P50319 (PGKC_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase, chromosomal
EC=2.7.2.3

Gene names

Name:	cbbKC
Synonyms:	cbbK2
Ordered Locus Names:	H16_B1385

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus necator

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145
Pathway	Carbohydrate biosynthesis; Calvin cycle. HAMAP-Rule MF_00145
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm HAMAP-Rule MF_00145.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Phosphoglycerate kinase, chromosomal HAMAP-Rule MF_00145		PRO_0000145895
Regions
	Nucleotide binding	366 – 369	4	ATP By similarity
	Region	40 – 42	3	Substrate binding By similarity
	Region	79 – 82	4	Substrate binding By similarity
Sites
	Binding site	56	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	218	1	ATP By similarity
	Binding site	340	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50319 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6B4C9D195566A90D	FASTA	413	42,284
	10 20 30 40 50 60 MMSLSHATNP QPKPAAAPHT LAALLAAGGL AGKRVFIRAD LNVPQDAAGA ITDDTRIRAS 70 80 90 100 110 120 VPAIAACLQA GAAVMVTSHL GRPQEGAPDP RHSLAPVGRR LSELLGRQVP LLSGWTEGGF 130 140 150 160 170 180 QVPPGQVVLL ENCRMNTGEK KNSDELAQKM AALCDVYVND AFGTAHRAEA TTHGIARYAP 190 200 210 220 230 240 IACAGPLLAA EIDALGKALG QPARPLVAIV AGSKVSTKLT ILKSLADKVD NLIVGGGIAN 250 260 270 280 290 300 TFMLAAGLKI GKSLAEPDLL ADARAIIDIM ARRGASVPIP VDVVCAKEFS ATAAATVKDV 310 320 330 340 350 360 KDVADDDMIL DIGPKTAAML AEQLKAAGTI VWNGPVGVFE FDQFGNGTRV LAQAIAESKA 370 380 390 400 410 FSIAGGGDTL AAIAKHGIAD RVGYISTGGG AFLEFLEGKT LPALAVLAQR AAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the genes forming the distal parts of the two cbb CO2 fixation operons from Alcaligenes eutrophus." Schaeferfohann J., Yoo J.-G., Bowien B. Arch. Microbiol. 163:291-299(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12422 Genomic DNA. Translation: AAC43444.1. AM260480 Genomic DNA. Translation: CAJ96174.1.
PIR	I39551.
RefSeq	YP_840904.1. NC_008314.1.
3D structure databases
ProteinModelPortal	P50319.
SMR	P50319. Positions 30-411.
ModBase	Search...
Protein-protein interaction databases
STRING	381666.H16_B1385.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ96174; CAJ96174; H16_B1385.
GeneID	4455911.
KEGG	reh:H16_B1385.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0126.
HOGENOM	HOG000227107.
KO	K00927.
OMA	MANDSIG.
ProtClustDB	PRK00073.
Enzyme and pathway databases
BioCyc	CNEC381666:GJUJ-5090-MONOMER.
UniPathway	UPA00116.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
HAMAP	MF_00145. Phosphoglyc_kinase.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKC_CUPNH

Accession

Primary (citable) accession number: P50319
Secondary accession number(s): Q0K1F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program