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P50389

- PNPH_SULSO

UniProt

P50389 - PNPH_SULSO

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Protein

Purine nucleoside phosphorylase

Gene

SSO2706

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules. Cleaves inosine, guanosine, and adenosine with a better efficiency than MTA.2 Publications

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.
Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate.

Kineticsi

  1. KM=154 µM for S-methyl-5'-thioadenosine1 Publication
  2. KM=25.4 µM for adenosine1 Publication
  3. KM=84 µM for inosine1 Publication
  4. KM=113.6 µM for guanosine1 Publication

Temperature dependencei

Optimum temperature is 120 degrees Celsius. Highly thermostable.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei5 – 51Substrate; shared with dimeric partner
Binding sitei21 – 211Phosphate; via amide nitrogen
Binding sitei25 – 251Phosphate
Binding sitei43 – 431Phosphate; shared with dimeric partner
Binding sitei163 – 1631Substrate

GO - Molecular functioni

  1. purine-nucleoside phosphorylase activity Source: UniProtKB-EC

GO - Biological processi

  1. nucleoside metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-2504-MONOMER.
BRENDAi2.4.2.28. 6163.
UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Purine nucleoside phosphorylase (EC:2.4.2.1)
Short name:
PNP
Alternative name(s):
5'-methylthioadenosine phosphorylase I
Short name:
MTA phosphorylase I
Short name:
MTAPI
Gene namesi
Ordered Locus Names:SSO2706
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 236236Purine nucleoside phosphorylasePRO_0000063195Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi125 – 125Interchain

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homohexamer; disulfide-linked. Trimer of homodimers, with three symmetric intersubunit disulfide bonds linking the dimers to one another.2 Publications

Protein-protein interaction databases

STRINGi273057.SSO2706.

Structurei

Secondary structure

1
236
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 63Combined sources
Beta strandi15 – 217Combined sources
Helixi23 – 297Combined sources
Helixi30 – 323Combined sources
Beta strandi33 – 408Combined sources
Helixi42 – 443Combined sources
Beta strandi47 – 526Combined sources
Beta strandi55 – 617Combined sources
Helixi66 – 7813Combined sources
Beta strandi83 – 9210Combined sources
Beta strandi102 – 1109Combined sources
Helixi114 – 1207Combined sources
Helixi132 – 14413Combined sources
Beta strandi149 – 1568Combined sources
Helixi160 – 1623Combined sources
Helixi167 – 1726Combined sources
Turni173 – 1753Combined sources
Beta strandi176 – 1827Combined sources
Helixi183 – 19311Combined sources
Beta strandi196 – 20611Combined sources
Helixi218 – 23417Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JDSX-ray1.80A/B/C/D/E/F1-236[»]
1JDTX-ray2.00A/B/C1-236[»]
1JDUX-ray2.50A/B/C1-236[»]
1JDVX-ray2.00A/B/C/D/E/F1-236[»]
1JDZX-ray2.00A/B/C1-236[»]
1JE0X-ray1.60A/B/C1-236[»]
1JE1X-ray1.80A/B/C/D/E/F1-236[»]
1JP7X-ray1.80A/B/C1-236[»]
1JPVX-ray1.80A/B/C1-236[»]
ProteinModelPortaliP50389.
SMRiP50389. Positions 2-236.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50389.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni86 – 894Phosphate binding
Regioni180 – 1823Substrate binding
Regioni204 – 2052Substrate binding

Sequence similaritiesi

Belongs to the PNP/UDP phosphorylase family.Curated

Phylogenomic databases

eggNOGiCOG2820.
HOGENOMiHOG000274897.
InParanoidiP50389.
KOiK00772.
OMAiGEPDRAN.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
InterProiIPR018017. Nucleoside_phosphorylase.
IPR018016. Nucleoside_phosphorylase_CS.
IPR000845. Nucleoside_phosphorylase_d.
[Graphical view]
PANTHERiPTHR21234. PTHR21234. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
PROSITEiPS01232. PNP_UDP_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50389-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNPVHILAKK GEVAERVLVV GDPGRARLLS TLLQNPKLTN ENRGFLVYTG
60 70 80 90 100
KYNGETVSIA THGIGGPSIA IVLEELAMLG ANVFIRYGTT GALVPYINLG
110 120 130 140 150
EYIIVTGASY NQGGLFYQYL RDNACVASTP DFELTNKLVT SFSKRNLKYY
160 170 180 190 200
VGNVFSSDAF YAEDEEFVKK WSSRGNIAVE MECATLFTLS KVKGWKSATV
210 220 230
LVVSDNLAKG GIWITKEELE KSVMDGAKAV LDTLTS
Length:236
Mass (Da):25,738
Last modified:October 1, 1996 - v1
Checksum:iF1570ECE8AA3D51B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50181 Genomic DNA. Translation: CAA90560.1.
AE006641 Genomic DNA. Translation: AAK42818.1.
PIRiS58291.
RefSeqiNP_344028.1. NC_002754.1.
WP_009988635.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK42818; AAK42818; SSO2706.
GeneIDi1452737.
KEGGisso:SSO2706.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50181 Genomic DNA. Translation: CAA90560.1 .
AE006641 Genomic DNA. Translation: AAK42818.1 .
PIRi S58291.
RefSeqi NP_344028.1. NC_002754.1.
WP_009988635.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1JDS X-ray 1.80 A/B/C/D/E/F 1-236 [» ]
1JDT X-ray 2.00 A/B/C 1-236 [» ]
1JDU X-ray 2.50 A/B/C 1-236 [» ]
1JDV X-ray 2.00 A/B/C/D/E/F 1-236 [» ]
1JDZ X-ray 2.00 A/B/C 1-236 [» ]
1JE0 X-ray 1.60 A/B/C 1-236 [» ]
1JE1 X-ray 1.80 A/B/C/D/E/F 1-236 [» ]
1JP7 X-ray 1.80 A/B/C 1-236 [» ]
1JPV X-ray 1.80 A/B/C 1-236 [» ]
ProteinModelPortali P50389.
SMRi P50389. Positions 2-236.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273057.SSO2706.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK42818 ; AAK42818 ; SSO2706 .
GeneIDi 1452737.
KEGGi sso:SSO2706.

Phylogenomic databases

eggNOGi COG2820.
HOGENOMi HOG000274897.
InParanoidi P50389.
KOi K00772.
OMAi GEPDRAN.

Enzyme and pathway databases

UniPathwayi UPA00606 .
BioCyci SSOL273057:GCH2-2504-MONOMER.
BRENDAi 2.4.2.28. 6163.

Miscellaneous databases

EvolutionaryTracei P50389.

Family and domain databases

Gene3Di 3.40.50.1580. 1 hit.
InterProi IPR018017. Nucleoside_phosphorylase.
IPR018016. Nucleoside_phosphorylase_CS.
IPR000845. Nucleoside_phosphorylase_d.
[Graphical view ]
PANTHERi PTHR21234. PTHR21234. 1 hit.
Pfami PF01048. PNP_UDP_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53167. SSF53167. 1 hit.
PROSITEi PS01232. PNP_UDP_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus. Gene cloning and amino acid sequence determination."
    Cacciapuoti G., Porcelli M., Bertoldo C., Fusco S., De Rosa M., Zappia V.
    Eur. J. Biochem. 239:632-637(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  3. "Purification and characterization of extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus. Purine nucleoside phosphorylase activity and evidence for intersubunit disulfide bonds."
    Cacciapuoti G., Porcelli M., Bertoldo C., de Rosa M., Zappia V.
    J. Biol. Chem. 269:24762-24769(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-26, FUNCTION, SUBUNIT.
  4. "A novel hyperthermostable 5'-deoxy-5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus."
    Cacciapuoti G., Forte S., Moretti M.A., Brio A., Zappia V., Porcelli M.
    FEBS J. 272:1886-1899(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  5. "Three-dimensional structure of a hyperthermophilic 5'-deoxy-5'-methylthioadenosine phosphorylase from Sulfolobus solfataricus."
    Appleby T.C., Mathews I.I., Porcelli M., Cacciapuoti G., Ealick S.E.
    J. Biol. Chem. 276:39232-39242(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH SUBSTRATES AND SUBSTRATE ANALOGS.

Entry informationi

Entry nameiPNPH_SULSO
AccessioniPrimary (citable) accession number: P50389
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3