P50391 (NPY4R_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuropeptide Y receptor type 4
Short name=NPY4-R

Alternative name(s):
Pancreatic polypeptide receptor 1
Short name=PP1

Gene names

Name:	PPYR1
Synonyms:	NPY4R

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PP, PP (2-36) and [Ile-31, Gln-34] PP > [Pro-34] PYY > PYY and [Leu-31, Pro-34] NPY > NPY > PYY (3-36) and NPY (2-36) > PP (13-36) > PP (31-36) > NPY free acid.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Highest levels found in brain, coronary artery and ileum. Low levels in pancreas and kidney. Detected in colon and small intestine.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood circulation Traceable author statement Ref.1. Source: ProtInc digestion Traceable author statement Ref.1. Source: ProtInc feeding behavior Traceable author statement Ref.1. Source: ProtInc
Cellular_component	integral to plasma membrane Traceable author statement Ref.3. Source: ProtInc
Molecular_function	G-protein coupled receptor activity Traceable author statement Ref.3. Source: ProtInc pancreatic polypeptide receptor activity Inferred from electronic annotation. Source: Compara peptide YY receptor activity Inferred from electronic annotation. Source: Compara peptide binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Neuropeptide Y receptor type 4		PRO_0000069935
Regions
	Topological domain	1 – 39	39	Extracellular Potential
	Transmembrane	40 – 60	21	Helical; Name=1; Potential
	Topological domain	61 – 87	27	Cytoplasmic Potential
	Transmembrane	88 – 108	21	Helical; Name=2; Potential
	Topological domain	109 – 116	8	Extracellular Potential
	Transmembrane	117 – 137	21	Helical; Name=3; Potential
	Topological domain	138 – 155	18	Cytoplasmic Potential
	Transmembrane	156 – 176	21	Helical; Name=4; Potential
	Topological domain	177 – 212	36	Extracellular Potential
	Transmembrane	213 – 233	21	Helical; Name=5; Potential
	Topological domain	234 – 263	30	Cytoplasmic Potential
	Transmembrane	264 – 284	21	Helical; Name=6; Potential
	Topological domain	285 – 301	17	Extracellular Potential
	Transmembrane	302 – 322	21	Helical; Name=7; Potential
	Topological domain	323 – 375	53	Cytoplasmic Potential
Amino acid modifications
	Lipidation	340	1	S-palmitoyl cysteine Potential
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	19	1	N-linked (GlcNAc...) Potential
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	187	1	N-linked (GlcNAc...) Potential
	Disulfide bond	114 ↔ 201		By similarity
Natural variations
	Natural variant	99	1	A → S. Ref.3 Ref.4 Ref.8 Corresponds to variant rs2229967 [ dbSNP | Ensembl ].		VAR_030337
	Natural variant	240	1	R → C. Ref.6 Corresponds to variant rs3824733 [ dbSNP | Ensembl ].		VAR_030338

