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P50401

- GUXA_CELFA

UniProt

P50401 - GUXA_CELFA

Protein

Exoglucanase A

Gene

cbhA

Organism
Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    This enzyme hydrolyzes 1,4-beta-D-glucosidic linkages of cellulose. Weak activity against carboxymethylcellulose, bacterial microcrystalline cellulose and barley beta-glucan. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration.

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei188 – 1881Proton donorPROSITE-ProRule annotation
    Active sitei410 – 4101NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB-EC
    2. polysaccharide binding Source: InterPro

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciCFIM590998:GJFK-1948-MONOMER.
    SABIO-RKP50401.

    Protein family/group databases

    CAZyiCBM2. Carbohydrate-Binding Module Family 2.
    GH6. Glycoside Hydrolase Family 6.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exoglucanase A (EC:3.2.1.91)
    Alternative name(s):
    1,4-beta-cellobiohydrolase A
    CBP95
    Exocellobiohydrolase A
    Gene namesi
    Name:cbhA
    Ordered Locus Names:Celf_1925
    OrganismiCellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547)
    Taxonomic identifieri590998 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeCellulomonadaceaeCellulomonas
    ProteomesiUP000008460: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 40401 PublicationAdd
    BLAST
    Chaini41 – 872832Exoglucanase APRO_0000007904Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi140 ↔ 202By similarity
    Disulfide bondi374 ↔ 428By similarity
    Disulfide bondi770 ↔ 869By similarity

    Keywords - PTMi

    Disulfide bond

    Structurei

    3D structure databases

    ProteinModelPortaliP50401.
    SMRiP50401. Positions 674-762, 765-872.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini484 – 56986Fibronectin type-III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini579 – 66789Fibronectin type-III 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini677 – 76589Fibronectin type-III 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini763 – 872110CBM2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni41 – 477437CatalyticAdd
    BLAST

    Sequence similaritiesi

    Contains 3 fibronectin type-III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    OMAiAYSFTVR.

    Family and domain databases

    Gene3Di2.60.40.10. 3 hits.
    2.60.40.290. 1 hit.
    3.20.20.40. 1 hit.
    InterProiIPR016288. Beta_cellobiohydrolase.
    IPR008965. Carb-bd_dom.
    IPR012291. CBD_carb-bd_dom.
    IPR018366. CBM2_CS.
    IPR001919. Cellulose-bd_dom_fam2_bac.
    IPR003961. Fibronectin_type3.
    IPR001524. Glyco_hydro_6_CS.
    IPR013783. Ig-like_fold.
    [Graphical view]
    PfamiPF00553. CBM_2. 1 hit.
    PF00041. fn3. 3 hits.
    PF01341. Glyco_hydro_6. 1 hit.
    [Graphical view]
    PRINTSiPR00733. GLHYDRLASE6.
    SMARTiSM00637. CBD_II. 1 hit.
    SM00060. FN3. 3 hits.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 2 hits.
    SSF49384. SSF49384. 1 hit.
    SSF51989. SSF51989. 1 hit.
    PROSITEiPS51173. CBM2. 1 hit.
    PS00561. CBM2_A. 1 hit.
    PS50853. FN3. 3 hits.
    PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
    PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50401-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTLGKRAGV RRRVRAVATA ATATALVAVP LTTLATSASA APVHVDNPYA    50
    GAVQYVNPTW AASVNAAAGR QSADPALAAK MRTVAGQPTA VWMDRISAIT 100
    GNADGNGLKF HLDNAVAQQK AAGVPLVFNL VIYDLPGRDC FALASNGELP 150
    ATDAGLARYK SEYIDPIADL LDNPEYESIR IAATIEPDSL PNLTTNISEP 200
    ACQQAAPYYR QGVKYALDKL HAIPNVYNYI DIGHSGWLGW DSNAGPSATL 250
    FAEVAKSTTA GFASIDGFVS DVANTTPLEE PLLSDSSLTI NNTPIRSSKF 300
    YEWNFDFDEI DYTAHMHRLL VAAGFPSSIG MLVDTSRNGW GGPNRPTSIT 350
    ASTDVNAYVD ANRVDRRVHR GAWCNPLGAG IGRFPEATPS GYAASHLDAF 400
    VWIKPPGESD GASTDIPNDQ GKRFDRMCDP TFVSPKLNNQ LTGATPNAPL 450
    AGQWFEEQFV TLVKNAYPVI GGTTPVEDLV APTVPTGLTA GTTTATSVPL 500
    SWTASTDNVA VTGYDVYRGT TLVGTTAATS YTVTGLTPAT AYSFTVRAKD 550
    AAGNVSAASA AAAATTQSGT VTDTTAPSVP AGLTAGTTTT TTVPLSWTAS 600
    TDNAGGSGVA GYEVLRGTTV VGTTTATSYT VTGLTAGTTY SFSVRAKDVA 650
    GNTSAASAAV SATTQTGTVV DTTAPSVPTG LTAGTTTTSS VPLTWTASTD 700
    NAGGSGVAGY EVFNGTTRVA TVTSTSYTVT GLAADTAYSF TVKAKDVAGN 750
    VSAASAAVSA RTQAATSGGC TVKYSASSWN TGFTGTVEVK NNGTAALNGW 800
    TLGFSFADGQ KVSQGWSAEW SQSGTAVTAK NAPWNGTLAA GSSVSIGFNG 850
    THNGTNTAPT AFTLNGVACT LG 872
    Length:872
    Mass (Da):89,301
    Last modified:October 1, 1996 - v1
    Checksum:i7883B407F995533B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L25809 Unassigned DNA. Translation: AAC36898.1.
    CP002666 Genomic DNA. Translation: AEE46055.1.
    PIRiS49541.
    RefSeqiWP_013771081.1. NC_015514.1.
    YP_004453442.1. NC_015514.1.

