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P50393

- PA24A_RAT

UniProt

P50393 - PA24A_RAT

Protein

Cytosolic phospholipase A2

Gene

Pla2g4a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

    Catalytic activityi

    Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

    Enzyme regulationi

    Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi40 – 401Calcium 1By similarity
    Metal bindingi40 – 401Calcium 2By similarity
    Metal bindingi41 – 411Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi43 – 431Calcium 1By similarity
    Metal bindingi43 – 431Calcium 2By similarity
    Metal bindingi65 – 651Calcium 1By similarity
    Metal bindingi93 – 931Calcium 2By similarity
    Metal bindingi94 – 941Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi95 – 951Calcium 2By similarity
    Active sitei228 – 2281NucleophileBy similarity
    Active sitei549 – 5491Proton acceptorBy similarity

    GO - Molecular functioni

    1. calcium-dependent phospholipase A2 activity Source: RGD
    2. calcium-dependent phospholipid binding Source: UniProtKB
    3. calcium ion binding Source: UniProtKB
    4. lysophospholipase activity Source: UniProtKB-EC
    5. phospholipase A2 activity Source: RGD

    GO - Biological processi

    1. aging Source: RGD
    2. arachidonic acid metabolic process Source: RGD
    3. decidualization Source: RGD
    4. luteolysis Source: RGD
    5. ovulation from ovarian follicle Source: RGD
    6. phospholipid catabolic process Source: InterPro
    7. positive regulation of apoptotic process Source: RGD
    8. positive regulation of bone mineralization Source: RGD
    9. positive regulation of cell proliferation Source: RGD
    10. positive regulation of fever generation Source: RGD
    11. positive regulation of inflammatory response Source: RGD
    12. positive regulation of prostaglandin biosynthetic process Source: RGD
    13. positive regulation of vesicle fusion Source: RGD
    14. response to calcium ion Source: RGD
    15. response to glucocorticoid Source: RGD
    16. response to heat Source: RGD
    17. response to hormone Source: RGD
    18. response to hydrogen peroxide Source: RGD
    19. response to lipopolysaccharide Source: RGD
    20. response to methylmercury Source: RGD
    21. response to organic substance Source: RGD
    22. response to organonitrogen compound Source: RGD
    23. response to vitamin D Source: RGD
    24. surfactant homeostasis Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosolic phospholipase A2
    Short name:
    cPLA2
    Alternative name(s):
    Phospholipase A2 group IVA
    Including the following 2 domains:
    Phospholipase A2 (EC:3.1.1.4)
    Alternative name(s):
    Phosphatidylcholine 2-acylhydrolase
    Lysophospholipase (EC:3.1.1.5)
    Gene namesi
    Name:Pla2g4a
    Synonyms:Cpla2, Pla2g4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi67366. Pla2g4a.

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmic vesicle By similarity
    Note: Translocates to membrane vesicles in a calcium-dependent fashion.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. cytosol Source: RGD
    3. intracellular membrane-bounded organelle Source: RGD
    4. nucleus Source: RGD
    5. perinuclear region of cytoplasm Source: RGD
    6. zymogen granule Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 752752Cytosolic phospholipase A2PRO_0000187264Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei268 – 2681PhosphothreonineBy similarity
    Modified residuei437 – 4371PhosphoserineBy similarity
    Modified residuei505 – 5051Phosphoserine; by MAPKBy similarity
    Modified residuei727 – 7271PhosphoserineBy similarity
    Modified residuei729 – 7291PhosphoserineBy similarity

    Post-translational modificationi

    Activated by phosphorylation at both Ser-505 and Ser-727.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP50393.

    PTM databases

    PhosphoSiteiP50393.

    Expressioni

    Tissue specificityi

    In brain tissue, expressed in low levels in olfactory mitral and granule cells, in hippocampal pyramidal cells and in dentate and cerebellar granule cells.

    Inductioni

    Levels of rat CPLA2 are increased in dentate granule cells during ischemia.

    Gene expression databases

    GenevestigatoriP50393.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000003630.

    Structurei

    3D structure databases

    ProteinModelPortaliP50393.
    SMRiP50393. Positions 13-721.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 106102C2PROSITE-ProRule annotationAdd
    BLAST
    Domaini140 – 740601PLA2cPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 178178Phospholipid bindingCuratedAdd
    BLAST

    Domaini

    The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ By similarity.By similarity

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 PLA2c domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG257248.
    HOGENOMiHOG000115420.
    HOVERGENiHBG053479.
    InParanoidiP50393.
    PhylomeDBiP50393.

