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P50318 (PGKH2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase 2, chloroplastic

EC=2.7.2.3
Gene names
Ordered Locus Names:At1g56190
ORF Names:F14G9.19, T6H22.1
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate biosynthesis; Calvin cycle. HAMAP-Rule MF_00145

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00145

Subcellular location

Plastidchloroplast HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P50318-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 7373Chloroplast Potential
Chain74 – 478405Phosphoglycerate kinase 2, chloroplastic HAMAP-Rule MF_00145
PRO_0000023889

Regions

Nucleotide binding429 – 4324ATP By similarity
Region97 – 993Substrate binding By similarity
Region136 – 1394Substrate binding By similarity

Sites

Binding site1131Substrate By similarity
Binding site1941Substrate By similarity
Binding site2271Substrate By similarity
Binding site2781ATP By similarity
Binding site3691ATP; via carbonyl oxygen By similarity
Binding site4001ATP By similarity

Amino acid modifications

Modified residue781Phosphoserine By similarity

Experimental info

Sequence conflict4121A → R in AAA79705. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 3, 2011. Version 3.
Checksum: 03751560F052E6F8

FASTA47849,939
        10         20         30         40         50         60 
MASTAATAAL SIIKSTGGAA VTRSSRASFG HIPSTSVSAR RLGFSAVVDS RFSVHVASKV 

        70         80         90        100        110        120 
HSVRGKGARG VITMAKKSVG DLNSVDLKGK KVFVRADLNV PLDDNQNITD DTRIRAAIPT 

       130        140        150        160        170        180 
IKFLIENGAK VILSTHLGRP KGVTPKFSLA PLVPRLSELL GIEVVKADDC IGPEVETLVA 

       190        200        210        220        230        240 
SLPEGGVLLL ENVRFYKEEE KNEPDFAKKL ASLADLYVND AFGTAHRAHA STEGVTKFLK 

       250        260        270        280        290        300 
PSVAGFLLQK ELDYLVGAVS NPKRPFAAIV GGSKVSSKIG VIESLLEKCD ILLLGGGMIF 

       310        320        330        340        350        360 
TFYKAQGLSV GSSLVEEDKL ELATTLLAKA KARGVSLLLP TDVVIADKFA PDANSKIVPA 

       370        380        390        400        410        420 
SAIPDGWMGL DIGPDSVKTF NEALDTTQTV IWNGPMGVFE FEKFAKGTEA VANKLAELSK 

       430        440        450        460        470 
KGVTTIIGGG DSVAAVEKVG VAGVMSHIST GGGASLELLE GKVLPGVVAL DEATPVTV 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Two phosphoglycerate kinase cDNAs from Arabidopsis thaliana."
Loebler M.
DNA Seq. 8:247-252(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 248-478.
Strain: cv. Columbia.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC009894 Genomic DNA. Translation: AAF02830.1.
AC069159 Genomic DNA. Translation: AAG50920.1.
CP002684 Genomic DNA. Translation: AEE33356.1.
AY056291 mRNA. Translation: AAL07140.1.
AY099598 mRNA. Translation: AAM20449.1.
BT000250 mRNA. Translation: AAN15569.1.
U37700 mRNA. Translation: AAA79705.1.
PIRD96603.
S71214.
RefSeqNP_176015.1. NM_104498.3.
UniGeneAt.11481.

3D structure databases

ProteinModelPortalP50318.
SMRP50318. Positions 35-472.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid27297. 1 interaction.
STRING3702.AT1G56190.1-P.

Proteomic databases

PaxDbP50318.
PRIDEP50318.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G56190.1; AT1G56190.1; AT1G56190. [P50318-1]
GeneID842072.
KEGGath:AT1G56190.

Organism-specific databases

TAIRAT1G56190.

Phylogenomic databases

eggNOGCOG0126.
HOGENOMHOG000227107.
InParanoidP50318.
KOK00927.
OMANKMFGYL.
ProtClustDBPLN03034.

Enzyme and pathway databases

BioCycARA:AT1G56190-MONOMER.
ARA:GQT-675-MONOMER.
UniPathwayUPA00116.

Gene expression databases

GenevestigatorP50318.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGKH2_ARATH
AccessionPrimary (citable) accession number: P50318
Secondary accession number(s): Q42542, Q9C7J4, Q9SGU6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 3, 2011
Last modified: April 16, 2014
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names