Reviewed, UniProtKB/Swiss-Prot P50319 (PGKC_RALEH)

Names and origin

Protein names

Recommended name:
    Phosphoglycerate kinase, chromosomal
    EC=2.7.2.3

Gene names

Name:	cbbKC
Synonyms:	cbbK2
Ordered Locus Names:	H16_B1385

Organism

Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP MF_00145
Pathway	Carbohydrate biosynthesis; Calvin cycle. HAMAP MF_00145
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00145
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Phosphoglycerate kinase, chromosomal HAMAP MF_00145		PRO_0000145895
Regions
	Nucleotide binding	366 – 369	4	ATP By similarity
	Region	40 – 42	3	Substrate binding By similarity
	Region	79 – 82	4	Substrate binding By similarity
Sites
	Binding site	56	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	218	1	ATP By similarity
	Binding site	340	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50319-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6B4C9D195566A90D	FASTA	413	42,284
	10 20 30 40 50 60 MMSLSHATNP QPKPAAAPHT LAALLAAGGL AGKRVFIRAD LNVPQDAAGA ITDDTRIRAS 70 80 90 100 110 120 VPAIAACLQA GAAVMVTSHL GRPQEGAPDP RHSLAPVGRR LSELLGRQVP LLSGWTEGGF 130 140 150 160 170 180 QVPPGQVVLL ENCRMNTGEK KNSDELAQKM AALCDVYVND AFGTAHRAEA TTHGIARYAP 190 200 210 220 230 240 IACAGPLLAA EIDALGKALG QPARPLVAIV AGSKVSTKLT ILKSLADKVD NLIVGGGIAN 250 260 270 280 290 300 TFMLAAGLKI GKSLAEPDLL ADARAIIDIM ARRGASVPIP VDVVCAKEFS ATAAATVKDV 310 320 330 340 350 360 KDVADDDMIL DIGPKTAAML AEQLKAAGTI VWNGPVGVFE FDQFGNGTRV LAQAIAESKA 370 380 390 400 410 FSIAGGGDTL AAIAKHGIAD RVGYISTGGG AFLEFLEGKT LPALAVLAQR AAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the genes forming the distal parts of the two cbb CO2 fixation operons from Alcaligenes eutrophus." Schaeferfohann J., Yoo J.-G., Bowien B. Arch. Microbiol. 163:291-299(1995) [PubMed: 7763137] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U12422 Genomic DNA. Translation: AAC43444.1. AM260480 Genomic DNA. Translation: CAJ96174.1.
PIR	I39551.
RefSeq	YP_840904.1.
3D structure databases
HSSP	HSSP built from PDB template 1PHP based on UniProtKB P18912.
ModBase	Search...
Protein-protein interaction databases
STRING	P50319.
Genome annotation databases
GeneID	4455911.
GenomeReviews	Gene locus H16_B1385 in contig AM260480_GR.
KEGG	reh:H16_B1385.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P50319.
OMA	FAADCVG.
Family and domain databases
HAMAP	MF_00145. [Tree]
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKC_RALEH

Accession

Primary (citable) accession number: P50319
Secondary accession number(s): Q0K1F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)