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Protein

Cytosolic phospholipase A2

Gene

Pla2g4a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Calcium 1By similarity1
Metal bindingi40Calcium 2By similarity1
Metal bindingi41Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi43Calcium 1By similarity1
Metal bindingi43Calcium 2By similarity1
Metal bindingi65Calcium 1By similarity1
Metal bindingi93Calcium 2By similarity1
Metal bindingi94Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi95Calcium 2By similarity1
Active sitei228NucleophileBy similarity1
Active sitei549Proton acceptorBy similarity1

GO - Molecular functioni

  • calcium-dependent phospholipase A2 activity Source: RGD
  • calcium-dependent phospholipid binding Source: UniProtKB
  • calcium ion binding Source: UniProtKB
  • histone acetyltransferase binding Source: RGD
  • lysophospholipase activity Source: UniProtKB-EC
  • phospholipase A2 activity Source: RGD

GO - Biological processi

  • aging Source: RGD
  • arachidonic acid metabolic process Source: RGD
  • decidualization Source: RGD
  • luteolysis Source: RGD
  • ovulation from ovarian follicle Source: RGD
  • phospholipid catabolic process Source: InterPro
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of bone mineralization Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of fever generation Source: RGD
  • positive regulation of inflammatory response Source: RGD
  • positive regulation of prostaglandin biosynthetic process Source: RGD
  • positive regulation of vesicle fusion Source: RGD
  • response to calcium ion Source: RGD
  • response to glucocorticoid Source: RGD
  • response to heat Source: RGD
  • response to hormone Source: RGD
  • response to hydrogen peroxide Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to lithium ion Source: RGD
  • response to methylmercury Source: RGD
  • response to organic substance Source: RGD
  • response to organonitrogen compound Source: RGD
  • response to vitamin D Source: RGD
  • surfactant homeostasis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:Pla2g4a
Synonyms:Cpla2, Pla2g4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi67366. Pla2g4a.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmic vesicle By similarity

  • Note: Translocates to membrane vesicles in a calcium-dependent fashion.By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • intracellular membrane-bounded organelle Source: RGD
  • nucleus Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • zymogen granule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872641 – 752Cytosolic phospholipase A2Add BLAST752

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2PhosphoserineBy similarity1
Modified residuei268PhosphothreonineBy similarity1
Modified residuei434PhosphoserineCombined sources1
Modified residuei435PhosphoserineBy similarity1
Modified residuei437PhosphoserineCombined sources1
Modified residuei505Phosphoserine; by MAPKBy similarity1
Modified residuei511PhosphoserineCombined sources1
Modified residuei515PhosphoserineCombined sources1
Modified residuei727PhosphoserineCombined sources1
Modified residuei729PhosphoserineBy similarity1

Post-translational modificationi

Activated by phosphorylation at both Ser-505 and Ser-727.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP50393.
PRIDEiP50393.

PTM databases

iPTMnetiP50393.
PhosphoSitePlusiP50393.

Expressioni

Tissue specificityi

In brain tissue, expressed in low levels in olfactory mitral and granule cells, in hippocampal pyramidal cells and in dentate and cerebellar granule cells.

Inductioni

Levels of rat CPLA2 are increased in dentate granule cells during ischemia.

Interactioni

GO - Molecular functioni

  • histone acetyltransferase binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003630.

Structurei

3D structure databases

ProteinModelPortaliP50393.
SMRiP50393.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 106C2PROSITE-ProRule annotationAdd BLAST102
Domaini140 – 740PLA2cPROSITE-ProRule annotationAdd BLAST601

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 178Phospholipid bindingCuratedAdd BLAST178

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1012. Eukaryota.
KOG1325. Eukaryota.
ENOG410XR72. LUCA.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiP50393.
PhylomeDBiP50393.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50393-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFIDPYQHI IVEHQYSHKF TVVVLRATKV TKGTFGDMLD TPDPYVELFI
60 70 80 90 100
STTPDSRKRT RHFNNDINPV WNETFEFILD PNQENVLEIT LMDANYVMDE
110 120 130 140 150
TLGTATFPVS SMKVGEKKEV PFIFNQVTEM ILEMSLEVCS CPDLRFSMAL
160 170 180 190 200
CDQEKTFRRQ RKENIKENMK KLLGPKKSEG LYSTRDVPVV AILGSGGGFR
210 220 230 240 250
AMVGFSGVMK ALYESGILDC ATYVAGLSGS TWYMSTLYSH PDFPEKGPEE
260 270 280 290 300
INEELMKNVS HNPLLLLTPQ KVKRYVESLW KKKSSGQPVT FTDIFGMLIG
310 320 330 340 350
ETLIQNRMST TLSSLKEKVS AARCPLPLFT CLHVKPDVSE LMFADWVEFS
360 370 380 390 400
PYEIGMAKYG TFMTPDLFGS KFFMGTVVKK YEENPLHFLM GVWGSAFSIL
410 420 430 440 450
FNRVLGVSGS QNKGSTMEEE LENITAKHIV SNDSSDSDDE AQGPKGTENE
460 470 480 490 500
DAEREYQNDN QASWVHRMLM ALVSDSALFN TREGRAGKEH NFMLGLNLNT
510 520 530 540 550
SYPLSPLRDF SPQDSFDDDE LDAAVADPDE FERIYEPLDV KSKKIHVVDS
560 570 580 590 600
GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDTSPPFKEL LLAEKWAKMN
610 620 630 640 650
KLPFPKIDPY VFDREGLKEC YVFKPKNPDV EKDCPTIIHF VLANINFRKY
660 670 680 690 700
KAPGVLRETK EEKEIADFDI FDDPESPFST FNFQYPNQAF KRLHDLMYFN
710 720 730 740 750
TLNNIDVIKD AIVESIEYRR QNPSRCSVSL SNVEARKFFN KEFLSKPTAE

SI
Length:752
Mass (Da):85,707
Last modified:October 1, 1996 - v1
Checksum:iC68F71BB05FBF732
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139C → S in AAC21591 (PubMed:9555100).Curated1
Sequence conflicti159R → Q in AAC21591 (PubMed:9555100).Curated1
Sequence conflicti287Q → L in AAC21591 (PubMed:9555100).Curated1
Sequence conflicti308 – 310MST → IVP in AAC21591 (PubMed:9555100).Curated3
Sequence conflicti410S → L in AAC21591 (PubMed:9555100).Curated1
Sequence conflicti489E → V in AAC21591 (PubMed:9555100).Curated1
Sequence conflicti635P → T in AAC21591 (PubMed:9555100).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S77829 mRNA. Translation: AAB33847.1.
U38376 mRNA. Translation: AAC21591.1.
UniGeneiRn.10162.

Genome annotation databases

UCSCiRGD:67366. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S77829 mRNA. Translation: AAB33847.1.
U38376 mRNA. Translation: AAC21591.1.
UniGeneiRn.10162.

3D structure databases

ProteinModelPortaliP50393.
SMRiP50393.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003630.

PTM databases

iPTMnetiP50393.
PhosphoSitePlusiP50393.

Proteomic databases

PaxDbiP50393.
PRIDEiP50393.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:67366. rat.

Organism-specific databases

RGDi67366. Pla2g4a.

Phylogenomic databases

eggNOGiKOG1012. Eukaryota.
KOG1325. Eukaryota.
ENOG410XR72. LUCA.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiP50393.
PhylomeDBiP50393.

Miscellaneous databases

PROiP50393.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA24A_RAT
AccessioniPrimary (citable) accession number: P50393
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.