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Protein

Tryptase beta-2

Gene

Tpsb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Plays a role in innate immunity.By similarity

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73Charge relay systemBy similarity1
Active sitei120Charge relay systemBy similarity1
Active sitei223Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.025.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptase beta-2 (EC:3.4.21.59)
Short name:
Tryptase-2
Alternative name(s):
Mast cell protease 6
Short name:
rMCP-6
Gene namesi
Name:Tpsb2
Synonyms:Mcp6, Mcpt6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3065. Tpsb2.

Subcellular locationi

  • Secreted By similarity

  • Note: Released from the secretory granules upon mast cell activation.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000002749220 – 29Activation peptide10
ChainiPRO_000002749330 – 274Tryptase beta-2Add BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi58 ↔ 74PROSITE-ProRule annotation
Modified residuei96PhosphotyrosineBy similarity1
Glycosylationi104N-linked (GlcNAc...)Sequence analysis1
Glycosylationi131N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi154 ↔ 229PROSITE-ProRule annotation
Disulfide bondi187 ↔ 210PROSITE-ProRule annotation
Disulfide bondi219 ↔ 247PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen

Proteomic databases

PaxDbiP50343.
PRIDEiP50343.

PTM databases

PhosphoSitePlusiP50343.

Interactioni

Subunit structurei

Homotetramer. The active tetramer is converted to inactive monomers at neutral and acidic pH in the absence of heparin. Low concentrations of inactive monomers become active monomers at pH 6.0 in the presence of heparin. When the concentration of active monomers is higher, they convert to active monomers and then to active tetramers. These monomers are active and functionally distinct from the tetrameric enzyme. In contrast to the hidden active sites in the tetrameric form, the active site of the monomeric form is accessible for macromolecular proteins and inhibitors eg: fibrinogen which is a substrate for the monomeric but not for the tetrameric form. The monomeric form forms a complex with SERPINB6.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025220.

Structurei

3D structure databases

ProteinModelPortaliP50343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 271Peptidase S1PROSITE-ProRule annotationAdd BLAST242

Sequence similaritiesi

Belongs to the peptidase S1 family. Tryptase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP50343.
KOiK01340.
PhylomeDBiP50343.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50343-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKLLLLLAL SPLASLVHAA PCPVKQRVGI VGGREASESK WPWQVSLRFK
60 70 80 90 100
FSFWMHFCGG SLIHPQWVLT AAHCVGLHIK SPELFRVQLR EQYLYYADQL
110 120 130 140 150
LTVNRTVVHP HYYTVEDGAD IALLELEIPV NVSTHIHPIS LPPASETFPS
160 170 180 190 200
GTSCWVTGWG DIDSDEPLLP PYPLKQVKVP IVENSLCDRK YHTGLYTGDD
210 220 230 240 250
VPIVQDGMLC AGNTRSDSCQ GDSGGPLVCK VKGTWLQAGV VSWGEGCAEA
260 270
NRPGIYTRVT YYLDWIHRYV PQRS
Length:274
Mass (Da):30,508
Last modified:October 1, 1996 - v1
Checksum:iDF84D55668CA1A25
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti128I → N in AAB48262 (PubMed:8996238).Curated1
Sequence conflicti139I → T in AAB48262 (PubMed:8996238).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38455 mRNA. Translation: BAA07486.1.
U67909 mRNA. Translation: AAB48262.1.
PIRiJC4171.
RefSeqiNP_062053.2. NM_019180.2.
UniGeneiRn.10183.

Genome annotation databases

GeneIDi29268.
KEGGirno:29268.
UCSCiRGD:3065. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38455 mRNA. Translation: BAA07486.1.
U67909 mRNA. Translation: AAB48262.1.
PIRiJC4171.
RefSeqiNP_062053.2. NM_019180.2.
UniGeneiRn.10183.

3D structure databases

ProteinModelPortaliP50343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025220.

Protein family/group databases

MEROPSiS01.025.

PTM databases

PhosphoSitePlusiP50343.

Proteomic databases

PaxDbiP50343.
PRIDEiP50343.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29268.
KEGGirno:29268.
UCSCiRGD:3065. rat.

Organism-specific databases

CTDi64499.
RGDi3065. Tpsb2.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP50343.
KOiK01340.
PhylomeDBiP50343.

Miscellaneous databases

PROiP50343.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRYB2_RAT
AccessioniPrimary (citable) accession number: P50343
Secondary accession number(s): P97593
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.