Reviewed, UniProtKB/Swiss-Prot P50400 (GUND_CELFI)

Names and origin

Protein names

Recommended name:
    Endoglucanase D
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta-glucanase D
    Cellulase D

Gene names

Name:

cenD

Organism

Cellulomonas fimi

Taxonomic identifier

1708 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Cellulomonadaceae › Cellulomonas

Protein attributes

Sequence length	747 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Pathway	Glycan metabolism; cellulose degradation.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family. Contains 1 CBM2 (carbohydrate binding type-2) domain. Contains 2 fibronectin type-III domains.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC polysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 39	39	Potential
	Chain	40 – 747	708	Endoglucanase D		PRO_0000007843
Regions
	Domain	454 – 540	87	Fibronectin type-III 1
	Domain	550 – 635	86	Fibronectin type-III 2
	Domain	638 – 747	110	CBM2
Sites
	Active site	208	1	Proton donor By similarity
	Active site	349	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50400-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: BD15473C9D8B42BD	FASTA	747	78,937
	10 20 30 40 50 60 MHSASRTRAR TRVRTAVSGL LAATVLAAPL TLVAAPAQAA TGDDWLHVEG NTIVDSTGKE 70 80 90 100 110 120 AILSGVNWFG FNASERVFHG LWSGNITQIT QQMAQRGINV VRVPVSTQLL LEWKAGTFLK 130 140 150 160 170 180 PNVNTYANPE LEGKNSLQIF EYWLTLCQKY GIKVFLDVHS AEADNSGHVY NMWWKGDITT 190 200 210 220 230 240 EDVYEGWEWA ATRWKDDDTI VGADIKNEPH GTQGSTERAK WDGTTDKDNF KHFAETASKK 250 260 270 280 290 300 ILAINPNWLV FVEGVEIYPK PGVPWTSTGL TDYYGTWWGG NLRGVRDHPI DLGAHQDQLV 310 320 330 340 350 360 YSPHDYGPLV FDQKWFQKDF DKASLTADVW GPNWLFIHDE DIAPLLIGEW GGRLGQDPRQ 370 380 390 400 410 420 DKWMAALRDL VAERRLSQTF WVLNPNSGDT GGLLLDDWKT WDEVKYSTML EPTLWKHGGK 430 440 450 460 470 480 YVGLDHQVPL GGVGSTTGTS ISQVGGGTPD TTAPTAPTGL RAGTPTASTV PLTWSASTDT 490 500 510 520 530 540 GGSGVAGYEV YRGTTLVGTT TATSYTVTGL AADSAYTFSV RAKDGAGNTS AASAAVTART 550 560 570 580 590 600 AAGGGDVTAP SVPTGLTAGT PTATSVPLTW TASTDTGGSG VTGYEVYRGS TLVARPTGTS 610 620 630 640 650 660 HTVTGLSAAT AYTFTVRAVD AAGNVSAASA PVGVTTAPDP TTGSCAVTYT ANGWSGGFTA 670 680 690 700 710 720 AVTLTNTGTT ALSGWTLGFA FPSGQTLTQG WSARWAQSGS SVTATNEAWN AVLAPGASVE 730 740 IGFSGTHTGT NTAPATFTVG GATCTTR

References

[1]	"Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase." Meinke A., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J. J. Bacteriol. 175:1910-1918(1993) [PubMed: 8458833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L02544 Genomic DNA. Translation: AAA23089.1.
PIR	B47093.
3D structure databases
HSSP	HSSP built from PDB template 1K85 based on UniProtKB P20533.
ModBase	Search...
Protein family/group databases
CAZy	CBM2. Carbohydrate-Binding Module Family 2. GH5. Glycoside Hydrolase Family 5.
Enzyme and pathway databases
BRENDA	3.2.1.4. 97800.
Family and domain databases
InterPro	IPR001919. CBD_bac. IPR012291. CBD_carb_bd. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.290. CBD_carb_bd. 1 hit. G3DSA:2.60.40.30. FN_III-like. 2 hits. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00553. CBM_2. 1 hit. PF00150. Cellulase. 1 hit. PF00041. fn3. 2 hits. [Graphical view]
SMART	SM00637. CBD_II. 1 hit. SM00060. FN3. 2 hits. [Graphical view]
PROSITE	PS51173. CBM2. 1 hit. PS50853. FN3. 2 hits. PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUND_CELFI

Accession

Primary (citable) accession number: P50400

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)