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P50396

- GDIA_MOUSE

UniProt

P50396 - GDIA_MOUSE

Protein

Rab GDP dissociation inhibitor alpha

Gene

Gdi1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 3 (10 May 2004)
      Previous versions | rss
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    Functioni

    Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Promotes the dissociation of GDP-bound Rab proteins from the membrane and inhibits their activation. Promotes the dissociation of RAB1A, RAB3A, RAB5A and RAB10 from membranes.

    GO - Molecular functioni

    1. GTPase activator activity Source: UniProtKB-KW
    2. Rab GDP-dissociation inhibitor activity Source: UniProtKB

    GO - Biological processi

    1. negative regulation of axonogenesis Source: UniProtKB
    2. negative regulation of protein targeting to membrane Source: UniProtKB
    3. protein transport Source: InterPro
    4. Rab protein signal transduction Source: UniProtKB
    5. regulation of catalytic activity Source: GOC
    6. response to calcium ion Source: Ensembl

    Keywords - Molecular functioni

    GTPase activation

    Enzyme and pathway databases

    ReactomeiREACT_210090. Rho GTPase cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rab GDP dissociation inhibitor alpha
    Short name:
    Rab GDI alpha
    Alternative name(s):
    Guanosine diphosphate dissociation inhibitor 1
    Short name:
    GDI-1
    Gene namesi
    Name:Gdi1
    Synonyms:Rabgdia
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:99846. Gdi1.

    Subcellular locationi

    Cytoplasm By similarity. Golgi apparatustrans-Golgi network By similarity

    GO - Cellular componenti

    1. Golgi apparatus Source: UniProtKB-SubCell
    2. midbody Source: Ensembl
    3. neuron projection Source: Ensembl
    4. protein complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 447447Rab GDP dissociation inhibitor alphaPRO_0000056673Add
    BLAST

    Proteomic databases

    MaxQBiP50396.
    PaxDbiP50396.
    PRIDEiP50396.

    PTM databases

    PhosphoSiteiP50396.

    Expressioni

    Tissue specificityi

    High expression in brain, lower in other tissues.

    Gene expression databases

    ArrayExpressiP50396.
    BgeeiP50396.
    GenevestigatoriP50396.

    Interactioni

    Subunit structurei

    Interacts with RHOH, RAB1A, RAB3A and RAB5A By similarity. Interacts with RAB10.By similarity1 Publication

    Protein-protein interaction databases

    IntActiP50396. 7 interactions.
    MINTiMINT-1869124.

    Structurei

    3D structure databases

    ProteinModelPortaliP50396.
    SMRiP50396. Positions 1-430.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Rab GDI family.Curated

    Phylogenomic databases

    eggNOGiCOG5044.
    GeneTreeiENSGT00530000063044.
    HOVERGENiHBG000839.
    InParanoidiP50396.
    KOiK17255.
    OMAiKTWKDFD.
    OrthoDBiEOG7PP56D.
    PhylomeDBiP50396.
    TreeFamiTF300449.

    Family and domain databases

    Gene3Di3.40.50.720. 2 hits.
    InterProiIPR018203. GDP_dissociation_inhibitor.
    IPR016040. NAD(P)-bd_dom.
    IPR000806. RabGDI.
    [Graphical view]
    PfamiPF00996. GDI. 1 hit.
    [Graphical view]
    PRINTSiPR00892. RABGDI.
    PR00891. RABGDIREP.

    Sequencei

    Sequence statusi: Complete.

    P50396-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDEEYDVIVL GTGLTECILS GIMSVNGKKV LHMDRNPYYG GESSSITPLE    50
    ELYKRFQILE GPPESMGRGR DWNVDLIPKF LMANGQLVKM LLYTEVTRYL 100
    DFKVVEGSFV YKGGKIYKVP STETEALASN LMGMFEKRRF RKFLVFVANF 150
    DENDPKTFEG VDPQNTSMRD VYRKFDLGQD VIDFTGHALA LYRTDDYLDQ 200
    PCLETINRIK LYSESLARYG KSPYLYPLYG LGELPQGFAR LSAIYGGTYM 250
    LNKPVDDIIM ENGKVVGVKS EGEVARCKQL ICDPSYIPDR VQKAGQVIRI 300
    ICILSHPIKN TNDANSCQII IPQNQVNRKS DIYVCMISYA HNVAAQGKYI 350
    AIASTTVETA EPEKEVEPAL ELLEPIDQKF VAISDLYEPI DDGSESQVFC 400
    SCSYDATTHF ETTCNDIKDI YKRMAGSAFD FENMKRKQND VFGEADQ 447
    Length:447
    Mass (Da):50,522
    Last modified:May 10, 2004 - v3
    Checksum:iF7B9BC942B35DF58
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti58 – 581I → M in AAK49815. (PubMed:11027356)Curated
    Sequence conflicti58 – 581I → M in BAE33632. (PubMed:16141072)Curated
    Sequence conflicti58 – 581I → M in BAE42320. (PubMed:16141072)Curated
    Sequence conflicti128 – 1281A → R in AAB16907. (PubMed:7513052)Curated
    Sequence conflicti165 – 1651N → T in AAK49815. (PubMed:11027356)Curated
    Sequence conflicti196 – 1961D → E in AAB16907. (PubMed:7513052)Curated
    Sequence conflicti223 – 2231P → S in AAL60197. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF251042 mRNA. Translation: AAK49815.1.
    AF441240 Genomic DNA. Translation: AAL60197.1.
    AK028880 mRNA. Translation: BAC26169.1.
    AK156224 mRNA. Translation: BAE33632.1.
    AK171216 mRNA. Translation: BAE42320.1.
    AL807376 Genomic DNA. Translation: CAM24345.1.
    BC010220 mRNA. Translation: AAH10220.1.
    BC013758 mRNA. Translation: AAH13758.1.
    BC037598 mRNA. Translation: AAH37598.1.
    U07950 mRNA. Translation: AAB16907.1.
    CCDSiCCDS30227.1.
    PIRiC56024.
    RefSeqiNP_034403.1. NM_010273.4.
    UniGeneiMm.205830.

