P50313 (PGK_TETTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:

PGK

Organism

Tetrahymena thermophila

Taxonomic identifier

5911 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Alveolata › Ciliophora › Intramacronucleata › Oligohymenophorea › Hymenostomatida › Tetrahymenina › Tetrahymenidae › Tetrahymena

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Phosphoglycerate kinase		PRO_0000145861
Regions
	Nucleotide binding	373 – 376	4	ATP By similarity
	Region	25 – 27	3	Substrate binding By similarity
	Region	64 – 67	4	Substrate binding By similarity
Sites
	Binding site	40	1	Substrate By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	171	1	Substrate By similarity
	Binding site	220	1	ATP By similarity
	Binding site	313	1	ATP; via carbonyl oxygen By similarity
	Binding site	344	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50313 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: FCCE8F842FEA8016	FASTA	420	45,914
	10 20 30 40 50 60 MLSRKIGIDH ILKHIENKRV LMRVDFNVPL KEGKVKDPTR IQGSVPSIKK ILEQNPKGLV 70 80 90 100 110 120 LMSHLGRPDG NRVEKHSMKP VVPKLEELLG TKVTFLNDCV GKDVEEAVKS SRNGEIILLE 130 140 150 160 170 180 NLRFHPEEEG KYIDAAGNKV KADSKAVKEF RKSLTSLGDL FVNDAFGTAH RAHSSMVGVD 190 200 210 220 230 240 HKIRAAGYLL KRELDYFSKA LESPNRPFLV VLGGAKVKDK IQLIESMLDK VDEMIIGGGM 250 260 270 280 290 300 AFTFLKKIHN IEIGNSLFDE EGYKIVDQLL EKAKAKNVKI HLPVDFLCGD SLEANANTRS 310 320 330 340 350 360 FDLKSGIPAG WIGLDAGPKT MAENAEAVAR ANTIVWNGPQ GRFEVDKFKV GSSDLLKHVA 370 380 390 400 410 420 TRTSQGATSI IGGGDTVNLV QQEKATQKVS HVSTGGGASL ELLEGKVLPG VAYLTDIDQL

References

[1]	"A phylogenetic analysis based on the gene encoding phosphoglycerate kinase." Vohra G.B., Golding G.B., Tsao N., Pearlman R.E. J. Mol. Evol. 34:383-395(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: CU329.
[2]	Erratum Vohra G.B., Golding G.B., Tsao N., Pearlman R.E. J. Mol. Evol. 35:466-466(1992) [PubMed] [Europe PMC] [Abstract]

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63529 mRNA. Translation: CAA45092.1. X63528 Genomic DNA. Translation: CAA45091.1.
RefSeq	XP_001025708.1. XM_001025708.2.
UniGene	Tth.90.
3D structure databases
ProteinModelPortal	P50313.
SMR	P50313. Positions 5-418.
ModBase	Search...
Protein-protein interaction databases
STRING	5911.P50313.
Proteomic databases
PRIDE	P50313.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7824345.
KEGG	tet:TTHERM_00929450.
Phylogenomic databases
eggNOG	COG0126.
KO	K00927.
ProtClustDB	PTZ00005.
Enzyme and pathway databases
UniPathway	UPA00109; UER00185.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_TETTH

Accession

Primary (citable) accession number: P50313

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)