Reviewed, UniProtKB/Swiss-Prot P50343 (TRYB2_RAT)

Names and origin

Protein names

Recommended name:
    Tryptase beta-2
      Short name=Tryptase-2
    EC=3.4.21.59
Alternative name(s):
    Mast cell protease 6
      Short name=rMCP-6

Gene names

Name:	Tpsb2
Synonyms:	Mcp6, Mcpt6

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection By similarity.
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.
Subunit structure	Homotetramer By similarity.
Subcellular location	Secreted By similarity. Note: Released from the secretory granules upon mast cell activation By similarity.
Sequence similarities	Belongs to the peptidase S1 family. Tryptase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Phosphoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 29	10	Activation peptide		PRO_0000027492
	Chain	30 – 274	245	Tryptase beta-2		PRO_0000027493
Regions
	Domain	30 – 271	242	Peptidase S1
Sites
	Active site	73	1	Charge relay system By similarity
	Active site	120	1	Charge relay system By similarity
	Active site	223	1	Charge relay system By similarity
Amino acid modifications
	Modified residue	96	1	Phosphotyrosine By similarity
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
	Glycosylation	131	1	N-linked (GlcNAc...) Potential
	Disulfide bond	58 ↔ 74		By similarity
	Disulfide bond	154 ↔ 229		By similarity
	Disulfide bond	187 ↔ 210		By similarity
	Disulfide bond	219 ↔ 247		By similarity
Experimental info
	Sequence conflict	128	1	I → N in AAB48262. Ref.2
	Sequence conflict	139	1	I → T in AAB48262. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P50343-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: DF84D55668CA1A25	FASTA	274	30,508
	10 20 30 40 50 60 MLKLLLLLAL SPLASLVHAA PCPVKQRVGI VGGREASESK WPWQVSLRFK FSFWMHFCGG 70 80 90 100 110 120 SLIHPQWVLT AAHCVGLHIK SPELFRVQLR EQYLYYADQL LTVNRTVVHP HYYTVEDGAD 130 140 150 160 170 180 IALLELEIPV NVSTHIHPIS LPPASETFPS GTSCWVTGWG DIDSDEPLLP PYPLKQVKVP 190 200 210 220 230 240 IVENSLCDRK YHTGLYTGDD VPIVQDGMLC AGNTRSDSCQ GDSGGPLVCK VKGTWLQAGV 250 260 270 VSWGEGCAEA NRPGIYTRVT YYLDWIHRYV PQRS

References

[1]	"cDNA sequencing and expression of rat mast cell tryptase." Ide H., Itoh H., Tomita M., Murakumo Y., Kobayashi T., Maruyama H., Osada Y., Nawa Y. J. Biochem. 118:210-215(1995) [PubMed: 8537314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Peritoneal mast cell.
[2]	"Secretory granule proteases in rat mast cells. Cloning of 10 different serine proteases and a carboxypeptidase A from various rat mast cell populations." Lutzelschwab C., Pejler G., Aveskogh M., Hellman L. J. Exp. Med. 185:13-29(1997) [PubMed: 8996238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Peritoneal mast cell.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D38455 mRNA. Translation: BAA07486.1. U67909 mRNA. Translation: AAB48262.1.
IPI	IPI00197426.
PIR	JC4171.
RefSeq	NP_062053.1.
UniGene	Rn.10183
3D structure databases
HSSP	HSSP built from PDB template 1A0L based on UniProtKB P20231.
SMR	P50343. Positions 30-272.
ModBase	Search...
Protein family/group databases
MEROPS	S01.025.
PTM databases
PhosphoSite	P50343.
Genome annotation databases
Ensembl	ENSRNOT00000025220; ENSRNOP00000025220; ENSRNOG00000018611; Rattus norvegicus. [Genome view]
GeneID	29268.
KEGG	rno:29268.
UCSC	NM_019180. rat.
Organism-specific databases
CTD	29268.
RGD	3065. Tpsb2.
Phylogenomic databases
HOVERGEN	P50343.
Enzyme and pathway databases
BRENDA	3.4.21.59. 248.
Gene expression databases
ArrayExpress	P50343.
Genevestigator	P50343.
GermOnline	ENSRNOG00000018611. Rattus norvegicus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	608608.

Entry information

Entry name

TRYB2_RAT

Accession

Primary (citable) accession number: P50343
Secondary accession number(s): P97593

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	October 13, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)