P50320 (PGKP_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase, plasmid
EC=2.7.2.3

Gene names

Name:	cbbKP
Ordered Locus Names:	PHG417

Encoded on

Plasmid megaplasmid pHG1

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus necator

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145
Pathway	Carbohydrate biosynthesis; Calvin cycle. HAMAP-Rule MF_00145
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00145.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Phosphoglycerate kinase, plasmid HAMAP-Rule MF_00145		PRO_0000145896
Regions
	Nucleotide binding	365 – 368	4	ATP By similarity
	Region	39 – 41	3	Substrate binding By similarity
	Region	78 – 81	4	Substrate binding By similarity
Sites
	Binding site	55	1	Substrate By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	217	1	ATP By similarity
	Binding site	339	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50320 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 9E84C666859E7274	FASTA	412	42,299
	10 20 30 40 50 60 MMSLSHASVP HTNPTAPHTL AALLAAGGLA GKRVFIRADL NVPQDAAGDI TDDTRIRASV 70 80 90 100 110 120 PAIAACLQAG AAVMVTSHLG RPQEGAPDPR HSLAPVGRRL SELLGRQVPL LSGWTEGGFQ 130 140 150 160 170 180 VPPGQVVLLE NCRMNTGEKK NSDELAQKMA ALCDVYVNDA FGTAHRAEAT THGIARYAPV 190 200 210 220 230 240 ACAGPLLAAE IDALGKALGQ PARPLVAIVA GSKVSTKLTI LKSLADKVDN LVVGGGIANT 250 260 270 280 290 300 FMLAAGLKIG KSLAEPDLLA DARAIIDIMA ARGASVPIPV DVVCAKEFSA TAAAAVKDVR 310 320 330 340 350 360 DVADDDMILD IGPKTAAMLA DQLKAAGTIV WNGPVGVFEF DQFGNGTRVL AQAIAESKAF 370 380 390 400 410 SIAGGGDTLA AIAKYGIADR VGYISTGGGA FLEFLEGKKL PALDVLEQRA AS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the genes forming the distal parts of the two cbb CO2 fixation operons from Alcaligenes eutrophus." Schaeferfohann J., Yoo J.-G., Bowien B. Arch. Microbiol. 163:291-299(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16 megaplasmid encoding key enzymes of H(2)-based lithoautotrophy and anaerobiosis." Schwartz E., Henne A., Cramm R., Eitinger T., Friedrich B., Gottschalk G. J. Mol. Biol. 332:369-383(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12423 Genomic DNA. Translation: AAC43447.1. AY305378 Genomic DNA. Translation: AAP86166.1.
PIR	I39554.
RefSeq	NP_943052.1. NC_005241.1.
3D structure databases
ProteinModelPortal	P50320.
SMR	P50320. Positions 29-410.
ModBase	Search...
Protein-protein interaction databases
STRING	381666.PHG417.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2656761.
KEGG	reh:PHG417.
PATRIC	35229346. VBIRalEut6770_0345.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0126.
HOGENOM	HOG000227107.
KO	K00927.
OMA	ANKNNCK.
ProtClustDB	PRK00073.
Enzyme and pathway databases
BioCyc	CNEC381666:GJUJ-6691-MONOMER.
UniPathway	UPA00116.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
HAMAP	MF_00145. Phosphoglyc_kinase.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKP_CUPNH

Accession

Primary (citable) accession number: P50320

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program