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Protein

Cytosolic phospholipase A2

Gene

pla2g4a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Calcium 1By similarity1
Metal bindingi34Calcium 2By similarity1
Metal bindingi35Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi37Calcium 1By similarity1
Metal bindingi37Calcium 2By similarity1
Metal bindingi59Calcium 1By similarity1
Metal bindingi87Calcium 2By similarity1
Metal bindingi88Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi89Calcium 2By similarity1
Active sitei223NucleophileBy similarity1
Active sitei540Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • inflammatory response to wounding Source: ZFIN
  • ovarian follicle development Source: ZFIN
  • phospholipid catabolic process Source: InterPro
  • regulation of G1/S transition of mitotic cell cycle Source: ZFIN
  • response to wounding Source: ZFIN

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:pla2g4a
Synonyms:cpla2, pla2g4
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-990415-45 pla2g4aa

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872651 – 741Cytosolic phospholipase A2Add BLAST741

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei498Phosphoserine; by MAPKBy similarity1

Post-translational modificationi

Activated by phosphorylation on a serine residue.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP50392
PRIDEiP50392

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000090686

Structurei

3D structure databases

ProteinModelPortaliP50392
SMRiP50392
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 100C2PROSITE-ProRule annotationAdd BLAST100
Domaini132 – 729PLA2cPROSITE-ProRule annotationAdd BLAST598

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 172Phospholipid bindingCuratedAdd BLAST172

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Phylogenomic databases

eggNOGiKOG1012 Eukaryota
KOG1325 Eukaryota
ENOG410XR72 LUCA
HOGENOMiHOG000115420
HOVERGENiHBG053479
InParanoidiP50392
KOiK16342
PhylomeDBiP50392

Family and domain databases

Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR016035 Acyl_Trfase/lysoPLipase
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR002642 LysoPLipase_cat_dom
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF01735 PLA2_B, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00022 PLAc, 1 hit
SUPFAMiSSF52151 SSF52151, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS51210 PLA2C, 1 hit

Sequencei

Sequence statusi: Complete.

P50392-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNIIVEHQY SHRLKLTVVR AENVTKGAFG DLLDTPDPYV ELSVPTTPES
60 70 80 90 100
RKRTRHINND INPKWNETFE FILDPNQSNV LEVTLMDANY VMDETLGTAK
110 120 130 140 150
YSLSKLKVAQ MEHVTLSIGK TTKVFLDLLL EVCASTDLRF SMTLCDQEKL
160 170 180 190 200
FMQTRRDRVM LSIKKLLKME NPRFLPSSPR EVPTIAILGS GGGFRAMVGF
210 220 230 240 250
SGVMKALYES GVFDCATYVA GLSGSTWYMS MLYSHPEFPA KGPGDINKEL
260 270 280 290 300
MNRVSNNPLK LLLPQNINRY VKALWKKKSA GQPVTFTDIF GMLIGETLIP
310 320 330 340 350
GRMNIKLSSL KGKINEGQSP LPLFTCLHVK PDVSELMFAD WVEFSPYEIG
360 370 380 390 400
MAKYGTFMSP GLFGSKFFMG SVVKQYEENP LHFLMGVWGS AFSILFNRVL
410 420 430 440 450
GVKETTSSST MEEELEQIKP EHIVGDDSAD NEEETQRGGT ESADAEDERQ
460 470 480 490 500
RHAQASWVQR MLTSIMGDTT LFTTREGRAG KVHNFMLGLN LNSTLPFSPF
510 520 530 540 550
SGITHQTSLE EEVDAVTDPD EFERIYEPLD VKSKKIHVVD SGLTFNLPYP
560 570 580 590 600
LILRCQRGVD LIISFDFSAR PSDSSPPFKE LLLAEKWARM NKLPFPKIDS
610 620 630 640 650
KVFDREGLKE CYVFKPAKGD KNCPTIIHFV LANINFRNFK APGVPRDSDK
660 670 680 690 700
DIEFGDFDIF DEPASPYSTF NFKYNNQAFK RLHDLMEFNT LNNIEVIKEA
710 720 730 740
IKDSILLRRE NPARCSVSLS LSEIENKKFL KRDNSIAKRP T
Length:741
Mass (Da):83,809
Last modified:October 1, 1996 - v1
Checksum:i34896B1A8364A9D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10330 mRNA Translation: AAA53229.1
PIRiB54908
RefSeqiNP_571370.1, NM_131295.2
UniGeneiDr.20961

Genome annotation databases

GeneIDi30554
KEGGidre:30554

Similar proteinsi

Entry informationi

Entry nameiPA24A_DANRE
AccessioniPrimary (citable) accession number: P50392
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health