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P50339

- CMA1_RAT

UniProt

P50339 - CMA1_RAT

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Protein

Chymase

Gene

Cma1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.By similarity

Catalytic activityi

Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Charge relay systemBy similarity
Active sitei110 – 1101Charge relay systemBy similarity
Active sitei203 – 2031Charge relay systemBy similarity

GO - Molecular functioni

  1. peptidase activity Source: RGD
  2. peptide binding Source: RGD
  3. serine-type endopeptidase activity Source: RGD

GO - Biological processi

  1. cellular response to glucose stimulus Source: RGD
  2. interleukin-1 beta biosynthetic process Source: Ensembl
  3. midbrain development Source: RGD
  4. peptide metabolic process Source: RGD
  5. positive regulation of angiogenesis Source: RGD
  6. proteolysis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiREACT_195025. Signaling by SCF-KIT.
REACT_198590. Activation of Matrix Metalloproteinases.
REACT_198807. Metabolism of Angiotensinogen to Angiotensins.

Protein family/group databases

MEROPSiS01.150.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymase (EC:3.4.21.39)
Alternative name(s):
Alpha-chymase
Mast cell protease 3
Short name:
rMCP-3
Mast cell protease 5
Short name:
rMCP-5
Mast cell protease III
Short name:
rMCP-III
Gene namesi
Name:Cma1
Synonyms:Mcpt3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 15

Organism-specific databases

RGDi2365. Cma1.

Subcellular locationi

Secreted. Cytoplasmic granule
Note: Secretory granules.

GO - Cellular componenti

  1. extracellular matrix Source: Ensembl
  2. extracellular region Source: RGD
  3. intracellular Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Propeptidei20 – 212Activation peptidePRO_0000027443
Chaini22 – 247226ChymasePRO_0000027444Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi51 ↔ 67PROSITE-ProRule annotation
Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi144 ↔ 209PROSITE-ProRule annotation
Disulfide bondi175 ↔ 188PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiP50339.

Expressioni

Tissue specificityi

Mast cells.

Gene expression databases

GenevestigatoriP50339.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliP50339.
SMRiP50339. Positions 22-247.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 245224Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00760000118895.
HOVERGENiHBG013304.
InParanoidiP50339.
KOiK01329.
OMAiTLHHDIM.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP50339.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50339-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNLHALCLLL LLLGSSTKAG EIIGGTECIP HSRPYMAYLE IVTSDNYLSA
60 70 80 90 100
CSGFLIRRNF VLTAAHCAGR SITVLLGAHN KTYKEDTWQK LEVEKQFIHP
110 120 130 140 150
NYDKRLVLHD IMLLKLKEKA KLTLGVGTLP LSANFNFIPP GRMCRAVGWG
160 170 180 190 200
RTNVNEPASD TLQEVKMRLQ EPQSCKHFTS FQHKSQLCVG NPKKMQNVYK
210 220 230 240
GDSGGPLLCA GIAQGIASYV HPNAKPPAVF TRISHYRPWI NKILREN
Length:247
Mass (Da):27,569
Last modified:October 1, 1996 - v1
Checksum:i6525D7BF1BFDF053
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38495 mRNA. Translation: BAA07507.1.
U67908 mRNA. Translation: AAB48261.1.
PIRiS59135.
RefSeqiNP_037224.1. NM_013092.1.
UniGeneiRn.10182.

Genome annotation databases

EnsembliENSRNOT00000067539; ENSRNOP00000061330; ENSRNOG00000020563.
GeneIDi25627.
KEGGirno:25627.
UCSCiRGD:2365. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38495 mRNA. Translation: BAA07507.1 .
U67908 mRNA. Translation: AAB48261.1 .
PIRi S59135.
RefSeqi NP_037224.1. NM_013092.1.
UniGenei Rn.10182.

3D structure databases

ProteinModelPortali P50339.
SMRi P50339. Positions 22-247.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P50339.
ChEMBLi CHEMBL3168.

Protein family/group databases

MEROPSi S01.150.

Proteomic databases

PRIDEi P50339.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000067539 ; ENSRNOP00000061330 ; ENSRNOG00000020563 .
GeneIDi 25627.
KEGGi rno:25627.
UCSCi RGD:2365. rat.

Organism-specific databases

CTDi 1215.
RGDi 2365. Cma1.

Phylogenomic databases

GeneTreei ENSGT00760000118895.
HOVERGENi HBG013304.
InParanoidi P50339.
KOi K01329.
OMAi TLHHDIM.
OrthoDBi EOG7RRF7Z.
PhylomeDBi P50339.

Enzyme and pathway databases

Reactomei REACT_195025. Signaling by SCF-KIT.
REACT_198590. Activation of Matrix Metalloproteinases.
REACT_198807. Metabolism of Angiotensinogen to Angiotensins.

Miscellaneous databases

NextBioi 607417.

Gene expression databases

Genevestigatori P50339.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of the cDNA encoding a novel rat mast-cell proteinase, rMCP-3, and its expression in comparison with other rat mast-cell proteinases."
    Ide H., Itoh H., Tomita M., Murakumo Y., Kobayashi T., Maruyama H., Osada Y., Nawa Y.
    Biochem. J. 311:675-680(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Peritoneal mast cell.
  2. "Secretory granule proteases in rat mast cells. Cloning of 10 different serine proteases and a carboxypeptidase A from various rat mast cell populations."
    Lutzelschwab C., Pejler G., Aveskogh M., Hellman L.
    J. Exp. Med. 185:13-29(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-247.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiCMA1_RAT
AccessioniPrimary (citable) accession number: P50339
Secondary accession number(s): Q9R2C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3