    Genome annotation databases

    EnsemblBacteriaiAEE46055; AEE46055; Celf_1925.
    GeneIDi10592708.
    KEGGicfi:Celf_1925.
    PATRICi54614786. VBICelFim18861_1929.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L25809 Unassigned DNA. Translation: AAC36898.1 .
    CP002666 Genomic DNA. Translation: AEE46055.1 .
    PIRi S49541.
    RefSeqi WP_013771081.1. NC_015514.1.
    YP_004453442.1. NC_015514.1.

    3D structure databases

    ProteinModelPortali P50401.
    SMRi P50401. Positions 674-762, 765-872.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM2. Carbohydrate-Binding Module Family 2.
    GH6. Glycoside Hydrolase Family 6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AEE46055 ; AEE46055 ; Celf_1925 .
    GeneIDi 10592708.
    KEGGi cfi:Celf_1925.
    PATRICi 54614786. VBICelFim18861_1929.

    Phylogenomic databases

    OMAi AYSFTVR.

    Enzyme and pathway databases

    BioCyci CFIM590998:GJFK-1948-MONOMER.
    SABIO-RK P50401.

    Family and domain databases

    Gene3Di 2.60.40.10. 3 hits.
    2.60.40.290. 1 hit.
    3.20.20.40. 1 hit.
    InterProi IPR016288. Beta_cellobiohydrolase.
    IPR008965. Carb-bd_dom.
    IPR012291. CBD_carb-bd_dom.
    IPR018366. CBM2_CS.
    IPR001919. Cellulose-bd_dom_fam2_bac.
    IPR003961. Fibronectin_type3.
    IPR001524. Glyco_hydro_6_CS.
    IPR013783. Ig-like_fold.
    [Graphical view ]
    Pfami PF00553. CBM_2. 1 hit.
    PF00041. fn3. 3 hits.
    PF01341. Glyco_hydro_6. 1 hit.
    [Graphical view ]
    PRINTSi PR00733. GLHYDRLASE6.
    SMARTi SM00637. CBD_II. 1 hit.
    SM00060. FN3. 3 hits.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 2 hits.
    SSF49384. SSF49384. 1 hit.
    SSF51989. SSF51989. 1 hit.
    PROSITEi PS51173. CBM2. 1 hit.
    PS00561. CBM2_A. 1 hit.
    PS50853. FN3. 3 hits.
    PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
    PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cellobiohydrolase A (CbhA) from the cellulolytic bacterium Cellulomonas fimi is a beta-1,4-exocellobiohydrolase analogous to Trichoderma reesei CBH II."
      Meinke A., Gilkes N.R., Kwan E., Kilburn D.G., Warren R.A.J., Miller R.C. Jr.
      Mol. Microbiol. 12:413-422(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
    2. "Complete sequence of Cellulomonas fimi ATCC 484."
      US DOE Joint Genome Institute
      Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Mead D., Brumm P., Woyke T.
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
    3. "Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase."
      Meinke A., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
      J. Bacteriol. 175:1910-1918(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 41-58.
      Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.

    Entry informationi

    Entry nameiGUXA_CELFA
    AccessioniPrimary (citable) accession number: P50401
    Secondary accession number(s): F4GZL1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3