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P50320 (PGKP_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase, plasmid

EC=2.7.2.3
Gene names
Name:cbbKP
Ordered Locus Names:PHG417
Encoded onPlasmid megaplasmid pHG1
OrganismCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate biosynthesis; Calvin cycle. HAMAP-Rule MF_00145

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00145

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processCalvin cycle
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Plasmid
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

reductive pentose-phosphate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412Phosphoglycerate kinase, plasmid HAMAP-Rule MF_00145
PRO_0000145896

Regions

Nucleotide binding365 – 3684ATP By similarity
Region39 – 413Substrate binding By similarity
Region78 – 814Substrate binding By similarity

Sites

Binding site551Substrate By similarity
Binding site1331Substrate By similarity
Binding site1661Substrate By similarity
Binding site2171ATP By similarity
Binding site3391ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P50320 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 9E84C666859E7274

FASTA41242,299
        10         20         30         40         50         60 
MMSLSHASVP HTNPTAPHTL AALLAAGGLA GKRVFIRADL NVPQDAAGDI TDDTRIRASV 

        70         80         90        100        110        120 
PAIAACLQAG AAVMVTSHLG RPQEGAPDPR HSLAPVGRRL SELLGRQVPL LSGWTEGGFQ 

       130        140        150        160        170        180 
VPPGQVVLLE NCRMNTGEKK NSDELAQKMA ALCDVYVNDA FGTAHRAEAT THGIARYAPV 

       190        200        210        220        230        240 
ACAGPLLAAE IDALGKALGQ PARPLVAIVA GSKVSTKLTI LKSLADKVDN LVVGGGIANT 

       250        260        270        280        290        300 
FMLAAGLKIG KSLAEPDLLA DARAIIDIMA ARGASVPIPV DVVCAKEFSA TAAAAVKDVR 

       310        320        330        340        350        360 
DVADDDMILD IGPKTAAMLA DQLKAAGTIV WNGPVGVFEF DQFGNGTRVL AQAIAESKAF 

       370        380        390        400        410 
SIAGGGDTLA AIAKYGIADR VGYISTGGGA FLEFLEGKKL PALDVLEQRA AS 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the genes forming the distal parts of the two cbb CO2 fixation operons from Alcaligenes eutrophus."
Schaeferfohann J., Yoo J.-G., Bowien B.
Arch. Microbiol. 163:291-299(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16 megaplasmid encoding key enzymes of H(2)-based lithoautotrophy and anaerobiosis."
Schwartz E., Henne A., Cramm R., Eitinger T., Friedrich B., Gottschalk G.
J. Mol. Biol. 332:369-383(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12423 Genomic DNA. Translation: AAC43447.1.
AY305378 Genomic DNA. Translation: AAP86166.1.
PIRI39554.
RefSeqNP_943052.1. NC_005241.1.

3D structure databases

ProteinModelPortalP50320.
SMRP50320. Positions 29-410.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381666.PHG417.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2656761.
KEGGreh:PHG417.
PATRIC35229346. VBIRalEut6770_0345.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0126.
HOGENOMHOG000227107.
KOK00927.
OMAYKEYITH.
OrthoDBEOG64N9Z0.
ProtClustDBPRK00073.

Enzyme and pathway databases

BioCycCNEC381666:GJUJ-6691-MONOMER.
UniPathwayUPA00116.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGKP_CUPNH
AccessionPrimary (citable) accession number: P50320
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways