P50298 (ARY2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arylamine N-acetyltransferase 2
EC=2.3.1.5

Alternative name(s):
Arylamide acetylase 2
N-acetyltransferase type 2
Short name=AT-2
Short name=NAT-2

Gene names

Name:	Nat2
Synonyms:	Aac2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Acetylates only arylamines.
Catalytic activity	Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.
Subcellular location	Cytoplasm.
Polymorphism	There are two forms of NAT2: a rapid NAT2*21A) and a slow NAT2*21B).
Sequence similarities	Belongs to the arylamine N-acetyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	digestive tract development Inferred from expression pattern PubMed 18799802. Source: RGD response to lipopolysaccharide Inferred from expression pattern PubMed 12071337. Source: RGD response to xenobiotic stimulus Inferred from expression pattern PubMed 18842621. Source: RGD
Cellular_component	cytosol Inferred from direct assay PubMed 16192314. Source: RGD
Molecular_function	arylamine N-acetyltransferase activity Inferred from direct assay Ref.1PubMed 8761433. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Arylamine N-acetyltransferase 2		PRO_0000107912
Regions
	Region	106 – 107	2	Substrate binding By similarity
Sites
	Active site	68	1	Acyl-thioester intermediate By similarity
	Active site	107	1	By similarity
	Active site	122	1	By similarity
	Binding site	103	1	Coenzyme A By similarity
	Binding site	104	1	Coenzyme A; via amide nitrogen By similarity
	Binding site	208	1	Coenzyme A By similarity
Amino acid modifications
	Modified residue	246	1	Phosphotyrosine By similarity
Natural variations
	Natural variant	121	1	V → I in allele NAT2*21; slow acetylator.
	Natural variant	266	1	V → I in allele NAT2*21; slow acetylator.

Sequences

	Sequence		Length	Mass (Da)
	P50298 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A02BF839A230F84D	FASTA	290	33,756
	10 20 30 40 50 60 MDIEAYFERI GYQSSRNKLD LEELTEILQH QIRAIPFENL NIHCGESMEL NLEVIFDQVV 70 80 90 100 110 120 RKKRGGWCLQ VNHLLYWALT KMGFEATMLG GYVFNTPANK YSSGMIHLLV QVTLSGKDYI 130 140 150 160 170 180 VDAGFGRSYQ MWEPLELTSG KDQPQVPAIF RLTEENGTWY LDQIRREQYV PNQEFVNSDL 190 200 210 220 230 240 LEKNKYRKIY SFTLEPRTIE DFESINTYLQ TSPASLFTSK SFCSLQTLEG VHCLVGSTLT 250 260 270 280 290 YRRFSYKDNI DLVEFKSLTE EEIEDVLKTI FGVSLERKLV PKHGDRFFTI

References

[1]	"Complementary DNAs for two arylamine N-acetyltransferases with identical 5' non-coding regions from rat pineal gland." Ebisawa T., Sasaki Y., Deguchi T. Eur. J. Biochem. 228:129-137(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Pineal gland.
[2]	"Cloning, sequencing and expression of NAT1 and NAT2 encoding genes from rapid and slow acetylator inbred rats." Doll M.A., Hein D.W. Pharmacogenetics 5:247-251(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WKY/NCRLBR. Tissue: Heart.
[3]	"Recombinant rat and hamster acetyltransferases-1 and -2: relative rates of N-acetylation of arylamines and N,O-acyltransfer with arylhydroxamic acids." Jones R.F., Gott B., Land S.J., Park J., King C.M. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Sprague-Dawley. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U01348 mRNA. Translation: AAA70161.1. U23418 Genomic DNA. Translation: AAB53956.1. U19272 Genomic DNA. Translation: AAB60501.1. U17261 mRNA. Translation: AAA56772.1.
IPI	IPI00196632.
PIR	I58425.
RefSeq	NP_446306.1. NM_053854.1.
UniGene	Rn.37420.
3D structure databases
ProteinModelPortal	P50298.
SMR	P50298. Positions 2-290.
ModBase	Search...
PTM databases
PhosphoSite	P50298.
Proteomic databases
PRIDE	P50298.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	116632.
KEGG	rno:116632.
UCSC	RGD:70492. rat.
Organism-specific databases
CTD	10.
RGD	70492. Nat2.
Phylogenomic databases
HOGENOM	HOG000205435.
HOVERGEN	HBG000165.
InParanoid	P50298.
KO	K00622.
OrthoDB	EOG46Q6T2.
Enzyme and pathway databases
BRENDA	2.3.1.56. 5301.
Gene expression databases
ArrayExpress	P50298.
Genevestigator	P50298.
GermOnline	ENSRNOG00000014055. Rattus norvegicus.
Family and domain databases
InterPro	IPR001447. Arylamine_N-AcTrfase. [Graphical view]
PANTHER	PTHR11786. PTHR11786. 1 hit.
Pfam	PF00797. Acetyltransf_2. 1 hit. [Graphical view]
PRINTS	PR01543. ANATRNSFRASE.
ProtoNet	Search...
Other
NextBio	619329.

Entry information

Entry name

ARY2_RAT

Accession

Primary (citable) accession number: P50298

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program