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P50369

- PETL_CHLRE

UniProt

P50369 - PETL_CHLRE

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Protein
Cytochrome b6-f complex subunit 6
Gene
petL
Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. PetL is important for photoautotrophic growth as well as for electron transfer efficiency and stability of the cytochrome b6-f complex By similarity.UniRule annotation

GO - Molecular functioni

  1. electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB-HAMAP
  2. photosynthesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Enzyme and pathway databases

BioCyciCHLAMY:CHRECP068-MONOMER.
MetaCyc:CHRECP068-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b6-f complex subunit 6
Alternative name(s):
Cytochrome b6-f complex subunit PetL
Cytochrome b6-f complex subunit VI
Gene namesi
Name:petL
Encoded oniPlastid; Chloroplast
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas
ProteomesiUP000006906: Chloroplast

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei17 – 3721Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. chloroplast thylakoid membrane Source: UniProtKB-SubCell
  2. cytochrome b6f complex Source: InterPro
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4343Cytochrome b6-f complex subunit 6UniRule annotation
PRO_0000220445Add
BLAST

Post-translational modificationi

The N-terminus is blocked.UniRule annotation

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer By similarity.1 Publication

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 3725

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q90X-ray3.10L12-43[»]
ProteinModelPortaliP50369.
SMRiP50369. Positions 12-43.

Miscellaneous databases

EvolutionaryTraceiP50369.

Family & Domainsi

Sequence similaritiesi

Belongs to the PetL family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

HAMAPiMF_00433. Cytb6_f_PetL.
InterProiIPR007802. Cyt_b6/f_cplx_su6.
[Graphical view]
PfamiPF05115. PetL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50369-1 [UniParc]FASTAAdd to Basket

« Hide

MIFDFNYIHI FMLTITSYVG LLIGALVFTL GIYLGLLKVV KLI          43
Length:43
Mass (Da):4,878
Last modified:July 11, 2003 - v3
Checksum:iB7E6977ED788C9F3
GO

Sequence cautioni

The sequence ACJ50155.1 differs from that shown. Reason: Erroneous initiation.

Mass spectrometryi

Molecular mass is 3467 Da from positions 1 - 30. Determined by MALDI. 1 Publication
Molecular mass is 3918 Da from positions 5 - 39. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U43964 Genomic DNA. Translation: AAB17716.1.
FJ423446 Genomic DNA. Translation: ACJ50155.1. Different initiation.
BK000554 Genomic DNA. Translation: DAA00968.1.
PIRiT08024.
RefSeqiNP_958424.2. NC_005353.1.

Genome annotation databases

EnsemblPlantsiDAA00968; DAA00968; DAA00968.
GeneIDi2717047.
KEGGicre:ChreCp068.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U43964 Genomic DNA. Translation: AAB17716.1 .
FJ423446 Genomic DNA. Translation: ACJ50155.1 . Different initiation.
BK000554 Genomic DNA. Translation: DAA00968.1 .
PIRi T08024.
RefSeqi NP_958424.2. NC_005353.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Q90 X-ray 3.10 L 12-43 [» ]
ProteinModelPortali P50369.
SMRi P50369. Positions 12-43.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi DAA00968 ; DAA00968 ; DAA00968 .
GeneIDi 2717047.
KEGGi cre:ChreCp068.

Enzyme and pathway databases

BioCyci CHLAMY:CHRECP068-MONOMER.
MetaCyc:CHRECP068-MONOMER.

Miscellaneous databases

EvolutionaryTracei P50369.

Family and domain databases

HAMAPi MF_00433. Cytb6_f_PetL.
InterProi IPR007802. Cyt_b6/f_cplx_su6.
[Graphical view ]
Pfami PF05115. PetL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The chloroplast ycf7 (petL) open reading frame of Chlamydomonas reinhardtii encodes a small functionally important subunit of the cytochrome b6f complex."
    Takahashi Y., Rahire M., Breyton C., Popot J.-L., Joliot P., Rochaix J.-D.
    EMBO J. 15:3498-3506(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 137c / CC-125.
  2. "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome: addressing the mutational-hazard hypothesis."
    Smith D.R., Lee R.W.
    BMC Evol. Biol. 9:120-120(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CC-503.
  3. "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats."
    Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H., Stern D.B.
    Plant Cell 14:2659-2679(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, COMPLETE PLASTID GENOME.
  4. "Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii."
    Pierre Y., Breyton C., Kramer D., Popot J.-L.
    J. Biol. Chem. 270:29342-29349(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: WT12.
  5. "Chimeric fusions of subunit IV and PetL in the b6f complex of Chlamydomonas reinhardtii: structural implications and consequences on state transitions."
    Zito F., Vinh J., Popot J.-L., Finazzi G.
    J. Biol. Chem. 277:12446-12455(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF START CODON, MASS SPECTROMETRY, FUSION WITH SUBUNIT IV (PETD).
    Strain: 137c / CC-125.
  6. "An atypical haem in the cytochrome b(6)f complex."
    Stroebel D., Choquet Y., Popot J.-L., Picot D.
    Nature 426:413-418(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 12-43.

Entry informationi

Entry nameiPETL_CHLRE
AccessioniPrimary (citable) accession number: P50369
Secondary accession number(s): B7U1K8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 11, 2003
Last modified: May 14, 2014
This is version 78 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

A deletion of this gene can be substituted for by a subunit IV-petL fusion. The fusion protein does not permit state transitions, although all other functions are wild-type.

Caution

Mass spectrometry indicates that the start site is probably Met-1, and not Met-12 as is thought to be the case in other organisms.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3