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Protein

Inorganic pyrophosphatase

Gene

ppa

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Diphosphate + H2O = 2 phosphate.

Cofactori

Mg2+Note: Binds 4 Mg2+ ions per subunit. Other metal ions can support activity, but at a lower rate. Two Mg2+ ions are required for the activation of the enzyme and are present before substrate binds, two additional Mg2+ ions form complexes with substrate and product.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Magnesium 1
Metal bindingi67 – 671Magnesium 2
Metal bindingi99 – 991Magnesium 1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSACI330779:GH9J-940-MONOMER.
BRENDAi3.6.1.1. 6160.

Names & Taxonomyi

Protein namesi
Recommended name:
Inorganic pyrophosphatase (EC:3.6.1.1)
Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name:
PPase
Gene namesi
Name:ppa
Ordered Locus Names:Saci_0955
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001018 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 173173Inorganic pyrophosphatasePRO_0000137559Add
BLAST

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

STRINGi330779.Saci_0955.

Structurei

Secondary structure

1
173
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni7 – 115Combined sources
Beta strandi12 – 198Combined sources
Beta strandi25 – 306Combined sources
Turni31 – 344Combined sources
Beta strandi35 – 417Combined sources
Beta strandi43 – 464Combined sources
Beta strandi49 – 546Combined sources
Beta strandi67 – 704Combined sources
Beta strandi81 – 9414Combined sources
Beta strandi97 – 10610Combined sources
Turni108 – 1103Combined sources
Helixi112 – 1143Combined sources
Helixi120 – 1223Combined sources
Helixi125 – 13713Combined sources
Turni138 – 1414Combined sources
Beta strandi147 – 1537Combined sources
Helixi155 – 16915Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QEZX-ray2.70A/B/C/D/E/F1-173[»]
ProteinModelPortaliP50308.
SMRiP50308. Positions 2-170.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50308.

Family & Domainsi

Sequence similaritiesi

Belongs to the PPase family.Curated

Phylogenomic databases

eggNOGiarCOG01711. Archaea.
COG0221. LUCA.
HOGENOMiHOG000236472.
KOiK01507.
OMAiDGVANYK.

Family and domain databases

Gene3Di3.90.80.10. 1 hit.
HAMAPiMF_00209. Inorganic_PPase.
InterProiIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERiPTHR10286. PTHR10286. 1 hit.
PfamiPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF50324. SSF50324. 1 hit.
PROSITEiPS00387. PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50308-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSPGKNAP DVVNVLVEIP QGSNIKYEYD DEEGVIKVDR VLYTSMNYPF
60 70 80 90 100
NYGFIPGTLE EDGDPLDVLV ITNYQLYPGS VIEVRPIGIL YMKDEEGEDA
110 120 130 140 150
KIVAVPKDKT DPSFSNIKDI NDLPQATKNK IVHFFEHYKE LEPGKYVKIS
160 170
GWGSATEAKN RIQLAIKRVS GGQ
Length:173
Mass (Da):19,381
Last modified:October 1, 1996 - v1
Checksum:i15FF27EED2B79D72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81842 Genomic DNA. Translation: CAA57434.1.
CP000077 Genomic DNA. Translation: AAY80317.1.
PIRiS65965. S57617.
RefSeqiWP_011277819.1. NC_007181.1.

Genome annotation databases

EnsemblBacteriaiAAY80317; AAY80317; Saci_0955.
GeneIDi3472897.
KEGGisai:Saci_0955.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81842 Genomic DNA. Translation: CAA57434.1.
CP000077 Genomic DNA. Translation: AAY80317.1.
PIRiS65965. S57617.
RefSeqiWP_011277819.1. NC_007181.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QEZX-ray2.70A/B/C/D/E/F1-173[»]
ProteinModelPortaliP50308.
SMRiP50308. Positions 2-170.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi330779.Saci_0955.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY80317; AAY80317; Saci_0955.
GeneIDi3472897.
KEGGisai:Saci_0955.

Phylogenomic databases

eggNOGiarCOG01711. Archaea.
COG0221. LUCA.
HOGENOMiHOG000236472.
KOiK01507.
OMAiDGVANYK.

Enzyme and pathway databases

BioCyciSACI330779:GH9J-940-MONOMER.
BRENDAi3.6.1.1. 6160.

Miscellaneous databases

EvolutionaryTraceiP50308.

Family and domain databases

Gene3Di3.90.80.10. 1 hit.
HAMAPiMF_00209. Inorganic_PPase.
InterProiIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERiPTHR10286. PTHR10286. 1 hit.
PfamiPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF50324. SSF50324. 1 hit.
PROSITEiPS00387. PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Purification, cloning, and sequencing of archaebacterial pyrophosphatase from the extreme thermoacidophile Sulfolobus acidocaldarius."
    Meyer W., Moll R., Kath T., Schaefer G.
    Arch. Biochem. Biophys. 319:149-156(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
    Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
  2. "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
    Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
    J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
  3. "Sulfolobus acidocaldarius inorganic pyrophosphatase: structure, thermostability, and effect of metal ion in an archael pyrophosphatase."
    Leppaenen V.-M., Nummelin H., Hansen T., Lahti R., Schaefer G., Goldman A.
    Protein Sci. 8:1218-1231(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
    Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Entry informationi

Entry nameiIPYR_SULAC
AccessioniPrimary (citable) accession number: P50308
Secondary accession number(s): Q4JA63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 11, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.