Sequences

	Sequence		Length	Mass (Da)
	P50391 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 0081DBA51436EE22	FASTA	375	42,195
	10 20 30 40 50 60 MNTSHLLALL LPKSPQGENR SKPLGTPYNF SEHCQDSVDV MVFIVTSYSI ETVVGVLGNL 70 80 90 100 110 120 CLMCVTVRQK EKANVTNLLI ANLAFSDFLM CLLCQPLTAV YTIMDYWIFG ETLCKMSAFI 130 140 150 160 170 180 QCMSVTVSIL SLVLVALERH QLIINPTGWK PSISQAYLGI VLIWVIACVL SLPFLANSIL 190 200 210 220 230 240 ENVFHKNHSK ALEFLADKVV CTESWPLAHH RTIYTTFLLL FQYCLPLGFI LVCYARIYRR 250 260 270 280 290 300 LQRQGRVFHK GTYSLRAGHM KQVNVVLVVM VVAFAVLWLP LHVFNSLEDW HHEAIPICHG 310 320 330 340 350 360 NLIFLVCHLL AMASTCVNPF IYGFLNTNFK KEIKALVLTC QQSAPLEESE HLPLSTVHTE 370 VSKGSLRLSG RSNPI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and functional expression of a human Y4 subtype receptor for pancreatic polypeptide, neuropeptide Y, and peptide YY." Bard J.A., Walker M.W., Branchek T.A., Weinshank R.L. J. Biol. Chem. 270:26762-26765(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[2]	"Cloning of a human receptor of the NPY receptor family with high affinity for pancreatic polypeptide and peptide YY." Lundell I., Blomqvist A.G., Berglund M.M., Schober D.A., Johnson D., Statnick M.A., Gadski R.A., Gehlert D.R., Larhammar D. J. Biol. Chem. 270:29123-29128(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning and functional expression of cDNAs encoding human and rat pancreatic polypeptide receptors." Yan H., Yang J., Marasco J., Yamaguchi K., Brenner S., Collins F., Karbon W. Proc. Natl. Acad. Sci. U.S.A. 93:4661-4665(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-99.
[4]	"PPYR1: identification of new untranslated 5' exon by in silico and experimental evaluation of gene structure." Reichwald K., Petz U., Roschmann E., Platzer M. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-99.
[5]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-240.
[7]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-99.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U35232 Genomic DNA. Translation: AAC50280.1.
Z66526 Genomic DNA. Translation: CAA91433.1.
U42387 mRNA. Translation: AAB07759.1.
AY649985 mRNA. Translation: AAV68196.1.
AY649986 mRNA. Translation: AAV68197.1.
AY268432 Genomic DNA. Translation: AAP23199.1.
CR541950 mRNA. Translation: CAG46748.1.
AL390716 Genomic DNA. Translation: CAI13318.1.
BC096238 mRNA. Translation: AAH96238.1.

IPI

IPI00031457.

PIR

G02300.
I39182.

RefSeq

NP_005963.3. NM_005972.4.
XP_003846542.1. XM_003846494.2.
XP_003846799.1. XM_003846751.1.
XP_003960519.1. XM_003960470.1.

UniGene

Hs.524719.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IK5	model	-	@	1-375	[»]

ProteinModelPortal

P50391.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000363431.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P50391.

Polymorphism databases

DMDM

1709423.

Proteomic databases

PaxDb

P50391.

PRIDE

P50391.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000374312; ENSP00000363431; ENSG00000204174.
ENST00000395716; ENSP00000379066; ENSG00000204174.

GeneID

100996758.
101060446.
5540.

KEGG

hsa:100996758.
hsa:101060446.
hsa:5540.

UCSC

uc001jee.3. human.

Organism-specific databases

CTD

5540.

GeneCards

GC10P047083.

H-InvDB

HIX0170461.

HGNC

HGNC:9329. PPYR1.

HPA

HPA027863.

MIM

601790. gene.

neXtProt

NX_P50391.

PharmGKB

PA33692.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG261951.

HOGENOM

HOG000050229.

HOVERGEN

HBG105676.

InParanoid

P50391.

KO

K04204.

OMA

KSPQGEN.

OrthoDB

EOG4STS4V.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

Bgee

P50391.

CleanEx

HS_PPYR1.

Genevestigator

P50391.

GermOnline

ENSG00000204174. Homo sapiens.

Family and domain databases

InterPro

IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001933. NPY4_rcpt.
IPR000611. NPY_rcpt.
[Graphical view]

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR00237. GPCRRHODOPSN.
PR01015. NRPEPTIDEY4R.
PR01012. NRPEPTIDEYR.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P50391.

ChEMBL

CHEMBL4877.

NextBio

21462.

SOURCE

Search...

Entry information

Entry name

NPY4R_HUMAN

Accession

Primary (citable) accession number: P50391
Secondary accession number(s): Q13456 , Q5ISU3, Q5T2X9, Q6FH06

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.