    Family and domain databases

    Gene3Di2.60.40.150. 1 hit.
    InterProiIPR016035. Acyl_Trfase/lysoPLipase.
    IPR000008. C2_dom.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view]
    PfamiPF00168. C2. 1 hit.
    PF01735. PLA2_B. 1 hit.
    [Graphical view]
    SMARTiSM00239. C2. 1 hit.
    SM00022. PLAc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF52151. SSF52151. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS51210. PLA2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50393-1 [UniParc]FASTAAdd to Basket

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    MSFIDPYQHI IVEHQYSHKF TVVVLRATKV TKGTFGDMLD TPDPYVELFI    50
    STTPDSRKRT RHFNNDINPV WNETFEFILD PNQENVLEIT LMDANYVMDE 100
    TLGTATFPVS SMKVGEKKEV PFIFNQVTEM ILEMSLEVCS CPDLRFSMAL 150
    CDQEKTFRRQ RKENIKENMK KLLGPKKSEG LYSTRDVPVV AILGSGGGFR 200
    AMVGFSGVMK ALYESGILDC ATYVAGLSGS TWYMSTLYSH PDFPEKGPEE 250
    INEELMKNVS HNPLLLLTPQ KVKRYVESLW KKKSSGQPVT FTDIFGMLIG 300
    ETLIQNRMST TLSSLKEKVS AARCPLPLFT CLHVKPDVSE LMFADWVEFS 350
    PYEIGMAKYG TFMTPDLFGS KFFMGTVVKK YEENPLHFLM GVWGSAFSIL 400
    FNRVLGVSGS QNKGSTMEEE LENITAKHIV SNDSSDSDDE AQGPKGTENE 450
    DAEREYQNDN QASWVHRMLM ALVSDSALFN TREGRAGKEH NFMLGLNLNT 500
    SYPLSPLRDF SPQDSFDDDE LDAAVADPDE FERIYEPLDV KSKKIHVVDS 550
    GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDTSPPFKEL LLAEKWAKMN 600
    KLPFPKIDPY VFDREGLKEC YVFKPKNPDV EKDCPTIIHF VLANINFRKY 650
    KAPGVLRETK EEKEIADFDI FDDPESPFST FNFQYPNQAF KRLHDLMYFN 700
    TLNNIDVIKD AIVESIEYRR QNPSRCSVSL SNVEARKFFN KEFLSKPTAE 750
    SI 752
    Length:752
    Mass (Da):85,707
    Last modified:October 1, 1996 - v1
    Checksum:iC68F71BB05FBF732
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti139 – 1391C → S in AAC21591. (PubMed:9555100)Curated
    Sequence conflicti159 – 1591R → Q in AAC21591. (PubMed:9555100)Curated
    Sequence conflicti287 – 2871Q → L in AAC21591. (PubMed:9555100)Curated
    Sequence conflicti308 – 3103MST → IVP in AAC21591. (PubMed:9555100)Curated
    Sequence conflicti410 – 4101S → L in AAC21591. (PubMed:9555100)Curated
    Sequence conflicti489 – 4891E → V in AAC21591. (PubMed:9555100)Curated
    Sequence conflicti635 – 6351P → T in AAC21591. (PubMed:9555100)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S77829 mRNA. Translation: AAB33847.1.
    U38376 mRNA. Translation: AAC21591.1.
    UniGeneiRn.10162.

    Genome annotation databases

    UCSCiRGD:67366. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S77829 mRNA. Translation: AAB33847.1 .
    U38376 mRNA. Translation: AAC21591.1 .
    UniGenei Rn.10162.

    3D structure databases

    ProteinModelPortali P50393.
    SMRi P50393. Positions 13-721.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000003630.

    PTM databases

    PhosphoSitei P50393.

    Proteomic databases

    PRIDEi P50393.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:67366. rat.

    Organism-specific databases

    RGDi 67366. Pla2g4a.

    Phylogenomic databases

    eggNOGi NOG257248.
    HOGENOMi HOG000115420.
    HOVERGENi HBG053479.
    InParanoidi P50393.
    PhylomeDBi P50393.

    Miscellaneous databases

    PROi P50393.

    Gene expression databases

    Genevestigatori P50393.

    Family and domain databases

    Gene3Di 2.60.40.150. 1 hit.
    InterProi IPR016035. Acyl_Trfase/lysoPLipase.
    IPR000008. C2_dom.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view ]
    Pfami PF00168. C2. 1 hit.
    PF01735. PLA2_B. 1 hit.
    [Graphical view ]
    SMARTi SM00239. C2. 1 hit.
    SM00022. PLAc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF52151. SSF52151. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS51210. PLA2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of rat cDNA for cytosolic phospholipase A2 and the increased gene expression in the dentate gyrus following transient forebrain ischemia."
      Owada Y., Tominaga T., Yoshimoto T., Kondo H.
      Brain Res. Mol. Brain Res. 25:364-368(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Cloning and expression of a group IV cytosolic Ca2+-dependent phospholipase A2 from rat pancreatic islets. Comparison of the expressed activity with that of an islet group VI cytosolic Ca2+-independent phospholipase A2."
      Ma Z., Ramanadham S., Hu Z., Turk J.
      Biochim. Biophys. Acta 1391:384-400(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Pancreatic islet.

    Entry informationi

    Entry nameiPA24A_RAT
    AccessioniPrimary (citable) accession number: P50393
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3