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Reviewed, UniProtKB/Swiss-Prot P50401 (GUXA_CELFI)

Last modified June 16, 2009. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Exoglucanase A
    EC=3.2.1.91
Alternative name(s):
    Exocellobiohydrolase A
    1,4-beta-cellobiohydrolase A
    CBP95
Gene names
Name: cbhA
OrganismCellulomonas fimi
Taxonomic identifier1708 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeCellulomonadaceaeCellulomonas

Protein attributes

Sequence length872 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

This enzyme hydrolyzes 1,4-beta-D-glucosidic linkages of cellulose. Weak activity against carboxymethylcellulose, bacterial microcrystalline cellulose and barley beta-glucan. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration.

Catalytic activity

Hydrolysis of 1,4-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sequence similarities

Belongs to the glycosyl hydrolase 6 (cellulase B) family.

Contains 1 CBM2 (carbohydrate binding type-2) domain.

Contains 3 fibronectin type-III domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4040 Ref.2
Chain41 – 872832Exoglucanase A
PRO_0000007904

Regions

Domain482 – 56685Fibronectin type-III 1
Domain577 – 66488Fibronectin type-III 2
Domain675 – 76288Fibronectin type-III 3
Domain763 – 872110CBM2
Region41 – 477437Catalytic

Sites

Active site1881Proton donor By similarity
Active site4101Nucleophile By similarity

Amino acid modifications

Disulfide bond140 ↔ 202 By similarity
Disulfide bond374 ↔ 428 By similarity
Disulfide bond770 ↔ 869 By similarity

Sequences

Sequence LengthMass (Da)Tools
P50401-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 7883B407F995533B

FASTA87289,301
        10         20         30         40         50         60 
MSTLGKRAGV RRRVRAVATA ATATALVAVP LTTLATSASA APVHVDNPYA GAVQYVNPTW 

        70         80         90        100        110        120 
AASVNAAAGR QSADPALAAK MRTVAGQPTA VWMDRISAIT GNADGNGLKF HLDNAVAQQK 

       130        140        150        160        170        180 
AAGVPLVFNL VIYDLPGRDC FALASNGELP ATDAGLARYK SEYIDPIADL LDNPEYESIR 

       190        200        210        220        230        240 
IAATIEPDSL PNLTTNISEP ACQQAAPYYR QGVKYALDKL HAIPNVYNYI DIGHSGWLGW 

       250        260        270        280        290        300 
DSNAGPSATL FAEVAKSTTA GFASIDGFVS DVANTTPLEE PLLSDSSLTI NNTPIRSSKF 

       310        320        330        340        350        360 
YEWNFDFDEI DYTAHMHRLL VAAGFPSSIG MLVDTSRNGW GGPNRPTSIT ASTDVNAYVD 

       370        380        390        400        410        420 
ANRVDRRVHR GAWCNPLGAG IGRFPEATPS GYAASHLDAF VWIKPPGESD GASTDIPNDQ 

       430        440        450        460        470        480 
GKRFDRMCDP TFVSPKLNNQ LTGATPNAPL AGQWFEEQFV TLVKNAYPVI GGTTPVEDLV 

       490        500        510        520        530        540 
APTVPTGLTA GTTTATSVPL SWTASTDNVA VTGYDVYRGT TLVGTTAATS YTVTGLTPAT 

       550        560        570        580        590        600 
AYSFTVRAKD AAGNVSAASA AAAATTQSGT VTDTTAPSVP AGLTAGTTTT TTVPLSWTAS 

       610        620        630        640        650        660 
TDNAGGSGVA GYEVLRGTTV VGTTTATSYT VTGLTAGTTY SFSVRAKDVA GNTSAASAAV 

       670        680        690        700        710        720 
SATTQTGTVV DTTAPSVPTG LTAGTTTTSS VPLTWTASTD NAGGSGVAGY EVFNGTTRVA 

       730        740        750        760        770        780 
TVTSTSYTVT GLAADTAYSF TVKAKDVAGN VSAASAAVSA RTQAATSGGC TVKYSASSWN 

       790        800        810        820        830        840 
TGFTGTVEVK NNGTAALNGW TLGFSFADGQ KVSQGWSAEW SQSGTAVTAK NAPWNGTLAA 

       850        860        870 
GSSVSIGFNG THNGTNTAPT AFTLNGVACT LG 

« Hide

References

[1]"Cellobiohydrolase A (CbhA) from the cellulolytic bacterium Cellulomonas fimi is a beta-1,4-exocellobiohydrolase analogous to Trichoderma reesei CBH II."
Meinke A., Gilkes N.R., Kwan E., Kilburn D.G., Warren R.A.J., Miller R.C. Jr.
Mol. Microbiol. 12:413-422(1994) [PubMed: 8065260] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 484 / DSM 20113 / IFO 15513 / JCM 1341 / NCTC 7547.
[2]"Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase."
Meinke A., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
J. Bacteriol. 175:1910-1918(1993) [PubMed: 8458833] [Abstract]
Cited for: PROTEIN SEQUENCE OF 41-58.

Cross-references

Sequence databases

L25809 Unassigned DNA. Translation: AAC36898.1.
PIRS49541.

3D structure databases

HSSPHSSP built from PDB template 1K85 based on UniProtKB P20533.
ModBaseSearch...

Protein family/group databases

CAZyCBM2. Carbohydrate-Binding Module Family 2.
GH6. Glycoside Hydrolase Family 6.

Enzyme and pathway databases

BRENDA3.2.1.91. 97800.

Family and domain databases

InterProIPR016288. Beta_cellobiohydrolase.
IPR001919. CBD_bac.
IPR012291. CBD_carb_bd.
IPR018366. CBM2_CS.
IPR008957. Fibronectin_typ-III-like_fold.
IPR003961. FN_III.
IPR001524. Glyco_hydro_6_CS.
[Graphical view]
Gene3DG3DSA:2.60.40.290. CBD_carb_bd. 1 hit.
G3DSA:2.60.40.30. FN_III-like. 3 hits.
G3DSA:3.20.20.40. Glyco_hydro_6. 1 hit.
PfamPF00553. CBM_2. 1 hit.
PF00041. fn3. 3 hits.
PF01341. Glyco_hydro_6. 1 hit.
[Graphical view]
PRINTSPR00733. GLHYDRLASE6.
ProDomPD003733. Glyco_hydro_6. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00637. CBD_II. 1 hit.
SM00060. FN3. 3 hits.
[Graphical view]
PROSITEPS51173. CBM2. 1 hit.
PS00561. CBM2_A. 1 hit.
PS50853. FN3. 3 hits.
PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUXA_CELFI
AccessionPrimary (citable) accession number: P50401
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents