Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Purine nucleoside phosphorylase

Gene

SSO2706

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules. Cleaves inosine, guanosine, and adenosine with a better efficiency than MTA.2 Publications

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.2 Publications
Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.2 Publications
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate.1 Publication

Kineticsi

  1. KM=154 µM for S-methyl-5'-thioadenosine1 Publication
  2. KM=25.4 µM for adenosine1 Publication
  3. KM=84 µM for inosine1 Publication
  4. KM=113.6 µM for guanosine1 Publication

    Temperature dependencei

    Optimum temperature is 120 degrees Celsius. Highly thermostable.1 Publication

    Pathwayi: purine nucleoside salvage

    This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.
    View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei5Purine nucleoside; shared with dimeric partner1 Publication1
    Binding sitei21Phosphate; via amide nitrogenCombined sources1 Publication1
    Binding sitei25PhosphateCombined sources1 Publication1
    Binding sitei43Phosphate; shared with dimeric partnerCombined sources1 Publication1
    Binding sitei163Purine nucleoside1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciSSOL273057:GCH2-2505-MONOMER.
    BRENDAi2.4.2.28. 6163.
    UniPathwayiUPA00606.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purine nucleoside phosphorylase (EC:2.4.2.11 Publication)
    Short name:
    PNP
    Alternative name(s):
    5'-methylthioadenosine phosphorylase I1 Publication
    Short name:
    MTA phosphorylase I
    Short name:
    MTAPI
    Gene namesi
    Ordered Locus Names:SSO2706
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    Proteomesi
    • UP000001974 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000631951 – 236Purine nucleoside phosphorylaseAdd BLAST236

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi125InterchainCombined sources1 Publication

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Homohexamer; disulfide-linked. Trimer of homodimers, with three symmetric intersubunit disulfide bonds linking the dimers to one another.2 Publications

    Protein-protein interaction databases

    STRINGi273057.SSO2706.

    Structurei

    Secondary structure

    1236
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 6Combined sources3
    Beta strandi15 – 21Combined sources7
    Helixi23 – 29Combined sources7
    Helixi30 – 32Combined sources3
    Beta strandi33 – 40Combined sources8
    Helixi42 – 44Combined sources3
    Beta strandi47 – 52Combined sources6
    Beta strandi55 – 61Combined sources7
    Helixi66 – 78Combined sources13
    Beta strandi83 – 92Combined sources10
    Beta strandi102 – 110Combined sources9
    Helixi114 – 120Combined sources7
    Helixi132 – 144Combined sources13
    Beta strandi149 – 156Combined sources8
    Helixi160 – 162Combined sources3
    Helixi167 – 172Combined sources6
    Turni173 – 175Combined sources3
    Beta strandi176 – 182Combined sources7
    Helixi183 – 193Combined sources11
    Beta strandi196 – 206Combined sources11
    Helixi218 – 234Combined sources17

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1JDSX-ray1.80A/B/C/D/E/F1-236[»]
    1JDTX-ray2.00A/B/C1-236[»]
    1JDUX-ray2.50A/B/C1-236[»]
    1JDVX-ray2.00A/B/C/D/E/F1-236[»]
    1JDZX-ray2.00A/B/C1-236[»]
    1JE0X-ray1.60A/B/C1-236[»]
    1JE1X-ray1.80A/B/C/D/E/F1-236[»]
    1JP7X-ray1.80A/B/C1-236[»]
    1JPVX-ray1.80A/B/C1-236[»]
    ProteinModelPortaliP50389.
    SMRiP50389.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP50389.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni86 – 89Phosphate bindingCombined sources1 Publication4
    Regioni180 – 182Purine nucleoside bindingCombined sources1 Publication3
    Regioni204 – 205Purine nucleoside binding1 Publication2

    Sequence similaritiesi

    Belongs to the PNP/UDP phosphorylase family.Curated

    Phylogenomic databases

    eggNOGiarCOG01324. Archaea.
    COG2820. LUCA.
    HOGENOMiHOG000274897.
    InParanoidiP50389.
    KOiK00772.
    OMAiVECYNEV.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    InterProiIPR018017. Nucleoside_phosphorylase.
    IPR018016. Nucleoside_phosphorylase_CS.
    IPR000845. Nucleoside_phosphorylase_d.
    [Graphical view]
    PANTHERiPTHR21234. PTHR21234. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    PROSITEiPS01232. PNP_UDP_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50389-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNPVHILAKK GEVAERVLVV GDPGRARLLS TLLQNPKLTN ENRGFLVYTG
    60 70 80 90 100
    KYNGETVSIA THGIGGPSIA IVLEELAMLG ANVFIRYGTT GALVPYINLG
    110 120 130 140 150
    EYIIVTGASY NQGGLFYQYL RDNACVASTP DFELTNKLVT SFSKRNLKYY
    160 170 180 190 200
    VGNVFSSDAF YAEDEEFVKK WSSRGNIAVE MECATLFTLS KVKGWKSATV
    210 220 230
    LVVSDNLAKG GIWITKEELE KSVMDGAKAV LDTLTS
    Length:236
    Mass (Da):25,738
    Last modified:October 1, 1996 - v1
    Checksum:iF1570ECE8AA3D51B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z50181 Genomic DNA. Translation: CAA90560.1.
    AE006641 Genomic DNA. Translation: AAK42818.1.
    PIRiS58291.
    RefSeqiWP_009988635.1. NC_002754.1.

    Genome annotation databases

    EnsemblBacteriaiAAK42818; AAK42818; SSO2706.
    GeneIDi27428999.
    KEGGisso:SSO2706.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z50181 Genomic DNA. Translation: CAA90560.1.
    AE006641 Genomic DNA. Translation: AAK42818.1.
    PIRiS58291.
    RefSeqiWP_009988635.1. NC_002754.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1JDSX-ray1.80A/B/C/D/E/F1-236[»]
    1JDTX-ray2.00A/B/C1-236[»]
    1JDUX-ray2.50A/B/C1-236[»]
    1JDVX-ray2.00A/B/C/D/E/F1-236[»]
    1JDZX-ray2.00A/B/C1-236[»]
    1JE0X-ray1.60A/B/C1-236[»]
    1JE1X-ray1.80A/B/C/D/E/F1-236[»]
    1JP7X-ray1.80A/B/C1-236[»]
    1JPVX-ray1.80A/B/C1-236[»]
    ProteinModelPortaliP50389.
    SMRiP50389.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi273057.SSO2706.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAK42818; AAK42818; SSO2706.
    GeneIDi27428999.
    KEGGisso:SSO2706.

    Phylogenomic databases

    eggNOGiarCOG01324. Archaea.
    COG2820. LUCA.
    HOGENOMiHOG000274897.
    InParanoidiP50389.
    KOiK00772.
    OMAiVECYNEV.

    Enzyme and pathway databases

    UniPathwayiUPA00606.
    BioCyciSSOL273057:GCH2-2505-MONOMER.
    BRENDAi2.4.2.28. 6163.

    Miscellaneous databases

    EvolutionaryTraceiP50389.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    InterProiIPR018017. Nucleoside_phosphorylase.
    IPR018016. Nucleoside_phosphorylase_CS.
    IPR000845. Nucleoside_phosphorylase_d.
    [Graphical view]
    PANTHERiPTHR21234. PTHR21234. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    PROSITEiPS01232. PNP_UDP_1. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPNPH_SULSO
    AccessioniPrimary (citable) accession number: P50389
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: November 2, 2016
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.