P50308 (IPYR_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	Saci_0955

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	173 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and that are present before substrate binds, two additional magnesium ions form complexes with substrate and product.
Subunit structure	Homohexamer.
Subcellular location	Cytoplasm HAMAP MF_00209.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 173

173

Inorganic pyrophosphatase HAMAP MF_00209

PRO_0000137559

Sites

Metal binding

62

1

Magnesium 1

Metal binding

67

1

Magnesium 2

Metal binding

99

1

Magnesium 1

Secondary structure

1

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173

Helix Strand Turn

Details...

Turn

7 – 11

5

Beta strand

12 – 19

8

Beta strand

25 – 30

6

Turn

31 – 34

4

Beta strand

35 – 41

7

Beta strand

43 – 46

4

Beta strand

49 – 54

6

Beta strand

67 – 70

4

Beta strand

81 – 94

14

Beta strand

97 – 106

10

Turn

108 – 110

3

Helix

112 – 114

3

Helix

120 – 122

3

Helix

125 – 137

13

Turn

138 – 141

4

Beta strand

147 – 153

7

Helix

155 – 169

15

Sequences

	Sequence		Length	Mass (Da)
	P50308 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 15FF27EED2B79D72	FASTA	173	19,381
	10 20 30 40 50 60 MKLSPGKNAP DVVNVLVEIP QGSNIKYEYD DEEGVIKVDR VLYTSMNYPF NYGFIPGTLE 70 80 90 100 110 120 EDGDPLDVLV ITNYQLYPGS VIEVRPIGIL YMKDEEGEDA KIVAVPKDKT DPSFSNIKDI 130 140 150 160 170 NDLPQATKNK IVHFFEHYKE LEPGKYVKIS GWGSATEAKN RIQLAIKRVS GGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, cloning, and sequencing of archaebacterial pyrophosphatase from the extreme thermoacidophile Sulfolobus acidocaldarius." Meyer W., Moll R., Kath T., Schaefer G. Arch. Biochem. Biophys. 319:149-156(1995) [PubMed: 7771779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
[2]	"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
[3]	"Sulfolobus acidocaldarius inorganic pyrophosphatase: structure, thermostability, and effect of metal ion in an archael pyrophosphatase." Leppaenen V.-M., Nummelin H., Hansen T., Lahti R., Schaefer G., Goldman A. Protein Sci. 8:1218-1231(1999) [PubMed: 10386872] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS). Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X81842 Genomic DNA. Translation: CAA57434.1.
CP000077 Genomic DNA. Translation: AAY80317.1.

PIR

S57617. S65965.

RefSeq

YP_255610.1. NC_007181.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QEZ	X-ray	2.70	A/B/C/D/E/F	1-173	[»]

ProteinModelPortal

P50308.

SMR

P50308. Positions 2-170.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3472897.

GenomeReviews

Gene locus Saci_0955 in contig CP000077_GR.

KEGG

sai:Saci_0955.

NMPDR

fig|330779.3.peg.1112.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG529150.

OMA

PNDFNVI.

PhylomeDB

P50308.

ProtClustDB

PRK01250.

Enzyme and pathway databases

BioCyc

SACI330779:SACI_0955-MONOMER.

Family and domain databases

HAMAP

MF_00209. Inorganic_PPase.
[Tree]

InterPro

IPR008162. Pyrophosphatase.
[Graphical view]

Gene3D

G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.

KO

K01507.

PANTHER

PTHR10286. Pyrophosphatase. 1 hit.

Pfam

PF00719. Pyrophosphatase. 1 hit.
[Graphical view]

SUPFAM

SSF50324. Pyrophosphatase. 1 hit.

PROSITE

PS00387. PPASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

IPYR_SULAC

Accession

Primary (citable) accession number: P50308
Secondary accession number(s): Q4JA63

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program