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P50369

- PETL_CHLRE

UniProt

P50369 - PETL_CHLRE

Protein

Cytochrome b6-f complex subunit 6

Gene

petL

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. PetL is important for photoautotrophic growth as well as for electron transfer efficiency and stability of the cytochrome b6-f complex.UniRule annotation

    GO - Molecular functioni

    1. electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. oxidation-reduction process Source: UniProtKB-HAMAP
    2. photosynthesis Source: UniProtKB-KW

    Keywords - Biological processi

    Electron transport, Photosynthesis, Transport

    Enzyme and pathway databases

    BioCyciCHLAMY:CHRECP068-MONOMER.
    MetaCyc:CHRECP068-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome b6-f complex subunit 6UniRule annotation
    Alternative name(s):
    Cytochrome b6-f complex subunit PetLUniRule annotation
    Cytochrome b6-f complex subunit VIUniRule annotation
    Gene namesi
    Name:petLUniRule annotation
    Encoded oniPlastid; Chloroplast
    OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
    Taxonomic identifieri3055 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas
    ProteomesiUP000006906: Chloroplast

    Subcellular locationi

    Plastidchloroplast thylakoid membrane UniRule annotation; Single-pass membrane protein UniRule annotation

    GO - Cellular componenti

    1. chloroplast thylakoid membrane Source: UniProtKB-SubCell
    2. cytochrome b6f complex Source: InterPro
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Thylakoid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4343Cytochrome b6-f complex subunit 6PRO_0000220445Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.

    Interactioni

    Subunit structurei

    The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.UniRule annotation

    Structurei

    Secondary structure

    1
    43
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi13 – 3725

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Q90X-ray3.10L12-43[»]
    ProteinModelPortaliP50369.
    SMRiP50369. Positions 12-43.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP50369.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei17 – 3721HelicalUniRule annotationAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PetL family.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    HAMAPiMF_00433. Cytb6_f_PetL.
    InterProiIPR007802. Cyt_b6/f_cplx_su6.
    [Graphical view]
    PfamiPF05115. PetL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50369-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIFDFNYIHI FMLTITSYVG LLIGALVFTL GIYLGLLKVV KLI          43
    Length:43
    Mass (Da):4,878
    Last modified:July 11, 2003 - v3
    Checksum:iB7E6977ED788C9F3
    GO

    Sequence cautioni

    The sequence ACJ50155.1 differs from that shown. Reason: Erroneous initiation.

    Mass spectrometryi

    Molecular mass is 3467 Da from positions 1 - 30. Determined by MALDI. 1 Publication
    Molecular mass is 3918 Da from positions 5 - 39. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43964 Genomic DNA. Translation: AAB17716.1.
    FJ423446 Genomic DNA. Translation: ACJ50155.1. Different initiation.
    BK000554 Genomic DNA. Translation: DAA00968.1.
    PIRiT08024.
    RefSeqiNP_958424.2. NC_005353.1.

    Genome annotation databases

    EnsemblPlantsiDAA00968; DAA00968; DAA00968.
    GeneIDi2717047.
    KEGGicre:ChreCp068.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43964 Genomic DNA. Translation: AAB17716.1 .
    FJ423446 Genomic DNA. Translation: ACJ50155.1 . Different initiation.
    BK000554 Genomic DNA. Translation: DAA00968.1 .
    PIRi T08024.
    RefSeqi NP_958424.2. NC_005353.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Q90 X-ray 3.10 L 12-43 [» ]
    ProteinModelPortali P50369.
    SMRi P50369. Positions 12-43.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi DAA00968 ; DAA00968 ; DAA00968 .
    GeneIDi 2717047.
    KEGGi cre:ChreCp068.

    Enzyme and pathway databases

    BioCyci CHLAMY:CHRECP068-MONOMER.
    MetaCyc:CHRECP068-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P50369.

    Family and domain databases

    HAMAPi MF_00433. Cytb6_f_PetL.
    InterProi IPR007802. Cyt_b6/f_cplx_su6.
    [Graphical view ]
    Pfami PF05115. PetL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The chloroplast ycf7 (petL) open reading frame of Chlamydomonas reinhardtii encodes a small functionally important subunit of the cytochrome b6f complex."
      Takahashi Y., Rahire M., Breyton C., Popot J.-L., Joliot P., Rochaix J.-D.
      EMBO J. 15:3498-3506(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 137c / CC-125.
    2. "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome: addressing the mutational-hazard hypothesis."
      Smith D.R., Lee R.W.
      BMC Evol. Biol. 9:120-120(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CC-503.
    3. "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats."
      Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H., Stern D.B.
      Plant Cell 14:2659-2679(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, COMPLETE PLASTID GENOME.
    4. "Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii."
      Pierre Y., Breyton C., Kramer D., Popot J.-L.
      J. Biol. Chem. 270:29342-29349(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: WT12.
    5. "Chimeric fusions of subunit IV and PetL in the b6f complex of Chlamydomonas reinhardtii: structural implications and consequences on state transitions."
      Zito F., Vinh J., Popot J.-L., Finazzi G.
      J. Biol. Chem. 277:12446-12455(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF START CODON, MASS SPECTROMETRY, FUSION WITH SUBUNIT IV (PETD).
      Strain: 137c / CC-125.
    6. "An atypical haem in the cytochrome b(6)f complex."
      Stroebel D., Choquet Y., Popot J.-L., Picot D.
      Nature 426:413-418(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 12-43.

    Entry informationi

    Entry nameiPETL_CHLRE
    AccessioniPrimary (citable) accession number: P50369
    Secondary accession number(s): B7U1K8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 11, 2003
    Last modified: October 1, 2014
    This is version 79 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    A deletion of this gene can be substituted for by a subunit IV-petL fusion. The fusion protein does not permit state transitions, although all other functions are wild-type.

    Caution

    Mass spectrometry indicates that the start site is probably Met-1, and not Met-12 as is thought to be the case in other organisms.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3