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Protein

Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic

Gene

GAPA

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711NADPBy similarity
Binding sitei116 – 1161NADP; via carbonyl oxygenBy similarity
Active sitei190 – 1901NucleophilePROSITE-ProRule annotation
Sitei217 – 2171Activates thiol group during catalysisBy similarity
Binding sitei220 – 2201Glyceraldehyde 3-phosphateBy similarity
Binding sitei235 – 2351Glyceraldehyde 3-phosphateBy similarity
Binding sitei271 – 2711Glyceraldehyde 3-phosphateBy similarity
Binding sitei353 – 3531NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi47 – 482NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Calvin cycle

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic (EC:1.2.1.13)
Alternative name(s):
NADP-dependent glyceraldehydephosphate dehydrogenase subunit A
Gene namesi
Name:GAPA
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434ChloroplastBy similarityAdd
BLAST
Chaini35 – 374340Glyceraldehyde-3-phosphate dehydrogenase A, chloroplasticPRO_0000010418Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi55 ↔ 325By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP50362.
PRIDEiP50362.
ProMEXiP50362.

Interactioni

Subunit structurei

Homotetramer. Component of a complex that contains two dimers of PRK, two tetramers of GAPDH and CP12. CP12 associates with GAPDH, causing its conformation to change. This GAPDH/CP12 complex binds PRK to form a half-complex (one unit). This unit probably dimerizes due partially to interactions between the enzymes of each unit.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CP12A6Q0K52EBI-9538536,EBI-9538486

Protein-protein interaction databases

IntActiP50362. 1 interaction.
STRINGi3055.EDP09609.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NLGmodel-A35-374[»]
1NLHmodel-A35-374[»]
ProteinModelPortaliP50362.
SMRiP50362. Positions 37-374.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 1913Glyceraldehyde 3-phosphate bindingBy similarity
Regioni248 – 2492Glyceraldehyde 3-phosphate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
KOiK05298.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50362-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAMMQKSAF TGSAVSSKSG VRAKAARAVV DVRAEKKIRV AINGFGRIGR
60 70 80 90 100
NFLRCWHGRQ NTLLDVVAIN DSGGVKQASH LLKYDSTLGT FAADVKIVDD
110 120 130 140 150
SHISVDGKQI KIVSSRDPLQ LPWKEMNIDL VIEGTGVFID KVGAGKHIQA
160 170 180 190 200
GASKVLITAP AKDKDIPTFV VGVNEGDYKH EYPIISNASC TTNCLAPFVK
210 220 230 240 250
VLEQKFGIVK GTMTTTHSYT GDQRLLDASH RDLRRARAAA LNIVPTTTGA
260 270 280 290 300
AKAVSLVLPS LKGKLNGIAL RVPTPTVSVV DLVVQVEKKT FAEEVNAAFR
310 320 330 340 350
EAANGPMKGV LHVEDAPLVS IDFKCTDQST SIDASLTMVM GDDMVKVVAW
360 370
YDNEWGYSQR VVDLAEVTAK KWVA
Length:374
Mass (Da):40,304
Last modified:October 1, 1996 - v1
Checksum:i8CEB02897930D34C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27668 Genomic DNA. Translation: AAA86855.1.
PIRiT07990.
RefSeqiXP_001689871.1. XM_001689819.1.
UniGeneiCre.2829.

Genome annotation databases

EnsemblPlantsiEDP09609; EDP09609; CHLREDRAFT_129019.
GeneIDi5715019.
GrameneiEDP09609; EDP09609; CHLREDRAFT_129019.
KEGGicre:CHLREDRAFT_129019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27668 Genomic DNA. Translation: AAA86855.1.
PIRiT07990.
RefSeqiXP_001689871.1. XM_001689819.1.
UniGeneiCre.2829.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NLGmodel-A35-374[»]
1NLHmodel-A35-374[»]
ProteinModelPortaliP50362.
SMRiP50362. Positions 37-374.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP50362. 1 interaction.
STRINGi3055.EDP09609.

Proteomic databases

PaxDbiP50362.
PRIDEiP50362.
ProMEXiP50362.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiEDP09609; EDP09609; CHLREDRAFT_129019.
GeneIDi5715019.
GrameneiEDP09609; EDP09609; CHLREDRAFT_129019.
KEGGicre:CHLREDRAFT_129019.

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
KOiK05298.

Enzyme and pathway databases

UniPathwayiUPA00116.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3PA_CHLRE
AccessioniPrimary (citable) accession number: P50362
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 17, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

GAPDHB, the second subunit found in plants, is absent in algae.
Algae contain three forms of GAPDH: two cytosolic forms which participate in glycolysis and one chloroplastic form which participates in photosynthesis. These three forms are encoded by distinct genes.

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.