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Protein

Phosphoglycerate kinase 2, chloroplastic

Gene

At1g56190

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei113SubstrateBy similarity1
Binding sitei194SubstrateBy similarity1
Binding sitei227SubstrateBy similarity1
Binding sitei278ATPBy similarity1
Binding sitei369ATP; via carbonyl oxygenBy similarity1
Binding sitei400ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi429 – 432ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • glycolytic process Source: InterPro
  • reductive pentose-phosphate cycle Source: UniProtKB-UniPathway
  • response to cadmium ion Source: TAIR

Keywordsi

Molecular functionKinase, Transferase
Biological processCalvin cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G56190-MONOMER.
ReactomeiR-ATH-70171. Glycolysis.
R-ATH-70263. Gluconeogenesis.
UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 2, chloroplastic (EC:2.7.2.3)
Gene namesi
Ordered Locus Names:At1g56190
ORF Names:F14G9.19, T6H22.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G56190.
TAIRilocus:2205215. AT1G56190.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 74ChloroplastCuratedAdd BLAST74
ChainiPRO_000002388975 – 478Phosphoglycerate kinase 2, chloroplasticAdd BLAST404

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP50318.
PRIDEiP50318.

PTM databases

iPTMnetiP50318.

Expressioni

Gene expression databases

ExpressionAtlasiP50318. baseline and differential.
GenevisibleiP50318. AT.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi27297. 1 interactor.
STRINGi3702.AT1G56190.1.

Structurei

3D structure databases

ProteinModelPortaliP50318.
SMRiP50318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 99Substrate bindingBy similarity3
Regioni136 – 139Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1367. Eukaryota.
COG0126. LUCA.
HOGENOMiHOG000227107.
InParanoidiP50318.
KOiK00927.
OMAiLICLTAR.
OrthoDBiEOG09360DE5.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 2 hits.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiView protein in InterPro
IPR001576. Phosphoglycerate_kinase.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
IPR036043. Phosphoglycerate_kinase_sf.
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiView protein in Pfam
PF00162. PGK. 1 hit.
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiView protein in PROSITE
PS00111. PGLYCERATE_KINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P50318-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTAATAAL SIIKSTGGAA VTRSSRASFG HIPSTSVSAR RLGFSAVVDS
60 70 80 90 100
RFSVHVASKV HSVRGKGARG VITMAKKSVG DLNSVDLKGK KVFVRADLNV
110 120 130 140 150
PLDDNQNITD DTRIRAAIPT IKFLIENGAK VILSTHLGRP KGVTPKFSLA
160 170 180 190 200
PLVPRLSELL GIEVVKADDC IGPEVETLVA SLPEGGVLLL ENVRFYKEEE
210 220 230 240 250
KNEPDFAKKL ASLADLYVND AFGTAHRAHA STEGVTKFLK PSVAGFLLQK
260 270 280 290 300
ELDYLVGAVS NPKRPFAAIV GGSKVSSKIG VIESLLEKCD ILLLGGGMIF
310 320 330 340 350
TFYKAQGLSV GSSLVEEDKL ELATTLLAKA KARGVSLLLP TDVVIADKFA
360 370 380 390 400
PDANSKIVPA SAIPDGWMGL DIGPDSVKTF NEALDTTQTV IWNGPMGVFE
410 420 430 440 450
FEKFAKGTEA VANKLAELSK KGVTTIIGGG DSVAAVEKVG VAGVMSHIST
460 470
GGGASLELLE GKVLPGVVAL DEATPVTV
Length:478
Mass (Da):49,939
Last modified:May 3, 2011 - v3
Checksum:i03751560F052E6F8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti412A → R in AAA79705 (PubMed:10520454).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009894 Genomic DNA. Translation: AAF02830.1.
AC069159 Genomic DNA. Translation: AAG50920.1.
CP002684 Genomic DNA. Translation: AEE33356.1.
AY056291 mRNA. Translation: AAL07140.1.
AY099598 mRNA. Translation: AAM20449.1.
BT000250 mRNA. Translation: AAN15569.1.
U37700 mRNA. Translation: AAA79705.1.
PIRiD96603.
S71214.
RefSeqiNP_176015.1. NM_104498.4. [P50318-1]
UniGeneiAt.11481.

Genome annotation databases

EnsemblPlantsiAT1G56190.1; AT1G56190.1; AT1G56190. [P50318-1]
GeneIDi842072.
GrameneiAT1G56190.1; AT1G56190.1; AT1G56190.
KEGGiath:AT1G56190.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPGKH2_ARATH
AccessioniPrimary (citable) accession number: P50318
Secondary accession number(s): Q42542, Q9C7J4, Q9SGU6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 3, 2011
Last modified: November 22, 2017
This is version 123 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families