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P50369 (PETL_CHLRE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytochrome b6-f complex subunit 6
Alternative name(s):
Cytochrome b6-f complex subunit PetL
Cytochrome b6-f complex subunit VI
Gene names
Name:petL
Encoded onPlastid; Chloroplast
OrganismChlamydomonas reinhardtii (Chlamydomonas smithii) [Reference proteome]
Taxonomic identifier3055 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Protein attributes

Sequence length43 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. PetL is important for photoautotrophic growth as well as for electron transfer efficiency and stability of the cytochrome b6-f complex By similarity. HAMAP-Rule MF_00433

Subunit structure

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer By similarity. Ref.5

Subcellular location

Plastidchloroplast thylakoid membrane; Single-pass membrane protein By similarity HAMAP-Rule MF_00433.

Post-translational modification

The N-terminus is blocked. HAMAP-Rule MF_00433

Miscellaneous

A deletion of this gene can be substituted for by a subunit IV-petL fusion. The fusion protein does not permit state transitions, although all other functions are wild-type.

Sequence similarities

Belongs to the PetL family.

Caution

Mass spectrometry indicates that the start site is probably Met-1, and not Met-12 as is thought to be the case in other organisms.

Mass spectrometry

Molecular mass is 3467 Da from positions 1 - 30. Determined by MALDI. Ref.5

Molecular mass is 3918 Da from positions 5 - 39. Determined by MALDI. Ref.5

Sequence caution

The sequence ACJ50155.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4343Cytochrome b6-f complex subunit 6 HAMAP-Rule MF_00433
PRO_0000220445

Regions

Transmembrane17 – 3721Helical; Potential

Secondary structure

... 43
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P50369 [UniParc].

Last modified July 11, 2003. Version 3.
Checksum: B7E6977ED788C9F3

FASTA434,878
        10         20         30         40 
MIFDFNYIHI FMLTITSYVG LLIGALVFTL GIYLGLLKVV KLI 

« Hide

References

« Hide 'large scale' references
[1]"The chloroplast ycf7 (petL) open reading frame of Chlamydomonas reinhardtii encodes a small functionally important subunit of the cytochrome b6f complex."
Takahashi Y., Rahire M., Breyton C., Popot J.-L., Joliot P., Rochaix J.-D.
EMBO J. 15:3498-3506(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 137c / CC-125.
[2]"Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome: addressing the mutational-hazard hypothesis."
Smith D.R., Lee R.W.
BMC Evol. Biol. 9:120-120(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CC-503.
[3]"The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats."
Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H., Stern D.B.
Plant Cell 14:2659-2679(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, COMPLETE PLASTID GENOME.
[4]"Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii."
Pierre Y., Breyton C., Kramer D., Popot J.-L.
J. Biol. Chem. 270:29342-29349(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: WT12.
[5]"Chimeric fusions of subunit IV and PetL in the b6f complex of Chlamydomonas reinhardtii: structural implications and consequences on state transitions."
Zito F., Vinh J., Popot J.-L., Finazzi G.
J. Biol. Chem. 277:12446-12455(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF START CODON, MASS SPECTROMETRY, FUSION WITH SUBUNIT IV (PETD).
Strain: 137c / CC-125.
[6]"An atypical haem in the cytochrome b(6)f complex."
Stroebel D., Choquet Y., Popot J.-L., Picot D.
Nature 426:413-418(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 12-43.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43964 Genomic DNA. Translation: AAB17716.1.
FJ423446 Genomic DNA. Translation: ACJ50155.1. Different initiation.
BK000554 Genomic DNA. Translation: DAA00968.1.
PIRT08024.
RefSeqNP_958424.2. NC_005353.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q90X-ray3.10L12-43[»]
ProteinModelPortalP50369.
SMRP50369. Positions 12-43.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsDAA00968; DAA00968; DAA00968.
GeneID2717047.
KEGGcre:ChreCp068.

Phylogenomic databases

ProtClustDBCHL00106.

Enzyme and pathway databases

BioCycCHLAMY:CHRECP068-MONOMER.
MetaCyc:CHRECP068-MONOMER.

Family and domain databases

HAMAPMF_00433. Cytb6_f_PetL.
InterProIPR007802. Cyt_b6/f_cplx_su6.
[Graphical view]
PfamPF05115. PetL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP50369.

Entry information

Entry namePETL_CHLRE
AccessionPrimary (citable) accession number: P50369
Secondary accession number(s): B7U1K8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 11, 2003
Last modified: February 19, 2014
This is version 77 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references