Reviewed, UniProtKB/Swiss-Prot P50318 (PGKH_ARATH)

Names and origin

Protein names

Recommended name:
    Phosphoglycerate kinase, chloroplastic
    EC=2.7.2.3

Gene names

Ordered Locus Names:	At1g56190
ORF Names:	T6H22.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Monomer By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR membrane Inferred from direct assay. Source: TAIR mitochondrion Inferred from direct assay. Source: TAIR thylakoid Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P50318-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 73	73	Chloroplast Potential
	Chain	74 – 478	405	Phosphoglycerate kinase, chloroplastic		PRO_0000023889
Regions
	Nucleotide binding	429 – 432	4	ATP By similarity
	Region	97 – 99	3	Substrate binding By similarity
	Region	136 – 139	4	Substrate binding By similarity
Sites
	Binding site	113	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity
	Binding site	227	1	Substrate By similarity
	Binding site	278	1	ATP By similarity
	Binding site	369	1	ATP; via carbonyl oxygen By similarity
	Binding site	400	1	ATP By similarity
Experimental info
	Sequence conflict	83 – 85	3	NSV → TSA in AAB60303. Ref.2
	Sequence conflict	107	1	N → T in AAB60303. Ref.2
	Sequence conflict	123	1	F → Y in AAB60303. Ref.2
	Sequence conflict	165	1	V → T in AAB60303. Ref.2
	Sequence conflict	177	1	T → S in AAB60303. Ref.2
	Sequence conflict	203 – 205	3	EPD → DPE in AAB60303. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: A1FE198F5DEDC1B8	FASTA	478	49,925
	10 20 30 40 50 60 MASTAATAAL SIIKSTGGAA VTRSSRASFG HIPSTSVSAR RLGFSAVVDS RFSVHVASKV 70 80 90 100 110 120 HSVRGKGARG VITMAKKSVG DLNSVDLKGK KVFVRADLNV PLDDNQNITD DTRIRAAIPT 130 140 150 160 170 180 IKFLIENGAK VILSTHLGRP KGVTPKFSLA PLVPRLSELL GIEVVKADDC IGPEVETLVA 190 200 210 220 230 240 SLPEGGVLLL ENVRFYKEEE KNEPDFAKKL ASLADLYVND AFGTAHRAHA STEGVTKFLK 250 260 270 280 290 300 PSVAGFLLQK ELDYLVGAVS NPKRPFAAIV GGSKVSSKIG VIESLLEKCD ILLLGGGMIF 310 320 330 340 350 360 TFYKAQGLSV GSSLVEEDKL ELATELLAKA KAKGVSLLLP TDVVVADKFA PDANSKIVPA 370 380 390 400 410 420 SGIEDGWMGL DIGPDSIKTF NEALDTTQTV IWNGPMGVFE MEKFAAGTEA IANKLAELSE 430 440 450 460 470 KGVTTIIGGG DSVAAVEKVG VAGVMSHIST GGGASLELLE GKVLPGVIAL DEAIPVTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	Loebler M. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 80-478. Strain: cv. Columbia. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC009894 Genomic DNA. Translation: AAF02830.1. U37701 mRNA. Translation: AAB60303.1.
IPI	IPI00530695.
PIR	S71368.
3D structure databases
HSSP	HSSP built from PDB template 1PHP based on UniProtKB P18912.
ModBase	Search...
Protein-protein interaction databases
STRING	P50318.
Proteomic databases
PRIDE	P50318.
ProMEX	P50318.
Genome annotation databases
GenomeReviews	Gene locus AT1G56190 in contig CT485782_GR.
Organism-specific databases
TAIR	At1g56190.
Enzyme and pathway databases
BRENDA	2.7.2.3. 302.
Gene expression databases
Genevestigator	P50318.
Family and domain databases
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKH_ARATH

Accession

Primary (citable) accession number: P50318
Secondary accession number(s): Q9SGU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 1, 2000
Last modified:	October 13, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)