P50342 (TRYT_MERUN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Mast cell tryptase EC=3.4.21.59
Organism	Meriones unguiculatus (Mongolian jird) (Mongolian gerbil)
Taxonomic identifier	10047 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Gerbillinae › Meriones

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.
Sequence similarities	Belongs to the peptidase S1 family. Tryptase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 270	245	Mast cell tryptase		PRO_0000027485
Regions
	Domain	26 – 267	242	Peptidase S1
Sites
	Active site	69	1	Charge relay system By similarity
	Active site	116	1	Charge relay system By similarity
	Active site	219	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	46	1	N-linked (GlcNAc...) Potential
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	228	1	N-linked (GlcNAc...) Potential
	Disulfide bond	54 ↔ 70		By similarity
	Disulfide bond	150 ↔ 225		By similarity
	Disulfide bond	183 ↔ 206		By similarity
	Disulfide bond	215 ↔ 243		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50342 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 1BE102DB86943401	FASTA	270	30,167
	10 20 30 40 50 60 MLKLLLLALP LFSLMHRSPL CQEWGIVGGQ EAPGNKWPWQ VSLRANETYW RHFCGGSLIH 70 80 90 100 110 120 PQWVLTAAHC VGPTIADPNK VRVQLRKQYL YYHDHLLAVS RIITHPTFYA TQNGADIALL 130 140 150 160 170 180 ELKNPVNISS HVHPVSLPPA SETFPSGTLC WVTGWGNIDN DVSLPPPFPL KEVQVPVVEN 190 200 210 220 230 240 QLCDLKYHKG VYTGDNIHIV RDDMLCAGNE GHDSCQGDSG GPLVCKVNGT WLQAGVVSWG 250 260 270 EGCALPNRPG IYTRVTYYLD WIHRYVPKDF

References

[1]	"Cloning of the cDNA encoding mast cell tryptase of Mongolian gerbil, Meriones unguiculatus, and its preferential expression in the intestinal mucosa." Murakumo Y., Ide H., Itoh H., Tomita M., Kobayashi T., Maruyama H., Horii Y., Nawa Y. Biochem. J. 309:921-926(1995) [PubMed: 7639711] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: MGS/SEA. Tissue: Intestine.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D31789 mRNA. Translation: BAA06598.1.
PIR	S56160.
3D structure databases
ProteinModelPortal	P50342.
SMR	P50342. Positions 26-268.
ModBase	Search...
Protein family/group databases
MEROPS	S01.026.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG013304.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYT_MERUN

Accession

Primary (citable) accession number: P50342

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 16, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program