Reviewed, UniProtKB/Swiss-Prot P50306 (METK4_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable S-adenosylmethionine synthetase 4
      Short name=AdoMet synthetase 4
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 4
      Short name=MAT 4

Gene names

Name:	sams-4
ORF Names:	C06E7.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Sequence similarities	Belongs to the AdoMet synthetase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase positive regulation of locomotion Inferred from mutant phenotype. Source: WormBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Probable S-adenosylmethionine synthetase 4		PRO_0000174443
Regions
	Nucleotide binding	119 – 124	6	ATP Potential
Sites
	Metal binding	19	1	Magnesium By similarity
	Metal binding	45	1	Potassium By similarity
	Metal binding	271	1	Potassium By similarity
	Metal binding	279	1	Magnesium By similarity
	Binding site	147	1	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P50306-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D7D9EADDC59789B3	FASTA	404	43,960
	10 20 30 40 50 60 MPQHKFLFTS ESVSEGHPDK MCDQISDAVL DAHLAQDPHA KVACETVTKT GMIMLCGEIT 70 80 90 100 110 120 SKAVVDYQVL VRNVIKKIGY DDSSKGFDYK TCNVLVALEQ QSPEIAAGVH VDKDSDDVGA 130 140 150 160 170 180 GDQGIMFGYA TDETEEAMPL TLLLSHKLNR KLHELRRSGE LEWVRPDSKT QVTIEYASEG 190 200 210 220 230 240 GACVPLRVHT VVISTQHSPD ISLDDLRKEL IEKVIKAVIP ANLIDDKTIY HLNPCGSFII 250 260 270 280 290 300 GGPMGDAGLT GRKIIVDTYG GWGAHGGGAF SGKDPTKVDR SAAYAARWVA KSLVKAGLCR 310 320 330 340 350 360 RCLVQVSYAI GVAKPLSVMV FSFGTSALSE EDLLTIVNDN FDLRPGKIIK NLDLKRPIYE 370 380 390 400 PTAENGHFGH NNFPWEQPRN LKISADMLAK SQGPAQPDVI GIAH

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U41009 Genomic DNA. Translation: AAA82279.1.
PIR	T34084.
RefSeq	NP_500871.1.
UniGene	Cel.17523
3D structure databases
HSSP	HSSP built from PDB template 1QM4 based on UniProtKB P13444.
SMR	P50306. Positions 6-382.
ModBase	Search...
Protein-protein interaction databases
IntAct	P50306. 1 interaction.
STRING	P50306.
Proteomic databases
PRIDE	P50306.
Genome annotation databases
Ensembl	C06E7.3a; C06E7.3a; C06E7.3; Caenorhabditis elegans. [Genome view]
GeneID	177354.
KEGG	cel:C06E7.3.
UCSC	C06E7.3b.1. c. elegans.
Organism-specific databases
CTD	177354.
WormBase	WBGene00015540. sams-4.
WormPep	C06E7.3. CE03959. [WorfDB]
Phylogenomic databases
OMA	PWEVPKN.
Enzyme and pathway databases
BRENDA	2.5.1.6. 672.
Gene expression databases
ArrayExpress	P50306.
Family and domain databases
InterPro	IPR002133. S-AdoMet_synthetase. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHETASE_1. 1 hit. PS00377. ADOMET_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	896416.

Entry information

Entry name

METK4_CAEEL

Accession

Primary (citable) accession number: P50306

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project