotation databases

EnsemblBacteriaiAEE46055; AEE46055; Celf_1925
KEGGicfi:Celf_1925

Similar proteinsi

Entry informationi

Entry nameiGUXA_CELFA
AccessioniPrimary (citable) accession number: P50401
Secondary accession number(s): F4GZL1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health