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P50393

- PA24A_RAT

UniProt

P50393 - PA24A_RAT

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Protein

Cytosolic phospholipase A2

Gene

Pla2g4a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Calcium 1By similarity
Metal bindingi40 – 401Calcium 2By similarity
Metal bindingi41 – 411Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi43 – 431Calcium 1By similarity
Metal bindingi43 – 431Calcium 2By similarity
Metal bindingi65 – 651Calcium 1By similarity
Metal bindingi93 – 931Calcium 2By similarity
Metal bindingi94 – 941Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi95 – 951Calcium 2By similarity
Active sitei228 – 2281NucleophileBy similarity
Active sitei549 – 5491Proton acceptorBy similarity

GO - Molecular functioni

  1. calcium-dependent phospholipase A2 activity Source: RGD
  2. calcium-dependent phospholipid binding Source: UniProtKB
  3. calcium ion binding Source: UniProtKB
  4. lysophospholipase activity Source: UniProtKB-EC
  5. phospholipase A2 activity Source: RGD

GO - Biological processi

  1. aging Source: RGD
  2. arachidonic acid metabolic process Source: RGD
  3. decidualization Source: RGD
  4. luteolysis Source: RGD
  5. ovulation from ovarian follicle Source: RGD
  6. phospholipid catabolic process Source: InterPro
  7. positive regulation of apoptotic process Source: RGD
  8. positive regulation of bone mineralization Source: RGD
  9. positive regulation of cell proliferation Source: RGD
  10. positive regulation of fever generation Source: RGD
  11. positive regulation of inflammatory response Source: RGD
  12. positive regulation of prostaglandin biosynthetic process Source: RGD
  13. positive regulation of vesicle fusion Source: RGD
  14. response to calcium ion Source: RGD
  15. response to glucocorticoid Source: RGD
  16. response to heat Source: RGD
  17. response to hormone Source: RGD
  18. response to hydrogen peroxide Source: RGD
  19. response to lipopolysaccharide Source: RGD
  20. response to lithium ion Source: RGD
  21. response to methylmercury Source: RGD
  22. response to organic substance Source: RGD
  23. response to organonitrogen compound Source: RGD
  24. response to vitamin D Source: RGD
  25. surfactant homeostasis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:Pla2g4a
Synonyms:Cpla2, Pla2g4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi67366. Pla2g4a.

Subcellular locationi

Cytoplasm By similarity. Cytoplasmic vesicle By similarity
Note: Translocates to membrane vesicles in a calcium-dependent fashion.By similarity

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. cytosol Source: RGD
  3. intracellular membrane-bounded organelle Source: RGD
  4. nucleus Source: RGD
  5. perinuclear region of cytoplasm Source: RGD
  6. zymogen granule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 752752Cytosolic phospholipase A2PRO_0000187264Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei268 – 2681PhosphothreonineBy similarity
Modified residuei437 – 4371PhosphoserineBy similarity
Modified residuei505 – 5051Phosphoserine; by MAPKBy similarity
Modified residuei727 – 7271PhosphoserineBy similarity
Modified residuei729 – 7291PhosphoserineBy similarity

Post-translational modificationi

Activated by phosphorylation at both Ser-505 and Ser-727.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP50393.

PTM databases

PhosphoSiteiP50393.

Expressioni

Tissue specificityi

In brain tissue, expressed in low levels in olfactory mitral and granule cells, in hippocampal pyramidal cells and in dentate and cerebellar granule cells.

Inductioni

Levels of rat CPLA2 are increased in dentate granule cells during ischemia.

Gene expression databases

GenevestigatoriP50393.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003630.

Structurei

3D structure databases

ProteinModelPortaliP50393.
SMRiP50393. Positions 13-721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 106102C2PROSITE-ProRule annotationAdd
BLAST
Domaini140 – 740601PLA2cPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 178178Phospholipid bindingCuratedAdd
BLAST

