Reviewed, UniProtKB/Swiss-Prot P50297 (ARY1_RAT)

Names and origin

Protein names

Recommended name:
    Arylamine N-acetyltransferase 1
    EC=2.3.1.5
Alternative name(s):
    Arylamide acetylase 1
    N-acetyltransferase type 1
      Short name=NAT-1
      Short name=AT-1

Gene names

Name:	Nat1
Synonyms:	Aac1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Acetylates both arylamines and arylalkylamines.
Catalytic activity	Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the arylamine N-acetyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	liver development Inferred from expression pattern. Source: RGD metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arylamine N-acetyltransferase activity Inferred from direct assay. Source: RGD translation repressor activity Traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Arylamine N-acetyltransferase 1		PRO_0000107911
Regions
	Region	106 – 107	2	Substrate binding By similarity
Sites
	Active site	68	1	Acyl-thioester intermediate By similarity
	Active site	107	1	By similarity
	Active site	122	1	By similarity
	Binding site	103	1	Coenzyme A By similarity
	Binding site	208	1	Coenzyme A By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50297-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 2897CC1F84B7D72A	FASTA	290	33,437
	10 20 30 40 50 60 MDIEAYFERI GYKNSVNKLD LATLTEVLQH QMRAVPFENL SMHCGEAMCL GLEATFDHIV 70 80 90 100 110 120 RKKRGGWCLQ VNHLLYWALT KMGFETTMLG GYVYITPVNK YSSEMVHLLV QVTISDRNYI 130 140 150 160 170 180 VDSAYGSSYQ MWEPLELTSG KDQPQVPAIF RLTEENGTWY LDQIRREQDV PNQEFVNSDL 190 200 210 220 230 240 LEKSKYRKIY SFTLEPRTIE DFEYVNTYLQ TSPASVFVST SFCSLQTSEG VCCLIGSTLT 250 260 270 280 290 SRRFSYKDNV DLVEFKSLTE EEIEDVLKTT FGISLEKKFV PKHGELVFTI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complementary DNAs for two arylamine N-acetyltransferases with identical 5' non-coding regions from rat pineal gland." Ebisawa T., Sasaki Y., Deguchi T. Eur. J. Biochem. 228:129-137(1995) [PubMed: 7882993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Pineal gland.
[2]	"Recombinant rat and hamster acetyltransferases-1 and -2: relative rates of N-acetylation of arylamines and N,O-acyltransfer with arylhydroxamic acids." Jones R.F., Gott B., Land S.J., Park J., King C.M. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U01344 mRNA. Translation: AAA70157.1. U01343 mRNA. Translation: AAA70156.1. U17260 mRNA. Translation: AAA56771.1. U01345 mRNA. Translation: AAA70158.1. BC078765 mRNA. Translation: AAH78765.1.
IPI	IPI00196630.
PIR	I67465.
RefSeq	NP_001032392.1. NP_001032393.1. NP_446305.1.
UniGene	Rn.37420
3D structure databases
SMR	P50297. Positions 2-290.
ModBase	Search...
Protein-protein interaction databases
STRING	P50297.
PTM databases
PhosphoSite	P50297.
Genome annotation databases
Ensembl	ENSRNOT00000018854; ENSRNOP00000018854; ENSRNOG00000014055; Rattus norvegicus. [Genome view]
GeneID	116631.
KEGG	rno:116631.
UCSC	NM_053853. rat.
Organism-specific databases
CTD	116631.
RGD	70490. Nat1.
Phylogenomic databases
eggNOG	roNOG04860.
HOVERGEN	P50297.
OMA	AIFDHIV.
PhylomeDB	P50297.
Enzyme and pathway databases
BRENDA	2.3.1.5. 248. 2.3.1.56. 248.
Gene expression databases
ArrayExpress	P50297.
Genevestigator	P50297.
GermOnline	ENSRNOG00000014055. Rattus norvegicus.
Family and domain databases
InterPro	IPR001447. N-AcTrfase. [Graphical view]
PANTHER	PTHR11786. Acetyltransf2. 1 hit.
Pfam	PF00797. Acetyltransf_2. 1 hit. [Graphical view]
PRINTS	PR01543. ANATRNSFRASE.
ProtoNet	Search...
Other Resources
NextBio	619321.

Entry information

Entry name

ARY1_RAT

Accession

Primary (citable) accession number: P50297

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 19, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)