    Genome annotation databases

    EnsembliENSMUST00000015435; ENSMUSP00000015435; ENSMUSG00000015291.
    GeneIDi14567.
    KEGGimmu:14567.
    UCSCiuc009tom.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF251042 mRNA. Translation: AAK49815.1 .
    AF441240 Genomic DNA. Translation: AAL60197.1 .
    AK028880 mRNA. Translation: BAC26169.1 .
    AK156224 mRNA. Translation: BAE33632.1 .
    AK171216 mRNA. Translation: BAE42320.1 .
    AL807376 Genomic DNA. Translation: CAM24345.1 .
    BC010220 mRNA. Translation: AAH10220.1 .
    BC013758 mRNA. Translation: AAH13758.1 .
    BC037598 mRNA. Translation: AAH37598.1 .
    U07950 mRNA. Translation: AAB16907.1 .
    CCDSi CCDS30227.1.
    PIRi C56024.
    RefSeqi NP_034403.1. NM_010273.4.
    UniGenei Mm.205830.

    3D structure databases

    ProteinModelPortali P50396.
    SMRi P50396. Positions 1-430.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P50396. 7 interactions.
    MINTi MINT-1869124.

    PTM databases

    PhosphoSitei P50396.

    Proteomic databases

    MaxQBi P50396.
    PaxDbi P50396.
    PRIDEi P50396.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000015435 ; ENSMUSP00000015435 ; ENSMUSG00000015291 .
    GeneIDi 14567.
    KEGGi mmu:14567.
    UCSCi uc009tom.2. mouse.

    Organism-specific databases

    CTDi 2664.
    MGIi MGI:99846. Gdi1.

    Phylogenomic databases

    eggNOGi COG5044.
    GeneTreei ENSGT00530000063044.
    HOVERGENi HBG000839.
    InParanoidi P50396.
    KOi K17255.
    OMAi KTWKDFD.
    OrthoDBi EOG7PP56D.
    PhylomeDBi P50396.
    TreeFami TF300449.

    Enzyme and pathway databases

    Reactomei REACT_210090. Rho GTPase cycle.

    Miscellaneous databases

    NextBioi 286278.
    PROi P50396.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P50396.
    Bgeei P50396.
    Genevestigatori P50396.

    Family and domain databases

    Gene3Di 3.40.50.720. 2 hits.
    InterProi IPR018203. GDP_dissociation_inhibitor.
    IPR016040. NAD(P)-bd_dom.
    IPR000806. RabGDI.
    [Graphical view ]
    Pfami PF00996. GDI. 1 hit.
    [Graphical view ]
    PRINTSi PR00892. RABGDI.
    PR00891. RABGDIREP.
    ProtoNeti Search...

    Publicationsi

    1. "Role of rab GDP dissociation inhibitor alpha in regulating plasticity of hippocampal neurotransmission."
      Ishizaki H., Miyoshi J., Kamiya H., Togawa A., Tanaka M., Sasaki T., Endo K., Mizoguchi A., Ozawa S., Takai Y.
      Proc. Natl. Acad. Sci. U.S.A. 97:11587-11592(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    2. "Knockout mice carrying a deletion of the mental retardation gene Gdi1 show impaired associative memory and inappropriate social behavior."
      D'Adamo P., Welzl H., Papadimitriou S., Raffaele di Barletta M., Tiveron C., Tatangelo L., Chapman P.F., Knevett S.G., Ramsay M.F., Valtorta F., Leoni C., Menegon A., Wolfer D.P., Lipp H.-P., Toniolo D.
      Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvEv.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Skin and Spleen.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and FVB/N.
      Tissue: Eye and Mammary tumor.
    6. Lubec G., Klug S., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 36-54; 56-68; 104-112; 119-137; 143-169; 174-208; 211-218; 222-240; 300-328; 349-379 AND 424-447, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain and Hippocampus.
    7. "Cloning, characterization, and expression of a novel GDP dissociation inhibitor isoform from skeletal muscle."
      Shisheva A., Suedhof T.C., Czech M.P.
      Mol. Cell. Biol. 14:3459-3468(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 125-447.
      Strain: BALB/c.
      Tissue: Skeletal muscle.
    8. "GDI-1 preferably interacts with Rab10 in insulin-stimulated GLUT4 translocation."
      Chen Y., Deng Y., Zhang J., Yang L., Xie X., Xu T.
      Biochem. J. 422:229-235(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RAB10.

    Entry informationi

    Entry nameiGDIA_MOUSE
    AccessioniPrimary (citable) accession number: P50396
    Secondary accession number(s): A2AMA8
    , Q3TBI9, Q8VHM3, Q91Y71, Q91Z41, Q96CX5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 112 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3