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG257248.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiP50393.
PhylomeDBiP50393.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50393-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFIDPYQHI IVEHQYSHKF TVVVLRATKV TKGTFGDMLD TPDPYVELFI
60 70 80 90 100
STTPDSRKRT RHFNNDINPV WNETFEFILD PNQENVLEIT LMDANYVMDE
110 120 130 140 150
TLGTATFPVS SMKVGEKKEV PFIFNQVTEM ILEMSLEVCS CPDLRFSMAL
160 170 180 190 200
CDQEKTFRRQ RKENIKENMK KLLGPKKSEG LYSTRDVPVV AILGSGGGFR
210 220 230 240 250
AMVGFSGVMK ALYESGILDC ATYVAGLSGS TWYMSTLYSH PDFPEKGPEE
260 270 280 290 300
INEELMKNVS HNPLLLLTPQ KVKRYVESLW KKKSSGQPVT FTDIFGMLIG
310 320 330 340 350
ETLIQNRMST TLSSLKEKVS AARCPLPLFT CLHVKPDVSE LMFADWVEFS
360 370 380 390 400
PYEIGMAKYG TFMTPDLFGS KFFMGTVVKK YEENPLHFLM GVWGSAFSIL
410 420 430 440 450
FNRVLGVSGS QNKGSTMEEE LENITAKHIV SNDSSDSDDE AQGPKGTENE
460 470 480 490 500
DAEREYQNDN QASWVHRMLM ALVSDSALFN TREGRAGKEH NFMLGLNLNT
510 520 530 540 550
SYPLSPLRDF SPQDSFDDDE LDAAVADPDE FERIYEPLDV KSKKIHVVDS
560 570 580 590 600
GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDTSPPFKEL LLAEKWAKMN
610 620 630 640 650
KLPFPKIDPY VFDREGLKEC YVFKPKNPDV EKDCPTIIHF VLANINFRKY
660 670 680 690 700
KAPGVLRETK EEKEIADFDI FDDPESPFST FNFQYPNQAF KRLHDLMYFN
710 720 730 740 750
TLNNIDVIKD AIVESIEYRR QNPSRCSVSL SNVEARKFFN KEFLSKPTAE

SI
Length:752
Mass (Da):85,707
Last modified:October 1, 1996 - v1
Checksum:iC68F71BB05FBF732
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti139 – 1391C → S in AAC21591. (PubMed:9555100)Curated
Sequence conflicti159 – 1591R → Q in AAC21591. (PubMed:9555100)Curated
Sequence conflicti287 – 2871Q → L in AAC21591. (PubMed:9555100)Curated
Sequence conflicti308 – 3103MST → IVP in AAC21591. (PubMed:9555100)Curated
Sequence conflicti410 – 4101S → L in AAC21591. (PubMed:9555100)Curated
Sequence conflicti489 – 4891E → V in AAC21591. (PubMed:9555100)Curated
Sequence conflicti635 – 6351P → T in AAC21591. (PubMed:9555100)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S77829 mRNA. Translation: AAB33847.1.
U38376 mRNA. Translation: AAC21591.1.
UniGeneiRn.10162.

Genome annotation databases

UCSCiRGD:67366. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S77829 mRNA. Translation: AAB33847.1 .
U38376 mRNA. Translation: AAC21591.1 .
UniGenei Rn.10162.

3D structure databases

ProteinModelPortali P50393.
SMRi P50393. Positions 13-721.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000003630.

PTM databases

PhosphoSitei P50393.

Proteomic databases

PRIDEi P50393.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:67366. rat.

Organism-specific databases

RGDi 67366. Pla2g4a.

Phylogenomic databases

eggNOGi NOG257248.
HOGENOMi HOG000115420.
HOVERGENi HBG053479.
InParanoidi P50393.
PhylomeDBi P50393.

Miscellaneous databases

PROi P50393.

Gene expression databases

Genevestigatori P50393.

Family and domain databases

Gene3Di 2.60.40.150. 1 hit.
InterProi IPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view ]
Pfami PF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view ]
SMARTi SM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of rat cDNA for cytosolic phospholipase A2 and the increased gene expression in the dentate gyrus following transient forebrain ischemia."
    Owada Y., Tominaga T., Yoshimoto T., Kondo H.
    Brain Res. Mol. Brain Res. 25:364-368(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Cloning and expression of a group IV cytosolic Ca2+-dependent phospholipase A2 from rat pancreatic islets. Comparison of the expressed activity with that of an islet group VI cytosolic Ca2+-independent phospholipase A2."
    Ma Z., Ramanadham S., Hu Z., Turk J.
    Biochim. Biophys. Acta 1391:384-400(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Pancreatic islet.

Entry informationi

Entry nameiPA24A_RAT
AccessioniPrimary (citable) accession number: P50393
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3