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Protein

Phosphoglycerate kinase 2, chloroplastic

Gene

At1g56190

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei113 – 1131SubstrateBy similarity
Binding sitei194 – 1941SubstrateBy similarity
Binding sitei227 – 2271SubstrateBy similarity
Binding sitei278 – 2781ATPBy similarity
Binding sitei369 – 3691ATP; via carbonyl oxygenBy similarity
Binding sitei400 – 4001ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi429 – 4324ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoglycerate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: InterPro
  2. reductive pentose-phosphate cycle Source: UniProtKB-UniPathway
  3. response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Calvin cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G56190-MONOMER.
ARA:GQT-675-MONOMER.
ReactomeiREACT_239253. Gluconeogenesis.
REACT_248260. Glycolysis.
UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 2, chloroplastic (EC:2.7.2.3)
Gene namesi
Ordered Locus Names:At1g56190
ORF Names:F14G9.19, T6H22.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G56190.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
  3. membrane Source: TAIR
  4. mitochondrion Source: TAIR
  5. thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7373ChloroplastSequence AnalysisAdd
BLAST
Chaini74 – 478405Phosphoglycerate kinase 2, chloroplasticPRO_0000023889Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei78 – 781PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP50318.
PRIDEiP50318.

Expressioni

Gene expression databases

ExpressionAtlasiP50318. baseline and differential.
GenevestigatoriP50318.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi27297. 1 interaction.
STRINGi3702.AT1G56190.1-P.

Structurei

3D structure databases

ProteinModelPortaliP50318.
SMRiP50318. Positions 35-472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni97 – 993Substrate bindingBy similarity
Regioni136 – 1394Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0126.
HOGENOMiHOG000227107.
InParanoidiP50318.
KOiK00927.
OMAiITMAKKS.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P50318-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTAATAAL SIIKSTGGAA VTRSSRASFG HIPSTSVSAR RLGFSAVVDS
60 70 80 90 100
RFSVHVASKV HSVRGKGARG VITMAKKSVG DLNSVDLKGK KVFVRADLNV
110 120 130 140 150
PLDDNQNITD DTRIRAAIPT IKFLIENGAK VILSTHLGRP KGVTPKFSLA
160 170 180 190 200
PLVPRLSELL GIEVVKADDC IGPEVETLVA SLPEGGVLLL ENVRFYKEEE
210 220 230 240 250
KNEPDFAKKL ASLADLYVND AFGTAHRAHA STEGVTKFLK PSVAGFLLQK
260 270 280 290 300
ELDYLVGAVS NPKRPFAAIV GGSKVSSKIG VIESLLEKCD ILLLGGGMIF
310 320 330 340 350
TFYKAQGLSV GSSLVEEDKL ELATTLLAKA KARGVSLLLP TDVVIADKFA
360 370 380 390 400
PDANSKIVPA SAIPDGWMGL DIGPDSVKTF NEALDTTQTV IWNGPMGVFE
410 420 430 440 450
FEKFAKGTEA VANKLAELSK KGVTTIIGGG DSVAAVEKVG VAGVMSHIST
460 470
GGGASLELLE GKVLPGVVAL DEATPVTV
Length:478
Mass (Da):49,939
Last modified:May 3, 2011 - v3
Checksum:i03751560F052E6F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti412 – 4121A → R in AAA79705 (PubMed:10520454).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009894 Genomic DNA. Translation: AAF02830.1.
AC069159 Genomic DNA. Translation: AAG50920.1.
CP002684 Genomic DNA. Translation: AEE33356.1.
AY056291 mRNA. Translation: AAL07140.1.
AY099598 mRNA. Translation: AAM20449.1.
BT000250 mRNA. Translation: AAN15569.1.
U37700 mRNA. Translation: AAA79705.1.
PIRiD96603.
S71214.
RefSeqiNP_176015.1. NM_104498.3. [P50318-1]
UniGeneiAt.11481.

Genome annotation databases

EnsemblPlantsiAT1G56190.1; AT1G56190.1; AT1G56190. [P50318-1]
GeneIDi842072.
KEGGiath:AT1G56190.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009894 Genomic DNA. Translation: AAF02830.1.
AC069159 Genomic DNA. Translation: AAG50920.1.
CP002684 Genomic DNA. Translation: AEE33356.1.
AY056291 mRNA. Translation: AAL07140.1.
AY099598 mRNA. Translation: AAM20449.1.
BT000250 mRNA. Translation: AAN15569.1.
U37700 mRNA. Translation: AAA79705.1.
PIRiD96603.
S71214.
RefSeqiNP_176015.1. NM_104498.3. [P50318-1]
UniGeneiAt.11481.

3D structure databases

ProteinModelPortaliP50318.
SMRiP50318. Positions 35-472.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27297. 1 interaction.
STRINGi3702.AT1G56190.1-P.

Proteomic databases

PaxDbiP50318.
PRIDEiP50318.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G56190.1; AT1G56190.1; AT1G56190. [P50318-1]
GeneIDi842072.
KEGGiath:AT1G56190.

Organism-specific databases

TAIRiAT1G56190.

Phylogenomic databases

eggNOGiCOG0126.
HOGENOMiHOG000227107.
InParanoidiP50318.
KOiK00927.
OMAiITMAKKS.

Enzyme and pathway databases

UniPathwayiUPA00116.
BioCyciARA:AT1G56190-MONOMER.
ARA:GQT-675-MONOMER.
ReactomeiREACT_239253. Gluconeogenesis.
REACT_248260. Glycolysis.

Gene expression databases

ExpressionAtlasiP50318. baseline and differential.
GenevestigatoriP50318.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Two phosphoglycerate kinase cDNAs from Arabidopsis thaliana."
    Loebler M.
    DNA Seq. 8:247-252(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 248-478.
    Strain: cv. Columbia.
    Tissue: Leaf.

Entry informationi

Entry nameiPGKH2_ARATH
AccessioniPrimary (citable) accession number: P50318
Secondary accession number(s): Q42542, Q9C7J4, Q9SGU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 3, 2011
Last modified: March 4, 2015
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.