Reviewed, UniProtKB/Swiss-Prot P50298 (ARY2_RAT)

Names and origin

Protein names

Recommended name:
    Arylamine N-acetyltransferase 2
    EC=2.3.1.5
Alternative name(s):
    Arylamide acetylase 2
    N-acetyltransferase type 2
      Short name=NAT-2
      Short name=AT-2

Gene names

Name:	Nat2
Synonyms:	Aac2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Acetylates only arylamines.
Catalytic activity	Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.
Subcellular location	Cytoplasm.
Polymorphism	There are two forms of NAT2: a rapid NAT2*21A) and a slow NAT2*21B).
Sequence similarities	Belongs to the arylamine N-acetyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	gut development Inferred from expression pattern. Source: RGD metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arylamine N-acetyltransferase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Arylamine N-acetyltransferase 2		PRO_0000107912
Regions
	Region	106 – 107	2	Substrate binding By similarity
Sites
	Active site	68	1	Acyl-thioester intermediate By similarity
	Active site	107	1	By similarity
	Active site	122	1	By similarity
	Binding site	103	1	Coenzyme A By similarity
	Binding site	104	1	Coenzyme A; via amide nitrogen By similarity
	Binding site	208	1	Coenzyme A By similarity
Amino acid modifications
	Modified residue	246	1	Phosphotyrosine By similarity
Natural variations
	Natural variant	121	1	V → I in allele NAT2*21; slow acetylator.
	Natural variant	266	1	V → I in allele NAT2*21; slow acetylator.

Sequences

	Sequence		Length	Mass (Da)
	P50298-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A02BF839A230F84D	FASTA	290	33,756
	10 20 30 40 50 60 MDIEAYFERI GYQSSRNKLD LEELTEILQH QIRAIPFENL NIHCGESMEL NLEVIFDQVV 70 80 90 100 110 120 RKKRGGWCLQ VNHLLYWALT KMGFEATMLG GYVFNTPANK YSSGMIHLLV QVTLSGKDYI 130 140 150 160 170 180 VDAGFGRSYQ MWEPLELTSG KDQPQVPAIF RLTEENGTWY LDQIRREQYV PNQEFVNSDL 190 200 210 220 230 240 LEKNKYRKIY SFTLEPRTIE DFESINTYLQ TSPASLFTSK SFCSLQTLEG VHCLVGSTLT 250 260 270 280 290 YRRFSYKDNI DLVEFKSLTE EEIEDVLKTI FGVSLERKLV PKHGDRFFTI

References

[1]	"Complementary DNAs for two arylamine N-acetyltransferases with identical 5' non-coding regions from rat pineal gland." Ebisawa T., Sasaki Y., Deguchi T. Eur. J. Biochem. 228:129-137(1995) [PubMed: 7882993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Pineal gland.
[2]	"Cloning, sequencing and expression of NAT1 and NAT2 encoding genes from rapid and slow acetylator inbred rats." Doll M.A., Hein D.W. Pharmacogenetics 5:247-251(1995) [PubMed: 8528272] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WKY/NCRLBR. Tissue: Heart.
[3]	"Recombinant rat and hamster acetyltransferases-1 and -2: relative rates of N-acetylation of arylamines and N,O-acyltransfer with arylhydroxamic acids." Jones R.F., Gott B., Land S.J., Park J., King C.M. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Sprague-Dawley. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U01348 mRNA. Translation: AAA70161.1. U23418 Genomic DNA. Translation: AAB53956.1. U19272 Genomic DNA. Translation: AAB60501.1. U17261 mRNA. Translation: AAA56772.1.
IPI	IPI00196632.
PIR	I58425.
RefSeq	NP_446306.1.
UniGene	Rn.198370 Rn.37420
3D structure databases
SMR	P50298. Positions 2-290.
ModBase	Search...
Protein-protein interaction databases
STRING	P50298.
PTM databases
PhosphoSite	P50298.
Genome annotation databases
Ensembl	ENSRNOT00000044198; ENSRNOP00000051430; ENSRNOG00000014055; Rattus norvegicus. [Genome view]
GeneID	116632.
KEGG	rno:116632.
UCSC	NM_053854. rat.
Organism-specific databases
CTD	116632.
RGD	70492. Nat2.
Phylogenomic databases
HOVERGEN	P50298.
InParanoid	P50298.
PhylomeDB	P50298.
Enzyme and pathway databases
BRENDA	2.3.1.5. 248. 2.3.1.56. 248.
Gene expression databases
ArrayExpress	P50298.
Genevestigator	P50298.
GermOnline	ENSRNOG00000014055. Rattus norvegicus.
Family and domain databases
InterPro	IPR001447. N-AcTrfase. [Graphical view]
PANTHER	PTHR11786. Acetyltransf2. 1 hit.
Pfam	PF00797. Acetyltransf_2. 1 hit. [Graphical view]
PRINTS	PR01543. ANATRNSFRASE.
ProtoNet	Search...
Other Resources
NextBio	619329.

Entry information

Entry name

ARY2_RAT

Accession

Primary (citable) accession number: P50298

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 19, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)