P50401 (GUXA_CELFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exoglucanase A
EC=3.2.1.91

Alternative name(s):
1,4-beta-cellobiohydrolase A
CBP95
Exocellobiohydrolase A

Gene names

Name:	cbhA
Ordered Locus Names:	Celf_1925

Organism

Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) [Complete proteome] [HAMAP]

Taxonomic identifier

590998 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Cellulomonadaceae › Cellulomonas › Cellulomonas fimi

Protein attributes

Sequence length	872 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme hydrolyzes 1,4-beta-D-glucosidic linkages of cellulose. Weak activity against carboxymethylcellulose, bacterial microcrystalline cellulose and barley beta-glucan. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration.
Catalytic activity	Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.
Sequence similarities	Belongs to the glycosyl hydrolase 6 (cellulase B) family. Contains 1 CBM2 (carbohydrate binding type-2) domain. Contains 3 fibronectin type-III domains.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulose 1,4-beta-cellobiosidase activity Inferred from electronic annotation. Source: EC polysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 40	40	Ref.3
	Chain	41 – 872	832	Exoglucanase A		PRO_0000007904
Regions
	Domain	482 – 566	85	Fibronectin type-III 1
	Domain	577 – 664	88	Fibronectin type-III 2
	Domain	675 – 762	88	Fibronectin type-III 3
	Domain	763 – 872	110	CBM2
	Region	41 – 477	437	Catalytic
Sites
	Active site	188	1	Proton donor By similarity
	Active site	410	1	Nucleophile By similarity
Amino acid modifications
	Disulfide bond	140 ↔ 202		By similarity
	Disulfide bond	374 ↔ 428		By similarity
	Disulfide bond	770 ↔ 869		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50401 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 7883B407F995533B	FASTA	872	89,301
	10 20 30 40 50 60 MSTLGKRAGV RRRVRAVATA ATATALVAVP LTTLATSASA APVHVDNPYA GAVQYVNPTW 70 80 90 100 110 120 AASVNAAAGR QSADPALAAK MRTVAGQPTA VWMDRISAIT GNADGNGLKF HLDNAVAQQK 130 140 150 160 170 180 AAGVPLVFNL VIYDLPGRDC FALASNGELP ATDAGLARYK SEYIDPIADL LDNPEYESIR 190 200 210 220 230 240 IAATIEPDSL PNLTTNISEP ACQQAAPYYR QGVKYALDKL HAIPNVYNYI DIGHSGWLGW 250 260 270 280 290 300 DSNAGPSATL FAEVAKSTTA GFASIDGFVS DVANTTPLEE PLLSDSSLTI NNTPIRSSKF 310 320 330 340 350 360 YEWNFDFDEI DYTAHMHRLL VAAGFPSSIG MLVDTSRNGW GGPNRPTSIT ASTDVNAYVD 370 380 390 400 410 420 ANRVDRRVHR GAWCNPLGAG IGRFPEATPS GYAASHLDAF VWIKPPGESD GASTDIPNDQ 430 440 450 460 470 480 GKRFDRMCDP TFVSPKLNNQ LTGATPNAPL AGQWFEEQFV TLVKNAYPVI GGTTPVEDLV 490 500 510 520 530 540 APTVPTGLTA GTTTATSVPL SWTASTDNVA VTGYDVYRGT TLVGTTAATS YTVTGLTPAT 550 560 570 580 590 600 AYSFTVRAKD AAGNVSAASA AAAATTQSGT VTDTTAPSVP AGLTAGTTTT TTVPLSWTAS 610 620 630 640 650 660 TDNAGGSGVA GYEVLRGTTV VGTTTATSYT VTGLTAGTTY SFSVRAKDVA GNTSAASAAV 670 680 690 700 710 720 SATTQTGTVV DTTAPSVPTG LTAGTTTTSS VPLTWTASTD NAGGSGVAGY EVFNGTTRVA 730 740 750 760 770 780 TVTSTSYTVT GLAADTAYSF TVKAKDVAGN VSAASAAVSA RTQAATSGGC TVKYSASSWN 790 800 810 820 830 840 TGFTGTVEVK NNGTAALNGW TLGFSFADGQ KVSQGWSAEW SQSGTAVTAK NAPWNGTLAA 850 860 870 GSSVSIGFNG THNGTNTAPT AFTLNGVACT LG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cellobiohydrolase A (CbhA) from the cellulolytic bacterium Cellulomonas fimi is a beta-1,4-exocellobiohydrolase analogous to Trichoderma reesei CBH II." Meinke A., Gilkes N.R., Kwan E., Kilburn D.G., Warren R.A.J., Miller R.C. Jr. Mol. Microbiol. 12:413-422(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
[2]	"Complete sequence of Cellulomonas fimi ATCC 484." US DOE Joint Genome Institute Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Mead D., Brumm P., Woyke T. Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
[3]	"Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase." Meinke A., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J. J. Bacteriol. 175:1910-1918(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 41-58. Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L25809 Unassigned DNA. Translation: AAC36898.1. CP002666 Genomic DNA. Translation: AEE46055.1.
PIR	S49541.
RefSeq	YP_004453442.1. NC_015514.1.
3D structure databases
ProteinModelPortal	P50401.
SMR	P50401. Positions 674-762, 765-872.
ModBase	Search...
Protein family/group databases
CAZy	CBM2. Carbohydrate-Binding Module Family 2. GH6. Glycoside Hydrolase Family 6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEE46055; AEE46055; Celf_1925.
GeneID	10592708.
KEGG	cfi:Celf_1925.
PATRIC	54614786. VBICelFim18861_1929.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BioCyc	CFIM590998:GJFK-1948-MONOMER.
Family and domain databases
Gene3D	2.60.40.10. 3 hits. 2.60.40.290. 1 hit. 3.20.20.40. 1 hit.
InterPro	IPR016288. Beta_cellobiohydrolase. IPR008965. Carb-bd_dom. IPR012291. CBD_carb-bd_dom. IPR018366. CBM2_CS. IPR001919. Cellulose-bd_dom_fam2_bac. IPR003961. Fibronectin_type3. IPR001524. Glyco_hydro_6_CS. IPR013783. Ig-like_fold. [Graphical view]
Pfam	PF00553. CBM_2. 1 hit. PF00041. fn3. 3 hits. PF01341. Glyco_hydro_6. 1 hit. [Graphical view]
PRINTS	PR00733. GLHYDRLASE6.
SMART	SM00637. CBD_II. 1 hit. SM00060. FN3. 3 hits. [Graphical view]
SUPFAM	SSF49384. Cellul_bind. 1 hit. SSF49265. FN_III-like. 3 hits. SSF51989. Glyco_hydro_6. 1 hit.
PROSITE	PS51173. CBM2. 1 hit. PS00561. CBM2_A. 1 hit. PS50853. FN3. 3 hits. PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit. PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUXA_CELFA

Accession

Primary (citable) accession number: P50401
Secondary accession number(s): F4GZL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program