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Protein

Arylamine N-acetyltransferase 2

Gene

Nat2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Acetylates only arylamines.

Catalytic activityi

Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei68Acyl-thioester intermediateBy similarity1
Binding sitei103Coenzyme ABy similarity1
Binding sitei104Coenzyme A; via amide nitrogenBy similarity1
Active sitei107By similarity1
Active sitei122By similarity1
Binding sitei208Coenzyme ABy similarity1

GO - Molecular functioni

  • arylamine N-acetyltransferase activity Source: RGD
  • N-acetyltransferase activity Source: RGD

GO - Biological processi

  • digestive tract development Source: RGD
  • metabolic process Source: InterPro
  • response to hypoxia Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to xenobiotic stimulus Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.56. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Arylamine N-acetyltransferase 2 (EC:2.3.1.5)
Alternative name(s):
Arylamide acetylase 2
N-acetyltransferase type 2
Short name:
AT-2
Short name:
NAT-2
Gene namesi
Name:Nat2
Synonyms:Aac2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70492. Nat2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001079121 – 290Arylamine N-acetyltransferase 2Add BLAST290

Proteomic databases

PaxDbiP50298.
PRIDEiP50298.

PTM databases

iPTMnetiP50298.
PhosphoSitePlusiP50298.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000051430.

Structurei

3D structure databases

ProteinModelPortaliP50298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni106 – 107Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFD6. Eukaryota.
COG2162. LUCA.
HOGENOMiHOG000205435.
HOVERGENiHBG000165.
InParanoidiP50298.
KOiK00622.

Family and domain databases

InterProiIPR001447. Arylamine_N-AcTrfase.
[Graphical view]
PANTHERiPTHR11786. PTHR11786. 1 hit.
PfamiPF00797. Acetyltransf_2. 1 hit.
[Graphical view]
PRINTSiPR01543. ANATRNSFRASE.

Sequencei

Sequence statusi: Complete.

P50298-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIEAYFERI GYQSSRNKLD LEELTEILQH QIRAIPFENL NIHCGESMEL
60 70 80 90 100
NLEVIFDQVV RKKRGGWCLQ VNHLLYWALT KMGFEATMLG GYVFNTPANK
110 120 130 140 150
YSSGMIHLLV QVTLSGKDYI VDAGFGRSYQ MWEPLELTSG KDQPQVPAIF
160 170 180 190 200
RLTEENGTWY LDQIRREQYV PNQEFVNSDL LEKNKYRKIY SFTLEPRTIE
210 220 230 240 250
DFESINTYLQ TSPASLFTSK SFCSLQTLEG VHCLVGSTLT YRRFSYKDNI
260 270 280 290
DLVEFKSLTE EEIEDVLKTI FGVSLERKLV PKHGDRFFTI
Length:290
Mass (Da):33,756
Last modified:October 1, 1996 - v1
Checksum:iA02BF839A230F84D
GO

Polymorphismi

There are two forms of NAT2: a rapid isoform (NAT2*21A) and a slow isoform (NAT2*21B).

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti121V → I in allele NAT2*21; slow acetylator. 1 Publication1
Natural varianti266V → I in allele NAT2*21; slow acetylator. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01348 mRNA. Translation: AAA70161.1.
U23418 Genomic DNA. Translation: AAB53956.1.
U19272 Genomic DNA. Translation: AAB60501.1.
U17261 mRNA. Translation: AAA56772.1.
PIRiI58425.
RefSeqiNP_446306.1. NM_053854.1.
UniGeneiRn.37420.

Genome annotation databases

GeneIDi116632.
KEGGirno:116632.
UCSCiRGD:70492. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01348 mRNA. Translation: AAA70161.1.
U23418 Genomic DNA. Translation: AAB53956.1.
U19272 Genomic DNA. Translation: AAB60501.1.
U17261 mRNA. Translation: AAA56772.1.
PIRiI58425.
RefSeqiNP_446306.1. NM_053854.1.
UniGeneiRn.37420.

3D structure databases

ProteinModelPortaliP50298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000051430.

PTM databases

iPTMnetiP50298.
PhosphoSitePlusiP50298.

Proteomic databases

PaxDbiP50298.
PRIDEiP50298.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi116632.
KEGGirno:116632.
UCSCiRGD:70492. rat.

Organism-specific databases

CTDi10.
RGDi70492. Nat2.

Phylogenomic databases

eggNOGiENOG410IFD6. Eukaryota.
COG2162. LUCA.
HOGENOMiHOG000205435.
HOVERGENiHBG000165.
InParanoidiP50298.
KOiK00622.

Enzyme and pathway databases

BRENDAi2.3.1.56. 5301.

Miscellaneous databases

PROiP50298.

Family and domain databases

InterProiIPR001447. Arylamine_N-AcTrfase.
[Graphical view]
PANTHERiPTHR11786. PTHR11786. 1 hit.
PfamiPF00797. Acetyltransf_2. 1 hit.
[Graphical view]
PRINTSiPR01543. ANATRNSFRASE.
ProtoNetiSearch...

Entry informationi

Entry nameiARY2_RAT
AccessioniPrimary (citable) accession number: P50298
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.