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Protein

Phosphoglycerate kinase

Gene

PGK

Organism
Tetrahymena thermophila
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathway: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GapC)
  2. Phosphoglycerate kinase (PGK)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401SubstrateBy similarity
Binding sitei123 – 1231SubstrateBy similarity
Binding sitei171 – 1711SubstrateBy similarity
Binding sitei220 – 2201ATPBy similarity
Binding sitei313 – 3131ATP; via carbonyl oxygenBy similarity
Binding sitei344 – 3441ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi373 – 3764ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:PGK
OrganismiTetrahymena thermophila
Taxonomic identifieri5911 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420Phosphoglycerate kinasePRO_0000145861Add
BLAST

Proteomic databases

PRIDEiP50313.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi5911.EAS05463.

Structurei

3D structure databases

ProteinModelPortaliP50313.
SMRiP50313. Positions 5-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 273Substrate bindingBy similarity
Regioni64 – 674Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0126.
KOiK00927.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50313-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSRKIGIDH ILKHIENKRV LMRVDFNVPL KEGKVKDPTR IQGSVPSIKK
60 70 80 90 100
ILEQNPKGLV LMSHLGRPDG NRVEKHSMKP VVPKLEELLG TKVTFLNDCV
110 120 130 140 150
GKDVEEAVKS SRNGEIILLE NLRFHPEEEG KYIDAAGNKV KADSKAVKEF
160 170 180 190 200
RKSLTSLGDL FVNDAFGTAH RAHSSMVGVD HKIRAAGYLL KRELDYFSKA
210 220 230 240 250
LESPNRPFLV VLGGAKVKDK IQLIESMLDK VDEMIIGGGM AFTFLKKIHN
260 270 280 290 300
IEIGNSLFDE EGYKIVDQLL EKAKAKNVKI HLPVDFLCGD SLEANANTRS
310 320 330 340 350
FDLKSGIPAG WIGLDAGPKT MAENAEAVAR ANTIVWNGPQ GRFEVDKFKV
360 370 380 390 400
GSSDLLKHVA TRTSQGATSI IGGGDTVNLV QQEKATQKVS HVSTGGGASL
410 420
ELLEGKVLPG VAYLTDIDQL
Length:420
Mass (Da):45,914
Last modified:October 1, 1996 - v1
Checksum:iFCCE8F842FEA8016
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63529 mRNA. Translation: CAA45092.1.
X63528 Genomic DNA. Translation: CAA45091.1.
RefSeqiXP_001025708.1. XM_001025708.2.
UniGeneiTth.90.

Genome annotation databases

GeneIDi7824345.
KEGGitet:TTHERM_00929450.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63529 mRNA. Translation: CAA45092.1.
X63528 Genomic DNA. Translation: CAA45091.1.
RefSeqiXP_001025708.1. XM_001025708.2.
UniGeneiTth.90.

3D structure databases

ProteinModelPortaliP50313.
SMRiP50313. Positions 5-418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5911.EAS05463.

Proteomic databases

PRIDEiP50313.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi7824345.
KEGGitet:TTHERM_00929450.

Phylogenomic databases

eggNOGiCOG0126.
KOiK00927.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A phylogenetic analysis based on the gene encoding phosphoglycerate kinase."
    Vohra G.B., Golding G.B., Tsao N., Pearlman R.E.
    J. Mol. Evol. 34:383-395(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: CU329.
  2. Erratum
    Vohra G.B., Golding G.B., Tsao N., Pearlman R.E.
    J. Mol. Evol. 35:466-466(1992) [PubMed] [Europe PMC] [Abstract]

Entry informationi

Entry nameiPGK_TETTH
AccessioniPrimary (citable) accession number: P50313
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 24, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.