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P50318

- PGKH2_ARATH

UniProt

P50318 - PGKH2_ARATH

Protein

Phosphoglycerate kinase 2, chloroplastic

Gene

At1g56190

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 3 (03 May 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei113 – 1131SubstrateBy similarity
    Binding sitei194 – 1941SubstrateBy similarity
    Binding sitei227 – 2271SubstrateBy similarity
    Binding sitei278 – 2781ATPBy similarity
    Binding sitei369 – 3691ATP; via carbonyl oxygenBy similarity
    Binding sitei400 – 4001ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi429 – 4324ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. phosphoglycerate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: InterPro
    2. reductive pentose-phosphate cycle Source: UniProtKB-UniPathway
    3. response to cadmium ion Source: TAIR

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Calvin cycle

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G56190-MONOMER.
    ARA:GQT-675-MONOMER.
    UniPathwayiUPA00116.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoglycerate kinase 2, chloroplastic (EC:2.7.2.3)
    Gene namesi
    Ordered Locus Names:At1g56190
    ORF Names:F14G9.19, T6H22.1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G56190.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR
    3. membrane Source: TAIR
    4. mitochondrion Source: TAIR
    5. thylakoid Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 7373ChloroplastSequence AnalysisAdd
    BLAST
    Chaini74 – 478405Phosphoglycerate kinase 2, chloroplasticPRO_0000023889Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei78 – 781PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP50318.
    PRIDEiP50318.

    Expressioni

    Gene expression databases

    GenevestigatoriP50318.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    BioGridi27297. 1 interaction.
    STRINGi3702.AT1G56190.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP50318.
    SMRiP50318. Positions 35-472.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni97 – 993Substrate bindingBy similarity
    Regioni136 – 1394Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the phosphoglycerate kinase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0126.
    HOGENOMiHOG000227107.
    InParanoidiP50318.
    KOiK00927.
    OMAiMSIRYID.

    Family and domain databases

    Gene3Di3.40.50.1260. 1 hit.
    3.40.50.1270. 1 hit.
    HAMAPiMF_00145. Phosphoglyc_kinase.
    InterProiIPR001576. Phosphoglycerate_kinase.
    IPR015901. Phosphoglycerate_kinase_C.
    IPR015911. Phosphoglycerate_kinase_CS.
    IPR015824. Phosphoglycerate_kinase_N.
    [Graphical view]
    PANTHERiPTHR11406. PTHR11406. 1 hit.
    PfamiPF00162. PGK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000724. Pgk. 1 hit.
    PRINTSiPR00477. PHGLYCKINASE.
    SUPFAMiSSF53748. SSF53748. 1 hit.
    PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: P50318-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MASTAATAAL SIIKSTGGAA VTRSSRASFG HIPSTSVSAR RLGFSAVVDS    50
    RFSVHVASKV HSVRGKGARG VITMAKKSVG DLNSVDLKGK KVFVRADLNV 100
    PLDDNQNITD DTRIRAAIPT IKFLIENGAK VILSTHLGRP KGVTPKFSLA 150
    PLVPRLSELL GIEVVKADDC IGPEVETLVA SLPEGGVLLL ENVRFYKEEE 200
    KNEPDFAKKL ASLADLYVND AFGTAHRAHA STEGVTKFLK PSVAGFLLQK 250
    ELDYLVGAVS NPKRPFAAIV GGSKVSSKIG VIESLLEKCD ILLLGGGMIF 300
    TFYKAQGLSV GSSLVEEDKL ELATTLLAKA KARGVSLLLP TDVVIADKFA 350
    PDANSKIVPA SAIPDGWMGL DIGPDSVKTF NEALDTTQTV IWNGPMGVFE 400
    FEKFAKGTEA VANKLAELSK KGVTTIIGGG DSVAAVEKVG VAGVMSHIST 450
    GGGASLELLE GKVLPGVVAL DEATPVTV 478
    Length:478
    Mass (Da):49,939
    Last modified:May 3, 2011 - v3
    Checksum:i03751560F052E6F8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti412 – 4121A → R in AAA79705. (PubMed:10520454)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC009894 Genomic DNA. Translation: AAF02830.1.
    AC069159 Genomic DNA. Translation: AAG50920.1.
    CP002684 Genomic DNA. Translation: AEE33356.1.
    AY056291 mRNA. Translation: AAL07140.1.
    AY099598 mRNA. Translation: AAM20449.1.
    BT000250 mRNA. Translation: AAN15569.1.
    U37700 mRNA. Translation: AAA79705.1.
    PIRiD96603.
    S71214.
    RefSeqiNP_176015.1. NM_104498.3. [P50318-1]
    UniGeneiAt.11481.

    Genome annotation databases

    EnsemblPlantsiAT1G56190.1; AT1G56190.1; AT1G56190. [P50318-1]
    GeneIDi842072.
    KEGGiath:AT1G56190.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC009894 Genomic DNA. Translation: AAF02830.1 .
    AC069159 Genomic DNA. Translation: AAG50920.1 .
    CP002684 Genomic DNA. Translation: AEE33356.1 .
    AY056291 mRNA. Translation: AAL07140.1 .
    AY099598 mRNA. Translation: AAM20449.1 .
    BT000250 mRNA. Translation: AAN15569.1 .
    U37700 mRNA. Translation: AAA79705.1 .
    PIRi D96603.
    S71214.
    RefSeqi NP_176015.1. NM_104498.3. [P50318-1 ]
    UniGenei At.11481.

    3D structure databases

    ProteinModelPortali P50318.
    SMRi P50318. Positions 35-472.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 27297. 1 interaction.
    STRINGi 3702.AT1G56190.1-P.

    Proteomic databases

    PaxDbi P50318.
    PRIDEi P50318.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G56190.1 ; AT1G56190.1 ; AT1G56190 . [P50318-1 ]
    GeneIDi 842072.
    KEGGi ath:AT1G56190.

    Organism-specific databases

    TAIRi AT1G56190.

    Phylogenomic databases

    eggNOGi COG0126.
    HOGENOMi HOG000227107.
    InParanoidi P50318.
    KOi K00927.
    OMAi MSIRYID.

    Enzyme and pathway databases

    UniPathwayi UPA00116 .
    BioCyci ARA:AT1G56190-MONOMER.
    ARA:GQT-675-MONOMER.

    Gene expression databases

    Genevestigatori P50318.

    Family and domain databases

    Gene3Di 3.40.50.1260. 1 hit.
    3.40.50.1270. 1 hit.
    HAMAPi MF_00145. Phosphoglyc_kinase.
    InterProi IPR001576. Phosphoglycerate_kinase.
    IPR015901. Phosphoglycerate_kinase_C.
    IPR015911. Phosphoglycerate_kinase_CS.
    IPR015824. Phosphoglycerate_kinase_N.
    [Graphical view ]
    PANTHERi PTHR11406. PTHR11406. 1 hit.
    Pfami PF00162. PGK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000724. Pgk. 1 hit.
    PRINTSi PR00477. PHGLYCKINASE.
    SUPFAMi SSF53748. SSF53748. 1 hit.
    PROSITEi PS00111. PGLYCERATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Two phosphoglycerate kinase cDNAs from Arabidopsis thaliana."
      Loebler M.
      DNA Seq. 8:247-252(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 248-478.
      Strain: cv. Columbia.
      Tissue: Leaf.

    Entry informationi

    Entry nameiPGKH2_ARATH
    AccessioniPrimary (citable) accession number: P50318
    Secondary accession number(s): Q42542, Q9C7J4, Q9SGU6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 99 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3